pSBbi-mNeptusFT2-hGeminin(1-110)-Puromycin

LOCUS      pSBbi-mNeptusFT2-hGeminin(1-110)-Puromycin              7462 bp DNA     circular SYN 23-DEC-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 29, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        109..213
                     /gene="bla"
                     /label=AmpR promoter
     CDS             214..1074
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1245..1833
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      2318..2429
                     /label=rabbit globin terminator
     CDS             complement(2616..3215)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDCHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     promoter        3885..5066
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     CDS             5108..6529
                     /codon_start=1
                     /label=mNeptusFT2
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTGRIKV
                     VEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRCNMALK
                     LVGGGHLICNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHKLNEFMVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(5445..5462)
                     /label=mNeptusFT2-Rseq2
     CDS             5813..6529
                     /codon_start=1
                     /product="enhanced CFP with monomerizing A206K mutation"
                     /label=mECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             6539..6871
                     /codon_start=1
                     /label=hGeminin(1-110)
                     /translation="MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELS
                     AGLSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWKEVAE
                     KRRKAL"
     polyA_signal    6915..7139
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 aattttaaca aaatattaac gcttacaatt tcctgatgcg gtattttctc cttacgcatc
       61 tgtgcggtat ttcacaccgc atcaggtggc acttttcggg gaaatgtgcg cggaacccct
      121 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
      181 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
      241 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
      301 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
      361 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
      421 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
      481 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
      541 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
      601 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
      661 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
      721 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
      781 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
      841 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
      901 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
      961 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     1021 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     1081 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     1141 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     1201 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     1261 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     1321 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     1381 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     1441 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     1501 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     1561 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     1621 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     1681 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     1741 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     1801 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     1861 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     1921 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgagcgacc
     1981 gagtgcagcg agtcagtgag cgaggaagcg gatccctata cagttgaagt cggaagttta
     2041 catacacctt agccaaatac atttaaactc actttttcac aattcctgac atttaatcct
     2101 agtaaaaatt ccctgtctta ggtcagttag gatcaccact ttattttaag aatgtgaaat
     2161 atcagaataa tagtagagag aatgattcat ttcagctttt atttctttca tcacattccc
     2221 agtgggtcag aagtttacat acactcgact cctctgcaga atgcggcgat gtttcggtaa
     2281 ggggtcccga gtagctagtt catggcagcc agcatatggc atatgttgcc aaactctaaa
     2341 ccaaatactc attctgatgt tttaaatgat ttgccctccc atatgtcctt ccgagtgaga
     2401 gacacaaaaa attccaacac actattgcaa tgaaaataaa tttcctttat tagccagaag
     2461 tcagatgctc aaggggcttc atgatgtccc cataattttt ggcagaggga aaaagatctc
     2521 agtggtattt gtgagccagg gcattggcca caccagccac caccttctga taggcagcct
     2581 gcacctgagg agtgaattca cgacaggcct tcgaattacg caccgggctt gcgggtcatg
     2641 caccaggtgc gcggtccttc gggcacctcg acgtcggcgg tgacggtgaa gccgagccgc
     2701 tcgtagaagg ggaggttgcg gggcgcggag gtctccagga aggcgggcac cccggcgcgc
     2761 tcggccgcct ccactccggg gagcacgacg gcgctgccca gacccttgcc ctggtggtcg
     2821 ggcgagacgc cgacggtggc caggaaccac gcgggctcct tgggccggtg cggcgccagg
     2881 aggccttcca tctgttgctg cgcggccagc cgggaaccgc tcaactcggc catgcgcggg
     2941 ccgatctcgg cgaacaccgc ccccgcttcg acgctctccg gcgtggtcca gaccgccacc
     3001 gcggcgccgt cgtccgcgac ccacaccttg ccgatgtcga gcccgacgcg cgtgaggaag
