| 质粒名称: | pSL1637 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002457 |
| 产品用途: | Mammalian PhCMV-driven constitutive L7Ae-LOV2 expression vector (PhCMV- L7Ae-LOV2-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | PhCMV |
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | L7Ae-LOV2 |
| 物种来源: | synthetic |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Rationally designed light-inducible RNA-releasing protein for translational regulation and optogenetic control of gene therapies
NA
NA
|
pSL1637
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pSL1637载体质粒基本信息
pSL1637质粒图谱载体图谱和pSL1637载体序列质粒序列多克隆位点信息
pSL1637质粒载体简介
pSL1637质粒序列载体序列
LOCUS pSL1637 6204 bp DNA circular SYN 09-MAR-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6204)
AUTHORS Trial User
TITLE Direct Submission
JOURNAL Exported Aug 31, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6204
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 213..232
/label=oSCL771
misc_feature 232..819
/label=CMV
primer_bind 863..882
/label=T7
primer_bind 863..882
/label=T7
primer_bind 880..932
/label=oYF0021
primer_bind 880..900
/label=oSCL1162
primer_bind 886..932
/label=oSCL1786
primer_bind 903..926
/label=oSCl008
primer_bind 909..932
/label=DDL7AeF
/note="KpnI"
misc_feature 911..1268
/direction=RIGHT
/note="note=""Fragment extracted from PCR(pWH132.xdna) [363
nt] : (BamHI[6] / XbaI[373])"
primer_bind 912..932
/label=L7Ae FLP
primer_bind 1232..1242
/label=oSCL1162
primer_bind complement(1244..1269)
/label=DDL7AeR
/note="EcoRI"
primer_bind complement(1248..1268)
/label=L7Ae RLE
primer_bind complement(1250..1298)
/label=oSCL009
primer_bind complement(1250..1288)
/label=oYF0022
primer_bind 1255..1288
/label=oLZ048 NSP3-F
primer_bind 1255..1269
/label=oLZ048 NSP3-F
primer_bind complement(1269..1304)
/label=oSCL1785
misc_feature 1269..1303
/label=linker
primer_bind 1269..1298
/label=oSCL388
primer_bind 1269..1298
/label=oSCL388a
primer_bind 1269..1288
/label=oSCL1192
primer_bind 1269..1286
/label=oLZ147 NS3a F
primer_bind 1269..1285
/label=oYF0033
primer_bind 1278..1298
/label=oSCL327
primer_bind complement(1279..1304)
/label=oSCL1119
primer_bind complement(1279..1298)
/label=oSCL075
primer_bind 1284..1325
/label=oSCL1819
misc_feature 1305..1718
/label=LOV2
primer_bind 1305..1325
/label=oSCL1757
primer_bind complement(1697..1755)
/label=oSCL1994
primer_bind complement(1697..1741)
/label=oSCL2665
primer_bind complement(1697..1718)
/label=oSCL1758
terminator 1818..2045
/label=bGH PA
rep_origin 2091..2519
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2533..2862
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
CDS 2929..3723
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3897..4018
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4063..4066)
/label=M13-rev
misc_binding complement(4072..4094)
/label=LacO
promoter complement(4098..4128)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4143..4164
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(4452..5037)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5208..6068)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(6069..6173)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagg
901 aattcgccac catgtacgtg cgcttcgagg tgcccgagga catgcagaac gaggccctga
961 gcctgctgga aaaagtgcgc gagagcggca aagtgaagaa gggcaccaac gaaaccacca
1021 aggccgtgga acggggcctg gccaagctgg tgtatatcgc cgaggacgtg gacccccccg
1081 agattgtggc ccatctgccc ctgctgtgcg aagagaagaa cgtgccctac atctacgtga
1141 agtccaagaa cgacctgggc agagccgtgg gcatcgaggt gccatgtgcc tctgccgcca
1201 tcatcaacga gggcgagctg cggaaagaac tgggcagcct ggtggaaaag atcaagggcc
1261 tgcagaaggc cagcggaagt ggcggaggag gcgacgtcgg atccggtttg gaacgtatcg
1321 aaaagaattt tgtcatcacg gacccgcgtc tgcccgacaa tccgattatc ttcgcgtcag
1381 actctttctt acaactgact gagtatagta gagaggagat attggggcgt aactgtagat
1441 ttcttcaggg gccagaaact gatcgggcta ccgttcgcaa gatacgtgac gcaatagaca
1501 accagaccga ggtgacggtg cagctgatta actacacaaa gtctgggaag aagttctgga
1561 acctgtttca tttacaacct atgagggacc aaaaaggtga cgttcaatat ttcatcgggg
1621 ttcagttaga tgggactgag cacgtgagag atgcagcaga aagagagggt gtaatgctta
1681 ttaaaaaaac agccgagaat atcgacgaag ccgctggtta aggatccact agtccagtgt
1741 ggtggaattc tgcagatatc cagcacagtg gcggccgctc gagtctagag ggcccgttta
1801 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
1861 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
1921 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
1981 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
2041 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
2101 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
2161 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
2221 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
2281 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
2341 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
2401 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
2461 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
2521 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
2581 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
2641 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
2701 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
2761 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
2821 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
2881 atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
2941 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
3001 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
3061 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
3121 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
3181 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
3241 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
3301 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
3361 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
3421 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc
3481 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
3541 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
3601 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
3661 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
3721 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
3781 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
3841 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
3901 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
3961 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
4021 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatgtgtg aaattgttat
4081 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
4141 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
4201 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
4261 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
4321 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
4381 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
4441 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
4501 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
4561 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
4621 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
4681 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
4741 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
4801 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
4861 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
4921 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
4981 ggtagcggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
5041 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
5101 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
5161 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
5221 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
5281 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
5341 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
5401 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
5461 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
5521 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
5581 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
5641 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
5701 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
5761 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
5821 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
5881 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
5941 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
6001 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
6061 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
6121 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
6181 ccccgaaaag tgccacctga cgtc
//
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