| 质粒名称: | pAAVLINK-EF1a-HA-CHD9-A |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001739 |
| 产品用途: | 3' AAVLINK plasmid for CHD9 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | CHD9 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-CHD9-A
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-CHD9-A载体质粒基本信息
pAAVLINK-EF1a-HA-CHD9-A质粒图谱载体图谱和pAAVLINK-EF1a-HA-CHD9-A载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-CHD9-A质粒载体简介
pAAVLINK-EF1a-HA-CHD9-A质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-CHD9-A 6759 bp DNA circular SYN 22-JUL-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6759)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 5, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6759
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..141
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..141
/label=AAV2 ITR
promoter 169..711
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 198..409
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 422..690
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 516..521
/label=SDS
CDS 727..753
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
misc_feature 760..3899
/label=CHD9
misc_feature 1983..1988
/label=SDS
misc_feature 1991..1996
/label=SDS
misc_feature 3277..3282
/label=SDS
intron 3900..3940
/label=Splicing Donor
misc_feature 3944..3977
/label=loxP-JT1571
repeat_region 4022..4162
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4022..4151
/label=AAV2 ITR
rep_origin 4237..4692
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4974..5078
/gene="bla"
/label=AmpR promoter
CDS 5079..5939
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6110..6698
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
121 actccatcac taggggttcc tgcggccgca cgcgtgtcga ctctagagga tctgcgatcg
181 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
241 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
301 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
361 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
421 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
481 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
541 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
601 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
661 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta ccgtacggcc
721 accatgtacc cttatgacgt acctgactat gctggatcta tgacagatcc aatgatggac
781 ttttttgatg atgccaatct ttttggtgag accttagaag gtttgtcaga tgatgcattt
841 gtacaaccag gacctgtttc actagttgat gaattgaatt tgggtgcaga atttgaaccg
901 ttgcacatag attcactgaa ccatgttcaa ggtactccaa cacatcagaa gatgactgat
961 tttgaacagt taaatcaatt tgattcaata aaatttcacc atgttaatca atcttttggt
1021 agcccagctg aacatgtgtt atctccacac tctcagttta attgttctcc aatccatccc
1081 caaaaccaac ccaatggttt gtttccagat gtatcagatg gcagtccaat gtggggccat
1141 cagacagcta ctaccatttc aaatcaaaat ggatctcctt ttcaccaaca aggacattca
1201 cactctatgc atcaaaataa aagctttgtg gcacaccatg actttgcctt atttcaggcc
1261 aatgaacaac aaacacagtg tacttcacta cgctcacaac aaaacagaaa taatctcaac
1321 ccagggcaga attctcttag ccagtctaaa aattttatga atgtttctgg tccacataga
1381 gtcaatgtta accacccacc acagatgact aatgcatcta attcacaaca gtctatttca
1441 atgcagcaat tttctcaaac gtcaaatcct tcagcacact tccacaagtg tagcagtcat
1501 caagaaggaa attttaatgg accttcccca aatatgactt cttgttctgt cagtaattca
1561 cagcaatttt cttcacatta ttccttttcc agtaatcata tatcaccaaa cagtctactt
1621 cagtcctctg cagttcttgc atctaatcat acaaatcaga ctttatctga ttttactgga
1681 agtaattcct tttcacctca tagaggaatc aagcaagaat ctactcagca tatcctaaac
1741 cccaatacat cattgaattc aaataatttc caaatattgc attcatcaca tcctcagggt
1801 aattatagca attcaaaatt atctcctgtg cacatgaact tcccagatcc tgttgactca
1861 ggaactcaaa tgggccattt caatgatcat gtagaaacta atggcttttc atctttagaa
1921 gagaatttac ttcatcaagt ggaatctcaa actgagccat tcacaggact tgaccccgag
1981 gacctccttc aggagggtct tcttcctcac tttgatgagt caacattcgg acaagataat
2041 tcaagtcaca ttttagatca tgaccttgat cggcagttta cttcgcatct ggtaacacgg
2101 ccttctgata tggctcagac tcagttgcaa agtcaggctc ggagttggca ttcatcattt
2161 tctaatcatc agcatttaca tgacagaaat cacctatgtt tacagcgaca gcctccatct
2221 tccaagaaga gcgatggttc tgggacatat actaagttgc agaataccca ggtgagggtc
2281 atgtctgaga agaagcagag aaaaaaggtg gaatcagaaa gcaagcaaga aaaggctaat
2341 cgtataatat cagaggccat agcaaaagca aaggagcgtg gggaacgcaa tattccacga
2401 gtaatgagcc ctgaaaactt tcctactgct tcagttgaag gaaaagagga aaagaaaggt
2461 agaaggatga aatccaagcc aaaggacaaa gacagcaaaa aaacaaaaac atgttctaag
