| 质粒名称: | pYK73 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000789 |
| 产品用途: | Mammalian PhCMV-driven constitutive SEAP expression vector containing 4 tandem MS2-box repeats in the 3’UTR (PhCMV-SEAP-(MS2-box)4-pA). |
| 存储实验室: | Anping ZENG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: |
Stbl3
|
|
插入基因信息 |
|
| 插入基因名称: |
SEAP-(MS2-box)4
|
| 物种来源: | synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Programmable solid-state condensates for spatiotemporal control of mammalian gene expression
Wang Y., Jiang J., Xiong Q., Li S., Shao J., Xie M., Zeng A.P.
Nat. Chem. Biol. 2025 1–10 (2025)
|
pYK73
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pYK73载体质粒基本信息
pYK73质粒图谱载体图谱和pYK73载体序列质粒序列多克隆位点信息
pYK73质粒载体简介
pYK73质粒序列载体序列
LOCUS pYK73 7226 bp DNA circular SYN 11-FEB-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7226)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 24, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7226
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 232..819
/label=CMV
primer_bind 863..882
/label=T7
primer_bind 881..917
/label=oYK55-SEAP-F
primer_bind 881..900
/label=oYK06-ANR-mEGFP-F
primer_bind 900..927
/label=oDJ273-1
misc_feature 901..2460
/label=SEAP
primer_bind complement(2439..2460)
/label=oDJ273-2
primer_bind complement(2441..2475)
/label=pYK62-SEAP-R
primer_bind complement(2444..2460)
/label=Oscl31-4
primer_bind complement(2444..2460)
/label=oSCL083
polyA_signal 2452..2460
/label=SV40 late polyA
misc_feature 2469..2485
/label=oYK58-ms2-F
misc_feature 2469..2473
/label=New Feature
misc_feature 2469..2473
/label=New Feature
misc_feature 2492..2495
/label=linker
primer_bind complement(2497..2511)
/label=Oscl28-2
primer_bind complement(2498..2511)
/label=8x R
misc_feature 2512..2524
/label=linker
misc_feature 2530..2548
/label=MS2 box
misc_feature 2554..2563
/label=linker
misc_feature 2569..2587
/label=MS2 box
primer_bind complement(2581..2605)
/label=oYK55-SEAP-R
misc_feature 2593..2602
/label=linker
misc_feature 2593..2602
/label=linker
misc_feature 2601..2631
/label=hairpin
misc_feature 2607..2625
/label=MS2 box
misc_feature 2630..2642
/label=linker
misc_feature 2641..2671
/label=hairpin
misc_feature 2647..2665
/label=MS2 box
misc_feature 2675..2680
/label=linker
misc_feature 2689..2704
/label=linker
primer_bind complement(2703..2743)
/label=oYK73_msbox4_R
primer_bind complement(2724..2743)
/label=oYK55-SEAP-R
terminator 2820..3047
/label=bGH PA
misc_feature 3093..3521
/label=f1 origin
misc_feature 3526..3869
/label=SV40 early promoter and origin
misc_feature 3715..3792
/label=SV40 ORI
CDS 3934..4725
/label=Kan/neoR
misc_feature 4899..5029
/label=SV40 early polyadenylation signal
primer_bind complement(5065..5085)
/label=M13-rev
misc_binding complement(5091..5113)
/label=LacO
promoter complement(5118..5147)
/label=lac
misc_feature complement(5412..6085)
/label=pUC origin
CDS complement(6233..6892)
/label=AmpR
promoter complement(7132..7160)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 atgctgctgc tgctgctgct gctgggcctg aggctacagc tctccctggg catcatccca
961 gttgaggagg agaacccgga cttctggaac cgcgaggcag ccgaggccct gggtgccgcc
1021 aagaagctgc agcctgcaca gacagccgcc aagaacctca tcatcttcct gggcgatggg
1081 atgggggtgt ctacggtgac agctgccagg atcctaaaag ggcagaagaa ggacaaactg
1141 gggcctgaga tacccctggc catggaccgc ttcccatatg tggctctgtc caagacatac
1201 aatgtagaca aacatgtgcc agacagtgga gccacagcca cggcctacct gtgcggggtc
1261 aagggcaact tccagaccat tggcttgagt gcagccgccc gctttaacca gtgcaacacg
1321 acacgcggca acgaggtcat ctccgtgatg aatcgggcca agaaagcagg gaagtcagtg
1381 ggagtggtaa ccaccacacg agtgcagcac gcctcgccag ccggcaccta cgcccacacg
1441 gtgaaccgca actggtactc ggacgccgac gtgcctgcct cggcccgcca ggaggggtgc
1501 caggacatcg ctacgcagct catctccaac atggacattg acgtgatcct aggtggaggc
1561 cgaaagtaca tgtttcgcat gggaacccca gaccctgagt acccagatga ctacagccaa
1621 ggtgggacca ggctggacgg gaagaatctg gtgcaggaat ggctggcgaa gcgccagggt
1681 gcccggtatg tgtggaaccg cactgagctc atgcaggctt ccctggaccc gtctgtgacc
1741 catctcatgg gtctctttga gcctggagac atgaaatacg agatccaccg agactccaca
1801 ctggacccct ccctgatgga gatgacagag gctgccctgc gcctgctgag caggaacccc
1861 cgcggcttct tcctcttcgt ggagggtggt cgcatcgacc atggtcatca tgaaagcagg
1921 gcttaccggg cactgactga gacgatcatg ttcgacgacg ccattgagag ggcgggccag
1981 ctcaccagcg aggaggacac gctgagcctc gtcactgccg accactccca cgtcttctcc
2041 ttcggaggct accccctgcg agggagctcc atcttcgggc tggcccctgg caaggcccgg
2101 gacaggaagg cctacacggt cctcctatac ggaaacggtc caggctatgt gctcaaggac
2161 ggcgcccggc cggatgttac cgagagcgag agcgggagcc ccgagtatcg gcagcagtca
2221 gcagtgcccc tggacgaaga gacccacgca ggcgaggacg tggcggtgtt cgcgcgcggc
2281 ccgcaggcgc acctggttca cggcgtgcag gagcagacct tcatagcgca cgtcatggcc
2341 ttcgccgcct gcctggagcc ctacaccgcc tgcgacctgg cgccccccgc cggcaccacc
2401 gacgccgcgc acccgggtta ctctagagtc ggggcggccg gccgcttcga gcagacatga
