| 质粒名称: | mPiezo1-IRES-eGFP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 80925 |
| 存储实验室: | Ardem Patapoutian |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 载体抗性: | 氨苄青霉素 |
| 完整质粒大小: | 14386 bp |
| 载体修饰: | Ires-eGFP inserted |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | mouse Piezo1 CDS |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 7644 bp |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | NotI (未破坏) |
| 3' 克隆位点: | HindIII（在克隆过程中被销毁） |
| 5' 测序引物 | cgcaaatgggcggtaggcgtg |
|
文献引用方法 |
|
| 材料和方法部分: | mPiezo1-IRES-eGFP was a gift from Ardem Patapoutian (Addgene plasmid # 80925 ; http://n2t.net/addgene:80925 ; RRID:Addgene_80925) |
| 参考文献部分: | Piezo1 and Piezo2 are essential components of distinct mechanically activated cation channels. Coste B, Mathur J, Schmidt M, Earley TJ, Ranade S, Petrus MJ, Dubin AE, Patapoutian A. Science. 2010 Oct 1;330(6000):55-60. doi: 10.1126/science.1193270. Epub 2010 Sep 2. 10.1126/science.1193270 PubMed 20813920 |
买家导航
mPiezo1-IRES-eGFP载体质粒基本信息
mPiezo1-IRES-eGFP质粒图谱载体图谱和mPiezo1-IRES-eGFP载体序列质粒序列多克隆位点信息
mPiezo1-IRES-eGFP质粒载体简介
mPiezo1-IRES-eGFP质粒序列载体序列
LOCUS mPiezo1-IRES-eGFP 14389 bp ds-DNA circular SYN 07-MAY-2019
DEFINITION expresses mouse Piezo1 with GFP.
ACCESSION .
VERSION .
KEYWORDS mPiezo1-IRES-eGFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 14389)
AUTHORS Coste B, Mathur J, Schmidt M, Earley TJ, Ranade S, Petrus MJ, Dubin
AE, Patapoutian A
TITLE Piezo1 and Piezo2 are essential components of distinct mechanically
activated cation channels.
JOURNAL Science. 2010 Oct 1;330(6000):55-60. doi: 10.1126/science.1193270.
Epub 2010 Sep 2.
PUBMED 20813920
REFERENCE 2 (bases 1 to 14389)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 7, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..14389
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 124..503
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 504..707
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 658..678
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 752..771
/label=T7
/note="T7 promoter, forward primer"
promoter 752..770
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
regulatory 823..832
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
misc_feature 8528..9114
/label=IRES2
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(8695..8712)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 8922..8941
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 9115..9834
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(9160..9181)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(9421..9440)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 9768..9789
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind complement(9869..9886)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 9875..10099
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 10145..10573
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(10232..10251)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 10442..10463
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(10582..10602)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 10587..10916
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 10767..10902
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 10829..10848
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 10983..11777
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(11037..11056)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 11647..11666
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 11951..12072
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(11988..12007)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 12042..12061
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(12121..12137)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(12121..12137)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(12134..12156)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 12145..12161
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(12169..12199)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 12214..12235
