| 质粒名称: | pMZ0002 |
|---|---|
| 出品公司： | addgene |
| 目录编号: | 102239 |
| 存储实验室: | Axel Brunger |
|
载体骨架基本信息 |
|
| 载体名称: | pPROEX1 |
| 完整质粒大小: | 6914 bp |
| 载体类型: | 细菌细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 30℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | nameN-ethylmaleimide sensitive factor/NSF |
| 物种来源: | Chinese hamster |
| 标签/融合蛋白: | 6x His tag (N terminal on backbone) |
|
文献引用方法 |
|
| 材料和方法部分: | pMZ0002 was a gift from Axel Brunger (Addgene plasmid # 102239 ; http://n2t.net/addgene:102239 ; RRID:Addgene_102239) |
| 参考文献部分: | Mechanistic insights into the recycling machine of the SNARE complex. Zhao M, Wu S, Zhou Q, Vivona S, Cipriano DJ, Cheng Y, Brunger AT. Nature. 2015 Feb 5;518(7537):61-7. doi: 10.1038/nature14148. Epub 2015 Jan 12. 10.1038/nature14148 PubMed 25581794 |
买家导航
pMZ0002载体质粒基本信息
pMZ0002质粒图谱载体图谱和pMZ0002载体序列质粒序列多克隆位点信息
pMZ0002质粒载体简介
pMZ0002质粒序列载体序列
LOCUS pMZ0002 6914 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expresses Chinese hamster NSF in bacteria.
ACCESSION .
VERSION .
KEYWORDS pMZ0002
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6914)
AUTHORS Zhao M, Wu S, Zhou Q, Vivona S, Cipriano DJ, Cheng Y, Brunger AT
TITLE Mechanistic insights into the recycling machine of the SNARE
complex.
JOURNAL Nature. 2015 Feb 5;518(7537):61-7. doi: 10.1038/nature14148. Epub
2015 Jan 12.
PUBMED 25581794
REFERENCE 2 (bases 1 to 6914)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6914
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 11..599
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 500..519
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 753..770
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(847..1275)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(957..978)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 1169..1188
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind complement(1313..1332)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 1473..1495
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
promoter 1573..1650
/gene="lacI (mutant)"
/label=lacIq promoter
/note="In the lacIq allele, a single base change in the
promoter boosts expression of the lacI gene about 10-fold."
CDS 1651..2733
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
primer_bind complement(1670..1689)
/label=LacI-R
/note="LacI, reverse primer"
protein_bind 3002..3018
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 3007..3029
/label=M13/pUC Reverse
/note="In lacZ gene"
CDS 3045..3062
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
CDS 3084..3104
/codon_start=1
/product="tobacco etch virus (TEV) protease recognition and
cleavage site"
/label=TEV site
/translation="ENLYFQG"
primer_bind complement(5408..5425)
/label=pBAD Reverse
/note="For vectors with E. coli araBAD promoter, reverse
primer"
terminator 5578..5664
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 5756..5783
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
promoter 5802..5893
/gene="bla"
/label=AmpR promoter
CDS 5894..6754
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(6112..6131)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
ORIGIN
1 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
61 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
121 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
181 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
241 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
301 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
361 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
421 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
481 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
541 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
601 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
661 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
721 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
781 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcataattt
841 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
901 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
961 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
1021 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
1081 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
1141 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
1201 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
1261 cgctacaggg cgcgtcccat tcgccattca ggctgctatg gtgcactctc agtacaatct
1321 gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat
1381 ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
1441 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
1501 accgtcatca ccgaaacgcg cgaggcagca gatcaattcg cgcgcgaagg cgaagcggca
1561 tgcatttacg ttgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc
1621 ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca
1681 gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt
1741 tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac
1801 cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt
1861 ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg
1921 ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg
1981 gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac
2041 caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc
2101 tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc
2161 gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt
2221 tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt
2281 cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg
2341 caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg
2401 ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta
2461 gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa
2521 caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc
2581 caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg
2641 gcacccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
2701 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg taagttagcg
2761 cgaattgatc tggtttgaca gcttatcatc gactgcacgg tgcaccaatg cttctggcgt
2821 caggcagcca tcggaagctg tggtatggct gtgcaggtcg taaatcactg cataattcgt
2881 gtcgctcaag gcgcactccc gttctggata atgttttttg cgccgacatc ataacggttc
2941 tggcaaatat tctgaaatga gctgttgaca attaatcatc cggtccgtat aatctgtgga
3001 attgtgagcg gataacaatt tcacacagga aacagaccat gggtcatcat catcatcatc
3061 acgattacga tatcccaacg accgaaaacc tgtattttca gggcgcccat atggcgggcc
3121 ggagtatgca agctgcgagg tgccctaccg atgaattgtc cttaagcaac tgtgcagttg
3181 tgagcgagaa ggattaccag tctggacagc atgtgattgt gaggacctcc cccaatcaca
3241 aatacatatt tacactgagg acccatccgt cggtggttcc cggaagcgtt gcattcagct
3301 tacctcagcg aaaatgggct ggactttcta ttgggcagga aatagaagtc gccttgtatt
3361 catttgacaa agccaagcaa tgcatcggca ccatgaccat cgagattgac tttctgcaga
3421 aaaaaaacat cgactccaac ccatatgata ccgacaagat ggcagcagaa ttcatccagc
3481 agttcaacaa ccaggccttc tcagtgggac agcagcttgt ctttagcttt aatgataagc
3541 tcttcggatt actggtgaag gacattgaag caatggaccc tagcatcctg aagggagagc
3601 ctgcatcagg caaaaggcag aagattgaag tcggactggt tgttggaaat agtcaagttg
3661 catttgaaaa agcagaaaat tcatcgctca atcttattgg caaagctaaa accaaggaaa
3721 atcgccagag tattatcaat cctgactgga actttgagaa aatgggaatc ggcggtctgg
3781 ataaggagtt ttcagatatt ttccgacgag catttgcttc tcgggtgttc cctccggaga
3841 ttgtggagca gatgggttgt aaacatgtta aaggcatcct gttgtacggc ccccctggtt
3901 gtggtaagac tctcttggct cgacagattg gcaagatgct gaatgcgaga gaacccaagg
3961 tggtcaatgg gccagaaatc cttaacaaat atgtgggaga atcggaggcg aacattcgta
4021 aactttttgc tgatgccgaa gaggagcaaa ggaggcttgg tgctaacagt ggcttacaca
4081 tcatcatctt tgatgaaatc gatgccatct gcaagcagag agggagcatg gctggcagca
4141 ccggagttca cgacaccgtt gtcaaccagc tgctgtccaa gattgatggc gttgagcagc
4201 tgaacaacat cctggttatt gggatgacca atagaccaga tttgatagat gaggctctcc
4261 ttcgacctgg aagactggaa gttaaaatgg agataggctt gccagatgag aagggtcgac
4321 tgcagatcct tcatatccac acagcaagga tgagaggaca tcagttactg tctgcagatg
4381 tggacattaa ggaactggcc gtggagacta agaatttcag tggcgctgag ctggaaggtc
4441 tggtgcgagc agcacagtcg acggccatga accgacacat aaaggccagt acgaaagtgg
4501 aagtagacat ggagaaagca gagagtctgc aggtgacaag aggagacttc ctggcttctt
4561 tggagaatga tatcaaacca gcttttggca caaaccaaga ggattatgcc agttacatta
4621 tgaatggaat catcaagtgg ggtgaccctg tgacccgtgt cctggatgac ggagagctgc
4681 tggtccagca gactaaaaac agtgaccgta cgcctctggt tagcgtcctt ctggaagggc
4741 cccctcacag cgggaagact gccctagctg caaagatcgc agaggaatcc aactttccct
4801 tcatcaagat ctgctccccc gataagatga ttggcttttc tgagacagcc aagtgtcagg
4861 ccatgaagaa gatctttgat gatgcttaca aatctcaact cagttgtgtg gtggtggatg
4921 acatcgaaag gctacttgat tatgtcccca ttggcccccg gttctccaac ctggtgttgc
4981 aagctcttct tgtgttacta aagaaggcac ctcctcaggg ccgcaagctt ctcatcatcg
5041 ggaccaccag ccgcaaagat gtccttcagg aaatggagat gctgaatgcc ttcagcacca
5101 ccatccacgt gcccaacatt gccacaggag agcagctgct ggaagctttg gagcttctgg
5161 gcaacttcaa agataaggaa cgcaccacaa ttgctcagca agtcaaaggg aagaaggtct
5221 ggataggaat caagaagtta ctaatgttga ttgagatgtc cctgcagatg gaccctgagt
5281 atcgggtgag gaaattcttg gccctcttga gagaagaagg cgctagcccc ctggactttg
5341 attgaggatc cctcgaggca tgcggtacca agcttggctg ttttggcgga tgagagaaga
5401 ttttcagcct gatacagatt aaatcagaac gcagaagcgg tctgataaaa cagaatttgc
5461 ctggcggcag tagcgcggtg gtcccacctg accccatgcc gaactcagaa gtgaaacgcc
5521 gtagcgccga tggtagtgtg gggtctcccc atgcgagagt agggaactgc caggcatcaa
5581 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
5641 aacgctctcc tgagtaggac aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg
5701 cccggagggt ggcgggcagg acgcccgcca taaactgcca ggcatcaaat taagcagaag
5761 gccatcctga cggatggcct ttttgcgttt ctacaaactc ttttgtttat ttttctaaat
5821 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
5881 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
5941 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
6001 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
6061 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
6121 cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc
6181 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
6241 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
6301 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
6361 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
6421 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
6481 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
6541 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
6601 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
6661 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
6721 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
6781 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
6841 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
6901 cgtagaaaag atca
//
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