| 质粒名称: | pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 103871 |
| 存储实验室: | Feng Zhang |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 5428 bp |
| 完整质粒大小: | 9525 bp |
| 载体修饰: | 人细胞色素c氧化酶亚基VIII（human cytochrome C oxidase subunit VIII） |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | PspCas13b |
| 物种来源: | -- |
| 插入大小: | 2949 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | 人细胞色素c氧化酶亚基VIII（N端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | AgeI(未破坏) |
| 3' 克隆位点: | NotI (未破坏) |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090 was a gift from Feng Zhang (Addgene plasmid # 103871 ; http://n2t.net/addgene:103871 ; RRID:Addgene_103871) |
| 参考文献部分: | RNA editing with CRISPR-Cas13. Cox DBT, Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung J, Zhang F. Science. 2017 Oct 25. pii: eaaq0180. doi: 10.1126/science.aaq0180. 10.1126/science.aaq0180 PubMed 29070703 |
pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090
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pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090载体质粒基本信息
pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090质粒图谱载体图谱和pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090载体序列质粒序列多克隆位点信息
pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090质粒载体简介
pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090质粒序列载体序列
LOCUS pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090 9528 bp ds-DNA circular SYN 29-AUG-2018
DEFINITION dPspCas13b-ADAR2DD(E488Q/T375G) fusion that can be used to
selectively edit adenosine to inosine in RNA when used in conjuction
with a guide RNA. Cas13b is truncated to allow for expression by
AAV.
ACCESSION .
VERSION .
KEYWORDS pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9528)
AUTHORS Cox DBT, Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung
J, Zhang F
TITLE RNA editing with CRISPR-Cas13.
JOURNAL Science. 2017 Oct 25. pii: eaaq0180. doi: 10.1126/science.aaq0180.
PUBMED 29070703
REFERENCE 2 (bases 1 to 9528)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 29, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9528
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 27..406
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 407..610
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 561..581
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 655..674
/label=T7
/note="T7 promoter, forward primer"
promoter 655..673
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 3676..3702
/codon_start=1
/product="nuclear export signal from the HIV Rev protein
(Fischer et al., 1995)"
/label=NES
/translation="LPPLERLTL"
primer_bind complement(4911..4928)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 4917..5141
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 5187..5615
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5274..5293)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5484..5505
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(5624..5644)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 5629..5958
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 5809..5944
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 5871..5890
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 6025..6819
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(6079..6098)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 6689..6708
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 6993..7114
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7030..7049)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7084..7103
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(7163..7179)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7163..7179)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7176..7198)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7187..7203
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7211..7241)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7256..7277
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7394..7411)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7565..8153)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7645..7664)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8324..9184)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8947..8966
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9185..9289)
/gene="bla"
/label=AmpR promoter
primer_bind complement(9364..9383)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
601 aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac
661 gactcactat agggagaccc aagctggcta gcgtttaaac ttaagcttgc caccatgaac
721 atccccgctc tggtggaaaa ccagaagaag tactttggca cctacagcgt gatggccatg
781 ctgaacgctc agaccgtgct ggaccacatc cagaaggtgg ccgatattga gggcgagcag
