pAAVLINKv1-pCALM1-Cre-FBN1-C-FLAG

LOCUS       pAAVLINKv1-pCALM1-Cre-FBN1-C-FLAG              7117 bp DNA     circular SYN 24-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7117)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 8, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7117
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     promoter        145..265
                     /label=pCALM1
     CDS             277..1308
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     intron          1309..1349
                     /label=Splicing Donor
     protein_bind    1356..1389
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1390..1481
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1482..3830
                     /codon_start=1
                     /label=FBN1
                     /translation="GEGWGDPCELCPTEPDEAFRQICPYGSGIIVGPDDSAVDMDECKE
                     PDVCKHGQCINTDGSYRCECPFGYILAGNECVDTDECSVGNPCGNGTCKNVIGGFECTC
                     EEGFEPGPMMTCEDINECAQNPLLCAFRCVNTYGSYECKCPVGYVLREDRRMCKDEDEC
                     EEGKHDCTEKQMECKNLIGTYMCICGPGYQRRPDGEGCVDENECQTKPGICENGRCLNT
                     RGSYTCECNDGFTASPNQDECLDNREGYCFTEVLQNMCQIGSSNRNPVTKSECCCDGGR
                     GWGPHCEICPFQGTVAFKKLCPHGRGFMTNGADIDECKVIHDVCRNGECVNDRGSYHCI
                     CKTGYTPDITGTSCVDLNECNQAPKPCNFICKNTEGSYQCSCPKGYILQEDGRSCKDLD
                     ECATKQHNCQFLCVNTIGGFTCKCPPGFTQHHTSCIDNNECTSDINLCGSKGICQNTPG
                     SFTCECQRGFSLDQTGSSCEDVDECEGNHRCQHGCQNIIGGYRCSCPQGYLQHYQWNQC
                     VDENECLSAHICGGASCHNTLGSYKCMCPAGFQYEQFSGGCQDINECGSAQAPCSYGCS
                     NTEGGYLCGCPPGYFRIGQGHCVSGMGMGRGNPEPPVSGEMDDNSLSPEACYECKINGY
                     PKRGRKRRSTNETDASNIEDQSETEANVSLASWDVEKTAIFAFNISHVSNKVRILELLP
                     ALTTLTNHNRYLIESGNEDGFFKINQKEGISYLHFTKKKPVAGTYSLQISSTPLYKKKE
                     LNQLEDKYDKDYLSGELGDNLKMKIQVLLH"
     misc_feature    1795..1800
                     /label=SDS
     misc_feature    3076..3081
                     /label=SDS
     CDS             3837..3902
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3906..4337
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    4050..4055
                     /label=SDS
     repeat_region   4380..4520
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4380..4509
                     /label=AAV2 ITR
     rep_origin      4595..5050
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5332..5436
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5437..6297
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6468..7056
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
      181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
      241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
      301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
      361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
      421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
      481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
      541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
      601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
      661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
      721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
      781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
      841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
      901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
      961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
     1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
     1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
     1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
     1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
     1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
     1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
     1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
     1441 tattggtctt actgacatcc actttgcctt tctctccaca gggagaaggc tggggagacc
     1501 cctgcgagct ctgccccacg gaacctgatg aggccttccg ccagatatgt ccttatggaa
     1561 gtgggatcat cgtgggacct gatgattcag cagttgatat ggacgaatgc aaagaacccg
     1621 atgtctgtaa acatggacag tgcatcaata cagatggttc ctatcgctgc gagtgtccct
     1681 ttggttatat tctagcaggg aatgaatgtg tagatactga tgaatgttct gttggcaatc
     1741 cttgtggaaa tggaacctgc aagaatgtga ttggaggttt tgaatgcacc tgcgaggagg
     1801 gatttgagcc cggtccaatg atgacatgtg aagatataaa tgaatgtgcc cagaatcctc
     1861 tgctctgtgc cttccgatgt gtgaacactt atgggtcata tgaatgcaaa tgtcccgtgg
     1921 gatatgtgct cagagaagac cgtaggatgt gcaaagatga ggatgagtgt gaagagggaa
     1981 aacatgactg tactgaaaaa caaatggaat gcaagaacct cattggcaca tatatgtgca
     2041 tctgtggacc cgggtatcag cggagacctg atggagaagg ctgtgtagat gagaatgaat
     2101 gtcagacgaa gccagggatc tgtgagaatg ggcgctgcct caacacccgt gggagctaca
     2161 cctgtgagtg taatgatggg tttaccgcca gccccaacca ggacgagtgc cttgacaatc
     2221 gggaagggta ctgcttcaca gaggtgctac aaaacatgtg tcagatcggc tccagcaaca
     2281 ggaaccccgt caccaaatcg gaatgctgct gtgacggagg gagaggctgg ggtccccact
     2341 gtgagatctg ccctttccag gggactgtgg ctttcaagaa actctgtccc catggccgag
     2401 gattcatgac caatggagca gatatcgatg aatgcaaggt tattcacgat gtttgccgaa
