| 质粒名称: | CP1028-CBEmax |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 119802 |
| 存储实验室: | David Liu |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV with a BR322 origin |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | rApobec1-nSpCas9 (CP1028) -UGI-UGI |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | CP1028-CBEmax was a gift from David Liu (Addgene plasmid # 119802 ; http://n2t.net/addgene:119802 ; RRID:Addgene_119802) |
| 参考文献部分: | Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Huang TP, Zhao KT, Miller SM, Gaudelli NM, Oakes BL, Fellmann C, Savage DF, Liu DR. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y. 10.1038/s41587-019-0134-y PubMed 31110355 |
买家导航
CP1028-CBEmax载体 CP1028CBEmax质粒基本信息
CP1028-CBEmax质粒图谱 CP1028CBEmax载体图谱和CP1028-CBEmax载体序列 CP1028CBEmax质粒序列多克隆位点信息
CP1028-CBEmax质粒 CP1028CBEmax载体简介
CP1028-CBEmax质粒序列 CP1028CBEmax载体序列
LOCUS CP1028-CBEmax 9024 bp ds-DNA circular SYN 19-DEC-2018
DEFINITION C-to-T base editor.
ACCESSION .
VERSION .
KEYWORDS CP1028-CBEmax
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9024)
TITLE Liu lab base editing plasmids
REFERENCE 2 (bases 1 to 9024)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Dec 19, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9024
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 162..541
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 542..745
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 696..716
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 787..806
/label=T7
/note="T7 promoter, forward primer"
promoter 787..805
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 855..875
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 876..1559
/codon_start=1
/product="cytidine deaminase (C to U editing enzyme) from
rat"
/label=APOBEC-1
/note="can use ssDNA as a substrate (Komor et al., 2016)"
/translation="SSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWGG
RHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFLS
RYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSNE
AHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHIL
WATGLK"
CDS 2739..4457
/codon_start=1
/product="N-terminal portion of Streptococcus pyogenes Cas9
(Zetsche et al., 2015)"
/label=Cas9(N)
/translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE"
CDS 5850..6098
/codon_start=1
/product="uracil-DNA glycosylase inhibitor from a Bacillus
subtilis bacteriophage (Mol et al., 1995)"
/label=UGI
/translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
CDS 6129..6377
/codon_start=1
/product="uracil-DNA glycosylase inhibitor from a Bacillus
subtilis bacteriophage (Mol et al., 1995)"
/label=UGI
/translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
CDS 6420..6440
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 6449..6466
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(6489..6506)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 6495..6719
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(6790..6806)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6790..6806)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6803..6825)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 6814..6830
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6838..6868)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6883..6904
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7021..7038)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7192..7780)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7272..7291)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7951..8811)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8574..8593
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8812..8916)
/gene="bla"
/label=AmpR promoter
primer_bind complement(8995..9014)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta
61 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg
121 ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag
181 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
241 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
301 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
361 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
421 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
481 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
541 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
601 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
661 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
721 gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct agagatccgc
781 ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca gccgacggaa
841 gcgagttcga gtcaccaaag aagaagcgga aagtctcctc agagactggg cctgtcgccg
