pET42b-ABE7.10

LOCUS       pET42b-ABE7.10               10570 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expresses ABE7.10-NLS with an N-terminal His Tag (His6) for 
            bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    pET42b-ABE7.10
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10570)
  AUTHORS   Liang P, Xie X, Zhi S, Sun H, Zhang X, Chen Y, Chen Y, Xiong Y, Ma 
            W, Liu D, Huang J, Songyang Z
  TITLE     Genome-wide profiling of adenine base editor specificity by 
            EndoV-seq.
  JOURNAL   Nat Commun. 2019 Jan 8;10(1):67. doi: 10.1038/s41467-018-07988-z.
  PUBMED    30622278
REFERENCE   2  (bases 1 to 10570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..10570
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    80..104
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             119..141
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             160..177
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1366..5466
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5479..5499
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5565..5583)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5579..5626
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      5663..6118
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5750..5769)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5960..5981
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6210..7025)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6933..6952
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7017..7036
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      7147..7735
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7636..7655
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7889..7906
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7921..8060
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7986..8005)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8146..8168
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8162..8353)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     YELYRSCLARFGDDGENL"
     CDS             complement(9162..10244)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNHASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQLLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESWQ"
     primer_bind     10206..10225
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(10245..10322)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     10528..10547
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
       61 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
      121 tgtttaactt taagaaggag atataccatg ggcagcagtc atcatcatca ccatcactcg
      181 gaggttgaat tctcccacga gtattggatg cggcacgctc ttacgttagc aaaacgcgcg
      241 tgggacgagc gtgaagtacc ggtaggcgcc gtgttagtgc ataataaccg ggtcattggt
      301 gaaggatgga atcggccgat cgggagacac gatccgacag cacatgctga gattatggct
      361 ttacggcaag gaggactggt tatgcagaac taccggttga ttgatgctac actgtacgta
      421 accttagaac catgtgtgat gtgtgctgga gccatgatac attcccgcat cggaagagtg
      481 gtttttgggg ctcgtgatgc aaaaactggc gccgccggaa gtcttatgga cgtgttacat
      541 catccaggca tgaatcatcg ggtcgagatt acagagggca ttttggcaga tgaatgtgct
      601 gcattgctta gtgatttctt ccgcatgcgg agacaggaaa tcaaagccca aaaaaaagct
      661 caaagtagta ctgatagtgg tggatccagt ggaggctcgt caggctctga aacgcctggc
      721 acatcagaat cggcaacgcc agagtcgtca ggaggttcct caggtggatc ttcggaggtc
      781 gagttttcac atgagtattg gatgcgtcat gccttgacgt tggcgaaacg ggcgcgcgat
      841 gagcgtgagg tgcccgtggg agcggtgttg gtactgaata accgggttat aggggaagga
      901 tggaaccggg ctattgggtt acacgaccca acggcgcacg ccgagataat ggcactgcgc
      961 caagggggct tagttatgca gaattatcgc cttatcgatg ctacactgta tgtaaccttt
     1021 gaaccctgcg taatgtgtgc gggggctatg atccactcga gaatagggcg cgtggtattc
     1081 ggcgtacgca acgctaaaac cggggctgcg ggctcgttga tggatgttct gcactacccc
     1141 ggaatgaatc acagagtaga gatcacggag ggaattttgg ccgacgaatg tgcagcttta
     1201 ctgtgctact tttttcggat gccgcggcaa gtcttcaacg cacagaagaa ggctcaatct
     1261 tccactgact caggtggctc gagtggtggg agtagcggat ctgagacgcc aggcacatca
     1321 gagagtgcaa cccccgagtc atcgggtggg agttccggcg gatctgataa gaaatactca
     1381 ataggcttag ctatcggcac aaatagcgtc ggatgggcgg tgatcactga tgaatataag
     1441 gttccgtcta aaaagttcaa ggttctggga aatacagacc gccacagtat caaaaaaaat
     1501 cttatagggg ctcttttatt tgacagtgga gagacagcgg aagcgactcg tctcaaacgg
     1561 acagctcgta gaaggtatac acgtcggaag aatcgtattt gttatctaca ggagattttt
     1621 tcaaatgaga tggcgaaagt agatgatagt ttctttcatc gacttgaaga gtcttttttg
     1681 gtggaagaag acaagaagca tgaacgtcat cctatttttg gaaatatagt agatgaagtt
     1741 gcttatcatg agaaatatcc aactatctat catctgcgaa aaaaattggt agattctact
     1801 gataaagcgg atttgcgctt aatctatttg gccttagcgc atatgattaa gtttcgtggt
     1861 cattttttga ttgagggaga tttaaatcct gataatagtg atgtggacaa actatttatc
     1921 cagttggtac aaacctacaa tcaattattt gaagaaaacc ctattaacgc aagtggagta
     1981 gatgctaaag cgattctttc tgcacgattg agtaaatcaa gacgattaga aaatctcatt
     2041 gctcagctcc ccggtgagaa gaaaaatggc ttatttggga atctcattgc tttgtcattg
