pCMV-mA3-BE3

LOCUS       pCMV-mA3-BE3                9132 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expresses mA3-BE3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-mA3-BE3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9132)
  AUTHORS   Wang X, Li J, Wang Y, Yang V, Wei J, Wu J, Wang R, Huang X, Chen J, 
            Yang L
  TITLE     Efficient base editing in methylated regions with a human 
            APOBEC3A-Cas9 fusion.
  JOURNAL   Nat Biotechnol.,  2018
REFERENCE   2  (bases 1 to 9132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9132
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(13..32)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        204..583
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        584..787
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     738..758
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     829..848
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        829..847
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      855..864
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             2196..6296
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6309..6557
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6570..6590
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6599..6616
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6639..6656)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6645..6869
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6940..6956)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6940..6956)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6953..6975)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6964..6980
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6988..7018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7033..7054
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7171..7188)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7342..7930)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7422..7441)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8101..8961)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8724..8743
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8962..9066)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc
       61 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
      121 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga
      181 tgtacgggcc agatatacgc gttgacattg attattgact agttattaat agtaatcaat
      241 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
      301 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
      361 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
      421 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
      481 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
      541 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
      601 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
      661 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
      721 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
      781 cagagctggt ttagtgaacc gtcagatccg ctagagatcc gcggccgcta atacgactca
      841 ctatagggag agccgccacc atgggaccat tctgtctggg atgcagccat cgcaaatgct
      901 attcaccgat cagaaacctg atatctcaag aaacattcaa gttccacttt aagaacctag
      961 gctatgccaa aggccggaaa gataccttct tgtgctatga agtgactaga aaggactgcg
     1021 attcacccgt ctcccttcac catggggtct ttaagaacaa ggacaacatc cacgctgaaa
     1081 tctgcttttt atactggttc catgacaaag tactgaaagt gctgtctccg agagaagagt
     1141 tcaagatcac ctggtatatg tcctggagcc cctgtttcga atgtgcagag cagatagtaa
     1201 ggttcctggc tacacaccac aacctgagcc tggacatctt cagctcccgc ctctacaacg
     1261 tacaggaccc agaaacccag cagaatcttt gcaggctggt tcaggaagga gcccaggtgg
     1321 ctgccatgga cctatacgaa tttaaaaagt gttggaagaa gtttgtggac aatggtggca
     1381 ggcgattcag gccttggaaa agactgctta caaattttag ataccaggat tctaagcttc
     1441 aggagattct gagaccttgc tacatctcgg tcccttccag ctcttcatcc actctgtcaa
     1501 atatctgtct aacaaaaggt ctcccagaga cgaggttctg ggtggagggc aggcgaatgg
     1561 acccgctaag tgaagaggaa ttttactcgc agttttacaa ccaacgagtc aagcatctct
     1621 gctactacca ccgcatgaag ccctatctat gctaccagct ggagcagttc aatggccaag
     1681 cgccactcaa aggctgcctg ctaagcgaga aaggcaaaca gcatgcagaa atcctcttcc
     1741 ttgataagat tcggtccatg gagctgagcc aagtgacaat cacctgctac ctcacctgga
