pCMV-T7-xCas9(3.7)-P2A-EGFP

LOCUS       pCMV-T7-xCas9(3.7)-P2A-EGFP (RTW3576)                8451 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  CMV and T7 promoter expression plasmid for human codon optimized 
            xCas9(3.7)(A262T/R324L/S409I/E480K/E543D/M694I/E1219V) with a 
            c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-T7-xCas9(3.7)-P2A-EGFP (RTW3576)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8451)
  AUTHORS   Walton RT, Christie KA, Whittaker MN, Kleinstiver BP
  TITLE     Unconstrained genome targeting with near-PAMless engineered 
            CRISPR-Cas9 variants.
  JOURNAL   Science. 2020 Apr 17;368(6488):290-296. doi: 
            10.1126/science.aba8853. Epub 2020 Mar 26.
  PUBMED    32217751
REFERENCE   2  (bases 1 to 8451)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8451
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(79..667)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(159..178)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(838..1698)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1461..1480
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1699..1803)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1882..1901)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2073..2452
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2453..2656
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2607..2627
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2698..2717
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2698..2716
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2724..2733
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             2730..6833
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDTKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKLYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGIIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEKVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGDQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFI
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGVLQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6888..6908
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6921..6986
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             7005..7061
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7062..7778
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7107..7128)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7368..7387)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7715..7736
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7787..7804
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7827..7844)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7833..8057
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8128..8144)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8128..8144)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8141..8163)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8152..8168
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8176..8206)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8221..8242
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8359..8376)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
       61 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
      121 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
      181 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
      241 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
      301 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
      361 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
      421 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
      481 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
      541 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
      601 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
      661 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
      721 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
      781 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
      841 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
      901 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
      961 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     1021 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     1081 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     1141 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     1201 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     1261 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     1321 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     1381 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     1441 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     1501 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     1561 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     1621 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     1681 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     1741 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     1801 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc gatctcccga
     1861 tcccctaggg tcgactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct
     1921 gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac
     1981 aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct
     2041 gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata
     2101 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
     2161 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     2221 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     2281 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     2341 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     2401 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
     2461 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     2521 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     2581 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
     2641 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagagatccg cggccgctaa
     2701 tacgactcac tatagggaga gccgccacca tggacaagaa gtacagcatc ggcctggaca
     2761 tcggcaccaa ctctgtgggc tgggccgtga tcaccgacga gtacaaggtg cccagcaaga
     2821 aattcaaggt gctgggcaac accgaccggc acagcatcaa gaagaacctg atcggagccc
     2881 tgctgttcga cagcggcgaa acagccgagg ccacccggct gaagagaacc gccagaagaa
     2941 gatacaccag acggaagaac cggatctgct atctgcaaga gatcttcagc aacgagatgg
     3001 ccaaggtgga cgacagcttc ttccacagac tggaagagtc cttcctggtg gaagaggata