2461 aaaccccaag cttggtaccg agctcggatc caacttgaac cggtaagaat tgcaacaaac
2521 aaatcaccac cgtgaggatc acccacggtg gtgaaagcct tagcctagcc gtgaggatca
2581 cccacggtag gcacaagaaa ttctacccgt gaggatcacc cacgggtaga atcacaaaga
2641 ctacagccgt gaggatcacc cacggctgta gactcaaact taaaaaggat atcttcagca
2701 aactgatcgg agacgagtcc gtcggatcca ctagtccagt gtggtggaat tctgcagata
2761 tccagcacag tggcggccgc tcgagtctag agggcccgtt taaacccgct gatcagcctc
2821 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
2881 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
2941 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
3001 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
3061 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
3121 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
3181 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
3241 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
3301 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
3361 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
3421 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
3481 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
3541 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
3601 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
3661 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
3721 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
3781 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
3841 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
3901 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
3961 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
4021 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
4081 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
4141 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
4201 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
4261 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
4321 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
4381 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
4441 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
4501 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
4561 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
4621 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
4681 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
4741 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
4801 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
4861 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
4921 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
4981 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
5041 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
5101 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
5161 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
5221 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
5281 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
5341 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
5401 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
5461 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
5521 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
5581 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
5641 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
5701 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
5761 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
5821 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
5881 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
5941 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
6001 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
6061 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
6121 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
6181 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
6241 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
6301 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
6361 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
6421 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
6481 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
6541 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
6601 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
6661 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
6721 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
6781 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
6841 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
6901 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
6961 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
7021 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
7081 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
7141 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
7201 ttccccgaaa agtgccacct gacgtc
//
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