     2461 atggggaatg tgtcaatgac agaggatcat atcattgcat ttgtaaaact gggtacactc
     2521 cagatataac tgggacttcc tgtgtagatc tgaacgagtg caaccaggct cccaaaccct
     2581 gcaattttat ctgcaaaaac acagaaggga gttaccagtg ttcatgcccg aaaggctaca
     2641 ttctgcaaga ggatggaagg agctgcaaag atcttgatga gtgtgcaacc aagcaacaca
     2701 actgccagtt cctatgtgtt aacaccattg gcggcttcac atgcaaatgt cctcccggat
     2761 ttacccaaca ccatacgtcc tgcattgata acaatgaatg cacctctgac atcaatctgt
     2821 gcgggtctaa gggcatttgc cagaacactc ctggaagctt cacctgtgaa tgccagcggg
     2881 gattctcact tgatcagacc ggctccagct gtgaagacgt ggacgagtgt gagggtaacc
     2941 accgctgcca gcatggctgc cagaacatca ttgggggcta caggtgcagc tgcccccagg
     3001 gctacctcca gcactaccag tggaaccagt gtgttgatga aaacgaatgc ctcagcgctc
     3061 acatctgcgg aggagcctcc tgtcacaaca ccctggggag ctacaagtgc atgtgtcccg
     3121 ccggcttcca gtatgaacag ttcagtggag gatgccaaga catcaatgaa tgtggctctg
     3181 cgcaggcccc ctgcagctat ggctgttcca ataccgaggg cggttacctg tgtggctgtc
     3241 cacctggtta cttccgcata ggccaagggc actgtgtttc tggaatgggc atgggccgag
     3301 gaaacccaga gccacctgtc agtggtgaaa tggatgacaa ttcactctcc ccagaggctt
     3361 gttacgagtg taagatcaat ggctacccca aacggggcag gaaacggaga agcacaaacg
     3421 aaactgatgc ctccaatatc gaggatcagt ctgagacaga agccaatgtg agtcttgcaa
     3481 gttgggatgt tgagaagaca gccatctttg ctttcaatat ttcccacgtc agtaacaagg
     3541 ttcgaatcct agaactcctt ccagctctta caactctgac gaatcacaac agatacttga
     3601 tcgaatctgg aaatgaagat ggcttcttta aaatcaacca aaaggaaggg atcagctacc
     3661 tccacttcac aaagaagaag ccagtggctg gaacctattc attacaaatc agtagtactc
     3721 cactttataa aaagaaagaa cttaaccaac tagaagacaa atatgacaaa gactacctca
     3781 gtggtgaact gggtgataat ctgaagatga aaatccaggt tttgcttcat tgtacagact
     3841 acaaagacca tgacggtgat tataaagatc atgacatcga ctacaaggat gacgatgaca
     3901 agtaactcga gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     3961 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     4021 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tagttcttgc
     4081 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     4141 cactgacaat tccgtggtgt ttatttgtga aatttgtgat gctattgctt tatttgtaac
     4201 catctagctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     4261 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggaga
     4321 tgtgggaggt tttttaaaga tctggagacc ggtgtggcac gtgcggaccg agcggccgca
     4381 ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc
     4441 cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg
     4501 agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     4561 tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc
     4621 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cttagcgccc
     4681 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     4741 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     4801 aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     4861 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     4921 ctcaactcta tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggtctat
     4981 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     5041 tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     5101 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     5161 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     5221 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     5281 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     5341 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     5401 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     5461 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     5521 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     5581 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     5641 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     5701 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     5761 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     5821 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     5881 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     5941 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     6001 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     6061 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     6121 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     6181 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     6241 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     6301 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     6361 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     6421 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     6481 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     6541 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     6601 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6661 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6721 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     6781 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     6841 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     6901 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     6961 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     7021 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     7081 cggttcctgg ccttttgctg gccttttgct cacatgt
//