pH-ePPE

LOCUS       pH-ePPE      19641 bp DNA     circular SYN 25-AUG-2024
DEFINITION  For plant prime editing in rice plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19641)
  AUTHORS   Zong Y, Liu Y, Xue C, Li B, Li X, Wang Y, Li J, Liu G, Huang X, Cao 
            X, Gao C
  TITLE     An engineered prime editor with enhanced editing efficiency in 
            plants.
  JOURNAL   Nat Biotechnol. 2022 Mar 24. doi: 10.1038/s41587-022-01254-w.
  PUBMED    35332341
REFERENCE   2  (bases 1 to 19641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 25, 2024 from SnapGene Server 7.0.3
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Mar 24. doi: 10.1038/s41587-022-01254-w."
FEATURES             Location/Qualifiers
     source          1..19641
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    199..223
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     411..433
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     425..442
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     426..442
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        505..884
                     /label=OsU3 promoter
                     /note="Oryza sativa (rice) snRNA U3 promoter"
     CDS             1164..1955
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_RNA        2107..2182
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        2486..4477
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             4488..4508
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4509..8609
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             8522..8530
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             8832..8852
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             10515..10535
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(11194..11210)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11194..11210)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11207..11229)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11218..11234
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(11242..11272)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11287..11308
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        11499..12175
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     12112..12132
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             12242..13267
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    13308..13482
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    13560..13584
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             14009..14803
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      14890..15478
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15379..15398
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     15632..15649
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    15664..15804
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(15730..15749)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     15890..15912
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      16148..16342
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(16408..17481)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(17910..18539)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 cgacatgtcg ttttcagaag acggctgcac tgaacgtcag aagccgactg cactatagca
       61 gcggaggggt tggatcaaag tactttgatc ccgaggggaa ccctgtggtt ggcatgcaca
      121 tacaaatgga cgaacggata aaccttttca cgccctttta aatatccgat tattctaata
