pAAVLINK-EF1alpha-HA-5'-OTOF

LOCUS       pAAVLINK-EF1alpha-HA-5'-OTOF              7103 bp DNA     circular SYN 25-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7103)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 15, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7103
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        158..700
                     /label=EF1-alpha
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
     promoter        187..398
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             411..679
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     misc_feature    505..510
                     /label=SDS
     CDS             716..742
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             749..4243
                     /codon_start=1
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /product="otoferlin isoform a"
                     /label=OTOF
                     /note="isoform a is encoded by transcript variant 
                     1;fer-1-like protein 2; fer-1-like family member 2"
                     /db_xref="GI:34740331"
                     /db_xref="CCDS:CCDS1725.1"
                     /db_xref="GeneID:9381"
                     /db_xref="HGNC:HGNC:8515"
                     /db_xref="MIM:603681"
                     /protein_id="NP_919224.1"
                     /translation="MALLIHLKTVSELRGRGDRIAKVTFRGQSFYSRVLENCEDVADFD
                     ETFRWPVASSIDRNEMLEIQVFNYSKVFSNKLIGTFRMVLQKVVEESHVEVTDTLIDDN
                     NAIIKTSLCVEVRYQATDGTVGSWDDGDFLGDESLQEEEKDSQETDGLLPGSRPSSRPP
                     GEKSFRRAGRSVFSAMKLGKNRSHKEEPQRPDEPAVLEMEDLDHLAIRLGDGLDPDSVS
                     LASVTALTTNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGD
                     DKKYTSMKESTNCPYYNEYFVFDFHVSPDVMFDKIIKISVIHSKNLLRSGTLVGSFKMD
                     VGTVYSQPEHQFHHKWAILSDPDDISSGLKGYVKCDVAVVGKGDNIKTPHKANETDEDD
                     IEGNLLLPEGVPPERQWARFYVKIYRAEGLPRMNTSLMANVKKAFIGENKDLVDPYVQV
                     FFAGQKGKTSVQKSSYEPLWNEQVVFTDLFPPLCKRMKVQIRDSDKVNDVAIGTHFIDL
                     RKISNDGDKGFLPTLGPAWVNMYGSTRNYTLLDEHQDLNEGLGEGVSFRARLLLGLAVE
                     IVDTSNPELTSSTEVQVEQATPISESCAGKMEEFFLFGAFLEASMIDRRNGDKPITFEV
                     TIGNYGNEVDGLSRPQRPRPRKEPGDEEEVDLIQNASDDEAGDAGDLASVSSTPPMRPQ
                     VTDRNYFHLPYLERKPCIYIKSWWPDQRRRLYNANIMDHIADKLEEGLNDIQEMIKTEK
                     SYPERRLRGVLEELSCGCCRFLSLADKDQGHSSRTRLDRERLKSCMRELENMGQQARML
                     RAQVKRHTVRDKLRLCQNFLQKLRFLADEPQHSIPDIFIWMMSNNKRVAYARVPSKDLL
                     FSIVEEETGKDCAKVKTLFLKLPGKRGFGSAGWTVQAKVELYLWLGLSKQRKEFLCGLP
                     CGFQEVKAAQGLGLHAFPPVSLVYTKKQAFQLRAHMYQARSLFAADSSGLSDPFARVFF
                     INQSQCTEVLNETLCPTWDQMLVFDNLELYGEAHELRDDPPIIVIEIYDQDSMGKADFM
                     GRTFAKPLVKMADEVYCPPRFPPQLEYYQIYRGNATAGDLLAAFELLQIGPAGKADLPP
                     INGPVDVDRGPIMPVPMGIRPVLSKYRVEVLFWGLRDLKRVNLAQVDRPRVDIECAGK"
     misc_feature    1311..1316
                     /label=SDS
     misc_feature    3375..3380
                     /label=SDS
     misc_feature    3549..3554
                     /label=SDS
     intron          4244..4284
                     /label=Splicing Donor
     misc_feature    4288..4321
                     /label=lox2272-JT1571
     repeat_region   4366..4506
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4367..4507
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4581..5036
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5318..5422
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5423..6283
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6454..7042
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
      181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
      241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
      301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
      361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
