| 出品公司: | Invivogen |
|---|---|
| 载体名称: | pFUSE-hIgG1e2-Fc2 |
| 载体又名: | pFUSE-hIgG1e2-Fc2载体，pFUSE-hIgG1e2-Fc2质粒，pFUSE-hIgG1e2-Fc2载体质粒 |
| 英文名称: | pFUSE-hIgG1e2-Fc2, pFUSE-hIgG1e2-Fc2 Vector, pFUSE-hIgG1e2-Fc2 Plasmid |
| 质粒类型: | 哺乳动物细胞表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 4.2 kb |
| 5’测序引物: | -- |
| 3’测序引物: | -- |
| 载体标签: | -- |
| 载体抗性: | Zeocin |
| 筛选标记: | 无 |
| 克隆菌株: | DH5α,TOP10 等 |
| 宿主细胞: | 常规细胞系（293、CHO等） |
| 备注: |
pFUSE-hIgG1e2-Fc2载体说明书 pFUSE-hIgG1e2-Fc2载体序列 |
| 产品目录号: | 21756894 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pFUSE-hIgG1e2-Fc2载体质粒基本信息
pFUSE-hIgG1e2-Fc2质粒图谱载体图谱和pFUSE-hIgG1e2-Fc2载体序列质粒序列多克隆位点信息
pFUSE-hIgG1e2-Fc2质粒载体简介
pFUSE-hIgG1e2-Fc2质粒序列载体序列
pFUSE-hIgG1e2-Fc2 Snapgene格式序列 pFUSE-hIgG1e2-Fc2 .gb格式序列
LOCUS pFUSE-hIgG1e2-Fc2 4194 bp DNA circular SYN 31-JUL-2025
DEFINITION .
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4194)
AUTHORS DotmaticsRD
TITLE Direct Submission
JOURNAL Exported Jul 31, 2025 from SnapGene 8.1.0
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4194
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 31..242
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
/note="color: #ffffff; direction: RIGHT"
LTR 255..523
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
/note="color: #ffe4c4"
sig_peptide 573..632
/codon_start=1
/product="human interleukin-2 signal sequence"
/label=IL-2 signal sequence
/note="color: #993366
Cleavage site after base 632"
/translation="MYRMQLLSCIALSLALVTNS"
polyA_signal 1359..1480
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
/note="color: #a6acb3"
polyA_signal 1584..1978
/label=beta-globin poly(A)
/note="human beta-globin polyadenylation signal"
/note="color: #a6acb3"
CDS complement(1992..2366)
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/note="color: #ccffcc"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
enhancer 2959..3262
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
/note="color: #ffffff"
rep_origin complement(3342..3930)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
/note="color: #ffff00"
polyA_signal 4038..4086
/note="synthetic polyadenylation signal"
/note="color: #a6acb3"
misc_feature 4097..4188
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
/note="color: #a6acb3"
ORIGIN
1 ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
61 agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa
121 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
181 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
241 agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc
301 gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg
361 cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc
421 cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac
481 tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc
541 ctacctgaga tcaccggcga aggagggcca ccatgtacag gatgcaactc ctgtcttgca
601 ttgcactaag tcttgcactt gtcacgaatt cgatatcggc catggttaga tctgacaaaa
661 ctcacacatg cccaccgtgc ccagcacctg aactcctggg gggaccgtca gtcttcctct
721 tccccccaaa acccaaggac accctctaca tcacccggga acctgaggtc acatgcgtgg
781 tggtggacgt gagccacgaa gaccctgagg tcaagttcaa ctggtacgtg gacggcgtgg
841 aggtgcataa tgccaagaca aagccgcggg aggagcagta caacagcacg taccgtgtgg
901 tcagcgtcct caccgtcctg caccaggact ggctgaatgg caaggagtac aagtgcaagg
961 tctccaacaa agccctccca gcccccatcg agaaaaccat ctccaaagcc aaagggcagc
1021 cccgagaacc acaggtgtac accctgcccc catcccggga ggagatgacc aagaaccagg
1081 tcagcctgac ctgcctggtc aaaggcttct atcccagcga catcgccgtg gagtgggaga
