| 质粒名称: | pYK664 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000836 |
| 产品用途: | Mammalian PhCMV-driven constitutive GNCR-ZFPVEGFA-3xFLAG-NLS expression vector (PhCMV-GNCR-ZFPVEGFA-3xFLAG-NLS-pA). |
| 存储实验室: | Anping ZENG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: |
GNCR-ZFPVEGFA-3xFLAG-NLS
|
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Programmable solid-state condensates for spatiotemporal control of mammalian gene expression
Wang Y., Jiang J., Xiong Q., Li S., Shao J., Xie M., Zeng A.P.
Nat. Chem. Biol. 2025 1–10 (2025)
|
pYK664
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pYK664载体质粒基本信息
pYK664质粒图谱载体图谱和pYK664载体序列质粒序列多克隆位点信息
pYK664质粒载体简介
pYK664质粒序列载体序列
LOCUS pYK664 6591 bp DNA circular SYN 11-FEB-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6591)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 28, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6591
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 232..819
/label=CMV
primer_bind 863..882
/label=T7
primer_bind 880..946
/label=oYF0019
primer_bind 881..918
/label=oYK421_GNCR_F
primer_bind 881..902
/label=oYK31-mCherry-NS3a-F
primer_bind 910..952
/label=oYF005
primer_bind 916..937
/label=oYK481_GNCR_F
primer_bind 916..937
/label=oYK483_LCK1_F
primer_bind 916..936
/label=oYK490_P2A_GNCR1_F
CDS 919..1617
/codon_start=1
/label=GNCR1-N
/translation="DIEKLCKKAEEEAKEAQEKADELRQRHPDSQAAEDAEDLANLAVA
AVLTACLLAQEHPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQAA
RAVILAIMLAAENPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQA
AEAVERAIWLAAENPNADIAKKCIKAASEAAEEASKAAEEAQRHPDSQKARDEIKEASQ
KAEEVKERCKS"
primer_bind 919..952
/label=oYF0011
primer_bind 919..943
/label=oLZ146 GNCR1-N F
primer_bind 919..943
/label=oSCL762
primer_bind 1585..1597
/label=oYK03-02-F
primer_bind 1585..1596
/label=oYK31-mCherry-NS3a-F
primer_bind complement(1596..1653)
/label=oYF0020
primer_bind complement(1596..1647)
/label=oYF006
primer_bind complement(1596..1618)
/label=oYF0012
primer_bind complement(1605..1624)
/label=oYK465_GNCR1_R
primer_bind 1615..1637
/label=oSCL1129
primer_bind 1615..1637
/label=oSCL1192
primer_bind 1615..1635
/label=oLZ147 NS3a F
primer_bind 1615..1634
/label=oYF0033
primer_bind 1617..1637
/label=oLZ048 NSP3-F
primer_bind 1617..1634
/label=oYK419_GS_F
primer_bind 1618..1647
/label=oSCL388
primer_bind 1618..1633
/label=oYK28-mCherry-NS3a-F
primer_bind complement(1619..1667)
/label=oLZ145 DNCR2-GNCR1 R
primer_bind complement(1622..1637)
/label=oYK520_GS_R
primer_bind complement(1625..1643)
/label=Oscl18-4
primer_bind complement(1628..1648)
/label=oSCL1119
primer_bind complement(1628..1647)
/label=oSCL075
primer_bind complement(1628..1647)
/label=oSCL075a
primer_bind complement(1628..1647)
/label=oSCL075`b
primer_bind complement(1628..1647)
/label=oSCL075`c
primer_bind complement(1631..1647)
/label=oYK472_NLS_R
primer_bind 1632..1652
/label=oLZ146 GNCR1-N F
primer_bind complement(1632..1648)
/label=oYK92_L7Ae_R
primer_bind complement(1634..1662)
/label=oYK489_GNCR1_R
misc_feature 1634..1653
/label=linker
primer_bind complement(1636..1648)
/label=oYK75_NLS_mcp_R
primer_bind complement(1645..1668)
/label=oYK421_GNCR3_R
primer_bind 1649..1690
/label=oYK664_ZFvegfa_F
misc_feature 1654..1668
/label=NSP3
CDS 1669..1950
/codon_start=1
/label=Zinc Finger (VEGFA)
/translation="MGKKKQHICHIQGCGKVYGTTSNLRRHLRWHTGERPFMCTWSYCG
KRFTRSSNLQRHKRTHTGEKKFACPECPKRFMRSDHLSRHIKTHQNKKG"
primer_bind 1669..1696
/label=oYK1233_VEGFA_F
primer_bind complement(1951..1974)
/label=oYK563_ZF_R
primer_bind 1955..1978
/label=oYK563_Flag_F
CDS 1960..2025
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
primer_bind complement(2000..2063)
/label=oYK502_ZF_R
CDS 2032..2052
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
primer_bind 2054..2075
/label=oSCL1129
primer_bind 2054..2075
/label=oSCL1192
primer_bind 2054..2073
/label=oLZ147 NS3a F
primer_bind 2054..2072
/label=oYF0033
misc_feature 2056..2085
/label=linker
primer_bind 2056..2085
/label=oSCL388
misc_feature 2056..2076
/label=linker
primer_bind 2056..2075
/label=oLZ048 NSP3-F
primer_bind 2056..2072
/label=oYK419_GS_F
primer_bind 2056..2071
/label=oYK28-mCherry-NS3a-F
primer_bind complement(2057..2085)
/label=oLZ145 DNCR2-GNCR1 R
primer_bind complement(2060..2075)
/label=oYK520_GS_R
primer_bind complement(2063..2081)
/label=Oscl18-4
primer_bind complement(2066..2085)
/label=oSCL075
primer_bind complement(2066..2085)
/label=oSCL075a
primer_bind complement(2066..2085)
/label=oSCL075`b
primer_bind complement(2066..2085)
/label=oSCL1119
primer_bind complement(2066..2085)
/label=oSCL075`c
primer_bind complement(2069..2111)
/label=oYK472_NLS_R
primer_bind 2070..2085
/label=oLZ146 GNCR1-N F
primer_bind complement(2070..2085)
/label=oYK92_L7Ae_R
primer_bind complement(2072..2085)
/label=oYK489_GNCR1_R
primer_bind complement(2074..2085)
/label=oYK75_NLS_mcp_R
primer_bind complement(2089..2111)
/label=oYK57-MCP-mCherry-R
terminator 2185..2412
/label=bGH PA
misc_feature 2458..2886
/label=f1 origin
misc_feature 2891..3234
/label=SV40 early promoter and origin
misc_feature 3080..3157
/label=SV40 ORI
CDS 3299..4090
/label=Kan/neoR
misc_feature 4264..4394
/label=SV40 early polyadenylation signal
primer_bind complement(4430..4450)
/label=M13-rev
misc_binding complement(4456..4478)
/label=LacO
promoter complement(4483..4512)
/label=lac
misc_feature complement(4777..5450)
/label=pUC origin
CDS complement(5598..6257)
/label=AmpR
promoter complement(6497..6525)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gctggctagg ccaccatgga catcgagaag ctgtgcaaga aggcggaaga ggaggccaag
961 gaggcccagg agaaggccga cgagctgaga caacgacacc ccgattcgca ggcggcggaa
1021 gacgcggagg atctcgcaaa tctggctgtc gccgccgtgc tgaccgcttg tctgctggcc