     2101 ggtttgaccc ctaattttaa atcaaatttt gatttggcag aagatgctaa attacagctt
     2161 tcaaaagata cttacgatga tgatttagat aatttattgg cgcaaattgg agatcaatat
     2221 gctgatttgt ttttggcagc taagaattta tcagatgcta ttttactttc agatatccta
     2281 agagtaaata ctgaaataac taaggctccc ctatcagctt caatgattaa acgctacgat
     2341 gaacatcatc aagacttgac tcttttaaaa gctttagttc gacaacaact tccagaaaag
     2401 tataaagaaa tcttttttga tcaatcaaaa aacggatatg caggttatat tgatggggga
     2461 gctagccaag aagaatttta taaatttatc aaaccaattt tagaaaaaat ggatggtact
     2521 gaggaattat tggtgaaact aaatcgtgaa gatttgctgc gcaagcaacg gacctttgac
     2581 aacggctcta ttccccatca aattcacttg ggtgagctgc atgctatttt gagaagacaa
     2641 gaagactttt atccattttt aaaagacaat cgtgagaaga ttgaaaaaat cttgactttt
     2701 cgaattcctt attatgttgg tccattggcg cgtggcaata gtcgttttgc atggatgact
     2761 cggaagtctg aagaaacaat taccccatgg aattttgaag aagttgtcga taaaggtgct
     2821 tcagctcaat catttattga acgcatgaca aactttgata aaaatcttcc aaatgaaaaa
     2881 gtactaccaa aacatagttt gctttatgag tattttacgg tttataacga attgacaaag
     2941 gtcaaatatg ttactgaagg aatgcgaaaa ccagcatttc tttcaggtga acagaagaaa
     3001 gccattgttg atttactctt caaaacaaat cgaaaagtaa ccgttaagca attaaaagaa
     3061 gattatttca aaaaaataga atgttttgat agtgttgaaa tttcaggagt tgaagataga
     3121 tttaatgctt cattaggtac ctaccatgat ttgctaaaaa ttattaaaga taaagatttt
     3181 ttggataatg aagaaaatga agatatctta gaggatattg ttttaacatt gaccttattt
     3241 gaagataggg agatgattga ggaaagactt aaaacatatg ctcacctctt tgatgataag
     3301 gtgatgaaac agcttaaacg tcgccgttat actggttggg gacgtttgtc tcgaaaattg
     3361 attaatggta ttagggataa gcaatctggc aaaacaatat tagatttttt gaaatcagat
     3421 ggttttgcca atcgcaattt tatgcagctg atccatgatg atagtttgac atttaaagaa
     3481 gacattcaaa aagcacaagt gtctggacaa ggcgatagtt tacatgaaca tattgcaaat
     3541 ttagctggta gccctgctat taaaaaaggt attttacaga ctgtaaaagt tgttgatgaa
     3601 ttggtcaaag taatggggcg gcataagcca gaaaatatcg ttattgaaat ggcacgtgaa
     3661 aatcagacaa ctcaaaaggg ccagaaaaat tcgcgagagc gtatgaaacg aatcgaagaa
     3721 ggtatcaaag aattaggaag tcagattctt aaagagcatc ctgttgaaaa tactcaattg
     3781 caaaatgaaa agctctatct ctattatctc caaaatggaa gagacatgta tgtggaccaa
     3841 gaattagata ttaatcgttt aagtgattat gatgtcgatc acattgttcc acaaagtttc
     3901 cttaaagacg attcaataga caataaggtc ttaacgcgtt ctgataaaaa tcgtggtaaa
     3961 tcggataacg ttccaagtga agaagtagtc aaaaagatga aaaactattg gagacaactt
     4021 ctaaacgcca agttaatcac tcaacgtaag tttgataatt taacgaaagc tgaacgtgga
     4081 ggtttgagtg aacttgataa agctggtttt atcaaacgcc aattggttga aactcgccaa
     4141 atcactaagc atgtggcaca aattttggat agtcgcatga atactaaata cgatgaaaat
     4201 gataaactta ttcgagaggt taaagtgatt accttaaaat ctaaattagt ttctgacttc
     4261 cgaaaagatt tccaattcta taaagtacgt gagattaaca attaccatca tgcccatgat
     4321 gcgtatctaa atgccgtcgt tggaactgct ttgattaaga aatatccaaa acttgaatcg
     4381 gagtttgtct atggtgatta taaagtttat gatgttcgta aaatgattgc taagtctgag
     4441 caagaaatag gcaaagcaac cgcaaaatat ttcttttact ctaatatcat gaacttcttc
     4501 aaaacagaaa ttacacttgc aaatggagag attcgcaaac gccctctaat cgaaactaat
     4561 ggggaaactg gagaaattgt ctgggataaa gggcgagatt ttgccacagt gcgcaaagta
     4621 ttgtccatgc cccaagtcaa tattgtcaag aaaacagaag tacagacagg cggattctcc
     4681 aaggagtcaa ttttaccaaa aagaaattcg gacaagctta ttgctcgtaa aaaagactgg
     4741 gatccaaaaa aatatggtgg ttttgatagt ccaacggtag cttattcagt cctagttgtt
     4801 gctaaggtgg aaaaagggaa atcgaagaag ttaaaatccg ttaaagagtt actagggatc
     4861 acaattatgg aaagaagttc ctttgaaaaa aatccgattg actttttaga agctaaagga
     4921 tataaggaag ttaaaaaaga cttaatcatt aaactaccta aatatagtct ttttgagtta
     4981 gaaaacggtc gtaaacggat gctggctagt gccggagaat tacaaaaagg aaatgagctg
     5041 gctctgccaa gcaaatatgt gaatttttta tatttagcta gtcattatga aaagttgaag
     5101 ggtagtccag aagataacga acaaaaacaa ttgtttgtgg agcagcataa gcattattta
     5161 gatgagatta ttgagcaaat cagtgaattt tctaagcgtg ttattttagc agatgccaat
     5221 ttagataaag ttcttagtgc atataacaaa catagagaca aaccaatacg tgaacaagca
     5281 gaaaatatta ttcatttatt tacgttgacg aatcttggag ctcccgctgc ttttaaatat
     5341 tttgatacaa caattgatcg taaacgatat acgtctacaa aagaagtttt agatgccact
     5401 cttatccatc aatccatcac tggtctttat gaaacacgca ttgatttgag tcagctagga
     5461 ggtgactctg gcgggtctcc caagaagaag aggaaagtct aataattgat taatacctag
     5521 gctgctaaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact
     5581 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac
     5641 tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     5701 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     5761 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     5821 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     5881 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     5941 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccatatc
     6001 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     6061 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
     6121 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     6181 caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac
     6241 tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat
     6301 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg
     6361 attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta
     6421 tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc
     6481 atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca
     6541 tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg
     6601 ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca
     6661 tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg
     6721 gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc
     6781 ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg
     6841 gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat
     6901 cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa
     6961 tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg
     7021 ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa
     7081 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     7141 atcttcttga gatccttttt ttctgcgcgt aatctgctga ttgcaaacaa aaaaaccacc
     7201 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     7261 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     7321 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatca tgttaccagt
     7381 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     7441 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccaa gcttggagcg
     7501 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     7561 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     7621 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     7681 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     7741 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     7801 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     7861 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     7921 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca atggtgcact
     7981 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac
     8041 gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     8101 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     8161 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcataag
     8221 cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt
     8281 tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt
     8341 cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac
     8401 cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac
     8461 tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca
     8521 ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc
     8581 agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca
     8641 gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt
     8701 tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa
     8761 ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg
     8821 gggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag
     8881 tgacgaaggc ttgagcgagg gagtgcaaga ttccgaatac cgcaagcgac aggccgatca
     8941 tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct
     9001 gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc
     9061 gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgagatcccg
     9121 gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg ctcactgcca gctttccagt
     9181 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     9241 tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg agacgggcaa cagctgattg
     9301 cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt ttgccccagc
     9361 aggcgaaaat cctgtttgat ggtggttaac ggcgggatat aacatgagct gtcttcggta
     9421 tcgtcgtatc ccactaccga gatatccgca ccaacgcgca gcccggactc ggtaatggcg
     9481 cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg aacgatgccc
     9541 tcattcagca tttgcatggt ttgttgaaaa ccggacatgg cactccagtc gccttcccgt
     9601 tccgctatcg gctgaatttg attgcgagtg agatatttat gccagccagc cagacgcaga
     9661 cgcgccgaga cagaacttaa tgggcccgct aacagcgcga tttgctggtg acccaatgcg
     9721 accagatgct ccacgcccag tcgcgtaccg tcttcatggg agaaaataat actgttgatg
     9781 ggtgtctggt cagagacatc aagaaataac gccggaacat tagtgcaggc agcttccaca
     9841 gcaatggcat cctggtcatc cagcggatag ttaatgatca gcccactgac gcgttgcgcg
     9901 agaagattgt gcaccgccgc tttacaggct tcgacgccgc ttcgttctac catcgacacc
     9961 accacgctgg cacccagttg atcggcgcga gatttaatcg ccgcgacaat ttgcgacggc
    10021 gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca acaactgttt gcccgccagt
    10081 tgttgtgcca cgcggttggg aatgtaattc agctccgcca tcgccgcttc cactttttcc
    10141 cgcgttttcg cagaaacgtg gctggcatgg ttcaccacgc gggaaacggt ctgataagag
    10201 acaccggcat actctgcgac atcgtataac gttactggtt tcacattcac caccctgaat
    10261 tgactctctt ccgggcgcta tcatgccata ccgcgaaagg ttttgcgcca ttcgatggtg
    10321 tccgggatct cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag
    10381 gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa
    10441 cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc
    10501 aaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc
    10561 acctgtggcg
//