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(12352..12369)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(12523..13111)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(12603..12622)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(13282..14142)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 13905..13924
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(14143..14247)
/gene="bla"
/label=AmpR promoter
primer_bind complement(14322..14341)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
61 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc
121 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
181 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
241 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
301 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
361 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
421 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
481 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
541 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
601 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
661 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta
721 gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag
781 ctggctagcg tttaaacggg ccctctagac tcgagcggcc gcgccaccat ggagccgcac
841 gtgctgggcg ccgggctcta ctggctgttg ctgccctgca cgctcctggc ggcctccctg
901 ttacgcttca atgctctctc gctggtctat ttgctgtttc tactgctgct gccctggctt
961 ccaggcccct caagacacag cataccaggt cacacaggtc gcctgctccg tgcactactc
1021 tgcctcagcc tcctcttcct ggtggcccac cttgcctttc agatatgcct acacaccgtg
1081 cctcacctgg accagtttct gggacaaaac ggtagccttt gggtgaaggt gtctcaacac
1141 ataggggtta cgaggctgga cctgaaggac atctttaaca ccaccaggct ggtagcacct
1201 gacctgggag tgctgctggc gtcctccctt tgccttggcc tctgtggacg cctcacgagg
1261 aaagccgggc agagtcggcg cacccaggag ctgcaggatg atgacgatga cgatgatgac
1321 gacgatgaag acatagatgc tgccccagcc gtggggctga agggagcccc tgccctggca
1381 accaaacgca ggctgtggct ggcctcccgc ttccgggtca cggcccactg gctgctgatg
1441 acctctggac ggacgctggt cattgtgttg ctggccctgg caggcatagc ccacccttcg
1501 gccttctcca gcatctacct ggtggtgttc ctggccatct gcacctggtg gtcctgtcac
1561 tttcctctca gccccctggg ctttaacacc ctctgtgtca tggtgagctg ttttggtgcc
1621 ggccatctca tttgcctata ctgctatcag acaccattta tccaggacat gttaccccct
1681 gggaacatct gggccaggct atttggtctc aagaacttcg tagacctccc taactactcc
1741 agccccaacg ccctggtact caacactaag cacgcctggc ccatctatgt gagtcccgga
1801 atcctgctgc tgctatatta cacagccacc tctctcctga agctccacaa gagctgtccc
1861 tcagagctga ggaaggagac acccagggag gatgaggagc atgagctgga actggaccac
1921 ctggagccag agccccaggc tagggacgcc acccagggtg agatgcccat gaccacggaa
1981 cctgaccttg acaactgcac cgtgcatgta ctaaccagcc agagccctgt ccgccagcgt
2041 ccagttcgcc ccaggctggc tgagctgaaa gagatgtcac cgctacatgg cctgggccac
2101 ctcatcatgg accagagcta tgtgtgtgcc ctgattgcca tgatggtgtg gagcatcatg
2161 taccacagtt ggctgacctt cgtcctgctg ctctgggcct gcctcatctg gacggtgcgt
2221 agccgtcacc agctggctat gctctgctcg ccctgcatcc tgctgtatgg gctgacgctc
2281 tgctgcctga ggtatgtgtg ggccatggaa cttcctgagc tgcccaccac cctgggccct
2341 gtcagcctgc accagttggg actggaacac acacgctacc cttgcctgga cctcggtgcc
2401 atgctcctct atctgctcac attctggctc cttctgcgtc agttcgtgaa ggagaagctg
2461 ctgaagaagc aaaaggtgcc tgcggcgctg ctggaggtca cggtggccga cactgagccc
2521 acacagaccc agacgctgct gcggagcctg ggggagctgg tcaccggcat ctacgtcaaa
2581 tactggatct atgtgtgcgc cggcatgttc attgtggtca gcttcgccgg ccgcctggtg
2641 gtctacaaaa tcgtctacat gttcctcttc ctgctgtgcc tcaccttgtt ccaggtctac
2701 tacaccctgt ggaggaagct gctacgtgtc ttctggtggc tcgtggtggc ctatacaatg
2761 ctcgtgctca tcgctgtgta caccttccag ttccaggact tccccaccta ttggcgcaac
2821 ctcacgggct tcacagacga gcagttgggc gacctgggcc tggagcagtt cagtgtgtcg
2881 gagctctttt ccagtatcct catccctggc ttcttcctgc tggcctgcat cctgcagctg
2941 cactacttcc acagaccgtt catgcagctc actgacctgg agcacgtgcc gccaccaggc
3001 acccgccacc ctcgatgggc tcacaggcag gatgcagtga gcgaggcccc tctgcttgag
3061 catcaggagg aagaggaagt cttcagggaa gatgggcaga gcatggatgg gccccaccag
3121 gccacacagg tccctgaggg tacggccagc aagtggggcc tggtggctga ccggctgctg
