| 质粒名称: | pAAVLINK-EF1alpha-HA-SRCAP-A |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001936 |
| 产品用途: | 5' AAVLINK plasmid for SRCAP expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1alpha |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | SRCAP |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1alpha-HA-SRCAP-A
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1alpha-HA-SRCAP-A载体质粒基本信息
pAAVLINK-EF1alpha-HA-SRCAP-A质粒图谱载体图谱和pAAVLINK-EF1alpha-HA-SRCAP-A载体序列质粒序列多克隆位点信息
pAAVLINK-EF1alpha-HA-SRCAP-A质粒载体简介
pAAVLINK-EF1alpha-HA-SRCAP-A质粒序列载体序列
LOCUS pAAVLINK-EF1alpha-HA-SRCAP-A 6690 bp DNA circular SYN 10-AUG-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6690)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 16, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6690
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
/note="color: #ffe4c4"
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..3830
/codon_start=1
/gene="SRCAP"
/gene_synonym="DEHMBA; DOMO1; EAF1; FLHS; SWR1"
/product="helicase SRCAP"
/label=SRCAP
/note="Snf2-related CBP activator protein;Swi2/Snf2-related
ATPase homolog, domino homolog 1; domino homolog 2"
/db_xref="GI:146219843"
/db_xref="CCDS:CCDS10689.2"
/db_xref="GeneID:10847"
/db_xref="HGNC:HGNC:16974"
/db_xref="MIM:611421"
/protein_id="NP_006653.2"
/translation="QSSPSPAHPQLPVLQTQMVSDGMTGSNPVSPASSSSPASSGAGGI
SPQHIAQDSSLDGPPGPPDGATVPLEGFSLSQAADLANKGPKWEKSHAEIAEQAKHEAE
IETRIAELRKEGFWSLKRLPKVPEPPRPKGHWDYLCEEMQWLSADFAQERRWKRGVARK
VVRMVIRHHEEQRQKEERARREEQAKLRRIASTMAKDVRQFWSNVEKVVQFKQQSRLEE
KRKKALDLHLDFIVGQTEKYSDLLSQSLNQPLTSSKAGSSPCLGSSSAASSPPPPASRL
DDEDGDFQPQEDEEEDDEETIEVEEQQEGNDAEAQRREIELLRREGELPLEELLRSLPP
QLLEGPSSPSQTPSSHDSDTRDGPEEGAEEEPPQVLEIKPPPSAVTQRNKQPWHPDEDD
EEFTANEEEAEDEEDTIAAEEQLEGEVDHAMELSELAREGELSMEELLQQYAGAYAPGS
GSSEDEDEDEVDANSSDCEPEGPVEAEEPPQEDSSSQSDSVEDRSEDEEDEHSEEEETS
GSSASEESESEESEDAQSQSQADEEEEDDDFGVEYLLARDEEQSEADAGSGPPTPGPTT
LGPKKEITDIAAAAESLQPKGYTLATTQVKTPIPLLLRGQLREYQHIGLDWLVTMYEKK
LNGILADEMGLGKTIQTISLLAHLACEKGNWGPHLIIVPTSVMLNWEMELKRWCPSFKI
LTYYGAQKERKLKRQGWTKPNAFHVCITSYKLVLQDHQAFRRKNWRYLILDEAQNIKNF
KSQRWQSLLNFNSQRRLLLTGTPLQNSLMELWSLMHFLMPHVFQSHREFKEWFSNPLTG
MIEGSQEYNEGLVKRLHKVLRPFLLRRVKVDVEKQMPKKYEHVIRCRLSKRQRCLYDDF
MAQTTTKETLATGHFMSVINILMQLRKVCNHPNLFDPRPVTSPFITPGICFSTASLVLR
ATDVHPLQRIDMGRFDLIGLEGRVSRYEADTFLPRHRLSRRVLLEVATAPDPPPRPKPV
KMKVNRMLQPVPKQEGRTVVVVNNPRAPLGPVPVRPPP"
misc_feature 1089..1094
/label=SDS
misc_feature 2207..2212
/label=SDS
misc_feature 2214..2219
/label=SDS
misc_feature 2283..2288
/label=SDS
misc_feature 2373..2378
/label=SDS
misc_feature 2453..2458
/label=SDS
misc_feature 3821..3826
/label=SDS
intron 3831..3871
/label=Splicing Donor
misc_feature 3875..3908
/label=loxP-JT1571
repeat_region 3953..4093
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3953..4082
/label=AAV2 ITR
repeat_region 3954..4094
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 4168..4623
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4905..5009
/gene="bla"
/label=AmpR promoter
CDS 5010..5870
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6041..6629
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac tgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctca gagcagcccc tcccctgctc accctcagct
781 cccagtccta cagacacaga tggtgtcgga cggcatgaca ggcagcaatc ctgtgtcccc
841 tgcctcatcc agttccccag cctctagtgg ggcaggcggc atctccccgc agcacatagc
901 tcaagattcc tcactggatg gacctccagg ccccccagat ggtgccacag tgcccctgga
961 ggggttcagc ttatcccagg ctgctgacct ggctaacaag ggcccgaagt gggagaagag
1021 ccatgccgaa attgcagaac aggccaagca tgaggccgag atcgagactc ggattgctga
1081 gctgcggaag gagggtttct ggtcactgaa gaggctgcct aaggtgccag agccccctcg
1141 ccccaaaggt cactgggact atttgtgcga agagatgcag tggctctctg ctgactttgc
1201 tcaggagcgc cgttggaaac ggggtgtggc ccggaaggtg gtgcgcatgg tgatccggca
1261 ccacgaggag cagcggcaga aagaggaacg ggcccggagg gaggagcagg ccaagctgcg
1321 tcgaattgct tccaccatgg ccaaggatgt caggcagttc tggagcaatg tggagaaggt
1381 ggtgcaattc aagcaacagt cccggcttga ggaaaagcgc aaaaaagccc tggacctgca
1441 tttggacttc attgtggggc aaactgaaaa gtactcggac cttctgtctc agagcctcaa
1501 ccagccatta acctccagca aagcaggctc ttccccttgc ctcggctctt cctcagctgc
1561 ctccagtcct ccaccccctg cttctcgcct ggatgatgaa gatggggact ttcaacccca
1621 agaggatgag gaagaggatg atgaggaaac gattgaagtt gaagaacaac aggaaggcaa
1681 tgatgcagag gcccagaggc gtgagattga gctgcttcgc cgtgagggag aattgccact
1741 ggaagagctg ctccgttccc ttccccctca gctgttggaa gggccttcca gcccctctca
1801 aaccccctca tctcatgata gtgacacccg agatgggcct gaagaaggtg ctgaagaaga
1861 gccccctcag gtgttggaga taaagccccc accctctgct gtcacacagc gcaacaaaca
1921 gccttggcat ccagatgaag atgatgaaga gtttactgcc aacgaagagg aagcggagga
1981 tgaagaggat actatagcag ctgaggaaca gttggaaggg gaggtggatc atgccatgga
2041 gctgagcgag ttggctcgag aaggtgagct ttccatggag gagctattgc agcagtatgc
2101 aggagcctat gccccaggct ctgggagcag tgaagatgag gatgaagatg aggttgatgc
2161 taatagctct gactgtgaac cagaggggcc cgtggaagcg gaagagcctc ctcaggagga
