Tol2-elavl3-H2B-GCaMP6s

LOCUS       Tol2-elavl3-H2B-GCaMP6s               14296 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expressed nuclear-localized GCaMP6-slow, a calcium indicator, in 
            neurons in zebrafish larva. The plasmid is in Tol2 transposon 
            backbone.
ACCESSION   .
VERSION     .
KEYWORDS    Tol2-elavl3-H2B-GCaMP6s
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14296)
  AUTHORS   Freeman J, Vladimirov N, Kawashima T, Mu Y, Sofroniew NJ, Bennett 
            DV, Rosen J, Yang CT, Looger LL, Ahrens MB
  TITLE     Mapping brain activity at scale with cluster computing.
  JOURNAL   Nat Methods. 2014 Jul 27. doi: 10.1038/nmeth.3041.
  PUBMED    25068736
REFERENCE   2  (bases 1 to 14296)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14296
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             9463..9840
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      9853..9862
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             9859..11211
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6s
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFHIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFGVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      9955..9964
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     primer_bind     10245..10266
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(10372..10393)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(10633..10652)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    11253..11387
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11277..11296)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     11331..11350
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(12059..12075)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12059..12075)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(12112..12132)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(12112..12130)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(12151..12167)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12151..12167)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12164..12186)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12175..12191
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(12199..12229)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12244..12265
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12382..12399)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12553..13141)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12633..12652)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(13312..14172)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13935..13954
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14173..14277)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 atgctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttcccg
      721 ggtgtcgacg aattctcgag ctctattcct aaagaccttg ggtgaccaaa atcttatttt
      781 aataaataaa actgtttatt aaaacttttt tgtttcaaag aaccatatgt atagtgaaat
      841 ttataaaaat atcaattttt aaaaagctgg tgtactcatt tatgttatga actctaaaac
      901 catatactga ctgcaagtga tgatgtatag agtgatgttt acgagtaaac atatttagtt
      961 gtatacatcc tactgagcac attttgatgt atgaaataac attacaagct ttatccaaat
     1021 taagccattt taaaacactg ccaattgaaa atacaaatcc tggaaaaaat cgtctttagc
     1081 gcagtcattt gagccatcct aatccgttac ctcagaccat aataagaagg gataacacta
     1141 gctgtagcaa tggaacacat ctgtttcaca caatcatatc tcctgcgccg gtgctaagca
     1201 gattcagcgt gatcataaca tgctttccac tcataaatgt aaatttacaa tttgcacatg
     1261 taaaacagac acttttgaga tattggataa aaaaacaaga gtatattgct tagtttcatc
     1321 caccagtcat ccccacagcg tttggaaggc cataaaaagt gtctaaaatc aatgatcatt
     1381 gaaagagcac aagagagact cttacgctgt aatgccactg gggacaaaag tgacagtctc
     1441 ttaatgggct cttctggagg ggctcctgaa cattaaaaat tatcagcgaa attaccgaaa
     1501 gagcttcaag caactggcat gcttgatcct ctgcgtcggg gcggtgaata ggtgcttcag