      181 aacgctcttt tctcttaggt ttacccgcca atatatcctg tcaaacactg atagtttaaa
      241 ctgaaggcgg gaaacgacaa tctgatccaa gctcaagctg ctctagcatt cgccattcag
      301 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
      361 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
      421 acgttgtaaa acgacggcca gtgccatagt aattcatcca ggtctccaag ttctaggatt
      481 ttcagaactg caacttattt tatcaaggaa tctttaaaca tacgaacaga tcacttaaag
      541 ttcttctgaa gcaacttaaa gttatcaggc atgcatggat cttggaggaa tcagatgtgc
      601 agtcagggac catagcacaa gacaggcgtc ttctactggt gctaccagca aatgctggaa
      661 gccgggaaca ctgggtacgt tggaaaccac gtgatgtgaa gaagtaagat aaactgtagg
      721 agaaaagcat ttcgtagtgg gccatgaagc ctttcaggac atgtattgca gtatgggccg
      781 gcccattacg caattggacg acaacaaaga ctagtattag taccacctcg gctatccaca
      841 tagatcaaag ctgatttaaa agagttgtgc agatgatccg tggcgtgaga ccaacccagt
      901 ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact
      961 ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt
     1021 gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt
     1081 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc
     1141 gccctaaaac aaagttaaac atcatggggg aagcggtgat cgccgaagta tcgactcaac
     1201 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     1261 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     1321 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     1381 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     1441 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     1501 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     1561 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     1621 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     1681 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     1741 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     1801 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
     1861 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg
     1921 tgaaaggcga gatcaccaag gtagtcggca aataatgtct agctagaaat tcgttcaagc
     1981 cgacgccgct tcgcggcgcg gcttaactca agcgttagat gcactaagca cataattgct
     2041 cacagccaaa ctatcaggtc aagtctgctt ttattatttt taagcgtgca taataagccg
     2101 gtctcggttt tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga
     2161 aaaagtggca ccgagtcggt gctttttttt ttcgttttgc attgagtttt ctccgtcgca
     2221 tgtttgcagt tttattttcc gttttgcatt gaaatttctc cgtctcatgt ttgcagcgtg
     2281 ttcaaaaagt acgcagctgt atttcactta tttacggcgc cacattttca tgccgtttgt
     2341 gccaactatc ccgagctagt gaatacagct tggcttcaca caacactggt gacccgctga
     2401 cctgctcgta cctcgtaccg tcgtacggca cagcatttgg aattaaaggg tgtgatcgat
     2461 actgcttgct gctaagcttg catgcctgca gtgcagcgtg acccggtcgt gcccctctct
     2521 agagataatg agcattgcat gtctaagtta taaaaaatta ccacatattt tttttgtcac
     2581 acttgtttga agtgcagttt atctatcttt atacatatat ttaaacttta ctctacgaat
     2641 aatataatct atagtactac aataatatca gtgttttaga gaatcatata aatgaacagt
     2701 tagacatggt ctaaaggaca attgagtatt ttgacaacag gactctacag ttttatcttt
     2761 ttagtgtgca tgtgttctcc tttttttttg caaatagctt cacctatata atacttcatc
     2821 cattttatta gtacatccat ttagggttta gggttaatgg tttttataga ctaatttttt
     2881 tagtacatct attttattct attttagcct ctaaattaag aaaactaaaa ctctatttta
     2941 gtttttttat ttaataattt agatataaaa tagaataaaa taaagtgact aaaaattaaa
     3001 caaataccct ttaagaaatt aaaaaaacta aggaaacatt tttcttgttt cgagtagata
