| 质粒名称: | FUW-tetO-EGFP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 84041 |
| 存储实验室: | Stefano Piccolo |
|
载体骨架基本信息 |
|
| 载体名称: | FUW |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 8482 bp |
| 完整质粒大小: | 10463 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Enhanced Green Fluorescence Protein, Puromycin resistance gene,EGFP-IRES-Puro |
| 物种来源: | -- |
| 插入大小: | 1947 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | TRE-CMV |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | EcoRI(克隆期间销毁) |
| 3' 克隆位点: | EcoRI(克隆期间销毁) |
| 5' 测序引物 | LNCX |
| 3' 测序引物 | pBluescriptKS |
|
文献引用方法 |
|
| 材料和方法部分: | FUW-tetO-EGFP was a gift from Stefano Piccolo (Addgene plasmid # 84041 ; http://n2t.net/addgene:84041 ; RRID:Addgene_84041) |
| 参考文献部分: | Induction of Expandable Tissue-Specific Stem/Progenitor Cells through Transient Expression of YAP/TAZ. Panciera T, Azzolin L, Fujimura A, Di Biagio D, Frasson C, Bresolin S, Soligo S, Basso G, Bicciato S, Rosato A, Cordenonsi M, Piccolo S. Cell Stem Cell. 2016 Sep 9. pii: S1934-5909(16)30256-9. doi: 10.1016/j.stem.2016.08.009. 10.1016/j.stem.2016.08.009 PubMed 27641305 |
买家导航
FUW-tetO-EGFP载体质粒基本信息
FUW-tetO-EGFP质粒图谱载体图谱和FUW-tetO-EGFP载体序列质粒序列多克隆位点信息
FUW-tetO-EGFP质粒载体简介
FUW-tetO-EGFP质粒序列载体序列
LOCUS FUW-tetO-EGFP 10457 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION 3rd generation lentiviral transfer plasmid. Expresses EGFP and
puromycin resistance in mammalian cells under the control of a
doxycycline-inducible promoter.
ACCESSION .
VERSION .
KEYWORDS FUW-tetO-EGFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10457)
AUTHORS Panciera T, Azzolin L, Fujimura A, Di Biagio D, Frasson C, Bresolin
S, Soligo S, Basso G, Bicciato S, Rosato A, Cordenonsi M, Piccolo S
TITLE Induction of Expandable Tissue-Specific Stem/Progenitor Cells
through Transient Expression of YAP/TAZ.
JOURNAL Cell Stem Cell. 2016 Sep 9. pii: S1934-5909(16)30256-9. doi:
10.1016/j.stem.2016.08.009.
PUBMED 27641305
REFERENCE 2 (bases 1 to 10457)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10457
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 27..406
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 408..606
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 561..581
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 624..804
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 851..976
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1469..1702
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2229..2346
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
protein_bind 2399..2669
/label=tetracycline response element
/bound_moiety="tetracycline repressor TetR"
/note="contains seven copies of the tetracycline operator
tetO"
promoter 2702..2740
/label=minimal CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 2737..2761
/label=LNCX
/note="Human CMV promoter, forward primer"
regulatory 2895..2904
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 2901..3620
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(2946..2967)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(3207..3226)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 3554..3575
/label=EGFP-C
/note="EGFP, forward primer"
misc_feature 3660..4246
/label=IRES2
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(3827..3844)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 4054..4073
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 4247..4846
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(4247..4266)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 4743..4763
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature 4938..5526
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(4991..5011)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5409..5420)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(5529..5545)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5530..5546)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 6051..6231
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(6257..6274)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 6263..6487
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 6533..6961
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6620..6639)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6830..6851
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(6970..6990)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 6975..7304
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 7155..7290
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 7217..7236
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 7352..7399
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 7418..7792
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 7922..8043
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7959..7978)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 8013..8032
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(8092..8108)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8092..8108)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(8105..8127)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 8116..8132
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(8140..8170)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8185..8206
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(8323..8340)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8494..9082)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8574..8593)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(9253..10113)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9876..9895
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(10114..10218)
/gene="bla"
/label=AmpR promoter
primer_bind complement(10293..10312)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
601 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
661 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
721 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
781 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
841 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
901 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
961 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
1021 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
1081 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
1141 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
1201 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
1261 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
1321 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
1381 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
1441 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
1501 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
1561 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
1621 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
1681 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
1741 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
1801 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
1861 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
1921 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
1981 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
2041 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
2101 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
2161 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
2221 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
2281 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
2341 aattttcggg tttattacag ggacagcaga gatccagttt ggttatcgag tttaccactc
2401 cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa
2461 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
2521 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
2581 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
2641 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagctcggt acccgggtcg
2701 aggtaggcgt gtacggtggg aggcctatat aagcagagct cgtttagtga accgtcagat
2761 cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag
2821 cctccgcggc cccgaattgg ccgcaacaag atctgcgatc taagtaagct tggcattccg
2881 gtactgttgg taaagccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
2941 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
3001 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
3061 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
3121 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
3181 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
3241 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
3301 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
3361 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
3421 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
3481 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
3541 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
3601 tggacgagct gtacaagtaa agcggccaac tcgacgttat tcccttcgat cgaattccgc
3661 ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt
3721 gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg
3781 aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga
3841 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa
3901 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc
3961 tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac
4021 gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag
4081 gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc
4141 acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg
4201 gacgtggttt tcctttgaaa aacacgatga taatatggcc acaaccatga ccgagtacaa
4261 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
4321 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
4381 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
4441 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
4501 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
4561 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
4621 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
4681 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
4741 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
4801 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacgcc cgccccacga
4861 cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca tcgatttcga acccaattcg
4921 atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
4981 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
5041 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
5101 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
5161 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
5221 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
5281 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
5341 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
5401 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
5461 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
5521 ccccgcatcg ataccgtcga cctcgagacc tagaaaaaca tggagcaatc acaagtagca
5581 atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg
5641 gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
5701 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
5761 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact
5821 acacaccagg gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac
5881 cagttgagca agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc
5941 ctgtgagcct gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca
6001 gccgcctagc atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct
6061 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
6121 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
6181 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agggcccgtt
6241 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
6301 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
6361 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
6421 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
6481 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
6541 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
6601 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
6661 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
6721 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
6781 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
6841 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
6901 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
6961 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
7021 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
7081 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
7141 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
7201 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
7261 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
7321 gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat
7381 cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt
7441 tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg
7501 gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct
7561 gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt
7621 gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga
7681 cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct
7741 gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct
7801 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
7861 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
7921 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
7981 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
8041 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
8101 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
8161 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
8221 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
8281 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
8341 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
8401 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
8461 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
8521 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
8581 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
8641 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
8701 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
8761 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
8821 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
8881 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
8941 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
9001 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
9061 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
9121 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
9181 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
9241 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
9301 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
9361 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
9421 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
9481 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
9541 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
9601 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
9661 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
9721 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
9781 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
9841 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
9901 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
9961 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
10021 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
10081 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
10141 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
10201 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg
10261 agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
10321 agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt
10381 taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg
10441 cgttttgcgc tgcttcg
//