     3061 agaagcacga gcggcacccc atcttcggca acatcgtgga cgaggtggcc taccacgaga
     3121 agtaccccac catctaccac ctgagaaaga aactggtgga cagcaccgac aaggccgacc
     3181 tgcggctgat ctatctggcc ctggcccaca tgatcaagtt ccggggccac ttcctgatcg
     3241 agggcgacct gaaccccgac aacagcgacg tggacaagct gttcatccag ctggtgcaga
     3301 cctacaacca gctgttcgag gaaaacccca tcaacgccag cggcgtggac gccaaggcca
     3361 tcctgtctgc cagactgagc aagagcagac ggctggaaaa tctgatcgcc cagctgcccg
     3421 gcgagaagaa gaatggcctg ttcggaaacc tgattgccct gagcctgggc ctgaccccca
     3481 acttcaagag caacttcgac ctggccgagg ataccaaact gcagctgagc aaggacacct
     3541 acgacgacga cctggacaac ctgctggccc agatcggcga ccagtacgcc gacctgtttc
     3601 tggccgccaa gaacctgtcc gacgccatcc tgctgagcga catcctgaga gtgaacaccg
     3661 agatcaccaa ggcccccctg agcgcctcta tgatcaagct gtacgacgag caccaccagg
     3721 acctgaccct gctgaaagct ctcgtgcggc agcagctgcc tgagaagtac aaagagattt
     3781 tcttcgacca gagcaagaac ggctacgccg gctacattga cggcggagcc agccaggaag
     3841 agttctacaa gttcatcaag cccatcctgg aaaagatgga cggcaccgag gaactgctcg
     3901 tgaagctgaa cagagaggac ctgctgcgga agcagcggac cttcgacaac ggcatcatcc
     3961 cccaccagat ccacctggga gagctgcacg ccattctgcg gcggcaggaa gatttttacc
     4021 cattcctgaa ggacaaccgg gaaaagatcg agaagatcct gaccttccgc atcccctact
     4081 acgtgggccc tctggccagg ggaaacagca gattcgcctg gatgaccaga aagagcgagg
     4141 aaaccatcac cccctggaac ttcgagaagg tggtggacaa gggcgcttcc gcccagagct
     4201 tcatcgagcg gatgaccaac ttcgataaga acctgcccaa cgagaaggtg ctgcccaagc
     4261 acagcctgct gtacgagtac ttcaccgtgt ataacgagct gaccaaagtg aaatacgtga
     4321 ccgagggaat gagaaagccc gccttcctga gcggcgacca gaaaaaggcc atcgtggacc
     4381 tgctgttcaa gaccaaccgg aaagtgaccg tgaagcagct gaaagaggac tacttcaaga
     4441 aaatcgagtg cttcgactcc gtggaaatct ccggcgtgga agatcggttc aacgcctccc
     4501 tgggcacata ccacgatctg ctgaaaatta tcaaggacaa ggacttcctg gacaatgagg
     4561 aaaacgagga cattctggaa gatatcgtgc tgaccctgac actgtttgag gacagagaga
     4621 tgatcgagga acggctgaaa acctatgccc acctgttcga cgacaaagtg atgaagcagc
     4681 tgaagcggcg gagatacacc ggctggggca ggctgagccg gaagctgatc aacggcatcc
     4741 gggacaagca gtccggcaag acaatcctgg atttcctgaa gtccgacggc ttcgccaaca
     4801 gaaacttcat ccagctgatc cacgacgaca gcctgacctt taaagaggac atccagaaag
     4861 cccaggtgtc cggccagggc gatagcctgc acgagcacat tgccaatctg gccggcagcc
     4921 ccgccattaa gaagggcatc ctgcagacag tgaaggtggt ggacgagctc gtgaaagtga
     4981 tgggccggca caagcccgag aacatcgtga tcgaaatggc cagagagaac cagaccaccc
     5041 agaagggaca gaagaacagc cgcgagagaa tgaagcggat cgaagagggc atcaaagagc
     5101 tgggcagcca gatcctgaaa gaacaccccg tggaaaacac ccagctgcag aacgagaagc
     5161 tgtacctgta ctacctgcag aatgggcggg atatgtacgt ggaccaggaa ctggacatca
     5221 accggctgtc cgactacgat gtggaccata tcgtgcctca gagctttctg aaggacgact
     5281 ccatcgacaa caaggtgctg accagaagcg acaagaaccg gggcaagagc gacaacgtgc
     5341 cctccgaaga ggtcgtgaag aagatgaaga actactggcg gcagctgctg aacgccaagc
     5401 tgattaccca gagaaagttc gacaatctga ccaaggccga gagaggcggc ctgagcgaac
     5461 tggataaggc cggcttcatc aagagacagc tggtggaaac ccggcagatc acaaagcacg
     5521 tggcacagat cctggactcc cggatgaaca ctaagtacga cgagaatgac aagctgatcc
     5581 gggaagtgaa agtgatcacc ctgaagtcca agctggtgtc cgatttccgg aaggatttcc
     5641 agttttacaa agtgcgcgag atcaacaact accaccacgc ccacgacgcc tacctgaacg
     5701 ccgtcgtggg aaccgccctg atcaaaaagt accctaagct ggaaagcgag ttcgtgtacg
     5761 gcgactacaa ggtgtacgac gtgcggaaga tgatcgccaa gagcgagcag gaaatcggca
     5821 aggctaccgc caagtacttc ttctacagca acatcatgaa ctttttcaag accgagatta
     5881 ccctggccaa cggcgagatc cggaagcggc ctctgatcga gacaaacggc gaaaccgggg
     5941 agatcgtgtg ggataagggc cgggattttg ccaccgtgcg gaaagtgctg agcatgcccc
     6001 aagtgaatat cgtgaaaaag accgaggtgc agacaggcgg cttcagcaaa gagtctatcc
     6061 tgcccaagag gaacagcgat aagctgatcg ccagaaagaa ggactgggac cctaagaagt
     6121 acggcggctt cgacagcccc accgtggcct attctgtgct ggtggtggcc aaagtggaaa
     6181 agggcaagtc caagaaactg aagagtgtga aagagctgct ggggatcacc atcatggaaa
     6241 gaagcagctt cgagaagaat cccatcgact ttctggaagc caagggctac aaagaagtga
     6301 aaaaggacct gatcatcaag ctgcctaagt actccctgtt cgagctggaa aacggccgga
     6361 agagaatgct ggcctctgcc ggcgtgctgc agaagggaaa cgaactggcc ctgccctcca
     6421 aatatgtgaa cttcctgtac ctggccagcc actatgagaa gctgaagggc tcccccgagg
     6481 ataatgagca gaaacagctg tttgtggaac agcacaagca ctacctggac gagatcatcg
     6541 agcagatcag cgagttctcc aagagagtga tcctggccga cgctaatctg gacaaagtgc
     6601 tgtccgccta caacaagcac cgggataagc ccatcagaga gcaggccgag aatatcatcc
     6661 acctgtttac cctgaccaat ctgggagccc ctgccgcctt caagtacttt gacaccacca
     6721 tcgaccggaa gaggtacacc agcaccaaag aggtgctgga cgccaccctg atccaccaga
     6781 gcatcaccgg cctgtacgag acacggatcg acctgtctca gctgggaggc gacggatccg
     6841 gcggaggcgg aagcgggaaa agaaccgccg acggcagcga attcgagccc aagaagaaga
     6901 ggaaagtctc gagcggaggc gactacaaag accatgacgg tgattataaa gatcatgaca
     6961 tcgattacaa ggatgacgat gacaagggag gcggtggaag cggcgcaaca aacttctctc
     7021 tgctgaaaca agccggagat gtcgaagaga atcctggacc gatggtgagc aagggcgagg
     7081 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     7141 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     7201 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     7261 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     7321 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     7381 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     7441 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     7501 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     7561 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     7621 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     7681 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     7741 ccgccgggat cactctcggc atggacgagc tgtacaagtg accggtcatc atcaccatca
     7801 ccattgagtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     7861 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     7921 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     7981 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     8041 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcga taccgtcgac
     8101 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     8161 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct agggtgccta
     8221 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     8281 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     8341 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     8401 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag g
//