     3061 atgccagcct gttaaacgcc gtcgacgagt ctaacggaca ccaaccagcg aaccagcagc
     3121 gtcgcgtcgg gccaagcgaa gcagacggca cggcatctct gtcgctgcct ctggacccct
     3181 ctcgagagtt ccgctccacc gttggacttg ctccgctgtc ggcatccaga aattgcgtgg
     3241 cggagcggca gacgtgagcc ggcacggcag gcggcctcct cctcctctca cggcaccggc
     3301 agctacgggg gattcctttc ccaccgctcc ttcgctttcc cttcctcgcc cgccgtaata
     3361 aatagacacc ccctccacac cctctttccc caacctcgtg ttgttcggag cgcacacaca
     3421 cacaaccaga tctcccccaa atccacccgt cggcacctcc gcttcaaggt acgccgctcg
     3481 tcctcccccc cccccntctc taccttctct agatcggcgt tccggtccat ggttagggcc
     3541 cggtagttct acttctgttc atgtttgtgt tagatccgtg tttgtgttag atccgtgctg
     3601 ctagcgttcg tacacggatg cgacctgtac gtcagacacg ttctgattgc taacttgcca
     3661 gtgtttctct ttggggaatc ctgggatggc tctagccgtt ccgcagacgg gatcgatttc
     3721 atgatttttt ttgtttcgtt gcatagggtt tggtttgccc ttttccttta tttcaatata
     3781 tgccgtgcac ttgtttgtcg ggtcatcttt tcatgctttt ttttgtcttg gttgtgatga
     3841 tgtggtctgg ttgggcggtc gttctagatc ggagtagaat tctgtttcaa actacctggt
     3901 ggatttatta attttggatc tgtatgtgtg tgccatacat attcatagtt acgaattgaa
     3961 gatgatggat ggaaatatcg atctaggata ggtatacatg ttgatgcggg ttttactgat
     4021 gcatatacag agatgctttt tgttcgcttg gttgtgatga tgtggtgtgg ttgggcggtc
     4081 gttcattcgt tctagatcgg agtagaatac tgtttcaaac tacctggtgt atttattaat
     4141 tttggaactg tatgtgtgtg tcatacatct tcatagttac gagtttaaga tggatggaaa
     4201 tatcgatcta ggataggtat acatgttgat gtgggtttta ctgatgcata tacatgatgg
     4261 catatgcagc atctattcat atgctctaac cttgagtacc tatctattat aataaacaag
     4321 tatgttttat aattattttg atcttgatat acttggatga tggcatatgc agcagctata
     4381 tgtggatttt tttagccctg ccttcatacg ctatttattt gcttggtact gtttcttttg
     4441 tcgatgctca ccctgttgtt tggtgttact tctgcagccc taggatgcct aagaaaaaga
     4501 gaaaagtgga caagaagtac tcgatcggcc tcgatattgg gactaactct gttggctggg
     4561 ccgtgatcac cgacgagtac aaggtgccct caaagaagtt caaggtcctg ggcaacaccg
     4621 atcggcattc catcaagaag aatctcattg gcgctctcct gttcgacagc ggcgagacgg
     4681 ctgaggctac gcggctcaag cgcaccgccc gcaggcggta cacgcgcagg aagaatcgca
     4741 tctgctacct gcaggagatt ttctccaacg agatggcgaa ggttgacgat tctttcttcc
     4801 acaggctgga ggagtcattc ctcgtggagg aggataagaa gcacgagcgg catccaatct
     4861 tcggcaacat tgtcgacgag gttgcctacc acgagaagta ccctacgatc taccatctgc
     4921 ggaagaagct cgtggactcc acagataagg cggacctccg cctgatctac ctcgctctgg
     4981 cccacatgat taagttcagg ggccatttcc tgatcgaggg ggatctcaac ccggacaata
     5041 gcgatgttga caagctgttc atccagctcg tgcagacgta caaccagctc ttcgaggaga
     5101 accccattaa tgcgtcaggc gtcgacgcga aggctatcct gtccgctagg ctctcgaagt
     5161 ctcggcgcct cgagaacctg atcgcccagc tgccgggcga gaagaagaac ggcctgttcg
     5221 ggaatctcat tgcgctcagc ctggggctca cgcccaactt caagtcgaat ttcgatctcg
     5281 ctgaggacgc caagctgcag ctctccaagg acacatacga cgatgacctg gataacctcc
     5341 tggcccagat cggcgatcag tacgcggacc tgttcctcgc tgccaagaat ctgtcggacg
     5401 ccatcctcct gtctgatatt ctcagggtga acaccgagat tacgaaggct ccgctctcag
     5461 cctccatgat caagcgctac gacgagcacc atcaggatct gaccctcctg aaggcgctgg
     5521 tcaggcagca gctccccgag aagtacaagg agatcttctt cgatcagtcg aagaacggct
     5581 acgctgggta cattgacggc ggggcctctc aggaggagtt ctacaagttc atcaagccga
     5641 ttctggagaa gatggacggc acggaggagc tgctggtgaa gctcaatcgc gaggacctcc
     5701 tgaggaagca gcggacattc gataacggca gcatcccaca ccagattcat ctcggggagc
     5761 tgcacgctat cctgaggagg caggaggact tctacccttt cctcaaggat aaccgcgaga