     1561 atgccctctt acccacgggc tggattcagc tgccccgcta ccagcggaga ccccctaatg
     1621 agcctctgca attaagttta ttcatgttaa gtgtgaacgg ggtgcgtgcg gaactgtggg
     1681 cagctaacag acctgggttc tttgtgccac aagtgctgcc tttattcggc tcacaaagca
     1741 gaaaacaaca cccgcaccta taatggcgcc ctcggctggg tctaagaaac gtggcgagtt
     1801 gacagagcag agtgggcggg gttaagacag actgacagcg ggacccatct ccatcctctt
     1861 attaacgctt aacgagtgcc ttcctcatgc aatattcatc gccactaata tcatccaagc
     1921 tctgagctga gctggccact tatgtaaggc aattatgtaa aatatcagac agggcccaca
     1981 ctcagaatct gactggggta gagacgcggg acgagaaccg agagcaagaa ctgaaagtga
     2041 aagtgaccac taaagggagg agaggacaga ggggcaggat gtgtcaagat taccagagaa
     2101 cacttggcca gaaatgcgca accattggag ctctccggat tacccaaagg ttaacgagtt
     2161 tgaacgcctc tgcccactcg cccatctctg atggtttccc aagaactcct caagcaaaat
     2221 atatataatt gtgtgtatta tgcacagaca cgagaaaatg ctgtttttct gatctgcatt
     2281 acagcacatt tgcccgccaa cgacaatacc acccactcgg tacctcgctg actcctgatg
     2341 cctgatacct gcgcggtgac tgtctacaat ctgcataatc aagagaagtt gtgttgaaga
     2401 cgagcgccac acaaccgttt ccacaaggtc acccaaggcc ggtgcagatg taggtgaggt
     2461 ctccataaac agactgaaat aaacacatcc tccgctggga acaacaaccc cctcacgcct
     2521 catgcatttc cataagccta catgcatctc ttccaactta tggagactcg cacctaccaa
     2581 catccgcaca acaaagatat acagagcgcg ctccctcagg tcaaggccct gtgggggtct
     2641 gtgcagaaat aggtcatttg tcacacatca agtcctgggg caggagatgc attatagatg
     2701 agaccaaaca gcctgtctcg gtgagctcta cccactccct gagactagaa atgggggaag
     2761 ggagcttgag ataacaaccg ctgcaatcac tgtgtcgatg tttaatatca gcaccaaccg
     2821 ggaacaataa ggagatgggt gcattcatgt tcacatctta ccagtcaagt atcatcgaac
     2881 cggcttgata accacacctc gtgtaatagc tgagcagata gttgtcattt taaagcgttg
     2941 gcctttgtcg attatgtaat gcgcacattc aacacatggt aatatagaaa cggttatgtc
     3001 gaggttgttt tgtccagaga tgaccttcac acagttacag ccgctctgca tccacacaaa
     3061 tggaggactt aatcgtggac tgcattctta gaaatgatct acaaagacaa ataatgtgaa
     3121 atcaagaaag gacaaaattt aagtaagggg atgagggaga gagagaacga ggggcaagga
     3181 gaaagcatgg ctcctgtctt tttctgcacc catctgttcg gagtgcaggt ggagctctat
     3241 tcactcagct ctgcatgtgt gtttgggggg ggcaggaaga aagggagggc aaaaggaaga
     3301 gtggagagat ggtgggggct ggagggatgg ggggttctcg gtgatctctc ctgaagggga
     3361 taatgggaga gcagcgcttt gcaatggctg ccatgtagta ccctccctgc acaattagcc
     3421 aatcagcagc aagctctgcc agccagaagg acacataaaa gaagaacatt gcagcagagg
     3481 cacagaagga gcctgcgagg agctgggaaa tacacacaca acagcagaac cacaacaccc
     3541 tcccctggac acaccctact ggggatcact gcttttcttt ttttctgaac catcgcccac
     3601 gccacacgga gagaaatctc tctctcatca tcatcctgaa gaaaaccccc ttatcctcat
     3661 tttcacactg ctgaggaaaa cctacaatcg cacgggctga gatttcctgg cgaagactgt
     3721 cctttttcct ttttcttttt ttttcttttc ctttggaaac tgacatttgc atttctccat
     3781 tccaagccac ggcgtaataa tatctgcaat ccagcctgaa gacctgcaaa tcgaaggacc
     3841 tagatcacta tcatctttgt acgtcaagga tcctggttac tgtacgtata acctttcttt
     3901 cttttgctct gaccaatatg aaacactaaa atcgattcga gcagcctctc agcatcaatt
     3961 acagtgcgtg aaaaacattc aaatagaggc agcaaatatt acatgtgaaa atacaggctg
     4021 gctaaatcca agctaattta gaaatgtggt caaaacgcat actggcacgt ctaatcgcgg
     4081 attcagtaaa caagattaac gattagccca gtgtataagt catatgatac cggcatgcgc
     4141 gagagcatcg ctacacccga gctggcttca ttttcggagg aaaatcaaaa cattgctttc
     4201 tcctgccgtg cgaaccattc gtcataaacc gtaatacgca acatacattt attactacat
     4261 ccgttaatta gcgataatta gccgttatta acaaagagcg ctgaggaatt cctctcaaat
     4321 agcggaggtg cggcggaggc agaggggcgt aaaagggcac atgcgtgcct ggctcaaaaa
     4381 aggatgctcc agactgaaac tcagagacaa aacacgatgc ctcggaggct gagagcccat
     4441 cgagaggaaa ggcaaagaaa agggtcgagt cactcagagg gggaggggag atattgtgca
     4501 tccattggtt tgaattgaag caggcagaat aaaaatcctt gcgattatgc ttcgtgagag
     4561 atcgcgagag aggaaaaggc taaatgccac gtatgcaaat ggataaatgt catctatttc