     1801 gcccctgccc aaactgtgcc tggcaactgg cggcattcaa aagggatcgt ccagatctaa
     1861 ttctgcatat ctacacctcc cgcctgtatt tccactggaa gaggcccttc cagaaggggc
     1921 tgtgttctct gtggcaatca gggatcctgg tggacgtcat ggacctccca cagtttactg
     1981 actgctggac aaactttgtg aacccgaaaa ggccgttttg gccatggaaa ggattggaga
     2041 taatcagcag gcgcacacaa aggcggctcc gcaggatcaa ggagtcctgg ggtctgcaag
     2101 atttggtgaa tgactttgga aacctacagc ttggaccccc gatgtctagc ggcagcgaga
     2161 ctcccgggac ctcagagtcc gccacacccg aaagtgataa aaagtattct attggtttag
     2221 ccatcggcac taattccgtt ggatgggctg tcataaccga tgaatacaaa gtaccttcaa
     2281 agaaatttaa ggtgttgggg aacacagacc gtcattcgat taaaaagaat cttatcggtg
     2341 ccctcctatt cgatagtggc gaaacggcag aggcgactcg cctgaaacga accgctcgga
     2401 gaaggtatac acgtcgcaag aaccgaatat gttacttaca agaaattttt agcaatgaga
     2461 tggccaaagt tgacgattct ttctttcacc gtttggaaga gtccttcctt gtcgaagagg
     2521 acaagaaaca tgaacggcac cccatctttg gaaacatagt agatgaggtg gcatatcatg
     2581 aaaagtaccc aacgatttat cacctcagaa aaaagctagt tgactcaact gataaagcgg
     2641 acctgaggtt aatctacttg gctcttgccc atatgataaa gttccgtggg cactttctca
     2701 ttgagggtga tctaaatccg gacaactcgg atgtcgacaa actgttcatc cagttagtac
     2761 aaacctataa tcagttgttt gaagagaacc ctataaatgc aagtggcgtg gatgcgaagg
     2821 ctattcttag cgcccgcctc tctaaatccc gacggctaga aaacctgatc gcacaattac
     2881 ccggagagaa gaaaaatggg ttgttcggta accttatagc gctctcacta ggcctgacac
     2941 caaattttaa gtcgaacttc gacttagctg aagatgccaa attgcagctt agtaaggaca
     3001 cgtacgatga cgatctcgac aatctactgg cacaaattgg agatcagtat gcggacttat
     3061 ttttggctgc caaaaacctt agcgatgcaa tcctcctatc tgacatactg agagttaata
     3121 ctgagattac caaggcgccg ttatccgctt caatgatcaa aaggtacgat gaacatcacc
     3181 aagacttgac acttctcaag gccctagtcc gtcagcaact gcctgagaaa tataaggaaa
     3241 tattctttga tcagtcgaaa aacgggtacg caggttatat tgacggcgga gcgagtcaag
     3301 aggaattcta caagtttatc aaacccatat tagagaagat ggatgggacg gaagagttgc
     3361 ttgtaaaact caatcgcgaa gatctactgc gaaagcagcg gactttcgac aacggtagca
     3421 ttccacatca aatccactta ggcgaattgc atgctatact tagaaggcag gaggattttt
     3481 atccgttcct caaagacaat cgtgaaaaga ttgagaaaat cctaaccttt cgcatacctt
     3541 actatgtggg acccctggcc cgagggaact ctcggttcgc atggatgaca agaaagtccg
     3601 aagaaacgat tactccatgg aattttgagg aagttgtcga taaaggtgcg tcagctcaat
     3661 cgttcatcga gaggatgacc aactttgaca agaatttacc gaacgaaaaa gtattgccta
     3721 agcacagttt actttacgag tatttcacag tgtacaatga actcacgaaa gttaagtatg
     3781 tcactgaggg catgcgtaaa cccgcctttc taagcggaga acagaagaaa gcaatagtag
     3841 atctgttatt caagaccaac cgcaaagtga cagttaagca attgaaagag gactacttta
     3901 agaaaattga atgcttcgat tctgtcgaga tctccggggt agaagatcga tttaatgcgt
     3961 cacttggtac gtatcatgac ctcctaaaga taattaaaga taaggacttc ctggataacg
     4021 aagagaatga agatatctta gaagatatag tgttgactct taccctcttt gaagatcggg
     4081 aaatgattga ggaaagacta aaaacatacg ctcacctgtt cgacgataag gttatgaaac
     4141 agttaaagag gcgtcgctat acgggctggg gacgattgtc gcggaaactt atcaacggga
     4201 taagagacaa gcaaagtggt aaaactattc tcgattttct aaagagcgac ggcttcgcca
     4261 ataggaactt tatgcagctg atccatgatg actctttaac cttcaaagag gatatacaaa
     4321 aggcacaggt ttccggacaa ggggactcat tgcacgaaca tattgcgaat cttgctggtt
     4381 cgccagccat caaaaagggc atactccaga cagtcaaagt agtggatgag ctagttaagg
     4441 tcatgggacg tcacaaaccg gaaaacattg taatcgagat ggcacgcgaa aatcaaacga
     4501 ctcagaaggg gcaaaaaaac agtcgagagc ggatgaagag aatagaagag ggtattaaag
     4561 aactgggcag ccagatctta aaggagcatc ctgtggaaaa tacccaattg cagaacgaga
     4621 aactttacct ctattaccta caaaatggaa gggacatgta tgttgatcag gaactggaca
     4681 taaaccgttt atctgattac gacgtcgatc acattgtacc ccaatccttt ttgaaggacg
     4741 attcaatcga caataaagtg cttacacgct cggataagaa ccgagggaaa agtgacaatg
     4801 ttccaagcga ggaagtcgta aagaaaatga agaactattg gcggcagctc ctaaatgcga
     4861 aactgataac gcaaagaaag ttcgataact taactaaagc tgagaggggt ggcttgtctg
     4921 aacttgacaa ggccggattt attaaacgtc agctcgtgga aacccgccaa atcacaaagc
     4981 atgttgcaca gatactagat tcccgaatga atacgaaata cgacgagaac gataagctga
     5041 ttcgggaagt caaagtaatc actttaaagt caaaattggt gtcggacttc agaaaggatt
     5101 ttcaattcta taaagttagg gagataaata actaccacca tgcgcacgac gcttatctta
     5161 atgccgtcgt agggaccgca ctcattaaga aatacccgaa gctagaaagt gagtttgtgt
     5221 atggtgatta caaagtttat gacgtccgta agatgatcgc gaaaagcgaa caggagatag
