pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ABCA10-FLAG

LOCUS      pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ABCA10-FLAG            7260 bp DNA     circular SYN 24-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7260)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 10, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7260
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..3973
                     /codon_start=1
                     /gene="ABCA10"
                     /gene_synonym="EST698739"
                     /product="ATP-binding cassette sub-family A member 10"
                     /label=ABCA10
                     /note="ATP-binding cassette A10; ATP-binding cassette, 
                     sub-family A (ABC1), member 10"
                     /db_xref="GI:1789691080"
                     /db_xref="CCDS:CCDS11684.1"
                     /db_xref="GeneID:10349"
                     /db_xref="HGNC:HGNC:30"
                     /db_xref="MIM:612508"
                     /protein_id="NP_001364250.1"
                     /translation="VLCSLPETRKAVSSAALWRRQIYAVATLRFLKLRRERRALLCLLL
                     VLGIAFIPIILEKIMYKVTRETHCWEFSPSMYFLSLEQIPKTPLTSLLIVNNTGSNIED
                     LVHSLKCQDIVLEIDDFRNRNGSDDPSYNGAIIVSGDQKDYRFSVACNTKKLNCFPVLM
                     GIVSNALMGIFNFTELIQMESTSFSRDDIVLDLGFIDGSIFLLLITNCVSPFIGMSSIS
                     DYKKNVQSQLWISGLWPSAYWCGQALVDIPLYFLILFSIHLIYYFIFLGFQLSWELMFV
                     LVVCIIGCAVSLIFLTYVLSFIFRKWRKNNGFWSFGFFIILICVSTIMVSTQYEKLNLI
                     LCMIFIPSFTLLGYVMLLIQLDFMRNLDSLDNRINEVNKTILLTTLIPYLQSVIFLFVI
                     RCLEMKYGNEIMNKDPVFRISPRSRETHPNPEEPEEEDEDVQAERVQAANALTAPNLEE
                     EPVITASCLHKEYYETKKSCFSTRKKKIAIRNVSFCVKKGEVLGLLGHNGAGKSTSIKM
                     ITGCTKPTAGVVVLQGSRASVRQQHDNSLKFLGYCPQENSLWPKLTMKEHLELYAAVKG
                     LGKEDAALSISRLVEALKLQEQLKAPVKTLSEGIKRKLCFVLSILGNPSVVLLDEPFTG
                     MDPEGQQQMWQILQATVKNKERGTLLTTHYMSEAEAVCDRMAMMVSGTLRCIGSIQHLK
                     NKFGRDYLLEIKMKEPTQVEALHTEILKLFPQAAWQERYSSLMAYKLPVEDVHPLSRAF
                     FKLEAMKQTFNLEEYSLSQATLEQVFLELCKEQELGNVDDKIDTTVEWKLLPQEDP"
     misc_feature    1935..1940
                     /label=SDS
     misc_feature    2916..2921
                     /label=SDS
     CDS             3980..4045
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      4049..4480
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    4193..4198
                     /label=SDS
     repeat_region   4523..4663
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4523..4652
                     /label=AAV2 ITR
     repeat_region   4524..4664
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4738..5193
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5475..5579
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5580..6440
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6611..7199
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggttc tttgttctct
     1561 tcctgaaaca agaaaggctg tcagtagtgc agctctctgg agacgacaaa tctatgcagt
     1621 ggcaacactt cgcttcttaa agttaaggcg tgaaaggaga gctcttttgt gtttgttact
     1681 agtacttgga attgctttta tccccatcat tctagagaag ataatgtata aagtaactcg
     1741 tgaaactcat tgttgggagt tttcacccag tatgtatttc ctttctctgg aacaaatccc
     1801 gaagacgcct cttaccagcc tgttaatcgt taataataca ggatcaaata ttgaagacct
     1861 cgtgcattca ctgaagtgtc aggatatagt tttggaaata gatgacttta gaaacagaaa
     1921 tggctcagat gatccctcct acaatggagc catcatagtg tctggtgacc agaaggatta
     1981 cagattttct gttgcgtgta ataccaagaa attgaattgt tttcctgttc ttatgggaat
     2041 tgttagcaat gcccttatgg gaatttttaa cttcacggag cttattcaaa tggagagcac
     2101 ttcattttct cgtgatgaca tagtgctgga tcttggtttt atagatgggt ccatattttt
     2161 gttgttgatc acaaactgcg tttctccttt tatcggcatg agcagcatca gcgattataa
     2221 aaaaaatgtt caatcccagt tatggatttc aggcctctgg ccttcagcat actggtgtgg
     2281 acaggctctg gtggacattc cattatactt cttgattctc ttttcaatac atttaattta
     2341 ctacttcata tttctgggat tccagctttc atgggaactc atgtttgttt tggtggtatg
     2401 cataattggt tgtgcagttt ctcttatatt cctcacatat gtgctttcat tcatctttcg
     2461 caagtggaga aaaaataatg gcttttggtc ttttggcttt tttattatct taatatgtgt
     2521 atccacaatt atggtatcaa ctcaatatga aaaactcaac ttaattttgt gcatgatttt
     2581 cataccttcc ttcactttgc tggggtatgt catgttattg atccagctcg actttatgag
     2641 aaacttggac agtctggaca atagaataaa tgaagtcaat aaaaccattc ttttaacaac
     2701 cttaatacca taccttcaga gtgttatttt cctttttgtc ataaggtgtc tggaaatgaa
     2761 gtatggaaat gaaataatga ataaagaccc agttttcaga atctctccac ggagtagaga
     2821 aactcatccc aatccggaag agcccgaaga agaagatgaa gatgttcaag ctgaaagagt
     2881 ccaagcagca aatgcactca ctgctccaaa cttggaggag gaaccagtca taactgcaag
     2941 ctgtttacac aaggaatatt atgagacaaa gaaaagttgc ttttcaacaa gaaagaagaa
