| 质粒名称: | pDG198 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002903 |
| 产品用途: | Mammalian PhCMV-driven constitutive expression vector for NS3/NS4A-tagged NanoLuc (PhCMV-NanoLuc-NS3/NS4A-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | NanoLuc, NS3/NS4A |
| 物种来源: |
synthetic
|
| 密码子优化: | 未优化 |
pDG198
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pDG198载体质粒基本信息
pDG198质粒图谱载体图谱和pDG198载体序列质粒序列多克隆位点信息
pDG198质粒载体简介
pDG198质粒序列载体序列
LOCUS pDG198 6884 bp DNA circular SYN 21-JUL-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6884)
AUTHORS Westlake University
TITLE Direct Submission
JOURNAL Exported Sep 12, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6884
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 826..845
/label=Primer 1
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 887..944
/label=oSCL1108
misc_feature 895..911
/label=MCS
/note="multiple cloning site"
primer_bind 897..944
/label=oSCL1314
primer_bind 898..936
/label=Primer 14
primer_bind 899..936
/label=Primer 7
primer_bind 899..936
/label=Primer 16
primer_bind 899..936
/label=Primer 19
primer_bind 899..936
/label=Fragment.REV
primer_bind 911..936
/label=Primer 1a
primer_bind 911..936
/label=Primer 9
promoter 911
/label=P_SV40
primer_bind 917..944
/label=oSCL1846
primer_bind 917..940
/label=oSCL1848
primer_bind 917..936
/label=Primer 3
primer_bind 921..944
/label=oSCL084
misc_feature 923..991
/label=Secretory (IgK)
/label=nonstandard type: signal
primer_bind complement(990..1009)
/label=Primer 8
primer_bind 1008..1029
/label=Primer 17
misc_feature 1013..1522
/label=NLuc (nano Luciferase)
/label=nonstandard type: reporter
primer_bind 1013..1032
/label=Primer 5
misc_feature 1043..1045
/label=cyt-STOP (TGG->TGA)
primer_bind complement(1502..1522)
/label=oSCL1849
primer_bind 1503..1560
/label=Primer 3a
primer_bind complement(1503..1527)
/label=Primer 15
primer_bind complement(1503..1522)
/label=Primer 4
primer_bind complement(1503..1522)
/label=Primer 6
primer_bind complement(1503..1522)
/label=Primer 10
primer_bind complement(1503..1522)
/label=Primer 18
primer_bind complement(1503..1522)
/label=Primer 20
primer_bind complement(1503..1522)
/label=Primer 13
primer_bind complement(1503..1522)
/label=Primer 22
primer_bind complement(1512..1522)
/label=oSCL085
primer_bind complement(1512..1522)
/label=oSCL1109
primer_bind 1534..1555
/label=Primer 12
primer_bind 1541..1599
/label=Primer 2a
CDS 1553..1576
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
primer_bind 1580..1638
/label=Primer 1`b
primer_bind 1598..1655
/label=SMASH-5
CDS 1613..2170
/codon_start=1
/label=NS3 protease
/translation="APITAYAQQTRGLLGCIITSLTGRDKNQVEGEVQIVSTATQTFLA
TCINGVCWAVYHGAGTRTIASPKGPVIQMYTNVDQDLVGWPAPQGSRSLTPCTCGSSDL
YLVTRHADVIPVRRRGDSRGSLLSPRPISYLKGSSGGPLLCPAGHAVGLFRAAVCTRGV
AKAVDFIPVENLETTMRSPVFTD"
primer_bind 1635..1693
/label=SMASH-6
primer_bind 1667..1725
/label=SMASH-7
primer_bind complement(1706..1764)
/label=SMASH-8
primer_bind complement(1745..1803)
/label=SMASH-9
primer_bind complement(1787..1845)
/label=SMASH-10
primer_bind complement(1823..1894)
/label=SMASH-11
primer_bind complement(1877..1935)
/label=SMASH-12
primer_bind complement(1918..1959)
/label=SMASH-13
primer_bind 1940..1995
/label=SMASH-14
primer_bind 1975..2033
/label=SMASH-15
primer_bind 2016..2074
/label=SMASH-16
primer_bind 2056..2114
/label=SMASH-17
primer_bind 2098..2156
/label=SMASH-18
primer_bind 2135..2193
/label=SMASH-19
CDS 2171..2203
/codon_start=1
/label=helicase domain
/translation="NSSPPAVTLTH"
primer_bind complement(2184..2242)
/label=Primer 4a
primer_bind complement(2184..2203)
/label=oSCL1389
primer_bind complement(2184..2203)
/label=oSCL1431
misc_feature 2199..2203
/label=Feature 48
primer_bind complement(2223..2281)
/label=Primer 5a
primer_bind complement(2262..2320)
/label=Primer 6a
primer_bind complement(2301..2359)
/label=Primer 7a
primer_bind complement(2340..2398)
/label=Primer 8a
primer_bind complement(2379..2427)
/label=Primer 9a
primer_bind complement(2385..2404)
/label=Primer 11
primer_bind complement(2405..2425)
/label=Primer 18
primer_bind complement(2405..2424)
/label=Primer 6
primer_bind 2406..2427
/label=Primer 21
misc_feature 2413..2458
/label=MCS
/note="multiple cloning site"
primer_bind complement(2458..2477)
/label=Primer 2
polyA_signal 2484..2708
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 2754..3182
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3196..3525
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3376..3511
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3511..3530
/label=Primer 1a
CDS 3592..4386
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin)"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 4560..4681
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(5132..5717)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5888..6748)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(6749..6853)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttgcca ccatgactag tgagacagac acactcctgc tatgggtact
961 gctgctctgg gttccaggtt ccactggtga cgctagtggt ggttctggta tggtcttcac
1021 actcgaagat ttcgttgggg actggcgaca gacagccggc tacaacctgg accaagtcct
1081 tgaacaggga ggtgtgtcca gtttgtttca gaatctcggg gtgtccgtaa ctccgatcca
1141 aaggattgtc ctgagcggtg aaaatgggct gaagatcgac atccatgtca tcatcccgta
1201 tgaaggtctg agcggcgacc aaatgggcca gatcgaaaaa atttttaagg tggtgtaccc
1261 tgtggatgat catcacttta aggtgatcct gcactatggc acactggtaa tcgacggggt
1321 tacgccgaac atgatcgact atttcggacg gccgtatgaa ggcatcgccg tgttcgacgg
1381 caaaaagatc actgtaacag ggaccctgtg gaacggcaac aaaattatcg acgagcgcct
1441 gatcaacccc gacggctccc tgctgttccg agtaaccatc aacggagtga ccggctggcg