     5821 agatcgagaa gattctgact ttcaggatcc cgtactacgt cggcccactc gctaggggca
     5881 actcccgctt cgcttggatg acccgcaagt cagaggagac gatcacgccg tggaacttcg
     5941 aggaggtggt cgacaagggc gctagcgctc agtcgttcat cgagaggatg acgaatttcg
     6001 acaagaacct gccaaatgag aaggtgctcc ctaagcactc gctcctgtac gagtacttca
     6061 cagtctacaa cgagctgact aaggtgaagt atgtgaccga gggcatgagg aagccggctt
     6121 tcctgtctgg ggagcagaag aaggccatcg tggacctcct gttcaagacc aaccggaagg
     6181 tcacggttaa gcagctcaag gaggactact tcaagaagat tgagtgcttc gattcggtcg
     6241 agatctctgg cgttgaggac cgcttcaacg cctccctggg gacctaccac gatctcctga
     6301 agatcattaa ggataaggac ttcctggaca acgaggagaa tgaggatatc ctcgaggaca
     6361 ttgtgctgac actcactctg ttcgaggacc gggagatgat cgaggagcgc ctgaagactt
     6421 acgcccatct cttcgatgac aaggtcatga agcagctcaa gaggaggagg tacaccggct
     6481 gggggaggct gagcaggaag ctcatcaacg gcattcggga caagcagtcc gggaagacga
     6541 tcctcgactt cctgaagagc gatggcttcg cgaaccgcaa tttcatgcag ctgattcacg
     6601 atgacagcct cacattcaag gaggatatcc agaaggctca ggtgagcggc cagggggact
     6661 cgctgcacga gcatatcgcg aacctcgctg gctcgccagc tatcaagaag gggattctgc
     6721 agaccgtgaa ggttgtggac gagctggtga aggtcatggg caggcacaag cctgagaaca
     6781 tcgtcattga gatggcccgg gagaatcaga ccacgcagaa gggccagaag aactcacgcg
     6841 agaggatgaa gaggatcgag gagggcatta aggagctggg gtcccagatc ctcaaggagc
     6901 acccggtgga gaacacgcag ctgcagaatg agaagctcta cctgtactac ctccagaatg
     6961 gccgcgatat gtatgtggac caggagctgg atattaacag gctcagcgat tacgacgtcg
     7021 atgccatcgt tccacagtca ttcctgaagg atgactccat tgacaacaag gtcctcacca
     7081 ggtcggacaa gaaccggggc aagtctgata atgttccttc agaggaggtc gttaagaaga
     7141 tgaagaacta ctggcgccag ctcctgaatg ccaagctgat cacgcagcgg aagttcgata
     7201 acctcacaaa ggctgagagg ggcgggctct ctgagctgga caaggcgggc ttcatcaaga
     7261 ggcagctggt cgagacacgg cagatcacta agcacgttgc gcagattctc gactcacgga
     7321 tgaacactaa gtacgatgag aatgacaagc tgatccgcga ggtgaaggtc atcaccctga
     7381 agtcaaagct cgtctccgac ttcaggaagg atttccagtt ctacaaggtt cgggagatca
     7441 acaattacca ccatgcccat gacgcgtacc tgaacgcggt ggtcggcaca gctctgatca
     7501 agaagtaccc aaagctcgag agcgagttcg tgtacgggga ctacaaggtt tacgatgtga
     7561 ggaagatgat cgccaagtcg gagcaggaga ttggcaaggc taccgccaag tacttcttct
     7621 actctaacat tatgaatttc ttcaagacag agatcactct ggccaatggc gagatccgga
     7681 agcgccccct catcgagacg aacggcgaga cgggggagat cgtgtgggac aagggcaggg
     7741 atttcgcgac cgtcaggaag gttctctcca tgccacaagt gaatatcgtc aagaagacag
     7801 aggtccagac tggcgggttc tctaaggagt caattctgcc taagcggaac agcgacaagc
     7861 tcatcgcccg caagaaggac tgggatccga agaagtacgg cgggttcgac agccccactg
     7921 tggcctactc ggtcctggtt gtggcgaagg ttgagaaggg caagtccaag aagctcaaga
     7981 gcgtgaagga gctgctgggg atcacgatta tggagcgctc cagcttcgag aagaacccga
     8041 tcgatttcct ggaggcgaag ggctacaagg aggtgaagaa ggacctgatc attaagctcc
     8101 ccaagtactc actcttcgag ctggagaacg gcaggaagcg gatgctggct tccgctggcg
     8161 agctgcagaa ggggaacgag ctggctctgc cgtccaagta tgtgaacttc ctctacctgg
     8221 cctcccacta cgagaagctc aagggcagcc ccgaggacaa cgagcagaag cagctgttcg
     8281 tcgagcagca caagcattac ctcgacgaga tcattgagca gatttccgag ttctccaagc
     8341 gcgtgatcct ggccgacgcg aatctggata aggtcctctc cgcgtacaac aagcaccgcg
     8401 acaagccaat cagggagcag gctgagaata tcattcatct cttcaccctg acgaacctcg
     8461 gcgcccctgc tgctttcaag tacttcgaca caactatcga tcgcaagagg tacacaagca
     8521 ctaaggaggt cctggacgcg accctcatcc accagtcgat taccggcctc tacgagacgc
