pCDH-CMV-mCherry-UBAP2L

LOCUS      pCDH-CMV-mCherry-UBAP2L            10413 bp DNA     circular SYN 14-FEB-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10413)
  AUTHORS   15129015045
  TITLE     Direct Submission
  JOURNAL   Exported Aug 2, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..10413
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             234..414
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    458..583
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1076..1309
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1798..1915
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     1904..1953
                     /label=Primer 4
     enhancer        1938..2241
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2242..2445
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             2496..3203
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     misc_feature    3204..3232
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    3219..3257
                     /label=Primer F for UBAP2L in mCherry
     CDS             3240..6500
                     /codon_start=1
                     /label=UBAP2L
                     /translation="MMTSVGTNRARGNWEQPQNQNQTQHKQRPQATAEQIRLAQMISDH
                     NDADFEEKVKQLIDITGKNQDECVIALHDCNGDVNRAINVLLEGNPDTHSWEMVGKKKG
                     VSGQKDGGQTESNEEGKENRDRDRDYSRRRGGPPRRGRGASRGREFRGQENGLDGTKSG
                     GPSGRGTERGRRGRGRGRGGSGRRGGRFSAQGMGTFNPADYAEPANTDDNYGNSSGNTW
                     NNTGHFEPDDGTSAWRTATEEWGTEDWNEDLSETKIFTASNVSSVPLPAENVTITAGQR
                     IDLAVLLGKTPSTMENDSSNLDPSQAPSLAQPLVFSNSKQTAISQPASGNTFSHHSMVS
                     MLGKGFGDVGEAKGGSTTGSQFLEQFKTAQALAQLAAQHSQSGSTTTSSWDMGSTTQSP
                     SLVQYDLKNPSDSAVHSPFTKRQAFTPSSTMMEVFLQEKSPAVATSTAAPPPPSSPLPS
                     KSTSAPQMSPGSSDNQSSSPQPAQQKLKQQKKKASLTSKIPALAVEMPGSADISGLNLQ
                     FGALQFGSEPVLSDYESTPTTSASSSQAPSSLYTSTASESSSTISSNQSQESGYQSGPI
                     QSTTYTSQNNAQGPLYEQRSTQTRRYPSSISSSPQKDLTQAKNGFSSVQATQLQTTQSV
                     EGATGSAVKSDSPSTSSIPPLNETVSAASLLTTTNQHSSSLGGLSHSEEIPNTTTTQHS
                     STLSTQQNTLSSSTSSGRTSTSTLLHTSVESEANLHSSSSTFSTTSSTVSAPPPVVSVS
                     SSLNSGSSLGLSLGSNSTVTASTRSSVATTSGKAPPNLPPGVPPLLPNPYIMAPGLLHA
                     YPPQVYGYDDLQMLQTRFPLDYYSIPFPTPTTPLTGRDGSLASNPYSGDLTKFGRGDAS
                     SPAPATTLAQPQQNQTQTHHTTQQTFLNPALPPGYSYTSLPYYTGVPGLPSTFQYGPAV
                     FPVAPTSSKQHGVNVSVNASATPFQQPSGYGSHGYNTGVSVTSSNTGVPDISGSVYSKT
                     QQSFEKQGFHSGTPAASFNLPSALGSGGPINPATAAAYPPAPFMHILTPHQQPHSQILH
                     HHLQQDGQTGSGQRSQTSSIPQKPQTNKSAYNSYSWGAN"
     primer_bind     complement(5864..5881)
                     /label=YZY-P764
     misc_feature    complement(6483..6521)
                     /label=Primer R for UBAP2L in mCherrt
     misc_feature    6510..7097
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(6981..6992)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7170..7403
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7475..7596
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      7615..7750
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7788..7804)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7812..7828
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7836..7866)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7881..7902
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(8190..8778)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8949..9809)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9810..9914)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10388..10404
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagttgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     1981 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     2041 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     2101 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     2161 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     2221 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     2281 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     2341 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     2401 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
     2461 cagattctag accgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggaggata
     2521 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     2581 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     2641 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     2701 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     2761 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     2821 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     2881 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     2941 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     3001 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     3061 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     3121 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     3181 gcggcatgga cgagctgtac aagtccggac tcagatctcg agctcaagct tcggaattca
     3241 tgatgacatc ggtgggcact aaccgagccc ggggaaactg ggaacaacct caaaaccaaa
     3301 accagacaca gcacaagcag cggccacagg ccactgcaga acaaattaga cttgcacaga
     3361 tgatttcgga ccataatgat gctgactttg aggagaaggt gaaacaattg attgatatta
     3421 caggcaagaa ccaggatgaa tgtgtgattg ctttgcatga ctgcaatgga gatgtcaaca
     3481 gagctatcaa tgttcttctg gaaggaaacc cagacacgca ttcctgggag atggtcggga
     3541 agaagaaggg agtctcaggc cagaaggatg gtggccagac ggaatccaat gaggaaggca
     3601 aagaaaatcg agaccgggac agagactata gtcggcgacg tggtgggcca ccaagacggg
     3661 ggagaggtgc cagccgtgga cgagagtttc gaggtcagga aaatggattg gatggcacca
     3721 agagtggagg gccttctgga agaggaacag aaagaggcag aaggggccgt ggccgaggca
     3781 gaggtggctc tggtaggcga ggaggaaggt tttctgctca aggaatggga acctttaacc
     3841 cagctgatta tgcagagcca gccaatactg atgataacta tggcaatagc agcggcaata
     3901 cgtggaacaa cactggccac tttgaaccag atgatgggac gagtgcatgg aggactgcaa
     3961 cagaggagtg ggggactgaa gattggaatg aagatctttc tgagaccaag atcttcactg
     4021 cctctaatgt gtcttcagtg cctctgcctg cggagaatgt gacaatcact gctggtcaga
     4081 gaattgacct tgctgttctg ctggggaaga caccatctac aatggagaat gattcatcta
     4141 atctggatcc gtctcaggct ccttctctgg cccagcctct ggtgttcagt aattcgaagc
     4201 agactgccat atcacagcct gcttcaggga acacattttc tcatcacagt atggtgagca
     4261 tgttagggaa aggatttggt gatgtcggtg aagctaaagg cggcagtact acaggctccc
     4321 agttcttgga gcaattcaag actgcccaag ccctggctca gttggcagct cagcattctc
     4381 agtctggaag caccaccacc tcctcttggg acatgggctc gacgacacaa tccccatcac
     4441 tggtgcagta tgatttgaag aacccaagtg attcagcagt gcacagcccc tttacaaagc
     4501 gccaggcttt taccccatct tcaaccatga tggaggtgtt ccttcaggag aagtcacctg
     4561 cagtggctac ctccacagct gcacctccac ctccgtcttc tcctctgcca agcaaatcca
     4621 catcggctcc acagatgtcg cctggatctt cagacaacca gtcctctagc cctcagccgg
     4681 ctcagcagaa actgaaacag cagaagaaaa aagcctcctt gacttctaag attcctgctc
     4741 tggctgtgga gatgcctggc tcagcagata tctcagggct aaacctgcag tttggggcat
     4801 tgcagtttgg gtcagagcct gtcctttctg attatgagtc cacccccacc acgagcgcct
     4861 cttcaagcca ggctccaagt agcctgtata ccagcacggc cagtgaatca tcctctacaa
     4921 tttcatctaa ccagagtcag gagtctggtt atcagagcgg cccaattcag tcgacaacct
     4981 atacctccca aaataatgct cagggccctc tttatgaaca gagatccaca cagactcggc
     5041 ggtaccccag ctccatctct tcatcacccc aaaaggacct gactcaggca aagaatggct
     5101 tcagttctgt gcaggccacg cagttacaga ccacacaatc tgttgaaggt gctacaggct
     5161 ctgcagtgaa atctgattca ccttccactt ctagcatccc ccctctcaat gaaacggtat
     5221 ctgcagcttc cttactgacg acaaccaatc agcattcatc ctccttgggt ggcttgagcc
     5281 acagtgagga gattccaaat actaccacca cacaacacag cagcacgtta tctacgcagc
     5341 agaataccct ttcatcatca acatcttctg ggcgcacttc gacatccact cttttgcaca
     5401 caagtgtgga gagtgaggcg aatctccatt cttcctccag cactttttcc accacatcca
     5461 gcacagtctc tgcacctccc ccagtggtca gtgtctcctc cagtctcaat agtggcagta
     5521 gcctgggcct cagcctaggc agcaactcca ctgtcacagc ctcgactcga agctcagttg
     5581 ctacgacttc aggaaaagct cctcccaacc tccctcctgg ggtcccgccg ttgttgccta
     5641 atccgtatat tatggctcca gggctgttac atgcctaccc gccacaagta tatggttatg
     5701 atgacttgca gatgcttcag acaagatttc cattggatta ctacagcatc ccatttccca
     5761 cacccactac tccgctgact gggagggatg gtagcctggc cagcaaccct tattctggtg
     5821 acctcacaaa gttcggccgt ggggatgcct cctccccagc cccggccaca accttggccc
     5881 aaccccaaca gaaccagacg cagactcacc ataccacgca gcagacattc ctgaacccgg
     5941 cgctgcctcc tggctacagt tacaccagcc tgccatacta tacaggggtc ccgggcctcc
     6001 ccagcacctt ccagtatggg cctgctgtgt tccctgtggc tcctacctct tccaagcagc
     6061 atggtgtgaa tgtcagtgtg aatgcatcgg ccaccccttt ccaacagccg agtggatatg
     6121 ggtctcatgg atacaacact ggtgtttcag tcacctccag taacacgggc gtgccagata
     6181 tctcgggttc tgtgtactcc aaaacccagc agtcctttga gaaacaaggt tttcattccg