     4621 ttcgcgagta gacattttgt ggacaacgag agtgtaaata agcgatatga ggggaataca
     4681 aaggccttga gggaaagcgt tttgggcgtg tcttgtcgaa aagaagacag cgatggcacg
     4741 cgctcagaac caccatcttc acgtagacaa tccgcgtgaa aacaataaca aaactgctca
     4801 ttttaacacg agcaggtgtt tgaaaccacc caaaaaagat tgtctaaaat attaagatta
     4861 attaatgacg tttaaatgtc caggcctgat ataaatgaag cgtgtagtaa gagtaatcgc
     4921 ctttgtttct tttcagagat acgttttcag agattcaggg aattatatct tcaataaaat
     4981 aatgtttgat ttcattattt taaagctttt taagaagtaa aaatacacat tattcattta
     5041 cattttattt tatttaaagg cctatggtct tgaaaataat aagtggaata tataggctat
     5101 atattcgtct aaggacagcc ggtgttattt tatagtgacc tcagacaaaa gccgagacaa
     5161 aacgaggcgc ttgcctttca ttttaatcct taatagacgg tgtgatgaat gaatgaatga
     5221 atgaataaat gaatgaatga cgggcccttc ggcatgttgt tccgcagtcc tccgtgagca
     5281 cgagtctcta ccgcagtgcc aagcacataa aaccgttgag tcagataacc ttctccaggc
     5341 gcgtatacac aattcaagga caagcacatc acaactatac acaacacaac acaaccgcct
     5401 aaacgcacga gctcatcaat cgcaatttaa aggcatgtca gtcaaagaaa aaggcggcgt
     5461 tatttaaaac caatgttcaa caatatttgt atgctgacta gcccgggcgc gcacgctata
     5521 agatcacaag gccttgtttt ttccctttat tcaaaatatc attaattatt acaggtcata
     5581 aaacaaaaca atgcgaatta caatgattta aagactattg atttaacaat aatcagttaa
     5641 ttatagaata atattataat attttacata caaatgaaag atgacattat ttgatgacat
     5701 tgtaattaaa atatatgcaa tatattttta cgtttttatt aattaggcta ttactattat
     5761 tataattatc attattttta ttattattat tattattatt aaatagtctt gcgaaataag
     5821 agtgaaggca gttgcaatgt tcattgttcc cggagagtcc cgcagcctct ggcctcaaca
     5881 caaagcgcta ctgtttacat taatattcat agtgctgcca tggtctctgt agaggaggaa
     5941 cgggtggctc ttttgttcgc tagtgtcaat acgtttattc agcaaaaagc agctcggatt
     6001 tgtgaatgcc caacaagcac acgcgcactc caatcaaagg acgagaggat aacgatttta
     6061 aacgattcgc aaacgtgttt atttgtaaag acgacatcaa gagcttaatg tccacttgaa
     6121 aagaaataaa tatgcccgcg gcgccaagga tattttgaat gtagtcgttc atggtgagcc
     6181 actcccgaat tcagcactct ggagagcgac caaagaggaa tagcggaata gcaactactt
     6241 gagccatttc tctccgtttg atggcgttca tgattaaaaa tataatcacg tgatttttta
     6301 tacatatata aatatataca taaagtaacg ctttcctttt gagaatacat aaatatttat
     6361 tcgcgtgata aatcaatgat cttatattta tttgcacgac cgaaattagc caaattcacc
     6421 aattaaaaaa aaaataagcc aacaaaaaag aagggtttat ccgttcaatt ttgacttgtg
     6481 tcctgttgtt ttacagctgc cactgtgatc ccttaagctg cagtgagagt gtggctaatg
     6541 ccttttgttt aagataatcc tgtaatctgt taccgaaacg gcctattgac aagccggcat
     6601 tcacatttca gtcaggaggc ccgtccagac atgtacattt atgaattacg aatgataaaa
     6661 ttaagatctg cattaggcgt cagaaatgtc acgaacaccc attcatttcc cacagccaat
     6721 aatggatcat gctgggaagc gctatcctcg cagtctcaaa attaatgttg acatgttgcg
     6781 tagagctaca gattagatca gccatatgct ttatgtgttt acctcagcgg atcttcagca
     6841 agatgtgttt attttaagaa aatggcttcc tcgctgcctg cacaggggca ttaaggaagt
     6901 gacgtgagcg tcctcacagt caaattactt tatctcatgc tgctttcagg cctctgctta
     6961 ttttattatt attattttaa attaggggga atcacggtgg cgttgtgggt agggtagcgc
     7021 gatcacctta cagcaagaag gtcgctggtt cgagctctgg ctgggtcagt tggcatttct
     7081 gtgtggagtt taaattgaat tgaaattgaa taaactaaat tggccctagt gtatgtgtgt
     7141 gaatgtaagt gtgcatgggt gtttcccagt gttgggtttg actggaagag catccgcttc
     7201 gtaaaacata tgctggataa gttggcggtt catttcactg tggtgaccgc tgattaataa
     7261 agggactaag ctgaaaatga aatgaattaa tacattttaa atgagctttt gctgacatgt
     7321 ttatgattac aaatagtgaa attgtatgtt aatttacgat aagtgcattg ttctaaattc
     7381 atttttatgt agcagctggt ttttcttcca tttgtgtcat tcaacagaac atttttctaa
     7441 aaaagtgaac ttaaattttc tcctccctct gacaaggctt gatggtaatt tcagccgact
     7501 ctaattacag actcactaaa aggaatcggt gcagtcattt atctttatca gcgcgctggt
     7561 gggaaggtaa taggttttgc ttatgagttg tttgcgtgcg agtctgggtc cttgtgtagg
     7621 cgatgctgat gcactgcatt aaatattgag ggagaagtcg ctcaaaaata gcaaagtaca
     7681 ggctggggca ggaacgtcaa agcccgctca gtggccacgt ttcttgtttc gtaagtccta
