| 质粒名称: | pBait-YY1 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 165147 |
| 存储实验室: | Vladislav Verkhusha |
|
载体骨架基本信息 |
|
| 载体名称: | pBait |
| 载体出品公司: | Clontech |
| 完整质粒大小: | 4989 bp |
| 载体类型: | Unspecified |
| 载体抗性: | Kanamycin, 50 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | 3X YY1 motif |
| 插入大小: | 4818bp |
|
文献引用方法 |
|
| 材料和方法部分: | pBait-YY1 was a gift from Paul Khavari (Addgene plasmid # 165147 ; http://n2t.net/addgene:165147 ; RRID:Addgene_165147) |
| 参考文献部分: | PROBER identifies proteins associated with programmable sequence-specific DNA in living cells. Mondal S, Ramanathan M, Miao W, Meyers RM, Rao D, Lopez-Pajares V, Siprashvili Z, Reynolds DL, Porter DF, Ferguson I, Neela P, Zhao Y, Meservey LM, Guo M, Yang YY, Li L, Wang Y, Khavari PA. Nat Methods. 2022 Aug;19(8):959-968. doi: 10.1038/s41592-022-01552-w. Epub 2022 Aug 4. 10.1038/s41592-022-01552-w PubMed 35927480 |
买家导航
pBait-YY1载体质粒基本信息
pBait-YY1质粒图谱载体图谱和pBait-YY1载体序列质粒序列多克隆位点信息
pBait-YY1质粒载体简介
pBait-YY1质粒序列载体序列
LOCUS pBait-YY1 4818 bp DNA circular SYN 14-APR-2025
DEFINITION Bait plasmid for PROBER with YY1-binding site (positive control).
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4818)
AUTHORS Mondal S, Ramanathan M, Miao W, Meyers RM, Rao D, Lopez-Pajares V,
Siprashvili Z, Reynolds DL, Porter DF, Ferguson I, Neela P, Zhao Y,
Meservey LM, Guo M, Yang YY, Li L, Wang Y, Khavari PA
TITLE PROBER identifies proteins associated with programmable
sequence-specific DNA in living cells.
JOURNAL Nat Methods. 2022 Aug;19(8):959-968. doi:
10.1038/s41592-022-01552-w. Epub 2022 Aug 4.
PUBMED 35927480
REFERENCE 2 (bases 1 to 4818)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1038/s41592-022-01552-w"; journalName: "Nat Methods"; date:
"2022-08"; volume: "19"; issue: "8"; pages: "959-968"
COMMENT Sequence Label: pBait-YY1
FEATURES Location/Qualifiers
source 1..4818
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 1..239
/label=hsp70 promoter
/note="Drosophila melanogaster hsp70Bb promoter"
primer_bind 186..207
/label=pCasper-hs
/note="Drosophila Hsp70 promoter, forward primer"
primer_bind complement(1045..1062)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 1051..1162
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(1181..1201)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 1186..1515
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
CDS 1575..2174
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(1575..1594)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 2071..2091
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
polyA_signal 2177..2298
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2214..2233)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 2268..2287
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(2445..2462)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(2555..3143)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2635..2654)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(3317..4111)
/codon_start=1
/product="aminoglycoside phosphotransferase"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
promoter complement(4112..4216)
/gene="bla"
/label=AmpR promoter
primer_bind 4517..4539
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 4531..4548
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 4532..4548
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 4559..4578
/label=T7
/note="T7 promoter, forward primer"
promoter 4559..4577
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 4582..4593
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
ORIGIN
1 ccggagtata aatagaggcg cttcgtctac ggagcgacaa ttcaattcaa acaagcaaag
61 tgaacacgtc gctaagcgaa agctaagcaa ataaacaagc gcagctgaac aagctaaaca
121 atctgcagta aagtgcaagt taaagtgaat caattaaaag taaccagcaa ccaagtaaat
181 caactgcaac tactgaaatc tgccaagaag taattattga atacaagaag agaactctga
241 atagggaatt gggaattcgt taacagatcc gatatccgcc accaagcttc tgtctagagc
301 cgccaccatg actgccctga ccgaaggtgc taagctgttt gagaaggaga ttccgtacat
361 caccgagctg gaaggggacg tcgaaggaat gaagttcatc atcaagggag aaggaaccgg
421 ggacgctacg actggaacca ttaaggccaa gtatatctgt accactggag atctgccagt
481 gccttgggcc acccttgtgt caaccctctc gtatggagtg cagtgttttg ctaagtaccc
541 tagccacatt aaggacttct tcaaatccgc catgccggaa ggttataccc aagagcgcac
601 catttctttt gagggagatg gagtgtacaa gacccgcgcg atggtcacct atgagagggg
661 atctatctac aaccgggtga ctctgactgg agaaaacttt aagaaggacg ggcatattct