901 tcgatccaac cctgcgccgc cggattgaac ctcacgagtt tgaagtgttc tttgaccccc
961 gggagctgag aaaggagaca tgcctgctgt acgagatcaa ctggggaggc aggcactcca
1021 tctggaggca cacctctcag aacacaaata agcacgtgga ggtgaacttc atcgagaagt
1081 ttaccacaga gcggtacttc tgccccaata ccagatgtag catcacatgg tttctgagct
1141 ggtccccttg cggagagtgt agcagggcca tcaccgagtt cctgtccaga tatccacacg
1201 tgacactgtt tatctacatc gccaggctgt atcaccacgc agacccaagg aataggcagg
1261 gcctgcgcga tctgatcagc tccggcgtga ccatccagat catgacagag caggagtccg
1321 gctactgctg gcggaacttc gtgaattatt ctcctagcaa cgaggcccac tggcctaggt
1381 acccacacct gtgggtgcgc ctgtacgtgc tggagctgta ttgcatcatc ctgggcctgc
1441 ccccttgtct gaatatcctg cggagaaagc agccccagct gaccttcttt acaatcgccc
1501 tgcagtcttg tcactatcag aggctgccac cccacatcct gtgggccaca ggcctgaagt
1561 ctggaggatc tagcggagga tcctctggca gcgagacacc aggaacaagc gagtcagcaa
1621 caccagagag cagtggcggc agcagcggcg gcagcgaaat cggcaaggct accgccaagt
1681 acttcttcta cagcaacatc atgaactttt tcaagaccga gattaccctg gccaacggcg
1741 agatccggaa gcggcctctg atcgagacaa acggcgaaac cggggagatc gtgtgggata
1801 agggccggga ttttgccacc gtgcggaaag tgctgagcat gccccaagtg aatatcgtga
1861 aaaagaccga ggtgcagaca ggcggcttca gcaaagagtc tatcctgccc aagaggaaca
1921 gcgataagct gatcgccaga aagaaggact gggaccctaa gaagtacggc ggcttcgaca
1981 gccccaccgt ggcctattct gtgctggtgg tggccaaagt ggaaaagggc aagtccaaga
2041 aactgaagag tgtgaaagag ctgctgggga tcaccatcat ggaaagaagc agcttcgaga
2101 agaatcccat cgactttctg gaagccaagg gctacaaaga agtgaaaaag gacctgatca
2161 tcaagctgcc taagtactcc ctgttcgagc tggaaaacgg ccggaagaga atgctggcct
2221 ctgccggcga actgcagaag ggaaacgaac tggccctgcc ctccaaatat gtgaacttcc
2281 tgtacctggc cagccactat gagaagctga agggctcccc cgaggataat gagcagaaac
2341 agctgtttgt ggaacagcac aagcactacc tggacgagat catcgagcag atcagcgagt
2401 tctccaagag agtgatcctg gccgacgcta atctggacaa agtgctgtcc gcctacaaca
2461 agcaccggga taagcccatc agagagcagg ccgagaatat catccacctg tttaccctga
2521 ccaatctggg agcccctgcc gccttcaagt actttgacac caccatcgac cggaagaggt
2581 acaccagcac caaagaggtg ctggacgcca ccctgatcca ccagagcatc accggcctgt
2641 acgagacacg gatcgacctg tctcagctgg gaggcgacgg cggatcagga ggctctggcg
2701 gttcaggtgg atcaggcggt agcggaggtt caggtggtat ggacaagaag tacagcatcg
2761 gcctggccat cggcaccaac tctgtgggct gggccgtgat caccgacgag tacaaggtgc
2821 ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag aagaacctga
2881 tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg aagagaaccg
2941 ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag atcttcagca
3001 acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc ttcctggtgg
3061 aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac gaggtggcct
3121 accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac agcaccgaca
3181 aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc cggggccact
3241 tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg ttcatccagc
3301 tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc ggcgtggacg
3361 ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat ctgatcgccc
3421 agctgcccgg cgagaagaag aatggcctgt tcggaaacct gattgccctg agcctgggcc
3481 tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg cagctgagca
3541 aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac cagtacgccg
3601 acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac atcctgagag
3661 tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga tacgacgagc
3721 accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct gagaagtaca
3781 aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac ggcggagcca
3841 gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac ggcaccgagg
3901 aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc ttcgacaacg
3961 gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg cggcaggaag
4021 atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg accttccgca
4081 tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg atgaccagaa
4141 agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag ggcgcttccg
4201 cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac gagaaggtgc
4261 tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg accaaagtga
4321 aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag aaaaaggcca
4381 tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg aaagaggact
4441 acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa gatcggttca
4501 acgcctccct gggcacatac cacgatctgc tgaaaattat caaggacaag gacttcctgg
4561 acaatgagga aaacgaggac attctggaag atatcgtgct gaccctgaca ctgtttgagg
4621 acagagagat gatcgaggaa cggctgaaaa cctatgccca cctgttcgac gacaaagtga
4681 tgaagcagct gaagcggcgg agatacaccg gctggggcag gctgagccgg aagctgatca
4741 acggcatccg ggacaagcag tccggcaaga caatcctgga tttcctgaag tccgacggct
