pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ATP7A-FLAG

LOCUS      pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ATP7A-FLAG             6707 bp DNA     circular SYN 25-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6707)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 12, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6707
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..3420
                     /codon_start=1
                     /gene="ATP7A"
                     /gene_synonym="DSMAX; HMNX; MK; MNK; SMAX3"
                     /product="copper-transporting ATPase 1 isoform 1"
                     /EC_number="7.2.2.8"
                     /label=ATP7A
                     /note="isoform 1 is encoded by transcript variant 1;
                     Menkes disease-associated protein; copper pump 1; 
                     copper-transporting ATPase 1; Cu++-transporting P-type 
                     ATPase; ATPase, Cu++ transporting, alpha polypeptide"
                     /db_xref="CCDS:CCDS35339.1"
                     /db_xref="GeneID:538"
                     /db_xref="HGNC:HGNC:869"
                     /db_xref="MIM:300011"
                     /protein_id="NP_000043.4"
                     /translation="EAMPVAKKPGSTVIAGSINQNGSLLICATHVGADTTLSQIVKLVE
                     EAQTSKAPIQQFADKLSGYFVPFIVFVSIATLLVWIVIGFLNFEIVETYFPGYNRSISR
                     TETIIRFAFQASITVLCIACPCSLGLATPTAVMVGTGVGAQNGILIKGGEPLEMAHKVK
                     VVVFDKTGTITHGTPVVNQVKVLTESNRISHHKILAIVGTAESNSEHPLGTAITKYCKQ
                     ELDTETLGTCIDFQVVPGCGISCKVTNIEGLLHKNNWNIEDNNIKNASLVQIDASNEQS
                     STSSSMIIDAQISNALNAQQYKVLIGNREWMIRNGLVINNDVNDFMTEHERKGRTAVLV
                     AVDDELCGLIAIADTVKPEAELAIHILKSMGLEVVLMTGDNSKTARSIASQVGITKVFA
                     EVLPSHKVAKVKQLQEEGKRVAMVGDGINDSPALAMANVGIAIGTGTDVAIEAADVVLI
                     RNDLLDVVASIDLSRKTVKRIRINFVFALIYNLVGIPIAAGVFMPIGLVLQPWMGSAAM
                     AASSVSVVLSSLFLKLYRKPTYESYELPARSQIGQKSPSEISVHVGIDDTSRNSPKLGL
                     LDRIVNYSRASINSLLSDKRSLNSVVTSEPDKHSLLVGDFREDDDTAL"
     misc_feature    2792..2797
                     /label=SDS
     CDS             3427..3492
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3496..3927
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3640..3645
                     /label=SDS
     repeat_region   3970..4110
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    3970..4099
                     /label=AAV2 ITR
     repeat_region   3971..4111
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4185..4640
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4922..5026
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5027..5887
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6058..6646
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacagggga ggcaatgcct
     1561 gtggctaaga aacctggcag cacagtgatt gctggttcca ttaaccagaa cgggtcactg
     1621 cttatctgcg caacacatgt tggagcagac acaacccttt ctcaaattgt caaacttgtg
     1681 gaagaggcac aaacatcaaa ggctcctatc cagcagtttg cagacaaact cagtggctat
     1741 tttgttcctt ttattgtttt tgtttccatt gccaccctct tggtatggat tgtaattgga
     1801 tttctgaatt ttgaaattgt ggaaacctac tttcctggct acaatagaag tatctcccga
     1861 acagaaacga taatacgatt tgctttccaa gcctctatca cagttctgtg tattgcatgt
     1921 ccctgttcac tgggactggc cactccaact gctgtgatgg tgggtacagg agtaggtgct
     1981 caaaatggca tactaataaa aggtggagag ccattggaga tggctcataa ggtaaaggta
     2041 gtggtatttg ataagactgg aaccattact cacggaaccc cagtggtgaa tcaagtaaag
     2101 gttctaactg aaagtaacag aatatcacac cataaaatct tggccattgt gggaactgct
     2161 gaaagtaaca gtgaacaccc tctaggaaca gccataacca aatattgcaa acaggagctg
     2221 gacactgaaa ccttgggtac ctgcatagat ttccaggttg tgccaggctg tggtattagc
     2281 tgtaaagtca ccaatattga aggcttgcta cataagaata actggaatat agaggacaat
     2341 aatattaaaa atgcatccct ggttcaaatt gatgccagta atgaacagtc atcaacttcg
     2401 tcttccatga ttattgatgc ccagatctca aatgctctta atgctcagca gtataaagtc
     2461 ctcattggta accgggagtg gatgattaga aatggtcttg tcattaataa cgatgtaaat
     2521 gatttcatga ctgaacatga gagaaaaggt cggactgctg tattagtagc agttgatgat
     2581 gagctgtgtg gcttgatagc cattgcagac acagtgaagc ctgaagcaga actggctatc
     2641 catattctga aatctatggg cttagaagta gttctgatga ctggagacaa cagtaaaaca
     2701 gctagatcta ttgcttctca ggttggcatt actaaggtgt ttgctgaagt tctaccttct
     2761 cacaaggttg ctaaagtgaa gcaacttcaa gaggagggga aacgggtagc aatggtggga
     2821 gatggaatca atgactcccc agctctggca atggctaatg tgggaattgc tattggcaca
     2881 ggcacagatg tagccattga agcagctgat gtggttttga taaggaatga tcttctggat
     2941 gtagtggcaa gtattgactt atcaagaaag acagtcaaga ggattcggat aaattttgtc
     3001 tttgctctaa tttataatct ggttggaatt cccatagctg ctggagtttt tatgcccatt
     3061 ggtttggttt tgcagccctg gatgggatct gcagcaatgg ctgcttcatc tgtttctgta
     3121 gtactttctt ctctcttcct taaactttac aggaaaccaa cttacgagag ttatgaactg
     3181 cctgcccgga gccagatagg acagaagagt ccttcagaaa tcagcgttca tgttggaata
     3241 gatgatacct caaggaattc tcctaaactg ggtttgctgg accggattgt taattatagc
     3301 agagcctcta taaactcact actgtctgat aaacgctccc taaacagtgt tgttaccagt
     3361 gaacctgaca agcactcact cctggtggga gacttcaggg aagatgatga cactgcatta
     3421 tgtacagact acaaagacca tgacggtgat tataaagatc atgacatcga ctacaaggat
     3481 gacgatgaca agtaactcga gataatcaac ctctggatta caaaatttgt gaaagattga
     3541 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     3601 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     3661 tagttcttgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     3721 ggctgttggg cactgacaat tccgtggtgt ttatttgtga aatttgtgat gctattgctt
     3781 tatttgtaac catctagctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     3841 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     3901 cagggggaga tgtgggaggt tttttaaaga tctggagacc ggtgtggcac gtgcggaccg
     3961 agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc
     4021 tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag
     4081 tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc
     4141 atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg
     4201 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     4261 cttagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     4321 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     4381 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac
     4441 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     4501 tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga ttttgccgat
     4561 ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     4621 aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata
     4681 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     4741 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     4801 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     4861 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     4921 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     4981 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     5041 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     5101 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     5161 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     5221 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     5281 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     5341 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     5401 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     5461 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     5521 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     5581 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     5641 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     5701 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     5761 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     5821 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     5881 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     5941 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     6001 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     6061 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     6121 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     6181 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     6241 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     6301 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6361 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     6421 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     6481 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     6541 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     6601 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     6661 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt
//