      421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
      481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
      541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
      601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
      661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
      721 ttatgacgta cctgactatg ctggatctat ggccttgctc atccacctca agacagtctc
      781 ggagctgcgg ggcaggggcg accggatcgc caaagtgact ttccgagggc aatccttcta
      841 ctctcgggtc ctggagaact gtgaggatgt ggctgacttt gatgagacat ttcggtggcc
      901 ggtggccagc agcatcgaca gaaatgagat gctggagatt caggttttca actacagcaa
      961 agtcttcagc aacaagctca tcgggacctt ccgcatggtg ctgcagaagg tggtagagga
     1021 gagccatgtg gaggtgactg acacgctgat tgatgacaac aatgctatca tcaagaccag
     1081 cctgtgcgtg gaggtccggt atcaggccac tgacggcaca gtgggctcct gggacgatgg
     1141 ggacttcctg ggagatgagt ctcttcaaga ggaagagaag gacagccaag agacggatgg
     1201 actgctccca ggctcccggc ccagctcccg gcccccagga gagaagagct tccggagagc
     1261 cgggaggagc gtgttctccg ccatgaagct cggcaaaaac cggtctcaca aggaggagcc
     1321 ccaaagacca gatgaaccgg cggtgctgga gatggaagac cttgaccatc tggccattcg
     1381 gctaggagat ggactggatc ccgactcggt gtctctagcc tcagtcacag ctctcaccac
     1441 taatgtctcc aacaagcgat ctaagccaga cattaagatg gagccaagtg ctgggcggcc
     1501 catggattac caggtcagca tcacggtgat cgaggcccgg cagctggtgg gcttgaacat
     1561 ggaccctgtg gtgtgcgtgg aggtgggtga cgacaagaag tacacatcca tgaaggagtc
     1621 cactaactgc ccctattaca acgagtactt cgtcttcgac ttccatgtct ctccggatgt
     1681 catgtttgac aagatcatca agatttcggt gattcactcc aagaacctgc tgcgcagtgg
     1741 caccctggtg ggctccttca aaatggacgt gggaaccgtg tactcgcagc cagagcacca
     1801 gttccatcac aagtgggcca tcctgtctga ccccgatgac atctcctcgg ggctgaaggg
     1861 ctacgtgaag tgtgacgttg ccgtggtggg caaaggggac aacatcaaga cgccccacaa
     1921 ggccaatgag accgacgaag atgacattga ggggaacttg ctgctccccg agggggtgcc
     1981 ccccgaacgc cagtgggccc ggttctatgt gaaaatttac cgagcagagg ggctgccccg
     2041 tatgaacaca agcctcatgg ccaatgtaaa gaaggctttc atcggtgaaa acaaggacct
     2101 cgtggacccc tacgtgcaag tcttctttgc tggccagaag ggcaagactt cagtgcagaa
     2161 gagcagctat gagcccctgt ggaatgagca ggtcgtcttt acagacctct tccccccact
     2221 ctgcaaacgc atgaaggtgc agatccgaga ctcggacaag gtcaacgacg tggccatcgg
     2281 cacccacttc attgacctgc gcaagatttc taatgacgga gacaaaggct tcctgcccac
     2341 actgggccca gcctgggtga acatgtacgg ctccacacgt aactacacgc tgctggatga
     2401 gcatcaggac ctgaacgagg gcctggggga gggtgtgtcc ttccgggccc ggctcctgct
     2461 gggcctggct gtggagatcg tagacacctc caaccctgag ctcaccagct ccacagaggt
     2521 gcaggtggag caggccacgc ccatctcgga gagctgtgca ggtaaaatgg aagaattctt
     2581 tctctttgga gccttcctgg aggcctcaat gatcgaccgg agaaacggag acaagcccat
     2641 cacctttgag gtcaccatag gcaactatgg gaacgaagtt gatggcctgt cccggcccca
     2701 gcggcctcgg ccccggaagg agccggggga tgaggaagaa gtagacctga ttcagaacgc
     2761 aagtgatgac gaggccggtg atgccgggga cctggcctca gtctcctcca ctccaccaat
     2821 gcggccccag gtcaccgaca ggaactactt ccatctgccc tacctggagc gaaagccctg
     2881 catctacatc aagagctggt ggccggacca gcgccgccgc ctctacaatg ccaacatcat
     2941 ggaccacatt gccgacaagc tggaagaagg cctgaacgac atacaggaga tgatcaaaac
     3001 ggagaagtcc taccctgagc gtcgcctgcg gggcgtcctg gaggagctga gctgtggctg
     3061 ctgccgcttc ctctccctcg ctgacaagga ccagggccac tcatcccgca ccaggcttga
     3121 ccgggagcgc ctcaagtcct gcatgaggga gctggaaaac atggggcagc aggccaggat
     3181 gctgcgggcc caggtgaagc ggcacacggt gcgggacaag ctgaggctgt gccagaactt
     3241 cctgcagaag ctgcgcttcc tggcggacga gccccagcac agcattcccg acatcttcat
     3301 ctggatgatg agcaacaaca agcgtgtggc ctatgcccgt gtgccctcca aggacctgct
     3361 cttctccatc gtggaggagg agactggcaa ggactgcgcc aaggtcaaga cgctcttcct
     3421 taagctgcca gggaagcggg gcttcggttc ggcaggctgg acagtgcagg ccaaggtgga
     3481 gctctacctg tggctgggcc tcagcaaaca gcgcaaggag ttcctgtgcg gcctgccctg