1141 gcaatgggca gccggagaac aactacaaga ccacgcctcc cgtgctggac tccgacggct
1201 ccttcttcct ctacagcaag ctcaccgtgg acaagagcag gtggcagcag gggaacgtct
1261 tctcatgctc cgtgatgcat gaggctctga agttccacta cacgcagaag agcctctccc
1321 tgtctccggg taaatgagtg ctagctggcc agacatgata agatacattg atgagtttgg
1381 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
1441 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
1501 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta
1561 caaatgtggt atggaattaa ttctaaaata cagcatagca aaactttaac ctccaaatca
1621 agcctctact tgaatccttt tctgagggat gaataaggca taggcatcag gggctgttgc
1681 caatgtgcat tagctgtttg cagcctcacc ttctttcatg gagtttaaga tatagtgtat
1741 tttcccaagg tttgaactag ctcttcattt ctttatgttt taaatgcact gacctcccac
1801 attccctttt tagtaaaata ttcagaaata atttaaatac atcattgcaa tgaaaataaa
1861 tgttttttat taggcagaat ccagatgctc aaggcccttc ataatatccc ccagtttagt
1921 agttggactt agggaacaaa ggaaccttta atagaaattg gacagcaaga aagcgagctt
1981 ctagcttatc ctcagtcctg ctcctctgcc acaaagtgca cgcagttgcc ggccgggtcg
2041 cgcagggcga actcccgccc ccacggctgc tcgccgatct cggtcatggc cggcccggag
2101 gcgtcccgga agttcgtgga cacgacctcc gaccactcgg cgtacagctc gtccaggccg
2161 cgcacccaca cccaggccag ggtgttgtcc ggcaccacct ggtcctggac cgcgctgatg
2221 aacagggtca cgtcgtcccg gaccacaccg gcgaagtcgt cctccacgaa gtcccgggag
2281 aacccgagcc ggtcggtcca gaactcgacc gctccggcga cgtcgcgcgc ggtgagcacc
2341 ggaacggcac tggtcaactt ggccatgatg gctcctcctg tcaggagagg aaagagaaga
2401 aggttagtac aattgctata gtgagttgta ttatactatg cagatatact atgccaatga
2461 ttaattgtca aactagggct gcagggttca tagtgccact tttcctgcac tgccccatct
2521 cctgcccacc ctttcccagg catagacagt cagtgactta ccaaactcac aggagggaga
2581 aggcagaagc ttgagacaga cccgcgggac cgccgaactg cgaggggacg tggctagggc
2641 ggcttctttt atggtgcgcc ggccctcgga ggcagggcgc tcggggaggc ctagcggcca
2701 atctgcggtg gcaggaggcg gggccgaagg ccgtgcctga ccaatccgga gcacatagga
2761 gtctcagccc cccgccccaa agcaagggga agtcacgcgc ctgtagcgcc agcgtgttgt
2821 gaaatggggg cttggggggg ttggggccct gactagtcaa aacaaactcc cattgacgtc
2881 aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta
2941 ctgccaaaac cgcatcatca tggtaatagc gatgactaat acgtagatgt actgccaagt
3001 aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt
3061 gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt
3121 ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg
3181 aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc
3241 atttaccgta agttatgtaa cgcctgcagg ttaattaaga acatgtgagc aaaaggccag
3301 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
3361 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
3421 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
3481 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
3541 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
3601 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
3661 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
3721 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
3781 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
3841 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
3901 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
3961 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatggctag ttaattaaca
4021 tttaaatcag cggccgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt
4081 tgtgtgaatc gtaactaaca tacgctctcc atcaaaacaa aacgaaacaa aacaaactag
4141 caaaataggc tgtccccagt gcaagtgcag gtgccagaac atttctctat cgaa
//
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