pDisplay-GRAB_HTR6-1.0

LOCUS      pDisplay-GRAB_HTR6-1.0             8818 bp DNA     circular SYN 21-APR-2023
DEFINITION  Expresses the 5-HT sensor GRAB_HTR6-1.0 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8818)
  TITLE     Yulong Li lab sensors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 29, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
FEATURES             Location/Qualifiers
     source          1..8818
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        10..389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..593
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     544..564
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     638..657
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        638..656
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     sig_peptide     737..799
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTGD"
     protein_bind    800..824
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             824..3016
                     /codon_start=1
                     /label=GRAB_HTR6-1.0
                     /translation="TTGVPEPGPTANSTPAWGAGPPSAPGGSGWVAAALCVVIALTAAA
                     NSLLIALICTQPALRNTSNFFLVSLFTSDLMVGLVVMPPAMLNALYGRWVLARGLCLLW
                     TAFDVMCCSASILNLCLISLDRYLLILSPLRYKLRMTPLRALALVLGAWSLAALASFLP
                     LLLGWHELGHARPPVPGQCRLLASLPFVLVASGLTFFLPSGAICFTYCRILLAARKQAV
                     QVASLTTGMASQRGPDAVAAPPGGTERRPNGLGPERSAGPGGAEAEPLPTQLNGAPGEP
                     APAGPRDTDALDLEEGGNVYIKADKQKNGIKANFHIRHNIEDGGVQLAYHYQQNTPIGD
                     GPVLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVRKG
                     EELFTGVVPILVELDGDVNGHKFSVSGEGEGDATEGKLTLKFICTTGKLPVPWPTLVTT
                     LTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNR
                     IELKGIDFKEDGNILGHKLEYNTGAAARWHSRKALKASLSLGILLGMFFVTWLPFFVAN
                     IVQAVCDCISPGLFDVLTWLGYCNSTMNPIIYPLFMRDFKRALGRFLPCPRCPRERQAS
                     LASPSLRTSHSGPRPGLSLQQVLPLPLPPDSDSDSDAGSGGSSGLRLTAQLLLPGEATQ
                     DPPLPTRAAAAVNFFNIDPAEPELRPHPLGIPTIRLA"
     primer_bind     1177..1196
                     /label=WKG124-F1
     primer_bind     1735..1760
                     /label=WKG124-F3
     primer_bind     1924..1945
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2051..2072)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2312..2331)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2833..2857
                     /label=WKG124-F2
     misc_feature    3017..3602
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3184..3201)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3410..3429
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3603..4310
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3748..3766)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4016..4035
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4368..4397
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4401..4547
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     primer_bind     complement(4569..4586)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4575..4799
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(4931..5519)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5011..5030)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5848..5895
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     5920..5939
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(6127..6921)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTYGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6238..6257)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6848..6867
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     complement(7020..7039)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     7266..7286
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(7369..8229)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7992..8011
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8230..8334)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8361..8816
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8448..8467)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8658..8679
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccctgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
      601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
      661 cccaagcttg gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgg
      721 cttggggata tccaccatgg agacagacac actcctgcta tgggtactgc tgctctgggt
      781 tccaggttcc actggtgaca caagtttgta caaaaaagtt ggcaccaccg gtgtgcccga
      841 gccgggccca actgctaaca gcaccccagc ttggggggct ggtcctccaa gcgccccagg
      901 tggcagtggt tgggttgctg cagcattgtg cgtggttatc gccctgacag ctgccgcaaa
      961 cagcctgctc attgctctta tttgtaccca acccgcactc cggaatacgt ctaatttttt
     1021 cctggtctca ctgttcacga gcgaccttat ggtcgggctc gtagtcatgc ctcctgctat
     1081 gttgaacgcc ctgtatggga gatgggtcct ggcaaggggt ctttgccttc tgtggaccgc
     1141 atttgacgtc atgtgctgta gtgccagcat cttgaacctc tgtttgatta gtctggatag
     1201 atatctgctg attctgagcc cacttagata caagctgcgg atgactccgc tccgggccct
     1261 cgctttggtg ctgggcgcct ggtctctggc agcactggct tcattcttgc ctttgctgct
     1321 gggctggcac gaattggggc acgcaaggcc gccagttcca ggccagtgta ggctgcttgc
     1381 ctccctgccc ttcgtgttgg tggcttccgg cctcacattc tttctgccta gtggtgccat
     1441 ctgcttcact tactgccgga tacttctggc agctagaaaa caagccgttc aggttgcctc
     1501 cctcaccacc ggcatggcaa gccagcgggg tccggacgcc gtcgccgcgc cgccgggggg
     1561 caccgagcgc aggcccaacg gtctgggccc cgagcgcagc gcgggcccgg ggggcgcaga
     1621 ggccgaaccg ctgcccaccc agctcaacgg cgcccctggc gagcccgcgc cggccgggcc
     1681 gcgcgacacc gacgcgctgg acctggagga gggaggaaac gtctatatca aggccgacaa
     1741 gcagaagaac ggcatcaagg cgaacttcca catccgccac aacatcgagg acggcggcgt
     1801 gcagctcgcc taccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     1861 cgacaaccac tacctgagcg tgcagtccaa actttcgaaa gaccccaacg agaagcgcga
     1921 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     1981 gtacaagggc ggtaccggag ggagcatggt gagaaagggc gaggagctgt tcaccggggt
     2041 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     2101 cgagggtgag ggcgatgcca ccgagggcaa gctgaccctg aagttcatct gcaccaccgg
     2161 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
     2221 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     2281 ctacatccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     2341 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     2401 ggaggacggc aacatcctgg ggcacaagct ggagtacaac accggagcag cagcacgctg
     2461 gcactcaaga aaggcgctga aagcttcatt gagcttgggc atcttgctgg gcatgttctt
     2521 cgtaacatgg ctcccatttt ttgtggccaa tattgtccag gcggtgtgcg actgcatctc
     2581 accaggcctg ttcgatgtgc tcacttggct ggggtattgc aattccacta tgaaccccat
     2641 catatatccc ctgttcatga gagatttcaa gcgagcactc ggaaggtttc tgccatgccc
