PiggyBac-CMV-P2RX7(human)-Puro

LOCUS       PiggyBac-CMV-P2RX7(human)-Puro             8088 bp DNA     circular SYN 23-DEC-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8088)
  AUTHORS   Triple Threat
  TITLE     Direct Submission
  JOURNAL   Exported Jul 29, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8088
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    874..936
                     /label=piggyBac right (3') inverted repeat
     promoter        1292..1495
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    1578..1586
                     /label=Feature 17
     promoter        3416..3627
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             3640..3908
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             3943..4542
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     polyA_signal    4653..4779
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    5542..5576
                     /label=piggyBac left (5') inverted repeat
     primer_bind     complement(6067..6083)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6091..6107
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6115..6145)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6160..6181
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(6469..7057)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7228..8088)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctcggcct
     1201 ccacggccac tagtattatg cccagtacat gaccttatgg gactttccta cttggcagta
     1261 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
     1321 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
     1381 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
     1441 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtt tatataagca gagctcgttt
     1501 agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagatt
     1561 ctagagctag cgaattcgcc accatgccgg cctgctgcag ctgcagtgat gttttccagt
     1621 atgagacgaa caaagtcact cggatccaga gcatgaatta tggcaccatt aagtggttct
     1681 tccacgtgat catcttttcc tacgtttgct ttgctctggt gagtgacaag ctgtaccagc
     1741 ggaaagagcc tgtcatcagt tctgtgcaca ccaaggtgaa ggggatagca gaggtgaaag
     1801 aggagatcgt ggagaatgga gtgaagaagt tggtgcacag tgtctttgac accgcagact
     1861 acaccttccc tttgcagggg aactctttct tcgtgatgac aaactttctc aaaacagaag
     1921 gccaagagca gcggttgtgt cccgagtatc ccacccgcag gacgctctgt tcctctgacc
     1981 gaggttgtaa aaagggatgg atggacccgc agagcaaagg aattcagacc ggaaggtgtg
     2041 tagtgtatga agggaaccag aagacctgtg aagtctctgc ctggtgcccc atcgaggcag
     2101 tggaagaggc cccccggcct gctctcttga acagtgccga aaacttcact gtgctcatca
     2161 agaacaatat cgacttcccc ggccacaact acaccacgag aaacatcctg ccaggtttaa
     2221 acatcacttg taccttccac aagactcaga atccacagtg tcccattttc cgactaggag
     2281 acatcttccg agaaacaggc gataattttt cagatgtggc aattcagggc ggaataatgg
     2341 gcattgagat ctactgggac tgcaacctag accgttggtt ccatcactgc cgtcccaaat
     2401 acagtttccg tcgccttgac gacaagacca ccaacgtgtc cttgtaccct ggctacaact
     2461 tcagatacgc caagtactac aaggaaaaca atgttgagaa acggactctg ataaaagtct
     2521 tcgggatccg ttttgacatc ctggtttttg gcaccggagg aaaatttgac attatccagc
     2581 tggttgtgta catcggctca accctctcct acttcggtct ggccgctgtg ttcatcgact
     2641 tcctcatcga cacttactcc agtaactgct gtcgctccca tatttatccc tggtgcaagt
     2701 gctgtcagcc ctgtgtggtc aacgaatact actacaggaa gaagtgcgag tccattgtgg
     2761 agccaaagcc gacattaaag tatgtgtcct ttgtggatga atcccacatt aggatggtga
     2821 accagcagct actagggaga agtctgcaag atgtcaaggg ccaagaagtc ccaagacctg
     2881 cgatggactt cacagatttg tccaggctgc ccctggccct ccatgacaca cccccgattc
     2941 ctggacaacc agaggagata cagctgctta gaaaggaggc gactcctaga tccagggata
     3001 gccccgtctg gtgccagtgt ggaagctgcc tcccatctca actccctgag agccacaggt
     3061 gcctggagga gctgtgctgc cggaaaaagc cgggggcctg catcaccacc tcagagctgt
     3121 tcaggaagct ggtcctgtcc agacacgtcc tgcagttcct cctgctctac caggagccct
     3181 tgctggcgct ggatgtggat tccaccaaca gccggctgcg gcactgtgcc tacaggtgct
     3241 acgccacctg gcgcttcggc tcccaggaca tggctgactt tgccatcctg cccagctgct
     3301 gccgctggag gatccggaaa gagtttccga agagtgaagg gcagtacagt ggcttcaaga
     3361 gtccttactg ataggcggcc gcgaaggatc tgcgatcgct ccggtgcccg tcagtgggca
     3421 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt
     3481 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
     3541 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
     3601 cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca
     3661 cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct
     3721 cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg
     3781 agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg
     3841 ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt
     3901 tacagatcca agctgtgacc ggcgcctacg ctagacgcca ccatgaccga gtacaagccc
     3961 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     4021 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     4081 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     4141 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     4201 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     4261 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     4321 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     4381 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     4441 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     4501 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaaatcaacc tctggattac
     4561 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     4621 tacgctgctt taatgccttt gtatcatgcg ttaactaaac ttgtttattg cagcttataa
     4681 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     4741 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga attgactcaa
     4801 atgatgtcaa ttagtctatc agaagctcat ctggtctccc ttccggggga caagacatcc
     4861 ctgtttaata tttaaacagc agtgttccca aactgggttc ttatatccct tgctctggtc
     4921 aaccaggttg cagggtttcc tgtcctcaca ggaacgaagt ccctaaagaa acagtggcag
     4981 ccaggtttag ccccggaatt gactggattc cttttttagg gcccattggt atggcttttt
     5041 ccccgtatcc ccccaggtgt ctgcaggctc aaagagcagc gagaagcgtt cagaggaaag
     5101 cgatcccgtg ccaccttccc cgtgcccggg ctgtccccgc acgctgccgg ctcggggatg
     5161 cggggggagc gccggaccgg agcggagccc cgggcggctc gctgctgccc cctagcgggg
     5221 gagggacgta attacatccc tgggggcttt gggggggggc tgtccctgat atctataaca
     5281 agaaaatata tatataataa gttatcacgt aagtagaaca tgaaataaca atataattat
     5341 cgtatgagtt aaatcttaaa agtcacgtaa aagataatca tgcgtcattt tgactcacgc
     5401 ggtcgttata gttcaaaatc agtgacactt accgcattga caagcacgcc tcacgggagc
     5461 tccaagcggc gactgagatg tcctaaatgc acagcgacgg attcgcgcta tttagaaaga
     5521 gagagcaata tttcaagaat gcatgcgtca attttacgca gactatcttt ctagggttaa
     5581 tctagctgca tcaggatcat atcgtcgggt cttttttccg gctcagtcat cgcccaagct
     5641 ggcgctatct gggcatcggg gaggaagaag cccgtgcctt ttcccgcgag gttgaagcgg
     5701 catggaaaga gtttgccgag gatgactgct gctgcattga cgttgagcga aaacgcacgt
     5761 ttaccatgat gattcgggaa ggtgtggcca tgcacgcctt taacggtgaa ctgttcgttc
     5821 aggccacctg ggataccagt tcgtcgcggc ttttccggac acagttccgg atggtcagcc
     5881 cgaagcgcat cagcaacccg aacaataccg gcgacagccg gaactgccgt gccggtgtgc
     5941 agattaatga cagcggtgcg gcgctgggat attacgtcag cgaggacggg tatcctggct
     6001 ggatgccgca gaaatggaca tggatacccc gtgagttacc cggcgggcgc gcttggcgta
     6061 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     6121 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     6181 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     6241 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     6301 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     6361 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     6421 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     6481 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     6541 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     6601 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     6661 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     6721 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     6781 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     6841 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     6901 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     6961 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     7021 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     7081 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     7141 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     7201 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     7261 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     7321 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     7381 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     7441 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     7501 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     7561 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     7621 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     7681 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     7741 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     7801 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     7861 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     7921 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     7981 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     8041 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcat
//