     7741 tttttaatag cgataattca ggctcagtag aaggaactcc agcactataa gcagtctctc
     7801 gcctgtctcg gcgagttatg tattttggga tgaagtgctc ttccacctct acctctgctg
     7861 tgctggcgcc agtatctggg ctgtaagata gtgtacgtgt cagtctgtgt ggccgaagag
     7921 cctgtgtgtt gtgtaactca ctacattcgt cgctgtggat ggctgacttg ccatggggag
     7981 gtttacttca gttcacgaat cacattcgcc acgatcttgt cactttgtgt caagactcgc
     8041 tctctgtgtg tcaacagctc tgctgtttga ggttgaaatt ctgaacttga gatatatccc
     8101 aggatatatt agggctactg ccaacggaaa aagcaaatgg tgccatggcc atttgattga
     8161 tcaaacaaac acccttttgc ttgggtgaca gtgaaatgtc agatccatgt ttgtactgct
     8221 ttaataactt gcctctctga gctttctcct tgtcagtgtc aatttgcctg agatataaat
     8281 tccggttgcc ccgaaagctt attttattaa aaaaaagcta acatgcactc atacaacgtg
     8341 cgcacataca tgtgaggctt gctaaaagtc aagaagcaag ccaatagcaa gagtcgtcag
     8401 actgctgtta cctgggcaac ctggttagag gtaaggctca aggcaagaat aaatcattcc
     8461 actgaagaga cacagcgtta cagcgagatg agagcaagag gcgatagtga agggagaaca
     8521 aaagaacagc ctacccacac tagtgcagat ctacctgttt gagctactga ttgttcagtt
     8581 tgattagata tcagacaatt ctgctatctc caattgcatt gctgcatgaa ttcctagaaa
     8641 tcattttaat caccaaaaac tagtagggac acttgtggcg tatatgattc tgcctgttga
     8701 ttgtggggat ccaacagaaa gatcatgaat gcattgttaa ttaagtcggt gagacaccct
     8761 gttccaccct aggggccgtt ctggggaaag agtgctttca gtcagctaaa gatgactaat
     8821 atgtgaacat atattactac acatgccaat ttgtacattc tggatagtta ccagccctgg
     8881 gaaaacatcg aaaaaaataa atcaacatag ataaaattgg caaatccctt ctggtacgat
     8941 gcagagtgct tgctctgaga acttgtgcat tgtgacagaa agcgaccgtt aaaaactgaa
     9001 agtagcatct acaacctgca tacatctgtt ctttgaaaat cccctgtagg ccaaattaag
     9061 catcacgctg tgctccacag caacacggat gaaccgaaaa cccagacaca aacgcacaca
     9121 cttttcacat aaacaaggtt tagttatgct agctatgctt tttcgtcctc cctttgtcat
     9181 cagcggcgag tgacgtaaca cagtttgacc ctggacagcg taaagggggg gttgggaatg
     9241 agtgaaagga ctgtgacatc tgcagggcca ggggctgatg gagagagctg aagtgaggga
     9301 ggtcagagga ggaggaaggg tgggatattg tcctcctcag gccctatccc tctcagacgc
     9361 attgattttt aacctctcag cgagaatgcc aagcattaca cccctcaaat tatggttcct
     9421 cacccaacct gttatatttt ccacctgcag ataattaccg gtatgccaga gccagcgaag
     9481 tctgctcccg ccccgaaaaa gggctccaag aaggcggtga ctaaggcgca gaagaaaggc
     9541 ggcaagaagc gcaagcgcag ccgcaaggag agctattcca tctatgtgta caaggttctg
     9601 aagcaggtcc accctgacac cggcatttcg tccaaggcca tgggcatcat gaattcgttt
     9661 gtgaacgaca ttttcgagcg catcgcaggt gaggcttccc gcctggcgca ttacaacaag
     9721 cgctcgacca tcacctccag ggagatccag acggccgtgc gcctgctgct gcctggggag
     9781 ttggccaagc acgccgtgtc cgagggtact aaggccatca ccaagtacac cagcgctaag
     9841 gatccaccag tcgccaccat gggttctcat catcatcatc atcatggtat ggctagcatg
     9901 actggtggac agcaaatggg tcgggatctg tacgacgatg acgataagga tctcgccacc
     9961 atggtcgact catcacgtcg taagtggaat aagacaggtc acgcagtcag agctataggt
    10021 cggctgagct cactcgagaa cgtctatatc aaggccgaca agcagaagaa cggcatcaag
    10081 gcgaacttcc acatccgcca caacatcgag gacggcggcg tgcagctcgc ctaccactac
    10141 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
    10201 gtgcagtcca aactttcgaa agaccccaac gagaagcgcg atcacatggt cctgctggag
    10261 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaggg cggtaccgga
    10321 gggagcatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
    10381 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggtga gggcgatgcc
    10441 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
    10501 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
    10561 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacatcca ggagcgcacc
    10621 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
    10681 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
    10741 gggcacaagc tggagtacaa cctgccggac caactgactg aagagcagat cgcagaattt
    10801 aaagaggctt tctccctatt tgacaaggac ggggatggga caataacaac caaggagctg
