| 质粒名称: | pSL1341 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002431 |
| 产品用途: | Mammalian PhCMV-driven constitutive expression vector for a LIRP variant where amino acid residue G55 was deleted from MCP(LOV2 53/54) (PhCMV -MCP(SA-LOV2 53/54)-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | PhCMV |
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | MCP(SA-LOV2 53/54) |
| 物种来源: | synthetic |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Rationally designed light-inducible RNA-releasing protein for translational regulation and optogenetic control of gene therapies
NA
NA
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pSL1341
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pSL1341载体质粒基本信息
pSL1341质粒图谱载体图谱和pSL1341载体序列质粒序列多克隆位点信息
pSL1341质粒载体简介
pSL1341质粒序列载体序列
LOCUS pSL1341 6210 bp DNA circular SYN 05-MAR-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6210)
AUTHORS Trial User
TITLE Direct Submission
JOURNAL Exported Aug 28, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6210
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 232..819
/label=CMV
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 863..882
/label=T7
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 880..923
/label=oSCL2210
primer_bind 886..918
/label=Oscl32-1
primer_bind 886..918
/label=oSCL1118
primer_bind 901..918
/label=Oscl42-1
regulatory 901..910
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
regulatory 902..910
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 910..1068
/codon_start=1
/product="bacteriophage MS2 coat protein"
/label=MS2-N55K
/note="binds with high affinity to a specific stem-loop
structure in the viral RNA (Lim et al., 1994)"
/translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
CSVRQSSA"
primer_bind complement(1004..1085)
/label=oSCL2215
misc_feature 1023^1024
/label=Feature 16
primer_bind 1024..1043
/label=oSCL2216
primer_bind 1043..1086
/label=oSCL2328
primer_bind 1043..1086
/label=oSCL2329
primer_bind 1043..1086
/label=oSCL2330
primer_bind complement(1043..1062)
/label=oSCL2327
primer_bind complement(1051..1085)
/label=oSCL2217
misc_feature 1069..1479
/label=LOV2
primer_bind 1069..1086
/label=oSCL1757
primer_bind complement(1069..1085)
/label=oSCL2219
primer_bind complement(1458..1479)
/label=oSCL1758
primer_bind 1460..1506
/label=oSCL2218
primer_bind 1460..1479
/label=oSCL2216
primer_bind 1460..1479
/label=oSCL2220
CDS 1480..1707
/codon_start=1
/product="bacteriophage MS2 coat protein"
/label=MS2-N55K
/note="binds with high affinity to a specific stem-loop
structure in the viral RNA (Lim et al., 1994)"
/translation="QKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATN
SDCELIVKAMQGLLKDGNPIPSAIAANSGIY"
misc_feature 1480
/label=Feature 17
primer_bind complement(1581..1602)
/label=oSCL2219
primer_bind 1602..1622
/label=oSCL2220
misc_feature 1602^1603
/label=Feature 18
primer_bind complement(1687..1730)
/label=oSCL2211
primer_bind complement(1687..1716)
/label=Oscl32-2
primer_bind complement(1687..1707)
/label=oSCL093
primer_bind complement(1687..1707)
/label=oSCL396
terminator 1807..2034
/label=bGH PA
polyA_signal 1810..2034
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 2080..2508
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2522..2851
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2702..2837
/label=SV40 ori
/note="SV40 origin of replication"
misc_feature 2702..2779
/label=SV40 ORI
CDS 2918..3712
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
CDS 2921..3712
/label=Kan/neoR
polyA_signal 3886..4007
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4052..4072)
/label=M13-rev
primer_bind complement(4056..4072)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
misc_binding complement(4078..4100)
/label=LacO
protein_bind 4080..4096
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4104..4134)
/label=lac promoter
/note="promoter for the E. coli lac operon"
promoter complement(4105..4134)
/label=lac
protein_bind 4149..4170
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(4458..5043)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5214..6074)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
CDS complement(5217..5876)
/label=AmpR
promoter complement(6075..6179)
/gene="bla"
/label=AmpR promoter
promoter complement(6116..6144)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gccaccatgg cttcaaactt tactcagttc gtgctcgtgg acaatggtgg gacaggggat
961 gtgacagtgg ctccttctaa tttcgctaat ggggtggcag agtggatcag ctccaactca
1021 cggagccagg cctacaaggt gacatgcagc gtcaggcagt ctagtgcctt ggaacgtatc
1081 gaaaagaatt ttgtcatcac ggacccgcgt ctgcccgaca atccgattat cttcgcgtca
1141 gactctttct tacaactgac tgagtatagt agagaggaga tattggggcg taactgtaga
1201 tttcttcagg ggccagaaac tgatcgggct accgttcgca agatacgtga cgcaatagac
1261 aaccagaccg aggtgacggt gcagctgatt aactacacaa agtctgggaa gaagttctgg
1321 aacctgtttc atttacaacc tatgagggac caaaaaggtg acgttcaata tttcatcggg
1381 gttcagttag atgggactga gcacgtgaga gatgcagcag aaagagaggg tgtaatgctt
1441 attaaaaaaa cagccgagaa tatcgacgaa gccgctggtc agaagagaaa gtataccatc
1501 aaggtggagg tccccaaagt ggctacccag acagtgggcg gagtcgaact gcctgtcgcc
1561 gcttggaggt cctacctgaa catggagctc actatcccaa ttttcgctac caattctgac
1621 tgtgaactca tcgtgaaggc aatgcagggg ctcctcaaag acggtaatcc tatcccttcc
1681 gccatcgccg ctaactcagg tatctactaa ggatccacta gtccagtgtg gtggaattct
1741 gcagatatcc agcacagtgg cggccgctcg agtctagagg gcccgtttaa acccgctgat
1801 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
1861 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
1921 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
1981 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
2041 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
2101 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
2161 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
2221 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
2281 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
2341 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
2401 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
2461 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa
2521 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
2581 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
2641 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
2701 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
2761 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
2821 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
2881 cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
2941 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
3001 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
3061 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
3121 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
3181 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
3241 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
3301 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
3361 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
3421 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
3481 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
3541 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
3601 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
3661 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
3721 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
3781 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
3841 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
3901 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
3961 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
4021 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
4081 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
4141 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
4201 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
4261 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
4321 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
4381 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
4441 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
4501 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
4561 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
4621 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
4681 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
4741 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
4801 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
4861 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
4921 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
4981 accgctggta gcggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
5041 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
5101 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
5161 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
5221 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
5281 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
5341 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
5401 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
5461 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
5521 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
5581 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
5641 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
5701 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
5761 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
5821 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
5881 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
5941 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
6001 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
6061 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
6121 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
6181 acatttcccc gaaaagtgcc acctgacgtc
//
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