| 质粒名称: | pGL3-SETBP1-Nicking/EpegRNA-PGK-puroR-mRFP1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002198 |
| 产品用途: | The hCtRNA simultaneously drives the expression of both the targeting SETBP1 gene NickingRNA and EpegRNA, harboring dual selection markers of puroR and RFP. |
| 存储实验室: | Zhuobin Liang Lab 梁卓斌课题组 |
|
载体骨架基本信息 |
|
| 载体名称: | pGL3 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | hCtRNA |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | SETBP1-nicking-sgRNA-EpegRNA |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
TRU-PE: A Universal, Trackable Prime Editor Toolkit for Robust Single-and Multi-Locus Genome Engineering
Zhichao Qiu, Keke Sun, Qingwei Zeng, Ziwei Luo, Xinran Liu, Yaping Li, Xiang Lei, Ruilin Zhao, Zhen Zhang, Damia Romero-Moya, Yuan Yang, Xuan Wang, Ehsan Hashemi, Joe Z. Zhang, Xiaolong Wang, Alessandra Giorgetti, Zhuobin Liang
bioRxiv 2026.02.20.706938
|
pGL3-SETBP1-Nicking/EpegRNA-PGK-puroR-mRFP1
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pGL3-SETBP1-Nicking/EpegRNA-PGK-puroR-mRFP1载体质粒基本信息
pGL3-SETBP1-Nicking/EpegRNA-PGK-puroR-mRFP1质粒图谱载体图谱和pGL3-SETBP1-Nicking/EpegRNA-PGK-puroR-mRFP1载体序列质粒序列多克隆位点信息
pGL3-SETBP1-Nicking/EpegRNA-PGK-puroR-mRFP1质粒载体简介
pGL3-SETBP1-Nicking/EpegRNA-PGK-puroR-mRFP1质粒序列载体序列
LOCUS pGL3-SETBP1-Nicking/EpegRNA-PGK-puroR-mRFP1 5718 bp DNA circular SYN 07-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5718)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 20, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5718
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 1..20
/label=OL2714
primer_bind 4..23
/label=OL1915
primer_bind 4..21
/label=OL1400
primer_bind 12..36
/label=OL2631
primer_bind complement(12..36)
/label=OL2637
primer_bind 17..48
/label=OL2785
primer_bind complement(17..44)
/label=OL2746
primer_bind 18..40
/label=OL2707
primer_bind 21..55
/label=OL2640
primer_bind 24..61
/label=OL2745
primer_bind 37..61
/label=OL1451
misc_feature 41..115
/label=hCtRNA
primer_bind 41..115
/label=OL477
primer_bind complement(98..131)
/label=OL2600
primer_bind complement(98..115)
/label=OL2595
primer_bind complement(98..115)
/label=OL2596
primer_bind complement(98..115)
/label=OL2602
primer_bind complement(98..115)
/label=OL2606
primer_bind complement(98..115)
/label=OL2608
misc_feature 116..135
/label=SETBP1 nickingRNA
primer_bind 119..171
/label=OL2601
primer_bind 133..171
/label=OL2607
primer_bind 135..171
/label=OL2609
primer_bind complement(136..215)
/label=OL476
misc_RNA 136..211
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
primer_bind 136..207
/label=OL2116
primer_bind 136..171
/label=OL2597
primer_bind 136..171
/label=OL2603
primer_bind complement(140..211)
/label=OL2117
primer_bind 140..177
/label=OL1453
primer_bind complement(183..202)
/label=OL1927
primer_bind complement(188..207)
/label=OL1940
primer_bind complement(194..212)
/label=OL2619
primer_bind complement(194..211)
/label=OL2615
primer_bind complement(194..211)
/label=OL2611
primer_bind complement(194..211)
/label=OL769
primer_bind complement(201..211)
/label=OL2116
primer_bind 208..286
/label=OL477
misc_feature 212..286
/label=hCtRNA
primer_bind 212..232
/label=OL1451
primer_bind 212..232
/label=OL2745
primer_bind 212..226
/label=OL2640
primer_bind complement(269..315)
/label=OL2595
primer_bind complement(269..300)
/label=OL2602
primer_bind complement(269..286)
/label=OL2596
primer_bind complement(269..286)
/label=OL2600
primer_bind complement(269..286)
/label=OL2606
primer_bind complement(269..286)
/label=OL2608
misc_feature 287..306
/label=SETBP1 spacer
primer_bind 297..342
/label=OL2603
misc_RNA 307..382
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
primer_bind complement(307..382)
/label=OL476
primer_bind 307..378
/label=OL2116
primer_bind 307..342
/label=OL2597
primer_bind 307..342
/label=OL2601
primer_bind 307..342
/label=OL2607
primer_bind 307..342
/label=OL2609
primer_bind complement(311..382)
/label=OL2117
primer_bind 311..348
/label=OL1453
primer_bind complement(354..373)
/label=OL1927
primer_bind complement(359..378)
/label=OL1940
primer_bind complement(365..406)
/label=OL2615
primer_bind complement(365..382)
/label=OL2619
primer_bind complement(365..382)
/label=OL2611
primer_bind complement(365..382)
/label=OL769
misc_feature 383..393
/label=RTT
primer_bind complement(388..443)
/label=OL2634
primer_bind complement(389..420)
/label=OL2747
primer_bind 394..438
/label=OL2763
misc_feature 394..406
/label=PBS
primer_bind 401..438
/label=OL2617
primer_bind complement(405..442)
/label=OL2618
misc_feature 407..414
/label=Linker
primer_bind complement(415..460)
/label=OL2621
primer_bind 415..456
/label=OL2620
misc_feature 415..451
/label=motif
primer_bind complement(415..449)
/label=OL2614
primer_bind complement(415..443)
/label=OL2635
primer_bind complement(415..441)
/label=OL2632
primer_bind 420..474
/label=OL2633
primer_bind 423..481
/label=OL2636
primer_bind complement(464..483)
/label=OL1916