721 tcggaagaat gtcgccttcc agtgccctcc cagcatcctt tacattctcc ccgacactgt
781 gaacaacgga atccgcgtgg agttcaatca agcctacgac atcgaggggg tgacggagaa
841 gctggtgacc aagtgtagcc agatgaatcg gccactggcc ggttcagcgg ctgtccacat
901 tccgcgctac catcatatca cttatcacac taagctctcc aaagaccgcg atgagaggag
961 agatcacatg tgcctggtgg aagtggtcaa ggccgtcgat ctcgatacct atcagtaaac
1021 ctgctctcaa aactgtcttc tcagcctcga ctgtgccttc tagttgccag ccatctgttg
1081 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
1141 aataaaatga ggaaattgca tcgaagacag tcagccttgt ggaatgtgtg tcagttaggg
1201 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
1261 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
1321 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
1381 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
1441 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggt
1501 ataggctttt gcaaaaagct ctctggctaa ctagagaacc cactgcttac tggcttatcg
1561 tttaagccgc caccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
1621 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
1681 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
1741 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
1801 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
1861 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
1921 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
1981 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
2041 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
2101 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
2161 agcccggtgc ctgaccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
2221 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
2281 actcatcaat gtatcttatc atgtcggatg agcggtatca gctcactcaa aggcccctcg
2341 agggccttcc tttcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
2401 catggtcata gctgtttcct tgcgtattgg gcgctctccg cttcctcgct cactgactcg
2461 ctgcgctcgg tcgttcgggt aaagcctggg gtgcctaatg agcaaaaggc cagcaaaagg
2521 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
2581 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
2641 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
2701 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
2761 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
2821 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
2881 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
2941 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
3001 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
3061 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
3121 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
3181 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
3241 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
3301 cttggtctga cagttattag aaaaattcat ccagcagacg ataaaacgca atacgctggc
3361 tatccggtgc cgcaatgcca tacagcacca gaaaacgatc cgcccattcg ccgcccagtt
3421 cttccgcaat atcacgggtg gccagcgcaa tatcctgata acgatccgcc acgcccagac
3481 ggccgcaatc aataaagccg ctaaaacggc cattttccac cataatgttc ggcaggcacg
3541 catcaccatg ggtcaccacc agatcttcgc catccggcat gctcgctttc agacgcgcaa
3601 acagctctgc cggtgccagg ccctgatgtt cttcatccag atcatcctga tccaccaggc
3661 ccgcttccat acgggtacgc gcacgttcaa tacgatgttt cgcctgatga tcaaacggac
3721 aggtcgccgg gtccagggta tgcagacgac gcatggcatc cgccataatg ctcacttttt
3781 ctgccggcgc cagatggcta gacagcagat cctgacccgg cacttcgccc agcagcagcc
3841 aatcacggcc cgcttcggtc accacatcca gcaccgccgc acacggaaca ccggtggtgg
3901 ccagccagct cagacgcgcc gcttcatcct gcagctcgtt cagcgcaccg ctcagatcgg
3961 ttttcacaaa cagcaccgga cgaccctgcg cgctcagacg aaacaccgcc gcatcagagc
4021 agccaatggt ctgctgcgcc caatcatagc caaacagacg ttccacccac gctgccgggc
4081 tacccgcatg caggccatcc tgttcaatca tactcttcct ttttcaatat tattgaagca
4141 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
4201 aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa gcgttaatat
4261 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
4321 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtggccgcta
4381 cagggcgctc ccattcgcca ttcaggctgc gcaactgttg ggaagggcgt ttcggtgcgg
4441 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
4501 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgacgta
4561 atacgactca ctatagggcg aattgaagga aggccgtcaa ggccgcatac actgcggggg
4621 ctctggagac gcgtcggagg acagtactcc gagcggagga cagtactccg agcggaggac
4681 agtactccga ggccgccatc ttggacgccg ccatcttgga cgccgccatc ttggacggag
4741 gacagtactc cgagcggagg acagtactcc gagcggagga cagtactccg agcctctaga
4801 cggagactct agcgagcg
//