     2701 acggtgccca cgcgaaaggc aagcaagtct ggcctccccc tcactccgga cctcccattc
     2761 aggacccaga ccaggacttt cactccagca agttctgccc ctgccacttc ctccagattc
     2821 agactctgac tccgatgcgg gttcaggtgg ctctagcgga ctccggctta cggcccagct
     2881 tttgcttccc ggggaagcaa ctcaggaccc accattgcct actcgggctg ccgccgccgt
     2941 caatttcttc aacattgatc ctgcagagcc agaactcaga ccgcatcctc tgggtatacc
     3001 aaccattagg ctagcgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct
     3061 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg
     3121 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt
     3181 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg
     3241 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac
     3301 ctggcgacag gtgcctctgc ggccaacgcc acgtgtataa gatacacctg caaaggcggc
     3361 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     3421 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     3481 tctggggcct cggtacacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
     3541 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacaa
     3601 ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca
     3661 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
     3721 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc
     3781 ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc
     3841 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc
     3901 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg
     3961 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg
     4021 taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg
     4081 gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg
     4141 ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg
     4201 tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     4261 aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag gatggtaaaa
     4321 agaagaaaaa gaagtcaaag acaaagtgtg taattatgta agtcgacgaa caaaaactca
     4381 tctcagaaga ggatctgaat gctgtgggcc aggacacgca ggaggtcatc gtggtgccac
     4441 actccttgcc ctttaaggtg gtggtgatct cagccatcct ggccctggtg gtgctcacca
     4501 tcatctccct tatcatcctc atcatgcttt ggcagaagaa gccacgttag gcggccgctc
     4561 gagatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     4621 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     4681 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     4741 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     4801 ttctgaggcg gaaagaacca gtggcggtaa tacggttatc cacagaatcg ggggataacg
     4861 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     4921 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     4981 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     5041 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     5101 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     5161 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     5221 tatccggtaa ctatcgtctt gagtccaacc cggtaagacg cgacttatcg ccactggcag
     5281 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     5341 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     5401 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     5461 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     5521 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     5581 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     5641 gaagttttaa atcaatctaa agtatatatg agtaacctga ggctatggca gggcctgccg
     5701 ccccgacgtt ggctgcgagc cctgggcctt cacccgaact tggggggtgg ggtggggaaa
     5761 aggaagaaac gcgggcgtat tggccccaat ggggtctcgg tggggtatcg acagagtgcc
     5821 agccctggga ccgaaccccg cgtttatgaa caaacgaccc aacaccgtgc gttttattct
     5881 gtctttttat tgccgtcata gcgcgggttc cttccggtat tgtctccttc cgtgtttcag
     5941 ttagcctccc cctagggtgg gcgaagaact ccagcatgag atccccgcgc tggaggatca
     6001 tccagccggc gtcccggaaa acgattccga agcccaacct ttcatagaag gcggcggtgg
     6061 aatcgaaatc tcgtgatggc aggttgggcg tcgcttggtc ggtcatttcg aaccccagag
     6121 tcccgctcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc
     6181 ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat
     6241 atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc
     6301 gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccata
     6361 ggtcacgacg agatcctcgc cgtcgggcat gctcgccttg agcctggcga acagttcggc
     6421 tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat
     6481 ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg
     6541 atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc
     6601 aaggtgagat gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc
     6661 cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga
     6721 tagccgcgct gcctcgtctt gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa
     6781 aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt
     6841 ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg
     6901 caatccatct tgttcaatca tgcgaaacga tcctcatcct gtctcttgat cgatctttgc
     6961 aaaagcctag gcctccaaaa aagcctcctc actacttctg gaatagctca gaggccgggc
     7021 ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact
     7081 gggcggagtt aggggcggaa tgggcggagt taggggcggg actatggttg ctgactaatt
     7141 gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc cacacctggt
     7201 tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt
     7261 tccacaccct aactgacaca cattccacag ctggttcttt ccgcctcagg actcttcctt
     7321 tttcaataaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     7381 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     7441 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     7501 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     7561 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     7621 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     7681 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     7741 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     7801 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     7861 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     7921 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     7981 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     8041 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     8101 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     8161 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     8221 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     8281 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     8341 tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     8401 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     8461 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     8521 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     8581 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     8641 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     8701 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     8761 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttac
//