2521 ttaaaagaga agacaaaaat tggcaaactc attattacat tgggtaagaa acaaaaaaga
2581 aagaatgagt cttcagatga aatatctgat gcagaacaga tgccacagca tacattaaaa
2641 gatcaagact ctcaaaaaag aagatcaaat cgacaaatta aaagaaaaaa atacgcagaa
2701 gatatagaag ggaagcaatc tgaagaagag gttaaaggtt ctatgaaaat aaaaaagaat
2761 tcagctcctt tacctggtga acagccttta caattgtttg tggagaatcc gagtgaagaa
2821 gatgctgcaa ttgtagacaa aattctatct tctagaaccg taaaaaagga aatatcacct
2881 ggagtgatga ttgatacaga agaatttttt gtaaaataca agaattactc ctatcttcac
2941 tgtgagtggg ccacagaaga gcagcttttg aaagataaaa ggatccagca gaaaatcaaa
3001 cgattcaaat tgagacaagc acaaagagca catttttttg cagacatgga agaagaacca
3061 tttaacccag actacgttga agtagacaga gtattagaag tctctttttg tgaagataag
3121 gatactggtg agcctgttat ttactactta gtaaaatggt gctcattgcc atatgaagat
3181 agtacttggg aactaaaaga agatgtagat cttgcaaaaa tagaagagtt tgaacaactg
3241 caagcttcaa ggcctgacac aagacgtttg gaccgtcctc cttctaatat ttggaagaaa
3301 atagatcaat ccagggacta taaaaatggc aatcaactca gggaatatca actggaagga
3361 ctcaactggc tcttgttcaa ttggtacaat agacgaaact gcatcttagc agatgaaatg
3421 ggtcttggca aaactattca atcaattaca ttcctctatg aaatccttct gactggtata
3481 agaggacctt tcctgattat tgctccactt tctactattg caaactggga gagagaattt
3541 cgtacgtgga ctgatattaa cgttgtggtt tatcatggga gcctgattag cagacaaatg
3601 atacagcaat acgagatgta cttcagggat tcacaggggc gtatcattcg aggagcttac
3661 agattccaag ccatcatcac cacttttgaa atgattcttg gaggctgtgg agagcttaat
3721 gcaattgaat ggcgatgtgt gattattgat gaagcacata ggttaaaaaa taaaaattgt
3781 aaactcttag agggcctgaa actcatgaat ctggaacaca aggtgctttt gactggcacc
3841 cctctccaaa atacagttga agaactattt agtcttcttc actttcttga acccttaagg
3901 taagtatcaa ggttacaaga caggtttaag gagaccaata agctattatt cgtatagcat
3961 acattatacg aagttattaa gttatggaga ccggtgtggc acgtgcggac cgagcggccg
4021 caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag
4081 gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag
4141 cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc
4201 ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa
4261 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
4321 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
4381 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
4441 aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
4501 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
4561 cactcaaccc tatctcgggc tattcttttg atttataagg gattttgccg atttcggcct
4621 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
4681 cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
4741 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
4801 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
4861 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
4921 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
4981 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
5041 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
5101 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
5161 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
5221 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
5281 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
5341 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
5401 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
5461 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
5521 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
5581 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
5641 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
5701 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
5761 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
5821 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
5881 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
5941 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
6001 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
6061 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
6121 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
6181 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
6241 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
6301 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
6361 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
6421 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
6481 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
6541 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
6601 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
6661 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
6721 tacggttcct ggccttttgc tggccttttg ctcacatgt
//
