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pTagRFP-tubulin载体质粒基本信息
pTagRFP-tubulin质粒图谱载体图谱和pTagRFP-tubulin载体序列质粒序列多克隆位点信息
pTagRFP-tubulin质粒载体简介
pTagRFP-tubulin质粒序列载体序列
LOCUS pTagRFP-tubulin 6039 bp DNA circular SYN 12-JUL-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6039)
AUTHORS Biofeng
TITLE Direct Submission
JOURNAL Exported Jul 12, 2023 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6039
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 61..364
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
regulatory 607..616
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 613..1323
/codon_start=1
/product="monomeric derivative of red fluorescent protein
from Entacmaea quadricolor (Merzlyak et al., 2007)"
/label=TagRFP
/note="mammalian codon-optimized"
/translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
ARYCDLPSKLGHKLN"
polyA_signal 2827..2948
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(2955..3410)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3437..3541
/gene="bla"
/label=AmpR promoter
promoter 3543..3900
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3751..3886
/label=SV40 ori
/note="SV40 origin of replication"
CDS 3935..4729
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 4961..5008
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 5337..5925
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggtcgcca ccatggtgtc taagggcgaa gagctgatta aggagaacat gcacatgaag
661 ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga gggcgaaggc
721 aagccctacg agggcaccca gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc
781 ttcgccttcg acatcctggc taccagcttc atgtacggca gcagaacctt catcaaccac
841 acccagggca tccccgactt ctttaagcag tccttccctg agggcttcac atgggagaga
901 gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag cctccaggac
961 ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa cggccctgtg
1021 atgcagaaga aaacactcgg ctgggaggcc aacaccgaga tgctgtaccc cgctgacggc
1081 ggcctggaag gcagaagcga catggccctg aagctcgtgg gcgggggcca cctgatctgc
1141 aacttcaaga ccacatacag atccaagaaa cccgctaaga acctcaagat gcccggcgtc
1201 tactatgtgg accacagact ggaaagaatc aaggaggccg acaaagagac ctacgtcgag
1261 cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg gcacaaactt
1321 aattccggac tcagatctcg agtgcgtgag tgcatctcca tccacgttgg ccaggctggt
1381 gtccagattg gcaatgcctg ctgggagctc tactgcctgg aacacggcat ccagcccgat
1441 ggccagatgc caagtgacaa gaccattggg ggaggagatg actccttcaa caccttcttc
1501 agtgagacgg gcgctggcaa gcacgtgccc cgggctgtgt ttgtagactt ggaacccaca
1561 gtcattgatg aagttcgcac tggcacctac cgccagctct tccaccctga gcagctcatc
1621 acaggcaagg aagatgctgc caataactat gcccgagggc actacaccat tggcaaggag
1681 atcattgacc ttgtgttgga ccgaattcgc aagctggctg accagtgcac cggtcttcag
1741 ggcttcttgg ttttccacag ctttggtggg ggaactggtt ctgggttcac ctccctgctc
1801 atggaacgtc tctcagttga ttatggcaag aagtccaagc tggagttctc catttaccca
1861 gcaccccagg tttccacagc tgtagttgag ccctacaact ccatcctcac cacccacacc
1921 accctggagc actctgattg tgccttcatg gtagacaatg aggccatcta tgacatctgt
1981 cgtagaaacc tcgatatcga gcgcccaacc tacactaacc ttaaccgcct tattagccag
2041 attgtgtcct ccatcactgc ttccctgaga tttgatggag ccctgaatgt tgacctgaca
2101 gaattccaga ccaacctggt gccctacccc cgcatccact tccctctggc cacatatgcc
2161 cctgtcatct ctgctgagaa agcctaccat gaacagcttt ctgtagcaga gatcaccaat
2221 gcttgctttg agccagccaa ccagatggtg aaatgtgacc ctcgccatgg taaatacatg
2281 gcttgctgcc tgttgtaccg tggtgacgtg gttcccaaag atgtcaatgc tgccattgcc
2341 accatcaaaa ccaagcgcag catccagttt gtggattggt gccccactgg cttcaaggtt
2401 ggcatcaact accagcctcc cactgtggtg cctggtggag acctggccaa ggtacagaga
2461 gctgtgtgca tgctgagcaa caccacagcc attgctgagg cctgggctcg cctggaccac
2521 aagtttgacc tgatgtatgc caagcgtgcc tttgttcact ggtacgtggg tgaggggatg
2581 gaggaaggcg agttttcaga ggcccgtgaa gatatggctg cccttgagaa ggattatgag
2641 gaggttggtg tggattctgt tgaaggagag ggtgaggaag aaggagagga atactaagga
2701 tccaccggat ctagataact gatcataatc agccatacca catttgtaga ggttttactt
2761 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
2821 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
2881 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
2941 gtatcttaac gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
3001 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
3061 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
3121 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
3181 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
3241 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
3301 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
3361 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac
3421 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
3481 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
3541 tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
3601 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
3661 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
3721 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
3781 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
3841 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
3901 gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
3961 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
4021 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
4081 caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg
4141 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
4201 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
4261 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
4321 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
4381 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
4441 actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg
4501 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
4561 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
4621 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
4681 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
4741 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
4801 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
4861 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa
4921 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat
4981 aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac
5041 tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc
5101 ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca
5161 ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt
5221 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
5281 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
5341 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
5401 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
5461 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
5521 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
5581 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
5641 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
5701 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
5761 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
5821 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
5881 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
5941 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
6001 tcccctgatt ctgtggataa ccgtattacc gccatgcat
//
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