     3061 agttcttgca gctcggtgac ccgctcgatg tggcagtccg ggtcgacggt gtggcgcgtg
     3121 gcggggtagt cggcgaacgc ggcggcgagg gtgcgtacgg cccgggggac gtcgtcgcgg
     3181 gtggcgaggc gcaccgtggg cttgtactcg gtcatgcggc cgctctcgcc cttgctcaac
     3241 atggtggcgg cgcgccctca ggtgcagagg ttttctacag ggaaagagag aaaggctaca
     3301 gttagtttgc tgtgatgatg ctaagtgccg aggtcagatg tctatggatt gtacacggaa
     3361 tcccaacgca ggaaccaaaa aacactcacc caaaaaaaat ggcgctaagg ccgagagaaa
     3421 ggcgaagttc cgtctacggc tatatacctt gaaacagggg cggcagaagt gacgcaacgc
     3481 ggttgcgcgg acggcttcct gtgttttcat tggtcgaagc gttctgcgcg tgcgcagaga
     3541 ttccccaggg aaggcgggcc ctcttccgaa tgtccaatcc ggacattctt ccggttggac
     3601 ctccgcggtc agcgctgtgc ccggcgagag atcacgtggg gcgcggaggc ggtgctgctg
     3661 gggcacggcc gtccagcctc ggcggccata tttttgaggg gctgttcatc tcgttcacac
     3721 gctctgtccg ccatgtttgt gagtggaagc gccattaccc cttcaagcga ctgaaggctg
     3781 cagggcctct ggtggcccgc atggggagac cagacccgcc aggcccgcct ttccgcactc
     3841 agtccgggct tactttattt tgtgagacag ggtctcgcct agaggctccg gtgcccgtca
     3901 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     3961 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     4021 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     4081 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
     4141 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cgcccctggc
     4201 tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc
     4261 cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggctt gggcgctggg
     4321 gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc
     4381 tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg
     4441 taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg
     4501 gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga
     4561 gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc
     4621 cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg
     4681 aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg
     4741 gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg
     4801 cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct
     4861 tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccata
     4921 ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat
     4981 ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt
     5041 tttttcttcc atttaaggtg tcgtgaaaac taccccaagc tggcctctga ggccaggtac
     5101 cgccaccatg agcgagctga ttaaggagaa catgcacatg aagctgtaca tggagggcac
     5161 cgtgaacaac caccacttca agtgcacatc cgagggcgaa ggcaagccct acgagggcac
     5221 ccagaccggc agaatcaagg tggtcgaggg cggccctctc cccttcgcct tcgacatcct
     5281 ggctacctgc ttcatgtacg gcagcaagac cttcatcaac cacacccagg gcatccccga
     5341 cttctttaag cagtccttcc ctgagggctt cacatgggag agagtcacca catacgaaga
     5401 cgggggcgtg ctgaccgcta cccaggacac cagcctccag gacggctgcc tcatctacaa
     5461 cgtcaagatc agaggggtga acttcccatc caacggccct gtgatgcaga agaaaacact
     5521 cggctgggag gcctccaccg agacgctgta ccccgccgac ggcggcctgg aaggcagatg
     5581 taacatggcc ctgaagctcg tgggcggggg ccacctgatc tgcaacttga agaccacata
     5641 cagatccaag aaacccgcta agaacctcaa gatgcccggc gtctacttcg tggaccgcag
     5701 actggaaaga atcaaggagg ccgacaaaga gacctatgtc gagcagcacg aggtggctgt
     5761 ggccagatac tgcgacctcc ctagcaaact cggccataag ctcaatgaat tcatggtgag
     5821 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     5881 aaacggccac aagttcagcg tgcgcggcga gggcgagggc gatgccacca acggcaagct
     5941 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     6001 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcgccacga
     6061 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatca gcttcaagga
     6121 cgacggcacc tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     6181 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     6241 gtacaacttc aacagccaca acgtctatat caccgccgac aagcagaaga acggcatcaa
     6301 ggccaacttc aagatccgcc acaacgtgga ggacggcagc gtgcagctcg ccgaccacta
     6361 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     6421 cacccagtcc gtgctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     6481 gttcgtgacc gccgccggga tcactcacgg catggacgag ctgtacaagc gaccggttat
     6541 gaatcccagt atgaagcaga aacaagaaga aatcaaagag aatataaaga atagttctgt
     6601 cccaagaaga actctgaaga tgattcagcc ttctgcatct ggatctcttg ttggaagaga
     6661 aaatgagctg tccgcaggct tgtccaaaag gaaacatcgg aatgaccact taacatctac
     6721 aacttccagc cctggggtta ttgtcccaga atctagtgaa aataaaaatc ttggaggagt
     6781 cacccaggag tcatttgatc ttatgattaa agaaaatcca tcctctcagt attggaagga
     6841 agtggcagaa aaacggagaa aggcgctgta aaagcttggg gatcaattct ctagagctcg
     6901 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     6961 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     7021 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     7081 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     7141 ttctgaggcg gaaagaacca gctgggccta ctagatagcg gaccccttac cgaaacatcg
     7201 ccgcattctg cagaggagtc gaacttagtg tatgtaaact tctgacccac tggaattgtg
     7261 atacagtgaa ttataagtga aataatctgt ctgtaaacaa ttgttggaaa aatgacttgt
     7321 gtcatgcaca aagtagatgt cctaactgac ttgccaaaac tattgtttgt taacaagaaa
     7381 tttgtggagt agttgaaaaa cgagttttaa tgactccaac ttaagtgtat gtaaacttcc
     7441 gacttcaact gtatagggat cc
//