4801 tcgccaacag aaacttcatg cagctgatcc acgacgacag cctgaccttt aaagaggaca
4861 tccagaaagc ccaggtgtcc ggccagggcg atagcctgca cgagcacatt gccaatctgg
4921 ccggcagccc cgccattaag aagggcatcc tgcagacagt gaaggtggtg gacgagctcg
4981 tgaaagtgat gggccggcac aagcccgaga acatcgtgat cgaaatggcc agagagaacc
5041 agaccaccca gaagggacag aagaacagcc gcgagagaat gaagcggatc gaagagggca
5101 tcaaagagct gggcagccag atcctgaaag aacaccccgt ggaaaacacc cagctgcaga
5161 acgagaagct gtacctgtac tacctgcaga atgggcggga tatgtacgtg gaccaggaac
5221 tggacatcaa ccggctgtcc gactacgatg tggaccatat cgtgcctcag agctttctga
5281 aggacgactc catcgacaac aaggtgctga ccagaagcga caagaaccgg ggcaagagcg
5341 acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg cagctgctga
5401 acgccaagct gattacccag agaaagttcg acaatctgac caaggccgag agaggcggcc
5461 tgagcgaact ggataaggcc ggcttcatca agagacagct ggtggaaacc cggcagatca
5521 caaagcacgt ggcacagatc ctggactccc ggatgaacac taagtacgac gagaatgaca
5581 agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc gatttccgga
5641 aggatttcca gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc cacgacgcct
5701 acctgaacgc cgtcgtggga accgccctga tcaaaaagta ccctaagctg gaaagcgagt
5761 tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat gatcgccaag agcgagcaga
5821 gcggcgggag cggcgggagc ggggggagca ctaatctgag cgacatcatt gagaaggaga
5881 ctgggaaaca gctggtcatt caggagtcca tcctgatgct gcctgaggag gtggaggaag
5941 tgatcggcaa caagccagag tctgacatcc tggtgcacac cgcctacgac gagtccacag
6001 atgaaaatgt gatgctgctg acctctgacg cccccgagta taagccttgg gccctggtca
6061 tccaggattc taacggcgag aataagatca agatgctgag cggaggatcc ggaggatctg
6121 gaggcagcac caacctgtct gacatcatcg agaaggagac aggcaagcag ctggtcatcc
6181 aggagagcat cctgatgctg cccgaagaag tcgaagaagt gatcggaaac aagcctgaga
6241 gcgatatcct ggtccatacc gcctacgacg agagtaccga cgaaaatgtg atgctgctga
6301 catccgacgc cccagagtat aagccctggg ctctggtcat ccaggattcc aacggagaga
6361 acaaaatcaa aatgctgtct ggcggctcaa aaagaaccgc cgacggcagc gaattcgagc
6421 ccaagaagaa gaggaaagtc taaccggtca tcatcaccat caccattgag tttaaacccg
6481 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
6541 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
6601 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
6661 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
6721 ttctgaggcg gaaagaacca gctggggctc gataccgtcg acctctagct agagcttggc
6781 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
6841 catacgagcc ggaagcataa agtgtaaagc ctagggtgcc taatgagtga gctaactcac
6901 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
6961 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
7021 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
7081 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
7141 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
7201 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
7261 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
7321 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
7381 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
7441 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
7501 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
7561 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
7621 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
7681 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
7741 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
7801 tacggggtct gacactcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
7861 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
7921 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
7981 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
8041 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
8101 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
8161 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
8221 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
8281 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
8341 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
8401 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
8461 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
8521 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
8581 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
8641 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
8701 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
8761 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
8821 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
8881 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
8941 tgacgtcgac ggatcgggag atcgatctcc cgatccccta gggtcgactc tcagtacaat
9001 ctgctctgat gccgcatagt taag
//
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