| 质粒名称: | pDG205 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002904 |
| 产品用途: | Mammalian PhCMV-driven constitutive expression vector for UbH-tagged NanoLuc (PhCMV-UbH-NanoLuc-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | UbH, NanoLuc |
| 物种来源: | synthetic |
| 密码子优化: | 未优化 |
pDG205
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pDG205载体质粒基本信息
pDG205质粒图谱载体图谱和pDG205载体序列质粒序列多克隆位点信息
pDG205质粒载体简介
pDG205质粒序列载体序列
LOCUS pDG205 6257 bp DNA circular SYN 21-JUL-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6257)
AUTHORS Westlake University
TITLE Direct Submission
JOURNAL Exported Sep 12, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6257
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 826..845
/label=Primer 1
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 887..944
/label=oSCL1108
misc_feature 895..911
/label=MCS
/note="multiple cloning site"
primer_bind 897..944
/label=oSCL1314
primer_bind 898..936
/label=Primer 14
primer_bind 898..931
/label=Primer 7a
primer_bind 899..936
/label=Primer 7
primer_bind 899..936
/label=Primer 16
primer_bind 899..936
/label=Primer 26
primer_bind complement(899..919)
/label=Primer 25
primer_bind 911..936
/label=Primer 1a
primer_bind 911..936
/label=Primer 9
promoter 911
/label=P_SV40
primer_bind 917..944
/label=oSCL1846
primer_bind 917..940
/label=oSCL1848
primer_bind 917..936
/label=Primer 3
primer_bind 921..944
/label=oSCL084
misc_feature 923..991
/label=Secretory (IgK)
/label=nonstandard type: signal
primer_bind 990..1030
/label=Primer 9a
primer_bind complement(990..1009)
/label=Primer 8
CDS 1010..1243
/codon_start=1
/transl_table=11
/product="UbR-tTA-Dendra2"
/label=UbR-tTA-Dendra2
/protein_id="QCO92762.1"
/translation="MTSQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRL
IFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
primer_bind 1010..1046
/label=Primer 5
primer_bind 1010..1025
/label=Primer 7a
misc_feature 1019..1243
/label=UbR
/note="UbR"
primer_bind 1027..1085
/label=Primer 3a
primer_bind 1066..1124
/label=Primer 1`b
primer_bind complement(1105..1163)
/label=Primer 2a
primer_bind 1126..1137
/label=Primer 5
primer_bind complement(1144..1202)
/label=Primer 4
primer_bind complement(1183..1237)
/label=Primer 6
primer_bind complement(1218..1264)
/label=Primer 8a
primer_bind complement(1218..1264)
/label=Primer 10
primer_bind complement(1224..1266)
/label=Primer 11
primer_bind complement(1224..1266)
/label=Primer 12
primer_bind complement(1224..1266)
/label=Primer 19
primer_bind complement(1224..1266)
/label=Primer 13
primer_bind complement(1224..1266)
/label=Primer 21
primer_bind complement(1224..1266)
/label=Primer 22
primer_bind complement(1224..1266)
/label=Fragment.REV
primer_bind complement(1224..1264)
/label=Primer 20
primer_bind complement(1224..1264)
/label=Primer 23
primer_bind complement(1224..1264)
/label=Primer 24
primer_bind 1247..1287
/label=Primer 5a
primer_bind 1247..1284
/label=Primer 17
primer_bind complement(1247..1264)
/label=Primer 15
misc_feature 1268..1777
/label=NLuc (nano Luciferase)
/label=nonstandard type: reporter
misc_feature 1298..1300
/label=cyt-STOP (TGG->TGA)
primer_bind complement(1757..1780)
/label=oSCL1849
primer_bind complement(1758..1825)
/label=Primer 10a
primer_bind complement(1758..1798)
/label=Primer 18
primer_bind complement(1758..1797)
/label=Primer 6a
primer_bind complement(1758..1780)
/label=Primer 4a
primer_bind complement(1758..1777)
/label=Primer 15
primer_bind complement(1758..1777)
/label=Primer 27
primer_bind complement(1767..1777)
/label=oSCL085
primer_bind complement(1767..1777)
/label=oSCL1109
misc_feature 1786..1831
/label=MCS
/note="multiple cloning site"
primer_bind complement(1831..1850)
/label=Primer 2
polyA_signal 1857..2081
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 2127..2555
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2569..2898
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2749..2884
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2884..2903
/label=Primer 1a
CDS 2965..3759
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin)"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3933..4054
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(4505..5090)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5261..6121)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(6122..6226)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttgcca ccatgactag tgagacagac acactcctgc tatgggtact
961 gctgctctgg gttccaggtt ccactggtga cgctagtggt ggttctggta tgactagtca
1021 gattttcgtg aagaccctga ccggcaagac catcacccta gaggtggagc ccagtgacac
1081 catcgagaac gtgaaggcca agatccagga taaagagggc atcccccctg accagcagag
1141 gctgatcttt gccggcaagc agctggaaga tggccgcacc ctctctgatt acaacatcca
1201 gaaggagtca accctgcacc tggtccttcg cctgagaggt ggccacagcg gaagtggaag
1261 cagtatggtc ttcacactcg aagatttcgt tggggactgg cgacagacag ccggctacaa
1321 cctggaccaa gtccttgaac agggaggtgt gtccagtttg tttcagaatc tcggggtgtc
1381 cgtaactccg atccaaagga ttgtcctgag cggtgaaaat gggctgaaga tcgacatcca
1441 tgtcatcatc ccgtatgaag gtctgagcgg cgaccaaatg ggccagatcg aaaaaatttt
1501 taaggtggtg taccctgtgg atgatcatca ctttaaggtg atcctgcact atggcacact
1561 ggtaatcgac ggggttacgc cgaacatgat cgactatttc ggacggccgt atgaaggcat
1621 cgccgtgttc gacggcaaaa agatcactgt aacagggacc ctgtggaacg gcaacaaaat
1681 tatcgacgag cgcctgatca accccgacgg ctccctgctg ttccgagtaa ccatcaacgg
1741 agtgaccggc tggcggctgt gcgaacgcat tctggcgtga gaattctgca gatatccagc
1801 acagtggcgg ccgctcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt
1861 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
1921 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
1981 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
2041 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
2101 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
2161 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
2221 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
2281 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
2341 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
2401 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
2461 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
2521 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
2581 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
2641 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
2701 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
2761 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
2821 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
2881 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa
2941 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
3001 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
3061 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
3121 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
3181 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
3241 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
3301 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
3361 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
3421 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
3481 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
3541 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
3601 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
3661 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
3721 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
3781 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
3841 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
3901 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
3961 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
4021 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
4081 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
4141 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
4201 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
4261 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
4321 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
4381 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
4441 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
4501 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
4561 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
4621 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
4681 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
4741 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
4801 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
4861 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
4921 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
4981 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
5041 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
5101 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
5161 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
5221 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
5281 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
5341 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
5401 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
5461 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
5521 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
5581 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
5641 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
5701 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
5761 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
5821 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
5881 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
5941 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
6001 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
6061 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
6121 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
6181 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
6241 aagtgccacc tgacgtc
//
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