| 质粒名称: | pET-hisT4DNALig |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002246 |
| 产品用途: | active T4 DNA ligase |
| 存储实验室: | Po-Min Chiang Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pET3c |
| 表达系统: | 细菌表达 |
| 启动子: | T7 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Neb stable |
|
插入基因信息 |
|
| 插入基因名称: | T4 DNA ligase |
| 物种来源: | T4 phage |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
USPPAR is a cost-effective, scalable, and highly sensitive single-cell RNA sequencing workflow compatible with diverse specimens.
Hsueh, Y.-W. & Chiang, P.-M.
PLOS Biol. 23, e3003537 (2025)
|
买家导航
pET-hisT4DNALig载体质粒基本信息
pET-hisT4DNALig质粒图谱载体图谱和pET-hisT4DNALig载体序列质粒序列多克隆位点信息
pET-hisT4DNALig质粒载体简介
pET-hisT4DNALig质粒序列载体序列
LOCUS pET-hisT4DNALig 6098 bp DNA circular SYN 17-APR-2026
DEFINITION Expresses his-tagged T4 DNA ligase in E. coli hosts.
ACCESSION .
VERSION .
KEYWORDS pET-hisT4DNALig
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6098)
TITLE Bacterial expression plasmids for production of recombinant PNK
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 6098)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 21, 2026 from SnapGene 6.0.2
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
FEATURES Location/Qualifiers
source 1..6098
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 10..38
/label=tet promoter
/note="E. coli promoter for tetracycline efflux protein
gene"
terminator complement(404..451)
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
primer_bind 447..465
/label=T7 Term
/note="T7 terminator, reverse primer"
CDS complement(1992..2009)
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
RBS 2020..2042
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
primer_bind complement(2073..2092)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(2074..2092)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(2176..2195)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
CDS 3653..3844
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/label=rop
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
primer_bind complement(3838..3860)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
misc_feature 3946..4085
/label=bom
/note="basis of mobility region from pBR322"
primer_bind 4001..4020
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(4100..4117)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4271..4859)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4351..4370)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5030..5890)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5653..5672
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5891..5995)
/gene="bla"
/label=AmpR promoter
primer_bind 6063..6081
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
ORIGIN
1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
361 cacacccgtc ctgtggatat ccggatatag ttcctccttt cagcaaaaaa cccctcaaga
421 cccgtttaga ggccccaagg ggttatgcta gttattgctc agcggtggca gcagccaact
481 cagcttcctt tcgggctttg ttagcagccg gatccgcata ggccttatag accagttacc
541 tcatgaaaat caccaaatac atcttcgaat gtattagctt tagttttatc ttcacgtaaa
601 cgaatcgcaa tcggaagaaa taatttaacg taatcagtgc ggccatcaga ttttaaccaa
661 ccgttgcatt cgcactctag aatttttcca atataataat tttggttttc cataatgcga
721 gtacggtcaa gttcatgcga ttttacaccg gctttatctt ttaagcctga accagcattt
781 accttaattt ttccacactc tgactcaaga ataaatccac ccgctttagt agggtcttta
841 cggtgaggat aaattcctac aatttttaaa tcaacatcaa ttacttcttt aaatttataa
901 agattttttg aacgagcatt ttcccataat ccatcgatat ttttgagaat aataccttca
961 agaccttggt caatatactt tttataaatt accttagctt catctaggtt atttactacc
1021 tggttttcaa ttaaaattac tttatcatat ccagatgtca tttgttctag tttagaaaaa
1081 cgtacatcat atttcaaacg aaatgcagga agactgtata tttctaccaa cgggacataa
1141 tcccagacct gaaacttcat gcattgtgct tctttttcag aaatggttcc ctttaaagat
1201 ttattggcga ttccattaga agcagtacgt gattcagcaa cttcggcgaa ttctttagct
1261 ttactgtttt caggataagc atcaaaaaga aaatctaggc cttctggctc ctttttaact
1321 tgctcatggt ataccaattc gccatcaatc aacacacctt ctggatgaat ctggcgggct
1381 tcagcggtca ttttaattaa ctcttcctta agaagatcta atcctagata ttcattacca
1441 gctcgtgata aaagacgaac atcatctaat tcatcacctc taacttcagc aaaacaccga
1501 gctccatcag cttttaactg agcaaaggct ggaaatttga tattcttatt aatgcctttt
1561 tcatcataag aacttgcgag catttgaggt tgttcaggaa ttaaacctgg ccaaactttg
1621 tttgcaatag atactgaagc accacattca aggtctcgca tcatcactcg acgcaaaact
1681 tcaacatcat cttttttacc atcggtgata tatccagtta attcctcaat tgctgcattt
1741 ccagtcaatt tccgagtagc taatgtgaat tcaatgaagt caagcatatc ggtaagagtc
1801 aacattccaa aactctgggt agcaatacca ggtttaggcc atttcttgat ataatactgt
1861 aacccacgag aataagtcag acgatatact cgtttaagca attcattatc tttattcttt
1921 tcaagaattg cttgcttctg tttagttgaa ccaatagatg ctatttcgtt cagaatttta
1981 agaatgccgc tgtgatggtg atggtggtgc atatgtatat ctccttctta aagttaaaca
2041 aaattatttc tagagggaaa ccgttgtggt ctccctatag tgagtcgtat taatttcgcg
2101 ggatcgagat ctcgatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag
2161 gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact
2221 tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac
2281 tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca
2341 acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgaccgatgc
2401 ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg
2461 ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct
2521 gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg
2581 cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac
2641 gtttcggcga gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct
2701 tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg
2761 gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc
2821 agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc attggaccgc
2881 tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg
2941 taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg
3001 ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcacca ctccaagaat
3061 tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt ggcagaacat
3121 atccatcgcg tccgccatct ccagcagccg cacgcggcgc atctcgggca gcgttgggtc
3181 ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg
3241 ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg
3301 ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta
3361 aagtctggaa acgcggaagt cagcgccctg caccattatg ttccggatct gcatcgcagg
3421 atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct ggcattgacc
3481 ctgagtgatt tttctctggt cccgccgcat ccataccgcc agttgtttac cctcacaacg
3541 ttccagtaac cgggcatgtt catcatcagt aacccgtatc gtgagcatcc tctctcgttt
3601 catcggtatc attaccccca tgaacagaaa tcccccttac acggaggcat cagtgaccaa
3661 acaggaaaaa accgccctta acatggcccg ctttatcaga agccagacat taacgcttct
3721 ggagaaactc aacgagctgg acgcggatga acaggcagac atctgtgaat cgcttcacga
3781 ccacgctgat gagctttacc gcagctgcct cgcgcgtttc ggtgatgacg gtgaaaacct
3841 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
3901 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca
3961 gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta
4021 ctgagagtgc accattgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
4081 tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
4141 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
4201 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
4261 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
4321 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
4381 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
4441 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
4501 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
4561 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
4621 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
4681 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
4741 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
4801 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
4861 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
4921 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
4981 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
5041 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
5101 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
5161 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
5221 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
5281 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
5341 ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
5401 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
5461 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
5521 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
5581 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
5641 cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
5701 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
5761 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
5821 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
5881 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
5941 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
6001 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
6061 aaaataggcg tatcacgagg ccctttcgtc ttcaagaa
//
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