3181 gacctagcgg ccagcttctc ggctgtcctc acccgaatcc aggtgttcgt tcggcgcttg
3241 ctagaacttc acgtcttcaa gctggtggcc ctctacactg tctgggtggc cctgaaagaa
3301 gtgtctgtga tgaacctgct gctggtggtg ctatgggcct tcgccttgcc ctatccgcgc
3361 ttccggccca tggcttcctg cctgtccacc gtgtggacct gtatcatcat tgtatgcaag
3421 atgctctatc agctcaagat tgtcaacccg catgagtact ccagcaactg cactgagccc
3481 ttccccaaca ataccaactt gcagcctttg gagatcaacc agtctttgct gtaccgtggc
3541 cctgttgacc ctgccaactg gtttggggtg cggaagggtt accccaactt gggctatatc
3601 cagaaccacc tgcagatcct tctgttgctg gtgtttgagg ccgtggtgta ccggcgccaa
3661 gagcactacc gccggcagca ccagcaggcc cctctgcccg cccaggctgt gtgcgcagat
3721 ggcacccgcc agaggttgga ccaggaccta cttagctgcc tcaagtattt catcaacttc
3781 ttcttctaca aattcgggct ggagatctgc ttcttgatgg ccgtgaatgt gattgggcag
3841 cgtatgaact tcatggtgat cttacacggt tgctggttgg tggccatcct tacacgccgg
3901 cgccgtgagg ccatcgcccg cctctggcct aactactgtc tgttcctcac gctgttcctg
3961 ctgtaccagt acctgctgtg tttgggcatg ccccctgcac tgtgcattga ctatccatgg
4021 cgctggagca aggccatccc catgaattcc gccctcatca agtggctgta cctacctgac
4081 ttcttcagag cccccaactc caccaacctt atcagtgact tcctcctgct gctttgcgcc
4141 tcccagcagt ggcaggtctt ctcagcggag cgaacggagg agtggcaacg catggcgggc
4201 atcaacactg accacctgga gcccctgcgt ggggagccca accctatacc caacttcatc
4261 cactgcaggt cctatctgga tatgctgaag gtggccgtct tccgctacct gttctggctg
4321 gtgctcgttg tggtgtttgt tgcgggggcc acccgcataa gcatcttcgg gctggggtac
4381 ctgctagcct gcttctacct gctgctgttt ggcactaccc tgctgcagaa ggacacgcga
4441 gcccagctcg tgctgtggga ctgcctcatc ctctataatg tcactgtcat catctctaag
4501 aatatgctgt cgctcctgtc ctgtgtcttc gtggagcaaa tgcagagcaa cttctgctgg
4561 gtcatccagc tcttcagcct cgtgtgcaca gtcaaaggct actatgatcc caaagagatg
4621 atgaccaggg accgggactg cctgctgcct gtggaggagg ccgggatcat ctgggacagt
4681 atctgcttct tcttcctgct cttgcaacgg cgcatctttc tcagccacta cttcctgcat
4741 gtcagcgctg acctgaaagc cacagccctg caggcatcca ggggctttgc cctctacaat
4801 gcagccaacc tgaagagcat caacttccat cgccagattg aggagaagtc cctggcccag
4861 ctgaaaagac agatgaagcg catccgtgcc aaacaggaga agtacaggca gagccaggca
4921 agtcgtggcc aactccagtc caaagaccct caggatccca gccaggagcc agggcctgac
4981 agcccagggg gctcctcccc gccacggaga cagtggtggc gcccctggct ggaccacgcc
5041 acagtcatcc actctggcga ctacttcctg tttgagtcag atagcgagga ggaagaggag
5101 gccctacctg aggaccccag gcctgcagct cagagtgcct tccagatggc ataccaggca
5161 tgggtaacca atgcccagac agtgctgagg cagcgtcggg agcgggcacg gcaggagcgg
5221 gcagagcagc tggcttctgg aggtgacttg aacccagatg tggaaccagt agatgtccca
5281 gaagatgaga tggcaggccg tagccacatg atgcagcgtg tgctaagcac catgcagttc
5341 ctgtgggtgc tgggccaggc cacggtagac gggctgacgc gctggctgcg tgcattcacg
5401 aagcaccacc gcaccatgag cgatgtgctg tgcgcagagc gctacctgct cacccaggag
5461 cttcttcggg ttggagaggt acgccgaggt gtgctggacc agctttatgt gggtgaagat
5521 gaggccacat tgtcaggtcc cgtggagacc cgggatggac ccagcacagc ctcaagtggg
5581 ctgggagccg aagagccttt gagtagcatg acagacgaca ccagcagccc cctgagcaca
5641 ggctataaca cccgcagtgg cagtgaggag attgtcaccg acgctgggga cctccaggct
5701 gggacctccc tgcacggctc ccaagagctt ttagccaatg ctcgtacccg gatgcgcacg
5761 gccagcgagc tgctactgga taggcgcctg catatccctg agctggagga ggccgagcgg
5821 tttgaggcac agcagggccg gactctgcgg ctgctcaggg ctgggtacca gtgcgtggcg
5881 gcacactcgg agctgctctg ttacttcatc atcatcctta accacatggt gacagcctcg
5941 gctgcctccc tggtgctgcc cgtgcttgtg ttcctgtggg ccatgctgac catcccgagg
6001 cctagcaagc gcttttggat gacagctatc gtcttcactg aggtcatggt ggtcaccaaa
6061 tacctgttcc agttcggctt cttcccctgg aacagctacg ttgtgctgcg gcgctatgag
6121 aacaagccct acttccctcc gcgaatcctg ggccttgaga aaacggacag ctacatcaag
6181 tatgacctgg tgcagctcat ggccctcttc ttccaccgct cgcagctact gtgttatggc
6241 ctctgggacc atgaggagga tcgctatccc aaggaccatt gcaggagtag tgtgaaggac
6301 cgggaggcca aggaagagcc agaagctaag ctggaatcgc agtctgagac aggcactggg
6361 catcccaagg agccagtgtt ggccggtact cccagggacc acatccaagg gaaaggaagt
6421 attagatcca aggatgttat ccaagatccc ccagaggacc ttaagccccg gcacacgagg
6481 cacatcagca tacgcttcag gaggcgcaag gagactccag gacccaaagg aacagcagtc
6541 atggagactg agcacgagga gggagaagga aaagaaacta cagagagaaa gaggccgcgt
6601 cacactcaag aaaaatcgaa gtttcgggag agaatgaagg cagctgggcg ccggctgcag
