| 出品公司: | |
|---|---|
| 载体名称: | phRL-TK |
| 质粒类型: | 哺乳动物表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | HSV-TK |
| 载体大小: | 4045 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
| 备注: | |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
phRL-TK载体质粒基本信息
phRL-TK质粒图谱载体图谱和phRL-TK载体序列质粒序列多克隆位点信息
phRL-TK质粒载体简介
phRL-TK质粒序列载体序列
LOCUS phRL-TK 4045 bp DNA circular SYN 10-JUL-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4045)
AUTHORS Biofeng
TITLE Direct Submission
JOURNAL Exported Jul 10, 2023 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4045
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 8..759
/label=HSV TK promoter
/note="herpes simplex virus thymidine kinase promoter"
intron 829..961
/label=chimeric intron
/note="chimera between introns from human beta-globin and
immunoglobulin heavy chain genes"
promoter 1006..1024
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
regulatory 1028..1037
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 1034..1969
/codon_start=1
/product="Renilla luciferase"
/label=hRluc
/note="human codon-optimized"
/translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
polyA_signal 2000..2121
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
promoter 2254..2358
/gene="bla"
/label=AmpR promoter
CDS 2359..3219
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 3390..3978
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 agatctaaat gagtcttcgg acctcgcggg ggccgcttaa gcggtggtta gggtttgtct
61 gacgcggggg gagggggaag gaacgaaaca ctctcattcg gaggcggctc ggggtttggt
121 cttggtggcc acgggcacgc agaagagcgc cgcgatcctc ttaagcaccc ccccgccctc
181 cgtggaggcg ggggtttggt cggcgggtgg taactggcgg gccgctgact cgggcgggtc
241 gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac ccccgggcgg cgccgccgcg
301 gcggcgacgg gctcgctggg tcctaggctc catggggacc gtatacgtgg acaggctctg
361 gagcatccgc acgactgcgg tgatattacc ggagaccttc tgcgggacga gccgggtcac
421 gcggctgacg cggagcgtcc gttgggcgac aaacaccagg acggggcaca ggtacactat
481 cttgtcaccc ggaggcgcga gggactgcag gagcttcagg gagtggcgca gctgcttcat
541 ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg gaagaaatat atttgcatgt
601 ctttagttct atgatgacac aaaccccgcc cagcgtcttg tcattggcga attcgaacac
661 gcagatgcag tcggggcggc gcggtcccag gtccacttcg catattaagg tgacgcgtgt
721 ggcctcgaac accgagcgac cctgcagcga cccgcttaaa agcttgattc ttctgacaca
781 acagtctcga acttaagctg cagaagttgg tcgtgaggca ctgggcaggt aagtatcaag
841 gttacaagac aggtttaagg agaccaatag aaactgggct tgtcgagaca gagaagactc
901 ttgcgtttct gataggcacc tattggtctt actgacatcc actttgcctt tctctccaca
961 ggtgtccact cccagttcaa ttacagctct taaggctaga gtacttaata cgactcacta
1021 taggctagcc accatggctt ccaaggtgta cgaccccgag caacgcaaac gcatgatcac
1081 tgggcctcag tggtgggctc gctgcaagca aatgaacgtg ctggactcct tcatcaacta
1141 ctatgattcc gagaagcacg ccgagaacgc cgtgattttt ctgcatggta acgctgcctc
1201 cagctacctg tggaggcacg tcgtgcctca catcgagccc gtggctagat gcatcatccc
1261 tgatctgatc ggaatgggta agtccggcaa gagcgggaat ggctcatatc gcctcctgga
1321 tcactacaag tacctcaccg cttggttcga gctgctgaac cttccaaaga aaatcatctt
1381 tgtgggccac gactgggggg cttgtctggc ctttcactac tcctacgagc accaagacaa
1441 gatcaaggcc atcgtccatg ctgagagtgt cgtggacgtg atcgagtcct gggacgagtg
1501 gcctgacatc gaggaggata tcgccctgat caagagcgaa gagggcgaga aaatggtgct
1561 tgagaataac ttcttcgtcg agaccatgct cccaagcaag atcatgcgga aactggagcc
1621 tgaggagttc gctgcctacc tggagccatt caaggagaag ggcgaggtta gacggcctac
1681 cctctcctgg cctcgcgaga tccctctcgt taagggaggc aagcccgacg tcgtccagat
1741 tgtccgcaac tacaacgcct accttcgggc cagcgacgat ctgcctaaga tgttcatcga
1801 gtccgaccct gggttctttt ccaacgctat tgtcgaggga gctaagaagt tccctaacac
1861 cgagttcgtg aaggtgaagg gcctccactt cagccaggag gacgctccag atgaaatggg
1921 taagtacatc aagagcttcg tggagcgcgt gctgaagaac gagcagtaat tctagagcgg
1981 ccgcttcgag cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
2041 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
2101 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
2161 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat
2221 aaggatccag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
2281 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
2341 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
2401 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
2461 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
2521 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
2581 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
2641 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
2701 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
2761 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
2821 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
2881 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
2941 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
3001 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
3061 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
3121 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
3181 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
3241 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
3301 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
3361 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
3421 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
3481 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
3541 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
3601 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
3661 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
3721 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
3781 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
3841 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
3901 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
3961 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
4021 tggccttttg ctcacatggc tcgac
//
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