1081 caggagcatc ccaacgccga tatcgctaag ctgtgcatca aggccgcgag cgaagctgcg
1141 gaggccgcgt caaaagccgc cgagctggcc caacggcacc cagacagtca agcagccaga
1201 gatgctatca aactcgcctc tcaagccgcc cgcgccgtga tcctggccat catgctcgcc
1261 gcagagaatc ccaacgctga tattgcaaag ctatgtatta aggcggccag cgaagccgca
1321 gaggctgcct cgaaagctgc ggaacttgca cagcggcacc ccgactccca ggccgcccga
1381 gatgcaatta aactggccag tcaggccgct gaggcggtgg agagagccat ctggctggcc
1441 gccgagaacc ccaatgccga tatcgccaaa aaatgcatca aagcggcctc cgaagcagcc
1501 gaggaggcct ctaaagctgc cgaagaggct caaagacacc cagacagcca gaaggcacgc
1561 gacgagatca aggaggcctc tcagaaggcc gaggaggtga aggagagatg caagagcgcc
1621 agcggaagtg gcggaggagg cgacgtcgct agcatgctca agatggagat gggcaaaaag
1681 aagcagcaca tctgccacat ccagggatgc ggcaaggtgt acggcaccac cagcaacctg
1741 aggaggcacc ttaggtggca caccggggag aggcccttca tgtgcacctg gagctactgc
1801 ggaaaaaggt tcaccaggag cagcaacttg cagaggcaca agaggaccca cacaggcgag
1861 aagaagttcg cctgccccga gtgccccaag aggtttatga ggagcgacca tctgagcagg
1921 cacatcaaga cccaccagaa caagaaaggc acctgcagag actacaaaga ccatgacggc
1981 gactataaag atcacgacat cgattacaaa gacgacgacg acaagatggc tccaaagaag
2041 aagagaaagg tgggtgccag cggaagtggc ggaggaggcg acgtctaagg atccactagt
2101 ccagtgtggt ggaattctgc agatatccag cacagtggcg gccgctcgag tctagagggc
2161 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
2221 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
2281 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
2341 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
2401 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
2461 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
2521 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
2581 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
2641 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
2701 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
2761 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
2821 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
2881 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
2941 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
3001 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
3061 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
3121 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
3181 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
3241 agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat
3301 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
3361 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
3421 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
3481 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
3541 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
3601 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
3661 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
3721 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
3781 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
3841 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
3901 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
3961 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
4021 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
4081 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
4141 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
4201 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
4261 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
4321 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
4381 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
4441 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
4501 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
4561 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
4621 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
4681 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
4741 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
4801 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
4861 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
4921 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
4981 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
5041 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
5101 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
5161 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
5221 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
5281 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
5341 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
5401 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
5461 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
5521 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
5581 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
5641 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
5701 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
5761 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
5821 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
5881 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
5941 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
6001 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
6061 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
6121 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
6181 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
6241 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
6301 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
6361 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
6421 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
6481 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
6541 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c
//
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