6661 agcttctgtg tgtcactggc ccagagcttc taccaaccct tgcagcgttt cttccatgac
6721 attctgcaca caaagtaccg ggcggccacc gacgtctacg ccctcatgtt cctggccgat
6781 attgtcgaca tcatcatcat catctttggt ttttgggctt ttgggaagca ctctgcagcc
6841 acagacattg catcctcgct gtcagatgac caggtgccac aggccttcct gttcatgctg
6901 ctggtccagt ttggcaccat ggtcatcgac cgtgccctct acctgcgcaa gactgtcctg
6961 ggaaagctgg cctttcaggt ggtcctggtg gtggcgattc acatctggat gttctttatc
7021 ttaccggctg tcactgagag gatgttcagc cagaatgcgg tggcacagct gtggtacttc
7081 gtcaagtgca tttactttgc cctgtccgcc taccagatcc gctgtggcta ccccacccgt
7141 atcttgggca acttcctcac caagaaatac aaccatctaa acctcttcct tttccagggg
7201 ttccgtctag tgccgttcct ggtggagctg cgggccgtca tggactgggt gtggaccgac
7261 accacgctgt ccctgtccaa ctggatgtgt gtggaagaca tctatgccaa catctttatc
7321 atcaagtgca gccgagagac agagaagaaa tacccccagc ccaaggggca gaagaagaag
7381 aaaattgtca agtatggtat gggaggcctc attatcctct tcctcatcgc catcatctgg
7441 ttccctctgc tcttcatgtc actgatccgc tctgtggtcg gggtcgtcaa ccagcccatt
7501 gatgtcaccg tcaccctcaa gctaggcggc tatgagccac tgttcaccat gagcgcccag
7561 cagccatcca ttgtgccatt cacaccccag gcctacgagg agctgtccca gcagtttgac
7621 ccctatccac tagccatgca gttcattagc cagtacagtc ctgaggacat cgtcactgca
7681 cagatcgagg gcagctcggg ggcgctgtgg cgcatcagcc cacccagccg agcccagatg
7741 aagcaggagc tgtacaacgg cacagccgac attacactgc gctttacctg gaatttccaa
7801 agggacctgg ccaagggtgg cactgtggag tatactaatg agaagcacac cttggagctg
7861 gcccccaaca gtacggcacg aaggcagctg gcccaactgc tcgagggcag acctgaccag
7921 tcagtggtca ttccccatct cttccccaag tacattcgtg ctcccaatgg gcctgaagcc
7981 aaccctgtga agcagctgca gccagatgag gaagaggact accttggtgt gcgcatccag
8041 ctgcggaggg agcaagtggg cacaggggcc tctggggagc aagcgggcac caaggcctcc
8101 gacttcctcg agtggtgggt catcgagctg caggactgca aggctgactg caacctgctg
8161 cccatggtca tcttcagtga caaggtcagc ccacctagcc tgggcttcct ggccggctac
8221 gggattgtgg ggctgtacgt ctccatcgtg ctggtggttg gcaagtttgt gcggggcttc
8281 ttcagcgaga tctctcactc catcatgttc gaggaactgc cgtgtgtgga ccgcatcctc
8341 aagctgtgcc aggacatctt cttggtgcgc gagacccggg agctggagct ggaggaggag
8401 ctatacgcca agctcatctt cctgtaccga tctccagaga ccatgattaa gtggacacgt
8461 gagagggagt agaagcttgg cgcgccttaa ttaactgcag tcgacggtac cgcgggcccg
8521 ggatccgccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag
8581 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
8641 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
8701 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
8761 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
8821 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
8881 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
8941 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
9001 ctcggtacac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga
9061 accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggccac aaccatggtg
9121 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
9181 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
9241 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
9301 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
9361 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
9421 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
9481 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
9541 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
9601 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
9661 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
9721 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
9781 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaggccgg
9841 ccaagcttaa gtttaaaccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
9901 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
9961 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
10021 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
10081 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
10141 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
10201 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
10261 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
10321 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
10381 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
10441 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
10501 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
10561 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
10621 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
10681 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
10741 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
10801 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
10861 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
10921 tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc
10981 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
11041 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
11101 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
11161 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
11221 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
11281 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
11341 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
11401 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
11461 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
11521 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
11581 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
11641 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
11701 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
11761 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
11821 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
11881 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
11941 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
12001 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
12061 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
12121 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
12181 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
12241 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
12301 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
12361 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
12421 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
12481 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
12541 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
12601 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
12661 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
12721 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
12781 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
12841 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
12901 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
12961 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
13021 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
13081 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
13141 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
13201 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
13261 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
13321 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
13381 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
13441 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
13501 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
13561 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
13621 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
13681 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
13741 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
13801 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
13861 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
13921 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
13981 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
14041 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
14101 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
14161 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
14221 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
14281 cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc
14341 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctg
//
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