     3541 tggcttccag gaggtcaagg cagcccaggg cctgggcctg catgccttcc cacccgtcag
     3601 cctggtctac accaagaagc aggcgttcca gctccgagcg cacatgtacc aggcccgcag
     3661 cctctttgcc gccgacagca gcggactctc agaccccttt gcccgcgtct tcttcatcaa
     3721 tcagagtcag tgcacagagg tgctgaatga gaccctgtgt cccacctggg accagatgct
     3781 ggtgttcgac aacctggagc tctatggtga agctcatgag ctgagggacg atccgcccat
     3841 cattgtcatt gaaatctatg accaggattc catgggcaaa gctgacttca tgggccggac
     3901 cttcgccaaa cccctggtga agatggcaga cgaggtgtac tgcccacccc gcttcccacc
     3961 tcagctcgag tactaccaga tctaccgtgg caacgccaca gctggagacc tgctggcggc
     4021 cttcgagctg ctgcagattg gaccagcagg gaaggctgac ctgcccccca tcaatggccc
     4081 ggtggacgtg gaccgaggtc ccatcatgcc cgtgcccatg ggcatccggc ccgtgctcag
     4141 caagtaccga gtggaggtgc tgttctgggg cctacgggac ctaaagcggg tgaacctggc
     4201 ccaggtggac cggccacggg tggacatcga gtgtgcaggg aaggtaagta tcaaggttac
     4261 aagacaggtt taaggagacc aataagctat tattcgtata ggatacttta tacgaagtta
     4321 ttaagttatg gagaccggtg tggcacgtgc ggaccgagcg gccgcaggaa cccctagtga
     4381 tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg
     4441 tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc
     4501 tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     4561 tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     4621 ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt
     4681 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     4741 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg
     4801 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     4861 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca actctatctc
     4921 gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt taaaaaatga
     4981 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg
     5041 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     5101 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     5161 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     5221 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     5281 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     5341 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     5401 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     5461 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     5521 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     5581 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     5641 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     5701 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     5761 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     5821 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     5881 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     5941 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     6001 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     6061 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     6121 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     6181 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     6241 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     6301 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     6361 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     6421 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     6481 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     6541 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     6601 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     6661 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     6721 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     6781 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     6841 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     6901 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     6961 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     7021 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     7081 ttgctggcct tttgctcaca tgt
//