PCDH-CMV-mCherry-TRIM16

LOCUS      PCDH-CMV-mCherry-TRIM16             8844 bp DNA     circular SYN 04-JUL-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8844)
  AUTHORS   15129015045
  TITLE     Direct Submission
  JOURNAL   Exported Aug 7, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8844
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             234..414
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    458..583
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1076..1309
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1798..1915
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     1904..1953
                     /label=Primer 4
     enhancer        1938..2241
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2242..2445
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2444..2491)
                     /label=Primer 1
     primer_bind     2466..2500
                     /label=Primer 2
     primer_bind     2466..2492
                     /label=Primer 5
     CDS             2496..3203
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     3025..3046
                     /label=mcherry-F
     misc_feature    3204..3232
                     /label=MCS
                     /note="multiple cloning site"
     primer_bind     3218..3257
                     /label=TRIM16-F
     misc_feature    3240..4931
                     /label=TRIM16
     primer_bind     complement(4403..4420)
                     /label=16-seqR0
     primer_bind     complement(4914..4953)
                     /label=TRIM16-R
     misc_feature    4941..5528
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(5412..5423)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5601..5834
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5906..6027
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      6046..6181
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6219..6235)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6243..6259
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6267..6297)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6312..6333
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(6621..7209)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7380..8240)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8241..8345)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8714..8727)
                     /label=Primer 5
     primer_bind     8819..8835
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagttgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     1981 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     2041 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     2101 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     2161 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     2221 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     2281 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     2341 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     2401 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
     2461 cagattctag accgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggaggata
     2521 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     2581 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     2641 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     2701 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     2761 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     2821 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     2881 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     2941 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     3001 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     3061 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     3121 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     3181 gcggcatgga cgagctgtac aagtccggac tcagatctcg agctcaagct tcggaattca
     3241 tggctgagtt ggatctaatg gctccagggc cactgcccag ggccactgct cagcccccag
     3301 cccctctcag cccagactct gggtcaccca gcccagattc tgggtcagcc agcccagtgg
     3361 aagaagagga cgtgggctcc tcggagaagc ttggcaggga gacggaggaa caggacagcg
     3421 actctgcaga gcagggggat cctgctggtg aggggaaaga ggtcctgtgt gacttctgcc
     3481 ttgatgacac cagaagagtg aaggcagtga agtcctgtct aacctgcatg gtgaattact
     3541 gtgaagagca cttgcagccg catcaggtga acatcaaact gcaaagccac ctgctgaccg
     3601 agccagtgaa ggaccacaac tggcgatact gccctgccca ccacagccca ctgtctgcct
     3661 tctgctgccc tgatcagcag tgcatctgcc aggactgttg ccaggagcac agtggccaca
     3721 ccatagtctc cctggatgca gcccgcaggg acaaggaggc tgaactccag tgcacccagt
     3781 tagacttgga gcggaaactc aagttgaatg aaaatgccat ctccaggctc caggctaacc
     3841 aaaagtctgt tctggtgtcg gtgtcagagg tcaaagcggt ggctgaaatg cagtttgggg
     3901 aactccttgc tgctgtgagg aaggcccagg ccaatgtgat gctcttctta gaggagaagg
     3961 agcaagctgc gctgagccag gccaacggta tcaaggccca cctggagtac aggagtgccg
     4021 agatggagaa gagcaagcag gagctggaga ggatggcggc catcagcaac actgtccagt
     4081 tcttggagga gtactgcaag tttaagaaca ctgaagacat caccttccct agtgtttacg
     4141 tagggctgaa ggataaactc tcgggcatcc gcaaagttat cacggaatcc actgtacact
     4201 taatccagtt gctggagaac tataagaaaa agctccagga gttttccaag gaagaggagt
     4261 atgacatcag aactcaagtg tctgccgttg ttcagcgcaa atattggact tccaaacctg
     4321 agcccagcac cagggaacag ttcctccaat atgcgtatga catcacgttt gacccggaca
     4381 cagcacacaa gtatctccgg ctgcaggagg agaaccgcaa ggtcaccaac accacgccct
     4441 gggagcatcc ctacccggac ctccccagca ggttcctgca ctggcggcag gtgctgtccc
     4501 agcagagtct gtacctgcac aggtactatt ttgaggtgga gatcttcggg gcaggcacct
     4561 atgttggcct gacctgcaaa ggcatcgacc ggaaagggga ggagcgcaac agttgcattt
     4621 ccggaaacaa cttctcctgg agcctccaat ggaacgggaa ggagttcacg gcctggtaca
     4681 gtgacatgga gaccccactc aaagctggcc ctttccggag gctcggggtc tatatcgact
     4741 tcccgggagg gatcctttcc ttctatggcg tagagtatga taccatgact ctggttcaca
     4801 agtttgcctg caaattttca gaaccagtct atgctgcctt ctggctttcc aagaaggaaa
     4861 acgccatccg gattgtagat ctgggagagg aacccgagaa gccagcaccg tccttggtgg
     4921 ggactgctcc ctagggatcc aatcaacctc tggattacaa aatttgtgaa agattgactg
     4981 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     5041 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     5101 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     5161 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     5221 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     5281 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga
     5341 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     5401 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     5461 tgcggcctct tccgcgtctc cgccttcgcc ctcagacgag tcggatctcc ctttggccgc
     5521 ctccccgccg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     5581 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaaaat aagatctgct
     5641 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     5701 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     5761 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     5821 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     5881 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     5941 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     6001 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     6061 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     6121 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     6181 ctagactttt gcagagacgg cccaaattcg taatcatggt catagctgtt tcctgtgtga
     6241 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     6301 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     6361 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     6421 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     6481 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     6541 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     6601 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     6661 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     6721 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     6781 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     6841 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     6901 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     6961 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     7021 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     7081 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     7141 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     7201 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     7261 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     7321 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     7381 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     7441 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     7501 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     7561 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     7621 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     7681 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     7741 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     7801 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     7861 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     7921 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     7981 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     8041 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     8101 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     8161 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     8221 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     8281 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     8341 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     8401 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac
     8461 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     8521 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
     8581 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
     8641 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc
     8701 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
     8761 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
     8821 aaaacgacgg ccagtgccaa gctg
//