     5281 gcaaggctac agccaaatac ttcttttatt ctaacattat gaatttcttt aagacggaaa
     5341 tcactctggc aaacggagag atacgcaaac gacctttaat tgaaaccaat ggggagacag
     5401 gtgaaatcgt atgggataag ggccgggact tcgcgacggt gagaaaagtt ttgtccatgc
     5461 cccaagtcaa catagtaaag aaaactgagg tgcagaccgg agggttttca aaggaatcga
     5521 ttcttccaaa aaggaatagt gataagctca tcgctcgtaa aaaggactgg gacccgaaaa
     5581 agtacggtgg cttcgatagc cctacagttg cctattctgt cctagtagtg gcaaaagttg
     5641 agaagggaaa atccaagaaa ctgaagtcag tcaaagaatt attggggata acgattatgg
     5701 agcgctcgtc ttttgaaaag aaccccatcg acttccttga ggcgaaaggt tacaaggaag
     5761 taaaaaagga tctcataatt aaactaccaa agtatagtct gtttgagtta gaaaatggcc
     5821 gaaaacggat gttggctagc gccggagagc ttcaaaaggg gaacgaactc gcactaccgt
     5881 ctaaatacgt gaatttcctg tatttagcgt cccattacga gaagttgaaa ggttcacctg
     5941 aagataacga acagaagcaa ctttttgttg agcagcacaa acattatctc gacgaaatca
     6001 tagagcaaat ttcggaattc agtaagagag tcatcctagc tgatgccaat ctggacaaag
     6061 tattaagcgc atacaacaag cacagggata aacccatacg tgagcaggcg gaaaatatta
     6121 tccatttgtt tactcttacc aacctcggcg ctccagccgc attcaagtat tttgacacaa
     6181 cgatagatcg caaacgatac acttctacca aggaggtgct agacgcgaca ctgattcacc
     6241 aatccatcac gggattatat gaaactcgga tagatttgtc acagcttggg ggtgactctg
     6301 gtggttctac taatctgtca gatattattg aaaaggagac cggtaagcaa ctggttatcc
     6361 aggaatccat cctcatgctc ccagaggagg tggaagaagt cattgggaac aagccggaaa
     6421 gcgatatact cgtgcacacc gcctacgacg agagcaccga cgagaatgtc atgcttctga
     6481 ctagcgacgc ccctgaatac aagccttggg ctctggtcat acaggatagc aacggtgaga
     6541 acaagattaa gatgctctct ggtggttctc ccaagaagaa gaggaaagtc taaccggtca
     6601 tcatcaccat caccattgag tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     6661 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     6721 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     6781 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     6841 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     6901 gataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     6961 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     7021 ctagggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     7081 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     7141 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     7201 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     7261 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     7321 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     7381 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     7441 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     7501 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     7561 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     7621 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     7681 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     7741 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     7801 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     7861 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     7921 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     7981 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     8041 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     8101 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     8161 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     8221 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     8281 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     8341 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     8401 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     8461 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     8521 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     8581 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     8641 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     8701 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     8761 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     8821 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     8881 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     8941 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     9001 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     9061 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcgatctcc
     9121 cgatccccta gg
//