841 aacgagaaca acgagaatct gtggtttcac cccgtgatga gccacctgta caacgccaag
901 aacggctacg acaagcagcc cgagaaaacc atgttcatca tcgagcggct gcagagctac
961 ttcccattcc tgaagatcat ggccgagaac cagagagagt acagcaacgg caagtacaag
1021 cagaaccgcg tggaagtgaa cagcaacgac atcttcgagg tgctgaagcg cgccttcggc
1081 gtgctgaaga tgtacaggga cctgaccaac gcatacaaga cctacgagga aaagctgaac
1141 gacggctgcg agttcctgac cagcacagag caacctctga gcggcatgat caacaactac
1201 tacacagtgg ccctgcggaa catgaacgag agatacggct acaagacaga ggacctggcc
1261 ttcatccagg acaagcggtt caagttcgtg aaggacgcct acggcaagaa aaagtcccaa
1321 gtgaataccg gattcttcct gagcctgcag gactacaacg gcgacacaca gaagaagctg
1381 cacctgagcg gagtgggaat cgccctgctg atctgcctgt tcctggacaa gcagtacatc
1441 aacatctttc tgagcaggct gcccatcttc tccagctaca atgcccagag cgaggaacgg
1501 cggatcatca tcagatcctt cggcatcaac agcatcaagc tgcccaagga ccggatccac
1561 agcgagaagt ccaacaagag cgtggccatg gatatgctca acgaagtgaa gcggtgcccc
1621 gacgagctgt tcacaacact gtctgccgag aagcagtccc ggttcagaat catcagcgac
1681 gaccacaatg aagtgctgat gaagcggagc agcgacagat tcgtgcctct gctgctgcag
1741 tatatcgatt acggcaagct gttcgaccac atcaggttcc acgtgaacat gggcaagctg
1801 agatacctgc tgaaggccga caagacctgc atcgacggcc agaccagagt cagagtgatc
1861 gagcagcccc tgaacggctt cggcagactg gaagaggccg agacaatgcg gaagcaagag
1921 aacggcacct tcggcaacag cggcatccgg atcagagact tcgagaacat gaagcgggac
1981 gacgccaatc ctgccaacta tccctacatc gtggacacct acacacacta catcctggaa
2041 aacaacaagg tcgagatgtt tatcaacgac aaagaggaca gcgccccact gctgcccgtg
2101 atcgaggatg atagatacgt ggtcaagaca atccccagct gccggatgag caccctggaa
2161 attccagcca tggccttcca catgtttctg ttcggcagca agaaaaccga gaagctgatc
2221 gtggacgtgc acaaccggta caagagactg ttccaggcca tgcagaaaga agaagtgacc
2281 gccgagaata tcgccagctt cggaatcgcc gagagcgacc tgcctcagaa gatcctggat
2341 ctgatcagcg gcaatgccca cggcaaggat gtggacgcct tcatcagact gaccgtggac
2401 gacatgctga ccgacaccga gcggagaatc aagagattca aggacgaccg gaagtccatt
2461 cggagcgccg acaacaagat gggaaagaga ggcttcaagc agatctccac aggcaagctg
2521 gccgacttcc tggccaagga catcgtgctg tttcagccca gcgtgaacga tggcgagaac
2581 aagatcaccg gcctgaacta ccggatcatg cagagcgcca ttgccgtgta cgatagcggc
2641 gacgattacg aggccaagca gcagttcaag ctgatgttcg agaaggcccg gctgatcggc
2701 aagggcacaa cagagcctca tccatttctg tacaaggtgt tcgcccgcag catccccgcc
2761 aatgccgtcg agttctacga gcgctacctg atcgagcgga agttctacct gaccggcctg
2821 tccaacgaga tcaagaaagg caacagagtg gatgtgccct tcatccggcg ggaccagaac
2881 aagtggaaaa cacccgccat gaagaccctg ggcagaatct acagcgagga tctgcccgtg
2941 gaactgccca gacagatgtt cgacaatgag atcaagtccc acctgaagtc cctgccacag
3001 atggaaggca tcgacttcaa caatgccaac gtgacctatc tgatcgccga gtacatgaag
3061 agagtgctgg acgacgactt ccagaccttc taccagtgga accgcaacta ccggtacatg
3121 gacatgctta agggcgagta cgacagaaag ggctccctgc agcactgctt caccagcgtg
3181 gaagagagag aaggcctctg gaaagagcgg gcctccagaa cagagcggta cagaaagcag
3241 gccagcaaca agatccgcag caaccggcag atgagaaacg ccagcagcga agagatcgag
3301 acaatcctgg ataagcggct gagcaacagc cggaacgagt accagaaaag cgagaaagtg
3361 atccggcgct acagagtgca ggatgccctg ctgtttctgc tggccaaaaa gaccctgacc
3421 gaactggccg atttcgacgg cgagaggttc aaactgaaag aaatcatgcc cgacgccgag
3481 aagggaatcc tgagcgagat catgcccatg agcttcacct tcgagaaagg cggcaagaag
3541 tacaccatca ccagcgaggg catgaagctg aagaactacg gcgacttctt tgtgctggct
3601 agcgacaaga ggatcggcaa cctgctggaa ctcgtgggca gcgacatcgt gtccaaagag
3661 gatggatccc ttcaactgcc tccacttgaa agactgacac tgggatccca gctgcattta
3721 ccgcaggttt tagctgacgc tgtctcacgc ctggtcctgg gtaagtttgg tgacctgacc
3781 gacaacttct cctcccctca cgctcgcaga aaagtgctgg ctggagtcgt catgacaaca
3841 ggcacagatg ttaaagatgc caaggtgata agtgtttcta caggaggcaa atgtattaat
3901 ggtgaataca tgagtgatcg tggccttgca ttaaatgact gccatgcaga aataatatct
3961 cggagatcct tgctcagatt tctttataca caacttgagc tttacttaaa taacaaagat
4021 gatcaaaaaa gatccatctt tcagaaatca gagcgagggg ggtttaggct gaaggagaat
4081 gtccagtttc atctgtacat cagcacctct ccctgtggag atgccagaat cttctcacca
4141 catgagccaa tcctggaaga accagcagat agacacccaa atcgtaaagc aagaggacag
4201 ctacggacca aaatagagtc tggtcagggg acgattccag tgcgctccaa tgcgagcatc
4261 caaacgtggg acggggtgct gcaaggggag cggctgctca ccatgtcctg cagtgacaag
4321 attgcacgct ggaacgtggt gggcatccag ggatcactgc tcagcatttt cgtggagccc
4381 atttacttct cgagcatcat cctgggcagc ctttaccacg gggaccacct ttccagggcc
4441 atgtaccagc ggatctccaa catagaggac ctgccacctc tctacaccct caacaagcct
4501 ttgctcagtg gcatcagcaa tgcagaagca cggcagccag ggaaggcccc caacttcagt
4561 gtcaactgga cggtaggcga ctccgctatt gaggtcatca acgccacgac tgggaaggat
4621 gagctgggcc gcgcgtcccg cctgtgtaag cacgcgttgt actgtcgctg gatgcgtgtg
4681 cacggcaagg ttccctccca cttactacgc tccaagatta ccaagcccaa cgtgtaccat
4741 gagtccaagc tggcggcaaa ggagtaccag gccgccaagg cgcgtctgtt cacagccttc
4801 atcaaggcgg ggctgggggc ctgggtggag aagcccaccg agcaggacca gttctcactc
4861 acgtaagcgg ccgctcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt
4921 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
4981 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
5041 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
5101 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
5161 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
5221 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
5281 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
5341 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
5401 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
5461 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
5521 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
5581 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
5641 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
5701 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
5761 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
5821 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
5881 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
5941 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa
6001 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
6061 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
6121 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
6181 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
6241 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
6301 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
6361 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
6421 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
6481 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
6541 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
6601 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
6661 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
6721 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
6781 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
6841 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
6901 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
6961 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
7021 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
7081 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
7141 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
7201 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
7261 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
7321 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
7381 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
7441 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
7501 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
7561 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
7621 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
7681 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
7741 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
7801 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
7861 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
7921 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
7981 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
8041 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
8101 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
8161 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
8221 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
8281 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
8341 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
8401 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
8461 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
8521 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
8581 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
8641 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
8701 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
8761 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
8821 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
8881 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
8941 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
9001 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
9061 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
9121 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
9181 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
9241 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
9301 gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct
9361 cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt
9421 ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga
9481 caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcg
//
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pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090质粒载体应用举例
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