     8581 gcatcgacct gtctcagctc gggggcgacg aattctccgg gagcgagacg ccaggcacct
     8641 ccgagtcggc caccccagaa tctgccacag tggtgtccgg ccaaaagcag gaccgccagg
     8701 gcggagaacg cagaaggtcc cagctcgata gggatcagtg tgcctactgc aaggagaagg
     8761 gccactgggc caaagactgc ccgaaaaagc cgcgcggccc acgcggccca aggccacaaa
     8821 catccctcct tccaaagaag aagcggaagg tggagctcag cggaggatct tccggaggat
     8881 ctagcggctc cgagacacca ggaacatccg aaagcgctac accagaatct agcggaggct
     8941 cttccggagg atctaggcct accctcaaca tcgaggatga gtatcgcctc cacgaaacct
     9001 ccaaagaacc ggacgtgtcc ctcggcagca catggctcag cgacttccca caagcgtggg
     9061 ccgaaaccgg cggcatgggc ctcgccgtcc gccaagcccc actcattatc ccgctgaagg
     9121 cgacctccac accggtgtcc atcaagcagt acccgatgag ccaagaggcg aggctcggga
     9181 ttaagccgca cattcagcgc ctcctcgatc aaggcattct cgtgccgtgc caatccccgt
     9241 ggaatacacc actcctcccg gtcaaaaagc cgggcaccaa cgactatcgc ccggtccaag
     9301 atctccgcga ggtcaacaag cgcgtggaag acatccaccc gaccgtcccg aacccgtata
     9361 atctgctctc cgggctccca ccatcccacc agtggtatac agtgctggac ctcaaagacg
     9421 ccttcttctg tctccgcctc cacccaacaa gccagccgct cttcgccttc gagtggcgcg
     9481 acccggagat gggcatctcc ggccaactga catggacacg cctcccgcaa ggcttcaaga
     9541 acagcccgac actcttcaac gaggcgctcc atagggacct cgcggatttt cgcatccagc
     9601 atccggacct catcctcctc cagtatgtgg atgatctcct cctcgccgcg acctccgagc
     9661 tggattgtca acaaggcaca cgcgcgctcc tccaaacact cgggaacctc ggctatcgcg
     9721 cgtccgcgaa aaaggcccaa atctgccaga agcaagtgaa gtacctcggg tatctgctca
     9781 aggaaggcca acgctggctc accgaagcgc gcaaagaaac agtgatgggg caaccgacac
     9841 cgaaaacacc acgccagctg cgcgagtttc tcggcaaagc cggcttctgt cgcctcttca
     9901 tcccgggctt tgccgagatg gccgcgccac tctacccact caccaagccg ggcacactgt
     9961 ttaactgggg gccggatcag cagaaagcct accaagagat caaacaagcg ctcctcaccg
    10021 ccccagcgct cgggctccca gatctcacaa agccgttcga gctgttcgtc gatgagaagc
    10081 aaggctacgc gaagggcgtg ctcacacaga agctcggccc gtggaggagg ccagtggcct
    10141 atctctccaa aaaactcgat ccagtggccg ccggctggcc accgtgtctg cgcatggtcg
    10201 ccgcgattgc cgtgctcaca aaggatgccg gcaaactcac aatgggccag ccgctggtga
    10261 tcctcgcgcc acatgccgtg gaagccctcg tcaaacagcc gccggatagg tggctctcca
    10321 atgcgcgcat gacccattac caagcgctcc tcctcgacac cgatcgcgtc cagttcggcc
    10381 cagtggtcgc cctcaatccg gcgacactgc tgccactccc agaggagggc ctccaacaca
    10441 actgtctgga tattctcgcg gaagcgcatg gcacaaggcc agacctcaca gatcaaccgc
    10501 tcagcggcgg cagcccgaag aagaaaagga aggtgtgaga gctcagagct ttcgttcgta
    10561 tcatcggttt cgacaacgtt cgtcaagttc aatgcatcag tttcattgcg cacacaccag
    10621 aatcctactg agtttgagta ttatggcatt gggaaaactg tttttcttgt accatttgtt
    10681 gtgcttgtaa tttactgtgt tttttattcg gttttcgcta tcgaactgtg aaatggaaat
    10741 ggatggagaa gagttaatga atgatatggt ccttttgttc attctcaaat taatattatt
    10801 tgttttttct cttatttgtt gtgtgttgaa tttgaaatta taagagatat gcaaacattt
    10861 tgttttgagt aaaaatgtgt caaatcgtgg cctctaatga ccgaagttaa tatgaggagt
    10921 aaaacacttg tagttgtacc attatgctta ttcactaggc aacaaatata ttttcagacc
    10981 tagaaaagct gcaaatgtta ctgaatacaa gtatgtcctc ttgtgtttta gacatttatg
    11041 aactttcctt tatgtaattt tccagaatcc ttgtcagatt ctaatcattg ctttataatt
    11101 atagttatac tcatggattt gtagttgagt atgaaaatat tttttaatgc attttatgac
    11161 ttgccaattg attgacaacg aattcgtaat catgtcatag ctgtttcctg tgtgaaattg
    11221 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
    11281 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
