| 质粒名称: | pbabe-c-mycT58A+HRasG12V |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 11130 |
| 存储实验室: | Christopher Counter |
|
载体骨架基本信息 |
|
| 载体名称: | pbabe |
| 载体出品公司: | Clontech |
| 空载体大小: | 6427 bp |
| 完整质粒大小: | 6427 bp |
| 载体类型: | 哺乳细胞表达, Retroviral |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | c-myc, HRas、c-myc、HRas |
| 物种来源: | H. sapiens (human), M. musculus (mouse) |
| 插入大小: | 2300 bp |
| 基因突变: | c-myc T58A, A156T, and G438R; and H-Ras G12V |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | pBABE 5' |
| 5’测序引物 | BamHI(c-myc), HindIII(H-Ras) (not destroyed) |
| 3’测序引物 | EcoRI(c-myc), ClaI(H-Ras) (not destroyed) |
|
文献引用方法 |
|
| 材料和方法部分: | pbabe-c-mycT58A+HRasG12V was a gift from Christopher Counter (Addgene plasmid # 11130) |
| 参考文献部分: | A network of genetic events sufficient to convert normal human cells to a tumorigenic state. Kendall SD, Linardic CM, Adam SJ, Counter CM. Cancer Res. 2005 Nov 1. 65(21):9824-8. 10.1158/0008-5472.CAN-05-1543 |
买家导航
pbabe-c-mycT58A+HRasG12V载体质粒基本信息
pbabe-c-mycT58A+HRasG12V质粒图谱载体图谱和pbabe-c-mycT58A+HRasG12V载体序列质粒序列多克隆位点信息
pbabe-c-mycT58A+HRasG12V质粒载体简介
pbabe-c-mycT58A+HRasG12V质粒序列载体序列
LOCUS pbabe-c-mycT58A+HRasG12V 6427 bp DNA circular SYN 15-DEC-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6427)
AUTHORS Kendall SD, Linardic CM, Adam SJ, Counter CM
TITLE A network of genetic events sufficient to convert normal human cells
to a tumorigenic state.
JOURNAL Cancer Res. 2005 Nov 1. 65(21):9824-8.
PUBMED 16267004
REFERENCE 2 (bases 1 to 6427)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Dec 15, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
2005 Nov 1. 65(21):9824-8."
COMMENT Sequence Label: pbabe-c-mycT58A+HRasG12V
FEATURES Location/Qualifiers
source 1..6427
/mol_type="other DNA"
/organism="synthetic DNA construct"
LTR 44..513
/note="long terminal repeat from Moloney murine leukemia
virus"
misc_feature 578..777
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
CDS 978..1394
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 1324..1346
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 1362..1378
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
primer_bind complement(2870..2890)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 2875..3204
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3055..3190
/label=SV40 ori
/note="SV40 origin of replication"
CDS join(3217..3249,3250..3252,3253..3786)
/codon_start=1
/product="human oncoprotein generated by the G12V mutation
in the small GTPase H-Ras"
/label=H-Ras (G12V)
/translation="MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVK
DSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREI
RQHKLRKLNPPDESGPGCMSCKCVLS"
LTR 3957..4426
/note="long terminal repeat from Moloney murine leukemia
virus"
primer_bind complement(4556..4573)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4727..5315)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4807..4826)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(5475..6266,6267..6335))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6098..6117
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(join(6336..6427,1..13))
/gene="bla"
/label=AmpR promoter
ORIGIN
1 taggggttcc gcgcacattt ccccgaaaag tgccacctgc agcctgaata tgggccaaac
61 aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggaacagct
121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
181 acagatggtc cccagatgcg gtccagccct cagcagtttc tagagaacca tcagatgttt
241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
361 ctcactcggg gcgccagtcc tccgattgac tgagtcgccc gggtacccgt gtatccaata
421 aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg
481 agtgattgac tacccgtcag cgggggtctt tcatttgggg gctcgtccgg gatcgggaga
541 cccctgccca gggaccaccg acccaccacc gggaggcaag ctggccagca acttatctgt
601 gtctgtccga ttgtctagtg tctatgactg attttatgcg cctgcgtcgg tactagttag
661 ctaactagct ctgtatctgg cggacccgtg gtggaactga cgagttctga acacccggcc
721 gcaaccctgg gagacgtccc agggactttg ggggccgttt ttgtggcccg acctgaggaa
781 gggagtcgat gtggaatccg accccgtcag gatatgtggt tctggtagga gacgagaacc
841 taaaacagtt cccgcctccg tctgaatttt tgctttcggt ttggaaccga agccgcgcgt
901 cttgtctgct gcagcgctgc agcatcgttc tgtgttgtct ctgtctgact gtgtttctgt
961 atttgtctga aaattagggc cagactgtta ccactccctt aagtttgacc ttaggtcact
1021 ggaaagatgt cgagcggatc gctcacaacc agtcggtaga tgtcaagaag agacgttggg
1081 ttaccttctg ctctgcagaa tggccaacct ttaacgtcgg atggccgcga gacggcacct
1141 ttaaccgaga cctcatcacc caggttaaga tcaaggtctt ttcacctggc ccgcatggac
1201 acccagacca ggtcccctac atcgtgacct gggaagcctt ggcttttgac ccccctccct
1261 gggtcaagcc ctttgtacac cctaagcctc cgcctcctct tcctccatcc gccccgtctc
1321 tcccccttga acctcctcgt tcgaccccgc ctcgatcctc cctttatcca gccctcactc
1381 cttctctagg cgccggccgg atcccagtgt ggtggtacgc ttgcatgcct gcaggtcgag
1441 ctgtttgaag gctggatttc ctttgggcgt tggaaacccc gcagacagcc acgacgatgc
1501 ccctcaacgt gaacttcacc aacaggaact atgacctcga ctacgactcc gtacagccct
1561 atttcatctg cgacgaggaa gagaatttct atcaccagca acagcagagc gagctgcagc
1621 cgcccgcgcc cagtgaggat atctggaaga aattcgagct gcttcccgcc ccgcccctgt
1681 ccccgagccg ccgctccggg ctctgctctc catcctatgt tgcggtcgct acgtccttct