     6241 gtactcctgc tgcttccttc aacttgcctt cagccctagg aagtgggggc cccatcaatc
     6301 cggccacagc tgctgcctac ccacctgccc cctttatgca cattctgacc ccccatcagc
     6361 agccgcattc tcagatcctt caccatcacc tgcagcagga tggccagacg ggcagcgggc
     6421 aacgtagcca gaccagctcc atcccgcaga agccccagac caacaagtct gcctacaaca
     6481 gctacagctg gggggccaac tagggatcca atcaacctct ggattacaaa atttgtgaaa
     6541 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     6601 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     6661 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     6721 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     6781 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     6841 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     6901 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     6961 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     7021 tgccggctct gcggcctctt ccgcgtctcc gccttcgccc tcagacgagt cggatctccc
     7081 tttggccgcc tccccgccgg tacctttaag accaatgact tacaaggcag ctgtagatct
     7141 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaaaata
     7201 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     7261 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     7321 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     7381 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     7441 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     7501 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     7561 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     7621 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     7681 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     7741 tttggaggcc tagacttttg cagagacggc ccaaattcgt aatcatggtc atagctgttt
     7801 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     7861 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     7921 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     7981 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     8041 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     8101 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     8161 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     8221 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     8281 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     8341 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     8401 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     8461 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     8521 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     8581 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     8641 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     8701 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     8761 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     8821 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     8881 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     8941 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     9001 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     9061 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     9121 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     9181 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     9241 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     9301 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     9361 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     9421 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     9481 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     9541 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     9601 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     9661 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     9721 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     9781 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     9841 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     9901 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     9961 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc
    10021 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
    10081 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
    10141 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
    10201 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc
    10261 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
    10321 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
    10381 cgacgttgta aaacgacggc cagtgccaag ctg
//