    11341 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
    11401 gcgtattggc tagagcagct tgccaacatg gtggagcacg acactctcgt ctactccaag
    11461 aatatcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
    11521 atatcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat caaaaggaca
    11581 gtagaaaagg aaggtggcac ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt
    11641 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
    11701 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga acatggtgga
    11761 gcacgacact ctcgtctact ccaagaatat caaagataca gtctcagaag accaaagggc
    11821 tattgagact tttcaacaaa gggtaatatc gggaaacctc ctcggattcc attgcccagc
    11881 tatctgtcac ttcatcaaaa ggacagtaga aaaggaaggt ggcacctaca aatgccatca
    11941 ttgcgataaa ggaaaggcta tcgttcaaga tgcctctgcc gacagtggtc ccaaagatgg
    12001 acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca
    12061 agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc
    12121 gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggacac gctgaaatca
    12181 ccagtctctc tctacaaatc tatctctctc gagctttcgc agatccgggg ggcaatgaga
    12241 tatgaaaaag cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga
    12301 cagcgtctcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga
    12361 tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga
    12421 tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat
    12481 tggggagttt agcgagagcc tgacctattg catctcccgc cgttcacagg gtgtcacgtt
    12541 gcaagacctg cctgaaaccg aactgcccgc tgttctacaa ccggtcgcgg aggctatgga
    12601 tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg
    12661 aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta
    12721 tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga
    12781 gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg
    12841 ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc
    12901 gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc
    12961 ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc
    13021 acgactccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga
    13081 cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg
    13141 agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg
    13201 ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa
    13261 gaaatagagt agatgccgac cgggatctgt cgatcgacaa gctcgagttt ctccataata
    13321 atgtgtgagt agttcccaga taagggaatt agggttccta tagggtttcg ctcatgtgtt
    13381 gagcatataa gaaaccctta gtatgtattt gtatttgtaa aatacttcta tcaataaaat
    13441 ttctaattcc taaaaccaaa atccagtact aaaatccaga tcccccgaat taattcggcg
    13501 ttaattcagt acattaaaaa cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg
    13561 tttacaccac aatatatcct gccaccagcc agccaacagc tccccgaccg gcagctcggc
    13621 acaaaatcac cactcgatac aggcagccca tcagtccggg acggcgtcag cgggagagcc
    13681 gttgtaaggc ggcagacttt gctcatgtta ccgatgctat tcggaagaac ggcaactaag
    13741 ctgccgggtt tgaaacacgg atgatctcgc ggagggtagc atgttgattg taacgatgac
    13801 agagcgttgc tgcctgtgat caccgcggtt tcaaaatcgg ctccgtcgat actatgttat
    13861 acgccaactt tgaaaacaac tttgaaaaag ctgttttctg gtatttaagg ttttagaatg
    13921 caaggaacag tgaattggag ttcgtcttgt tataattagc ttcttggggt atctttaaat
    13981 actgtagaaa agaggaagga aataataaat ggctaaaatg agaatatcac cggaattgaa
    14041 aaaactgatc gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc ctgctaaggt
    14101 atataagctg gtgggagaaa atgaaaacct atatttaaaa atgacggaca gccggtataa