1741 ccccaaggga agacgatgac ggcggcggtg gcaacttctc caccgccgat cagctggaga
1801 tgatgaccga gttacttgga ggagacatgg tgaaccagag cttcatctgc gatcctgacg
1861 acgagacctt catcaagaac atcatcatcc aggactgtat gtggagcggt ttctcagccg
1921 ctgccaagct ggtctcggag aagctggcct cctaccaggc tacgcgcaaa gacagcacca
1981 gcctgagccc cgcccgcggg cacagcgtct gctccacctc cagcctgtac ctgcaggacc
2041 tcaccgccgc cgcgtccgag tgcattgacc cctcagtggt ctttccctac ccgctcaacg
2101 acagcagctc gcccaaatcc tgtacctcgt ccgattccac ggccttctct ccttcctcgg
2161 actcgctgct gtcctccgag tcctccccac gggccagccc tgagccccta gtgctgcatg
2221 aggagacacc gcccaccacc agcagcgact ctgaagaaga gcaagaagat gaggaagaaa
2281 ttgatgtggt gtctgtggag aagaggcaaa cccctgccaa gaggtcggag tcgggctcat
2341 ctccatcccg aggccacagc aaacctccgc acagcccact ggtcctcaag aggtgccacg
2401 tctccactca ccagcacaac tacgccgcac ccccctccac aaggaaggac tatccagctg
2461 ccaagagggc caagttggac agtggcaggg tcctgaagca gatcagcaac aaccgcaagt
2521 gctccagccc caggtcctca gacacggagg aaaacgacaa gaggcggaca cacaacgtct
2581 tggaacgtca gaggaggaac gagctgaagc gcagcttttt tgccctgcgt gaccagatcc
2641 ctgaattgga aaacaacgaa aaggccccca aggtagtgat cctcaaaaaa gccaccgcct
2701 acatcctgtc cattcaagca gacgagcaca agctcacctc tgaaaaggac ttattgagga
2761 aacgacgaga acagttgaaa cacaaactcg aacagcttcg aaactctcgt gcataaactg
2821 acctaactcg actctagtag gaattcgcca gcacagtggt cgaccctgtg gaatgtgtgt
2881 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
2941 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
3001 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
3061 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
3121 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
3181 tttggaggcc taggcttttg caaaaagctt gccaccatga cggaatataa gctggtggtg
3241 gtgggcgccg tcggtgtggg caagagtgcg ctgaccatcc agctgatcca gaaccatttt
3301 gtggacgaat acgaccccac tatagaggat tcctaccgga agcaggtggt cattgatggg
3361 gagacgtgcc tgttggacat cctggatacc gccggccagg aggagtacag cgccatgcgg
3421 gaccagtaca tgcgcaccgg ggagggcttc ctgtgtgtgt ttgccatcaa caacaccaag
3481 tcttttgagg acatccacca gtacagggag cagatcaaac gggtgaagga ctcggatgac
3541 gtgcccatgg tgctggtggg gaacaagtgt gacctggctg cacgcactgt ggaatctcgg
3601 caggctcagg acctcgcccg aagctacggc atcccctaca tcgagacctc ggccaagacc
3661 cggcagggag tggaggatgc cttctacacg ttggtgcgtg agatccggca gcacaagctg
3721 cggaagctga accctcctga tgagagtggc cccggctgca tgagctgcaa gtgtgtgctc
3781 tcctgataaa tcgataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa
3841 agaccccacc tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa
3901 aaatacataa ctgagaatag agaagttcag atcaaggtca ggaacagatg gaacagctga
3961 atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac
4021 agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg
4081 ctcagggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagagaa
4141 ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact
4201 aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa
4261 gagcccacaa cccctcactc ggggcgccag tcctccgatt gactgagtcg cccgggtacc
4321 cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg
4381 agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcacatg cagcatgtat
4441 caaaattaat ttggtttttt ttcttaagta tttacattaa atggccatag ttgcattaat
4501 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
4561 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
4621 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
4681 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
4741 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
4801 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
4861 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
4921 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
4981 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
5041 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
5101 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
5161 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
5221 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
5281 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
5341 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
5401 aaaggatctt cacctagatc cttttgcggc cgcaaatcaa tctaaagtat atatgagtaa
5461 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
5521 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
5581 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
5641 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
5701 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
5761 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
5821 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
5881 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
5941 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
6001 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
6061 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
6121 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
6181 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
6241 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
6301 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
6361 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
6421 aaacaaa
//