    10861 gggacggtga tgcggtctct ggggcagaac cccacagaag cagagctgca ggacatgatc
    10921 aatgaagtag atgccgacgg tgacggcaca atcgacttcc ctgagttcct gacaatgatg
    10981 gcaagaaaaa tgaaatacag ggacacggaa gaagaaatta gagaagcgtt cggtgtgttt
    11041 gataaggatg gcaatggcta catcagtgca gcagagcttc gccacgtgat gacaaacctt
    11101 ggagagaagt taacagatga agaggttgat gaaatgatca gggaagcaga catcgatggg
    11161 gatggtcagg taaactacga agagtttgta caaatgatga cagcgaagtg accggtggat
    11221 cctggccgtc gttttactgt accatcgatg atgatccaga catgataaga tacattgatg
    11281 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
    11341 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
    11401 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
    11461 acctctacaa atgtggtatg gctgattatg atcctctaga tcagatctgc gaagatacgg
    11521 ccacgggtgc tcttgatcct gtggctgatt ttggactgtg ctgctcgcag ctgctgatga
    11581 atcacatact tcctccattt tcttccactg attgactgtt ataatttccc taatttccag
    11641 gtcaaggtgc tgtgcattgt ggtaatagat gtgacatgac gtcacttcca aaggaccaat
    11701 gaacatgtct gaccaatttc atataatgtg aaaacgattt tcataggcag aataaataac
    11761 atttaaatta aactgggcat cagcgcaatt caattggttt ggtaatagca agggaaaata
    11821 gaatgaagtg atctccaaaa aataagtact ttttgactgt aaataaaatt gtaaggagta
    11881 aaaagtactt ttttttctaa aaaaatgtaa ttaagtaaaa gtaaaagtat tgatttttaa
    11941 ttgtactcaa gtaaagtaaa aatccccaaa aataatactt aagtacagta atcaagtaaa
    12001 attactcaag tactttacac ctctggttct tgacccccta ccttcagcaa gcccagcaga
    12061 tccactagtt ctagagcggc cgccaccgcg gtggagctcc agcttttgtt ccctttagtg
    12121 agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
    12181 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
    12241 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
    12301 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
    12361 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
    12421 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
    12481 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
    12541 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
    12601 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
    12661 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
    12721 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
    12781 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
    12841 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
    12901 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
    12961 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
    13021 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
    13081 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
    13141 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
    13201 aggcattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
    13261 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
    13321 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
    13381 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
    13441 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
    13501 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
    13561 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
    13621 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
    13681 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
    13741 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
    13801 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    13861 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    13921 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    13981 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    14041 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    14101 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    14161 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    14221 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    14281 atttccccga aaagtg
//