primer_bind complement(478..494)
/label=OL1168
misc_feature 495..612
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
primer_bind complement(549..568)
/label=OL1936
primer_bind complement(555..579)
/label=OL1571
primer_bind complement(631..650)
/label=OL1088
promoter 661..1171
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
primer_bind complement(755..774)
/label=OL1068
primer_bind 903..925
/label=OL1709
primer_bind 945..961
/label=OL446
primer_bind 1090..1108
/label=hPGK-F
/note="Human PGK promoter, forward primer"
primer_bind complement(1173..1192)
/label=OL2256
primer_bind complement(1174..1195)
/label=OL1702
CDS 1193..1789
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(1193..1212)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 1196..1215
/label=OL2264
primer_bind 1689..1709
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
primer_bind 1689..1708
/label=OL2294
primer_bind 1689..1707
/label=OL2339
primer_bind 1760..1814
/label=OL901
primer_bind complement(1766..1789)
/label=OL306
primer_bind complement(1775..1797)
/label=OL384
primer_bind 1782..1813
/label=OL379
CDS 1790..1846
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
primer_bind 1790..1846
/label=OL866
primer_bind complement(1790..1846)
/label=OL867
primer_bind 1790..1816
/label=OL2251
primer_bind 1827..1870
/label=OL902
primer_bind complement(1827..1854)
/label=OL966
RBS 1829..1836
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
CDS 1847..2524
/codon_start=1
/product="monomeric derivative of DsRed (Campbell et al.,
2002)"
/label=mRFP1
/translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
TGA"
primer_bind 1847..1865
/label=OL2300
primer_bind 2078..2097
/label=OL1123
primer_bind complement(2087..2113)
/label=OL1728
primer_bind complement(2119..2145)
/label=OL2455
primer_bind complement(2502..2549)
/label=OL903
primer_bind complement(2502..2524)
/label=OL2353
primer_bind complement(2503..2524)
/label=OL2708
primer_bind 2523..2541
/label=OL965
primer_bind 2525..2547
/label=OL305
polyA_signal 2671..2792
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind 2671..2707
/label=OL1698
primer_bind complement(2682..2701)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 2736..2755
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(2776..2808)
/label=OL2209
primer_bind 2793..2826
/label=OL1692
primer_bind complement(2806..2832)
/label=OL1941
primer_bind complement(2981..3004)
/label=OL1043
primer_bind 2982..3004
/label=OL1044
primer_bind complement(3040..3057)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3211..3799)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3291..3310)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind complement(3393..3412)
/label=OL1041
primer_bind complement(3526..3545)
/label=OL631
primer_bind 3582..3601
/label=OL798
primer_bind complement(3823..3862)
/label=OL660
primer_bind 3843..3862
/label=OL659
CDS complement(3970..4830)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 4427..4446
/label=OL1922
primer_bind 4593..4612
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
primer_bind complement(4593..4612)
/label=OL1923
primer_bind 4746..4765
/label=OL1033
primer_bind complement(4746..4765)
/label=OL1034
promoter complement(4831..4935)
/gene="bla"
/label=AmpR promoter
rep_origin 4962..5417
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4987..4996)
/label=OL305
primer_bind complement(5049..5068)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5259..5280
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 5470..5489
/label=OL1469
primer_bind 5479..5495
/label=OL1109
polyA_signal 5548..5596
/note="synthetic polyadenylation signal"
misc_feature 5610..5701
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
primer_bind 5610..5626
/label=OL1167
primer_bind 5656..5675
/label=OL1087
primer_bind 5680..5694
/label=OL1935
primer_bind join(5710..5718,1..17)
/label=OL1700
ORIGIN
1 agtgaacgga tctcgacggt atcgatcacg agactagcct agagggggta tagctcagtg
61 gtagagcatt tgactgcaga tcaagaggtc cccggttcaa atccgggtgc cccctgagac
121 gatccccagc aacaggtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta
181 tcaacttgaa aaagtggcac cgagtcggtg cagagggggt atagctcagt ggtagagcat
241 ttgactgcag atcaagaggt ccccggttca aatccgggtg cccccttgtc ccaatgccgc
301 tgtcgcgttt tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga
361 aaaagtggca ccgagtcggt gcgatcccca gcaacagcgg cattggttaa atatcgcggt
421 tctatctagt tacgcgttaa accaactaga atttttttct cgacctcgag acaaatggca
481 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
541 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
601 attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcc gcggctcgag
661 ggggttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc
721 tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg actcgcacat tcttcacgtc
781 cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc
841 tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac
901 ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc
961 gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag
1021 cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct
1081 gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct
1141 cgttgaccga atcaccgacc tctctcccca gggggatcca ccggagctta ccatgaccga
1201 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
1261 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
1321 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
1381 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
1441 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
1501 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
1561 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
1621 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggaaacctc
1681 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
1741 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgccg ctactaactt
1801 cagcctgctg aagcaggctg gagacgtgga ggagaaccct ggacctatgg cgagtagcga
1861 agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca
1921 cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa
1981 actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt
2041 ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact
2101 gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt
2161 taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg
2221 tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc
2281 ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct
2341 gaaactgaaa gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa
2401 accggttcag ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa
2461 cgaagactac accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc
2521 ttaacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc
2581 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttctagagtc
2641 ggggcggccg gccgcttcga gcagacatga taagatacat tgatgagttt ggacaaacca
2701 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
2761 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
2821 ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
2881 gtaaaatcga taaggatccg tcgaccgatg cccttgagag ccttcaaccc agtcagctcc
2941 ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg
3001 caactcgtag gacaggtgcc ggcagcgctc ttccgcttcc tcgctcactg actcgctgcg
3061 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
3121 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
3181 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
3241 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
3301 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
3361 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
3421 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
3481 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
3541 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
3601 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
3661 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
3721 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
3781 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
3841 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
3901 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
3961 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
4021 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
4081 atctggcccc agtgctgcaa tgataccgcg ggacccacgc tcaccggctc cagatttatc
4141 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
4201 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
4261 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
4321 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
4381 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
4441 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
4501 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
4561 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
4621 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
4681 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
4741 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
4801 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
4861 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
4921 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc
4981 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
5041 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
5101 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
5161 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
5221 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
5281 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
5341 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
5401 attaacgctt acaatttgcc attcgccatt caggctgcgc aactgttggg aagggcgatc
5461 ggtgcgggcc tcttcgctat tacgccagcc caagctacca tgataagtaa gtaatattaa
5521 ggtacgggag gtacttggag cggccgcaat aaaatatctt tattttcatt acatctgtgt
5581 gttggttttt tgtgtgaatc gatagtacta acatacgctc tccatcaaaa caaaacgaaa
5641 caaaacaaac tagcaaaata ggctgtcccc agtgcaagtg caggtgccag aacatttctc
5701 tatcgatagg taccgatt
//
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