     3001 aatagccatc agaaatgttt ccttttgtgt taaaaaaggt gaagttttgg gattactagg
     3061 acacaatgga gctggtaaaa gtacttccat taaaatgata actgggtgca caaagccaac
     3121 tgcaggagtg gtggtgttac aaggcagcag agcatcagta aggcaacagc atgacaacag
     3181 cctcaagttc ttggggtact gccctcagga gaactcactg tggcccaagc ttacaatgaa
     3241 agagcacttg gagttgtatg cagctgtgaa aggactgggc aaagaagatg ctgctctcag
     3301 tatttcacga ttggtggaag ctcttaagct ccaggaacaa cttaaggctc ctgtgaaaac
     3361 tctatcagag ggaataaaga gaaagctgtg ctttgtgctg agcatcctgg ggaacccatc
     3421 agtggtgctt ctagatgagc cgttcaccgg gatggacccc gaggggcagc agcaaatgtg
     3481 gcagatactt caggctaccg ttaaaaacaa ggagaggggc accctcttga ccacccatta
     3541 catgtcagag gctgaggctg tgtgtgaccg tatggccatg atggtgtcag gaacgctaag
     3601 gtgtattggt tccattcaac atctgaaaaa caagtttggt agagattatt tactagaaat
     3661 aaaaatgaaa gaacctaccc aggtggaagc tctccacaca gagattttga agcttttccc
     3721 acaggctgct tggcaggaaa gatattcctc tttaatggcg tataagttac ctgtggagga
     3781 tgtccaccct ctatctcggg cctttttcaa gttagaggcg atgaaacaga ccttcaacct
     3841 ggaggaatac agcctctctc aggctacctt ggagcaggta ttcttagaac tctgtaaaga
     3901 gcaggagctg ggaaatgttg atgataaaat tgatacaaca gttgaatgga aacttctccc
     3961 acaggaagac ccttgtacag actacaaaga ccatgacggt gattataaag atcatgacat
     4021 cgactacaag gatgacgatg acaagtaact cgagataatc aacctctgga ttacaaaatt
     4081 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     4141 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     4201 tataaatcct ggttagttct tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     4261 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgtttatttg tgaaatttgt
     4321 gatgctattg ctttatttgt aaccatctag ctttatttgt gaaatttgtg atgctattgc
     4381 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     4441 tatgtttcag gttcaggggg agatgtggga ggttttttaa agatctggag accggtgtgg
     4501 cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg
     4561 cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc
     4621 cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat
     4681 tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg
     4741 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     4801 cacttgccag cgccttagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     4861 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     4921 ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat
     4981 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     5041 tcttgttcca aactggaaca acactcaact ctatctcggg ctattctttt gatttataag
     5101 ggattttgcc gatttcggtc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     5161 cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct
     5221 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     5281 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     5341 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     5401 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     5461 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     5521 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     5581 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     5641 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     5701 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     5761 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     5821 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     5881 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     5941 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     6001 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     6061 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     6121 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     6181 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     6241 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     6301 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     6361 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     6421 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     6481 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     6541 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     6601 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     6661 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     6721 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     6781 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     6841 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     6901 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     6961 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     7021 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     7081 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     7141 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     7201 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
//