pGT145

LOCUS      pGT145            6779 bp DNA     circular SYN 21-JUL-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6779)
  AUTHORS   Westlake University
  TITLE     Direct Submission
  JOURNAL   Exported Sep 11, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6779
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    235..818
                     /label=P_CMV
                     /label=nonstandard type: promoter_const
     misc_feature    235..614
                     /label=P_CMV_enhancer
                     /label=nonstandard type: promoter_element
     misc_feature    615..818
                     /label=P_CMV_IE
                     /label=nonstandard type: promoter_element
     primer_bind     769..789
                     /label=oMHS-CMV-F
     misc_feature    829
                     /label=putative transcriptional start
     promoter        863..882
                     /label=T7 promoter
     primer_bind     887..935
                     /label=oSCL1092
     primer_bind     896..939
                     /label=OGT145-F
     primer_bind     917..940
                     /label=OGT132-1-F
     misc_feature    923..1828
                     /label=GLP1-Fc
     primer_bind     complement(1807..1861)
                     /label=OGT132-1-R
     primer_bind     complement(1807..1829)
                     /label=OGT143-1-R
     primer_bind     complement(1809..1828)
                     /label=oSCL1093
     primer_bind     complement(1810..1875)
                     /label=OGT145-R
     CDS             1829..1840
                     /codon_start=1
                     /label=FCS
                     /translation="RIKR"
     primer_bind     1836..1877
                     /label=olyl339
     CDS             1841..1855
                     /codon_start=1
                     /label=Linker
                     /translation="GSGGG"
     primer_bind     1853..1871
                     /label=OGT132-2-F
     misc_feature    1862..2340
                     /label=ecDHFR(N18T/A19V)
     primer_bind     complement(2334..2361)
                     /label=olyl340
     terminator      2376..2603
                     /label=bGH polyA
     rep_origin      2649..3077
                     /label=f1 bacteriophage origin of replication
                     /label=f1 bacteriophage origin of replication; arrow _f1 
                     ori
     misc_feature    3091..3420
                     /label=P_SV40
                     /label=nonstandard type: promoter_const
     rep_origin      3271..3406
                     /label=SV40 ORI
     misc_feature    3323..3372
                     /label=P_SV40 TSS-region
     CDS             3487..4281
                     /label=Translation 2136-2930
     misc_feature    3487..4278
                     /label=Kan/neoR
                     /label=nonstandard type: resistance
     primer_bind     complement(4621..4641)
                     /label=M13-rev
     misc_binding    complement(4647..4669)
                     /label=LacO
     promoter        complement(4673..4703)
                     /label=lac promoter
     rep_origin      complement(5027..5612)
                     /direction=LEFT
                     /label=high-copy-number ColE1/pMB1/pBR322/pUC origin o
                     /label=high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     _ori
     CDS             complement(5783..6643)
                     /label=Translation 5292-4432
     misc_feature    complement(5786..6643)
                     /label=AmpR
                     /label=nonstandard type: resistance
     promoter        complement(6644..6748)
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttgcca ccatgaagat catcctgtgg ctgtgtgtgt tcggcctgtt
      961 cctggccacc ctgttcccca tcagctggca gatgcccgtg gagtccggcc tgtcctccga
     1021 ggactccgcc agctccgaga gcttcgccaa gcgcatcaag cgccacggcg agggcacctt
     1081 caccagcgac gtgagcagct acctggaggg ccaggccgcc aaggagttca tcgcctggct
     1141 ggtgaagggc cgcggcagat ctggttgtaa gccttgcata tgtacagtcc cagaagtatc
     1201 atctgtcttc atcttccccc caaagcccaa ggatgtgctc accattactc tgactcctaa
     1261 ggtcacgtgt gttgtggtag acatcagcaa ggatgatccc gaggtccagt tcagctggtt
     1321 tgtagatgat gtggaggtgc acacagctca gacgcaaccc cgggaggagc agttcaacag
     1381 cactttccgc tcagtcagtg aacttcccat catgcaccag gactggctca atggcaagga
     1441 gttcaaatgc agggtcaaca gtgcagcttt ccctgccccc atcgagaaaa ccatctccaa
     1501 aaccaaaggc agaccgaagg ctccacaggt gtacaccatt ccacctccca aggagcagat
     1561 ggccaaggat aaagtcagtc tgacctgcat gataacagac ttcttccctg aagacattac
     1621 tgtggagtgg cagtggaatg ggcagccagc ggagaactac aagaacactc agcccatcat
     1681 ggacacagat ggctcttact tcgtctacag caagctcaat gtgcagaaga gcaactggga
     1741 ggcaggaaat actttcacct gctctgtgtt acatgagggc ctgcacaacc accatactga
     1801 gaagagcctc tcccactctc ctggtaaaag gatcaagagg ggttccggag gtggcgaatt
     1861 catgatcagc ctgatcgccg ccctggccgt ggaccacgtg atcggcatgg agaccgtgat
     1921 gccctggaac ctgcccgccg acctggcctg gttcaagaga aacaccctga acaagcccgt
     1981 gatcatgggc agacacacct gggagagcat cggcagaccc ctgcccggca gaaagaacat
     2041 catcctgagc agccagccca gcaccgacga cagagtgacc tgggtgaaga gcgtggacga
     2101 ggccatcgcc gcctgcggcg acgtgcccga gatcatggtg atcggcggcg gcagagtgta
     2161 cgagcagttc ctgcccaagg cccagaagct gtacctgacc cacatcgacg ccgaggtgga
     2221 gggcgacacc cacttccccg actacgagcc cgacgactgg gagagcgtgt tcagcgagtt
     2281 ccacgacgcc gacgcccaga acagccacag ctactgcttc gagatcctgg agagaagata
     2341 gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     2401 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     2461 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     2521 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     2581 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
     2641 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     2701 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     2761 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     2821 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     2881 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     2941 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     3001 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     3061 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     3121 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     3181 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     3241 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     3301 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct
     3361 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     3421 aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg
     3481 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     3541 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     3601 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     3661 gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     3721 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     3781 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     3841 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     3901 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     3961 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg
     4021 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     4081 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     4141 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     4201 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     4261 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc
     4321 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     4381 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     4441 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     4501 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     4561 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
     4621 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     4681 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     4741 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     4801 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     4861 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     4921 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     4981 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     5041 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     5101 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     5161 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     5221 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     5281 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     5341 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     5401 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     5461 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     5521 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggttttttt gtttgcaagc
     5581 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     5641 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     5701 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     5761 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     5821 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     5881 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     5941 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     6001 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     6061 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     6121 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     6181 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     6241 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     6301 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     6361 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     6421 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     6481 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     6541 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     6601 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     6661 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     6721 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtc
//