2221 tagtagcagt cagtcagact ctgtggagga ccggagtgag gatgaggaag atgaacattc
2281 agaggaggaa gaaacaagtg gaagttcagc atcagaggaa tctgagtctg aagagtctga
2341 ggatgcccaa tcacagagcc aagcagatga agaggaggaa gatgatgatt ttggggtgga
2401 gtacttgctt gccagggatg aagagcagag tgaggcagat gcaggcagtg ggcctcctac
2461 tccagggccc actactctag gtccaaagaa agaaattact gacattgctg cagcagctga
2521 aagtctccag cccaagggtt acacgctggc cacgacccag gtaaagacgc ccattcccct
2581 gcttctgcgg ggccagctcc gggagtacca gcacattggg ctagactggc tggttaccat
2641 gtatgagaag aagcttaatg gcattcttgc tgatgagatg gggcttggga agaccatcca
2701 gaccatctct ctgcttgccc acttggcttg tgagaaaggt aactggggtc cccatttaat
2761 cattgttccc accagcgtga tgttgaactg ggagatggag ttgaaacgtt ggtgccccag
2821 ctttaaaatc ctcacttact atggagccca gaaagagagg aagctcaagc ggcagggctg
2881 gaccaagccc aatgcctttc atgtgtgtat cacatcttac aagctggtgc tgcaggacca
2941 ccaggccttc cgtcgcaaga actggcgcta tctcattctg gatgaggcgc agaacatcaa
3001 gaacttcaag tcacagcgct ggcagtcact cctcaacttc aacagccaga gacgcctgct
3061 cctgacagga actcccttgc agaacagcct catggagctg tggtccttga tgcacttttt
3121 gatgccccat gtcttccagt ctcatcgcga gttcaaggag tggttctcta atcccctaac
3181 tggcatgatt gagggcagcc aagagtataa tgaaggtcta gtcaaacgcc tccacaaggt
3241 tttgaggcct tttttactgc gccgagttaa ggtggatgtt gagaagcaga tgcccaaaaa
3301 gtacgagcat gttatccgct gcaggctctc caagcgtcaa cgctgtctct atgatgactt
3361 catggcacag accacaacta aggagacact agccacaggc catttcatga gcgtcatcaa
3421 cattttgatg cagctgagaa aagtttgcaa tcatccaaat ctgttcgacc ctcgaccggt
3481 tacctcccct ttcatcaccc caggcatctg cttcagcacc gcctctctgg tgctaagggc
3541 cacggatgtc catcccctcc agcggataga catgggtcga tttgacctta ttggcctgga
3601 aggtcgtgtc tctcgatatg aggcagacac atttctgccc cggcaccgcc tctctcgccg
3661 ggtactgtta gaagtggcta ctgctcctga ccccccaccc cggcccaagc cagtcaagat
3721 gaaggtcaac aggatgctgc agccagtacc taagcaagaa ggccggacag tggtggtggt
3781 gaacaaccca cgggcgcccc tgggccctgt cccagttcga cctcctccag gtaagtatca
3841 aggttacaag acaggtttaa ggagaccaat aagctattat tcgtatagca tacattatac
3901 gaagttatta agttatggag accggtgtgg cacgtgcgga ccgagcggcc gcaggaaccc
3961 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga
4021 ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc
4081 agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
4141 caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
4201 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccttagcg cccgctcctt
4261 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
4321 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
4381 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
4441 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaact
4501 ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggtc tattggttaa
4561 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
4621 ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
4681 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
4741 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
4801 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
4861 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
4921 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
4981 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
5041 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
5101 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
5161 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
5221 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
5281 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
5341 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
5401 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
5461 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
5521 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
5581 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
5641 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
5701 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
5761 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
5821 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
5881 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
5941 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
6001 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
6061 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
6121 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
6181 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
6241 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
6301 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
6361 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
6421 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
6481 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
6541 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
6601 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
6661 tggccttttg ctggcctttt gctcacatgt
//
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pAAVLINK-EF1alpha-HA-SRCAP-A质粒载体应用举例
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