    14161 agggaccacc tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg aaggaaagct
    14221 gcctgttcca aaggtcctgc actttgaacg gcatgatggc tggagcaatc tgctcatgag
    14281 tgaggccgat ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc ctgaaaagat
    14341 tatcgagctg tatgcggagt gcatcaggct ctttcactcc atcgacatat cggattgtcc
    14401 ctatacgaat agcttagaca gccgcttagc cgaattggat tacttactga ataacgatct
    14461 ggccgatgtg gattgcgaaa actgggaaga agacactcca tttaaagatc cgcgcgagct
    14521 gtatgatttt ttaaagacgg aaaagcccga agaggaactt gtcttttccc acggcgacct
    14581 gggagacagc aacatctttg tgaaagatgg caaagtaagt ggctttattg atcttgggag
    14641 aagcggcagg gcggacaagt ggtatgacat tgccttctgc gtccggtcga tcagggagga
    14701 tatcggggaa gaacagtatg tcgagctatt ttttgactta ctggggatca agcctgattg
    14761 ggagaaaata aaatattata ttttactgga tgaattgttt tagtacctag aatgcatgac
    14821 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
    14881 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
    14941 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
    15001 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
    15061 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
    15121 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
    15181 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
    15241 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
    15301 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
    15361 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
    15421 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
    15481 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
    15541 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
    15601 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
    15661 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
    15721 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg
    15781 ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga
    15841 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
    15901 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg
    15961 gcgccggcgg tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat tgcctggccg
    16021 taggccagcc atttttgagc ggccagcggc cgcgataggc cgacgcgaag cggcggggcg
    16081 tagggagcgc agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc
    16141 agacagttat gcacaggcca ggcgggtttt aagagtttta ataagtttta aagagtttta
    16201 ggcggaaaaa tcgccttttt tctcttttat atcagtcact tacatgtgtg accggttccc
    16261 aatgtacggc tttgggttcc caatgtacgg gttccggttc ccaatgtacg gctttgggtt
    16321 cccaatgtac gtgctatcca caggaaacag accttttcga cctttttccc ctgctagggc
    16381 aatttgccct agcatctgct ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag
    16441 gttgcggtag cgcatgacta ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta
    16501 ctccggcagg tcatttgacc cgatcagctt gcgcacggtg aaacagaact tcttgaactc
    16561 tccggcgctg ccactgcgtt cgtagatcgt cttgaacaac catctggctt ctgccttgcc
    16621 tgcggcgcgg cgtgccaggc ggtagagaaa acggccgatg ccgggatcga tcaaaaagta
    16681 atcggggtga accgtcagca cgtccgggtt cttgccttct gtgatctcgc ggtacatcca
    16741 atcagctagc tcgatctcga tgtactccgg ccgcccggtt tcgctcttta cgatcttgta
    16801 gcggctaatc aaggcttcac cctcggatac cgtcaccagg cggccgttct tggccttctt
    16861 cgtacgctgc atggcaacgt gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc
    16921 tttctgcttt ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg
    16981 gaacacgcgg ccgggcttgt ctcccttccc ttcccggtat cggttcatgg attcggttag
