| 质粒名称: | pBAD-HisB-LSSmScarlet3 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000175 |
| 产品用途: | Expresses the LSSmScarlet3 chemically stable red fluorescent protein with a large Stock's shift in bacterial cells |
| 存储实验室: | Laboratory of Molecular Design |
|
载体骨架基本信息 |
|
| 载体名称: | pBAD |
| 表达系统: | 细菌表达 |
| 启动子: |
arabinose
|
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Mach1, BW25113 |
|
插入基因信息 |
|
| 插入基因名称: | LSSmScarlet3 |
| 物种来源: | Synthetic |
| 密码子优化: | 细菌表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
LSSmScarlet2 and LSSmScarlet3, Chemically Stable Genetically Encoded Red Fluorescent Proteins with a Large Stokes' Shift
Subach O.M., Vlaskina A.V., Agapova Y.K., Piatkevich K.D., Patrushev M.V., Samygina V.R., Subach F.V.
Int. J. Mol. Sci. 23, 11051 (2022)
|
买家导航
pBAD-HisB-LSSmScarlet3载体质粒基本信息
pBAD-HisB-LSSmScarlet3质粒图谱载体图谱和pBAD-HisB-LSSmScarlet3载体序列质粒序列多克隆位点信息
pBAD-HisB-LSSmScarlet3质粒载体简介
pBAD-HisB-LSSmScarlet3质粒序列载体序列
LOCUS pBAD-HisB-LSSmScarlet3 4773 bp DNA circular SYN 22-APR-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4773)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4773
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 120..285
/gene="araBAD"
/label=araBAD promoter
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction"
CDS 331..348
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
CDS 352..384
/codon_start=1
/product="leader peptide from bacteriophage T7 gene 10"
/label=T7 tag (gene 10 leader)
/note="promotes efficient translation in E. coli"
/translation="MASMTGGQQMG"
CDS 388..411
/codon_start=1
/product="Xpress(TM) epitope tag, including an enterokinase
recognition and cleavage site"
/label=Xpress(TM) tag
/translation="DLYDDDDK"
CDS 430..1128
/codon_start=1
/label=LSSmScarlet3
/translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYHKQSFPEGFKWERVMNFEDGG
AVTVTQDTSLEDGTLIYEVKLRGTNFPPDGPVMQKKTMGLEADTERLYPEDGVLKGDIK
MALRLKDGGRYLAHVRTTYKAKKPVLMPGAYNVDRKLDITSHNEDYTVVEQFERSEGRH
STGDMDELYK"
terminator 1344..1430
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 1522..1549
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
promoter 1568..1659
/gene="bla"
/label=AmpR promoter
CDS 1660..2520
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 2691..3279
/direction=RIGHT
/label=ori
/note="high-copy-number colE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 3465..3605
/label=bom
/note="basis of mobility region from pBR322"
CDS complement(3869..4747)
/codon_start=1
/gene="araC"
/product="L-arabinose regulatory protein"
/label=araC
/translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
EKVNDVAVKLS"
ORIGIN
1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc
301 taacaggagg aattaaccat ggggggttct catcatcatc atcatcatgg tatggctagc
361 atgactggtg gacagcaaat gggtcgggat ctgtacgacg atgacgataa ggatccgagc
421 tcgagatcta tggtgagcaa gggcgaggca gtgatcaagg agttcatgcg gttcaaggtg
481 cacatggagg gctccatgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
541 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggtcc actgcccttc
601 tcctgggaca tcctgtcccc tcagttcatg tacggctcca gggccttcac caagcacccc
661 gccgatatcc ccgactacca taagcagtcc ttccccgagg gcttcaagtg ggagcgcgtg
721 atgaacttcg aggacggcgg cgccgtgacc gtgacccagg acacctccct ggaggacggc
781 accctgatct acgaggtgaa gctccgcggc accaacttcc ctcctgacgg ccccgtaatg
841 cagaagaaga ctatgggctt ggaagcggac accgagcggt tgtaccccga ggacggcgtg
901 ctgaagggcg acattaagat ggccctgcgc ctgaaggacg gcggccgcta cctggcacac
961 gtcaggacca cctacaaggc caagaagccc gtgttgatgc ccggcgccta caacgtcgac
1021 cgcaagttgg acatcacctc ccacaacgag gactacaccg tggtggaaca gttcgaacgc
1081 tccgagggcc gccactccac cggcgacatg gacgagctgt acaagtaaga attcgaagct
1141 tggctgtttt ggcggatgag agaagatttt cagcctgata cagattaaat cagaacgcag
1201 aagcggtctg ataaaacaga atttgcctgg cggcagtagc gcggtggtcc cacctgaccc
1261 catgccgaac tcagaagtga aacgccgtag cgccgatggt agtgtggggt ctccccatgc
1321 gagagtaggg aactgccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
1381 ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgggag
1441 cggatttgaa cgttgcgaag caacggcccg gagggtggcg ggcaggacgc ccgccataaa
1501 ctgccaggca tcaaattaag cagaaggcca tcctgacgga tggccttttt gcgtttctac
1561 aaactctttt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
1621 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
1681 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
1741 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
1801 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
1861 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
1921 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
1981 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
2041 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
2101 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
2161 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
2221 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
2281 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
2341 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
2401 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
2461 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
2521 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
2581 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
2641 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
2701 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
2761 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
2821 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
2881 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
2941 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
3001 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
3061 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
3121 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
3181 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
3241 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
3301 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
3361 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
3421 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt
3481 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc
3541 tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg
3601 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
3661 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
3721 cgtcatcacc gaaacgcgcg aggcagcaga tcaattcgcg cgcgaaggcg aagcggcatg
3781 cataatgtgc ctgtcaaatg gacgaagcag ggattctgca aaccctatgc tactccgtca
3841 agccgtcaat tgtctgattc gttaccaatt atgacaactt gacggctaca tcattcactt
3901 tttcttcaca accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaataccc
3961 gcgagaaata gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc
4021 gggtggtgct caaaagcagc ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga
4081 cgctaatccc taactgctgg cggaaaagat gtgacagacg cgacggcgac aagcaaacat
4141 gctgtgcgac gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac
4201 aagcctcgcg tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc
4261 gccgcagtaa caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc
4321 cttgcccggc gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat
4381 ccgggcgaaa gaaccccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt
4441 aggcgcgcgg acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac
4501 cgtagtgatg aatctctcct ggcgggaaca gcaaaatatc acccggtcgg caaacaaatt
4561 ctcgtccctg atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt
4621 tcattcccag cggtcggtcg ataaaaaaat cgagataacc gttggcctca atcggcgtta
4681 aacccgccac cagatgggca ttaaacgagt atcccggcag caggggatca ttttgcgctt
4741 cagccatact tttcatactc ccgccattca gag
//
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