1501 gctgtgcgaa cgcattctgg cgcccggggc cgggagttct ggtgacatca tggactacaa
1561 ggacgacgat gacaagggca gctcaggaac aggaagtggc tctggaacct cagcgcccat
1621 cacggcgtac gcccagcaga cgagaggcct cctagggtgt ataatcacca gcctgactgg
1681 ccgggacaaa aaccaagtgg agggtgaggt ccagatcgtg tcaactgcta cccaaacctt
1741 cctggcaacg tgcatcaatg gggtatgctg ggcagtctac cacggggccg gaacgaggac
1801 catcgcatca cccaagggtc ctgtcatcca gatgtatacc aatgtggacc aagaccttgt
1861 gggctggccc gctcctcaag gttcccgctc attgacaccc tgtacctgcg gctcctcgga
1921 cctttacctg gtcacgaggc acgccgatgt cattcccgtg cgccggcgag gtgatagcag
1981 gggtagcctg ctttcgcccc ggcccatttc ctacttgaaa ggctcctctg ggggtccgct
2041 gttgtgcccc gcgggacacg ccgtgggcct attcagggcc gcggtgtgca cccgtggagt
2101 ggctaaagcg gtggacttta tccctgtgga gaacctagag acaaccatga gatccccggt
2161 gttcacggac aactcctctc caccagcagt caccctgacg caccccatca ccaagatcga
2221 caccaagtac atcatgacct gcatgagcgc cgacctggag gtggtgacca gcacctgggt
2281 gctggtgggc ggcgtgctgg ccgccttggc tgcttattgc ctgagcaccg gctgcgtggt
2341 gatcgtgggc aggatcgtgc tgagcggcaa gcccgccatc atccccgaca gggaggtgct
2401 gtactgagaa ttctgcagat atccagcaca gtggcggccg ctcgagtcta gagggcccgt
2461 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
2521 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
2581 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
2641 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
2701 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
2761 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
2821 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
2881 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
2941 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
3001 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
3061 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
3121 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
3181 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
3241 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
3301 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
3361 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
3421 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
3481 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
3541 tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa
3601 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
3661 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
3721 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
3781 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
3841 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
3901 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
3961 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
4021 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
4081 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
4141 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
4201 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
4261 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
4321 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
4381 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
4441 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
4501 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
4561 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
4621 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
4681 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
4741 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
4801 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
4861 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
4921 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
4981 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
5041 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
5101 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
5161 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
5221 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
5281 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
5341 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
5401 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
5461 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
5521 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
5581 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
5641 ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg caagcagcag attacgcgca
5701 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
5761 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
5821 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
5881 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
5941 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
6001 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
6061 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
6121 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
6181 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
6241 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
6301 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
6361 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
6421 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
6481 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
6541 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
6601 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
6661 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
6721 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
6781 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
6841 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//
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