    17041 atgggaaacc gccatcagta ccaggtcgta atcccacaca ctggccatgc cggccggccc
    17101 tgcggaaacc tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg
    17161 gtcacgcttc gacagacgga aaacggccac gtccatgatg ctgcgactat cgcgggtgcc
    17221 cacgtcatag agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct
    17281 aatcgacggc gcaccggctg ccggcggttg ccgggattct ttgcggattc gatcagcggc
    17341 cgcttgccac gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg
    17401 cgcggccttc aacttctcca ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc
    17461 ggatggtttg cgaccgctca cgccgattcc tcgggcttgg gggttccagt gccattgcag
    17521 ggccggcagg caacccagcc gcttacgcct ggccaaccgc ccgttcctcc acacatgggg
    17581 cattccacgg cgtcggtgcc tggttgttct tgattttcca tgccgcctcc tttagccgct
    17641 aaaattcatc tactcattta ttcatttgct catttactct ggtagctgcg cgatgtattc
    17701 agatagcagc tcggtaatgg tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg
    17761 atcctccgcc ggcaactgaa agttgacccg cttcatggct ggcgtgtctg ccaggctggc
    17821 caacgttgca gccttgctgc tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct
    17881 tttgctcatt ttctctttac ctcattaact caaatgagtt ttgatttaat ttcagcggcc
    17941 agcgcctgga cctcgcgggc agcgtcgccc tcgggttctg attcaagaac ggttgtgccg
    18001 gcggcggcag tgcctgggta gctcacgcgc tgcgtgatac gggactcaag aatgggcagc
    18061 tcgtacccgg ccagcgcctc ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc
    18121 gacacgacaa aggccgcttg tagccttcca tccgtgacct caatgcgctg cttaaccagc
    18181 tccaccaggt cggcggtggc ccatatgtcg taagggcttg gctgcaccgg aatcagcacg
    18241 aagtcggctg ccttgatcgc ggacacagcc aagtccgccg cctggggcgc tccgtcgatc
    18301 actacgaagt cgcgccggcc gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg
    18361 ccgacaacgg ttagcggttg atcttcccgc acggccgccc aatcgcgggc actgccctgg
    18421 ggatcggaat cgactaacag aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg
    18481 gttgcgatgg tcgtcttgcc tgacccgcct ttctggttaa gtacagcgat aaccttcatg
    18541 cgttcccctt gcgtatttgt ttatttactc atcgcatcat atacgcagcg accgcatgac
    18601 gcaagctgtt ttactcaaat acacatcacc tttttagacg gcggcgctcg gtttcttcag
    18661 cggccaagct ggccggccag gccgccagct tggcatcaga caaaccggcc aggatttcat
    18721 gcagccgcac ggttgagacg tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct
    18781 ccgcctcgat ctcttcggta atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca
    18841 tgcttgttcc tcttggcgtt cattctcggc ggccgccagg gcgtcggcct cggtcaatgc
    18901 gtcctcacgg aaggcaccgc gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc
    18961 aagtgcgcgg tacagggtcg agcgatgcac gccaagcagt gcagccgcct ctttcacggt
    19021 gcggccttcc tggtcgatca gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg
    19081 gcgggggcca aacttcacgc ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc
    19141 gatgattagg gaacgctcga actcggcaat gccggcgaac acggtcaaca ccatgcggcc
    19201 ggccggcgtg gtggtgtcgg cccacggctc tgccaggcta cgcaggcccg cgccggcctc
    19261 ctggatgcgc tcggcaatgt ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct
    19321 ggtcactgtc acaacgtcgc cagggcgtag gtggtcaagc atcctggcca gctccgggcg
    19381 gtcgcgcctg gtgccggtga tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag
    19441 ttcggcccgt tggttggtca agtcctggtc gtcggtgctg acgcgggcat agcccagcag
    19501 gccagcggcg gcgctcttgt tcatggcgta atgtctccgg ttctagtcgc aagtattcta
    19561 ctttatgcga ctaaaacacg cgacaagaaa acgccaggaa aagggcaggg cggcagcctg
    19621 tcgcgtaact taggacttgt g
//