| 质粒名称: | AbVec2.1-mIglc2 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 127156 |
| 存储实验室: | Vladislav Verkhusha |
|
载体骨架基本信息 |
|
| 载体名称: | pUC19 |
| 载体出品公司: | Clontech |
| 空载体大小: | 5729bp |
| 完整质粒大小: | 5087 bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Iglc2 |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 315bp |
| GenBank编号 | LR588434 |
| 启动子: | CMV IE1 gene promoter |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | AgeI(未破坏) |
| 3’克隆位点: | MscI (not destroyed) |
| 5’测序引物 | GCTTCGTTAGAACGCGGCTAC |
|
文献引用方法 |
|
| 材料和方法部分: | AbVec2.1-mIglc2 was a gift from Hedda Wardemann (Addgene plasmid # 127156 ; http://n2t.net/addgene:127156 ; RRID:Addgene_127156) |
| 参考文献部分: |
ALDH4A1 is an atherosclerosis auto-antigen targeted by protective antibodies. Lorenzo C, Delgado P, Busse CE, Sanz-Bravo A, Martos-Folgado I, Bonzon-Kulichenko E, Ferrarini A, Gonzalez-Valdes IB, Mur SM, Roldan-Montero R, Martinez-Lopez D, Martin-Ventura JL, Vazquez J, Wardemann H, Ramiro AR. Nature. 2021 Jan;589(7841):287-292. doi: 10.1038/s41586-020-2993-2. Epub 2020 Dec 2. 10.1038/s41586-020-2993-2 PubMed 33268892
|
买家导航
AbVec2.1-mIglc2载体质粒基本信息
AbVec2.1-mIglc2质粒图谱载体图谱和AbVec2.1-mIglc2载体序列质粒序列多克隆位点信息
AbVec2.1-mIglc2质粒载体简介
AbVec2.1-mIglc2质粒序列载体序列
LOCUS AbVec2.1-mIglc2 5087 bp DNA circular SYN 25-AUG-2024
DEFINITION Expression of immunoglobulin light chains in mammalian cells, mouse
(C57BL/6), lambda 2 constant region.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5087)
AUTHORS Lorenzo C, Delgado P, Busse CE, Sanz-Bravo A, Martos-Folgado I,
Bonzon-Kulichenko E, Ferrarini A, Gonzalez-Valdes IB, Mur SM,
Roldan-Montero R, Martinez-Lopez D, Martin-Ventura JL, Vazquez J,
Wardemann H, Ramiro AR
TITLE ALDH4A1 is an atherosclerosis auto-antigen targeted by protective
antibodies.
JOURNAL Nature. 2021 Jan;589(7841):287-292. doi: 10.1038/s41586-020-2993-2.
Epub 2020 Dec 2.
PUBMED 33268892
REFERENCE 2 (bases 1 to 5087)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 25, 2024 from SnapGene Server 7.0.3
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1038/s41586-020-2993-2"; journalName: "Nature"; date: "2021-01";
volume: "589"; issue: "7841"; pages: "287-292"
FEATURES Location/Qualifiers
source 1..5087
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind 106..127
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 142..172
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 180..196
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 185..207
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 204..220
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 204..220
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
enhancer 249..628
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 629..832
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 783..803
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 829..853
/label=LNCX
/note="Human CMV promoter, forward primer"
promoter 1063..1081
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind 1063..1080
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind 1094..1113
/label=pMT2-F
/note="Synthetic intron, forward primer"
regulatory 1622..1631
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
polyA_signal 1635..1769
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1672..1691)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 1726..1745
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
promoter 1838..2195
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
enhancer 1838..2029
/label=SV40 enhancer
/note="enhancer for the SV40 early promoter (Herr, 1993)"
primer_bind complement(1861..1881)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 2046..2181
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind complement(2215..2232)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(2215..2231)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2224..2246)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 2444..2899
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2531..2550)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 2741..2762
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(2916..2935)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 3035..3057
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(3095..3113)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 3181..3285
/gene="bla"
/label=AmpR promoter
CDS 3286..4146
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(3504..3523)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 4317..4905
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4806..4825
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 5059..5076
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat ccaactggca
61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
181 tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga attaattcga
241 gctcgcccga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
301 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
361 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
421 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
481 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
541 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
601 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
661 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
721 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
781 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt
841 gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg
901 ggaccgatcc agcctccgcg gccgggaacg gtgcattgga acgcggattc cccgtgccaa
961 gagtgacgta agtaccgcct atagagtcta taggcccacc cccttggctt cgttagaacg
1021 cggctacaat taatacataa ccttatgtat catacacata cgatttaggt gacactatag
1081 aataacatcc actttgcctt tctctccaca ggtgtccact cccaggtcca actgcacctc
1141 ggttctatcg attgaattcc accatgggat ggtcatgtat catccttttt ctagtagcaa
1201 ctgcaaccgg ttgactaact agctggccag cccaagagca ctcccactct caccgtgttt
1261 ccaccttcct ctgaggagct caaggaaaac aaagccacac tggtgtgtct gatttccaac
1321 ttttccccga gtggtgtgac agtggcctgg aaggcaaatg gtacacctat cacccagggt
1381 gtggacactt caaatcccac caaagagggc aacaagttca tggcctccag cttcctacat
1441 ttgacatcgg accagtggag atctcacaac agttttacct gtcaagttac acatgaaggg
1501 gacactgtgg agaagagtct gtctcctgca gaatgtctct aagaacccag gtttctcctt
1561 agcctgggaa ccctgcagct tttagagacc cagggtgggg tctcttcttt atataagctt
1621 ggccgccatg gcccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
1681 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
1741 tcatcaatgt atcttatcat gtctggatcg ggaattaatt cggcgcagca ccatggcctg
1801 aaataacctc tgaaagagga acttggttag gtaccttctg aggcggaaag aaccatctgt
1861 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
1921 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
1981 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
2041 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
2101 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
2161 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct gttaacagct tggcactggc
2221 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
2281 agcacatccc cctttcgcca gttggcgtaa tagcgaagag gcccgcaccg atcgcccttc
2341 ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca
2401 tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc
2461 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
2521 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
2581 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
2641 gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
2701 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
2761 ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt
2821 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
2881 atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag
2941 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
3001 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
3061 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
3121 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
3181 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
3241 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
3301 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
3361 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
3421 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
3481 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
3541 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
3601 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
3661 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
3721 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
3781 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
3841 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
3901 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
3961 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
4021 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
4081 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
4141 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
4201 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
4261 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
4321 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
4381 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
4441 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
4501 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
4561 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
4621 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
4681 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
4741 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
4801 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
4861 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
4921 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
4981 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
5041 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaaga
//
