| 质粒名称: | pmGFP-ADAR1-p110 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 117928 |
| 存储实验室: | Kumiko Ui-Tei |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 载体出品公司: | Invitrogen |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 5428 bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | ADAR1-p110 |
| 物种来源: | H. sapiens (human) |
| GenBank编号 | NM_001193495 NM_001193495 |
| 启动子 | CMV |
| 标签/融合蛋白: | myc GFP（插入件上的N端子） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | HindIII(未破坏) |
| 3' 克隆位点: | XbaI (未破坏) |
| 5' 测序引物 | AAAGGGaagcttATGGCCGAGATCAAGGAGAAAATC |
| 3' 测序引物 | AAAGGGtctagaCTATACTGGGCAGAGATAAAAGTTC |
|
文献引用方法 |
|
| 材料和方法部分: | pmGFP-ADAR1-p110 was a gift from Kumiko Ui-Tei (Addgene plasmid # 117928 ; http://n2t.net/addgene:117928 ; RRID:Addgene_117928) |
| 参考文献部分: | Differential Binding of Three Major Human ADAR Isoforms to Coding and Long Non-Coding Transcripts. Galipon J, Ishii R, Suzuki Y, Tomita M, Ui-Tei K. Genes (Basel). 2017 Feb 11;8(2). pii: genes8020068. doi: 10.3390/genes8020068. 10.3390/genes8020068 PubMed 28208661 |
买家导航
pmGFP-ADAR1-p110载体质粒基本信息
pmGFP-ADAR1-p110质粒图谱载体图谱和pmGFP-ADAR1-p110载体序列质粒序列多克隆位点信息
pmGFP-ADAR1-p110质粒载体简介
pmGFP-ADAR1-p110质粒序列载体序列
LOCUS pmGFP-ADAR1-p110 8916 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION ADAR1-p110 expression plasmid..
ACCESSION .
VERSION .
KEYWORDS pmGFP-ADAR1-p110
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8916)
AUTHORS Galipon J, Ishii R, Suzuki Y, Tomita M, Ui-Tei K
TITLE Differential Binding of Three Major Human ADAR Isoforms to Coding
and Long Non-Coding Transcripts.
JOURNAL Genes (Basel). 2017 Feb 11;8(2). pii: genes8020068. doi:
10.3390/genes8020068.
PUBMED 28208661
REFERENCE 2 (bases 1 to 8916)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8916
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(146..165)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 493..514
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind complement(3399..3416)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 3405..3629
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 3675..4103
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3762..3781)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 3972..3993
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(4112..4132)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 4117..4446
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 4297..4432
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 4359..4378
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 4513..5307
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(4567..4586)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 5177..5196
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 5481..5602
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5518..5537)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5572..5591
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(5651..5667)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5651..5667)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5664..5686)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5675..5691
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5699..5729)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5744..5765
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(5882..5899)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6053..6641)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6133..6152)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6812..7672)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7435..7454
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7673..7777)
/gene="bla"
/label=AmpR promoter
primer_bind complement(7852..7871)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 8043..8422
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 8423..8626
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 8577..8597
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 8671..8690
/label=T7
/note="T7 promoter, forward primer"
promoter 8671..8689
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 8717..8746
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 8756..556
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 8756..556
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(8801..8822)
/label=EGFP-N
/note="EGFP, reverse primer"
ORIGIN
1 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
61 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
121 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
181 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
241 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
301 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
361 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
421 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
481 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
541 ggacgagctg tacaagtccg gcggcaagct tatggccgag atcaaggaga aaatctgcga
601 ctatctcttc aatgtgtctg actcctctgc cctgaatttg gctaaaaata ttggccttac
661 caaggcccga gatataaatg ctgtgctaat tgacatggaa aggcaggggg atgtctatag
721 acaagggaca acccctccca tatggcattt gacagacaag aagcgagaga ggatgcaaat
781 caagagaaat acgaacagtg ttcctgaaac cgctccagct gcaatccctg agaccaaaag
841 aaacgcagag ttcctcacct gtaatatacc cacatcaaat gcctcaaata acatggtaac
901 cacagaaaaa gtggagaatg ggcaggaacc tgtcataaag ttagaaaaca ggcaagaggc
961 cagaccagaa ccagcaagac tgaaaccacc tgttcattac aatggcccct caaaagcagg
1021 gtatgttgac tttgaaaatg gccagtgggc cacagatgac atcccagatg acttgaatag
1081 tatccgcgca gcaccaggtg agtttcgagc catcatggag atgccctcct tctacagtca
1141 tggcttgcca cggtgttcac cctacaagaa actgacagag tgccagctga agaaccccat
1201 cagcgggctg ttagaatatg cccagttcgc tagtcaaacc tgtgagttca acatgataga
1261 gcagagtgga ccaccccatg aacctcgatt taaattccag gttgtcatca atggccgaga
1321 gtttccccca gctgaagctg gaagcaagaa agtggccaag caggatgcag ctatgaaagc
1381 catgacaatt ctgctagagg aagccaaagc caaggacagt ggaaaatcag aagaatcatc
1441 ccactattcc acagagaaag aatcagagaa gactgcagag tcccagaccc ccaccccttc
1501 agccacatcc ttcttttctg ggaagagccc cgtcaccaca ctgcttgagt gtatgcacaa
1561 attggggaac tcctgcgaat tccgtctcct gtccaaagaa ggccctgccc atgaacccaa
1621 gttccaatac tgtgttgcag tgggagccca aactttcccc agtgtgagtg ctcccagcaa
1681 gaaagtggca aagcagatgg ccgcagagga agccatgaag gccctgcatg gggaggcgac
1741 caactccatg gcttctgata accagcctga aggtatgatc tcagagtcac ttgataactt
1801 ggaatccatg atgcccaaca aggtcaggaa gattggcgag ctcgtgagat acctgaacac
1861 caaccctgtg ggtggccttt tggagtacgc ccgctcccat ggctttgctg ctgaattcaa
1921 gttggtcgac cagtccggac ctcctcacga gcccaagttc gtttaccaag caaaagttgg
1981 gggtcgctgg ttcccagccg tctgcgcaca cagcaagaag caaggcaagc aggaagcagc
2041 agatgcggct ctccgtgtct tgattgggga gaacgagaag gcagaacgca tgggtttcac
2101 agaggtaacc ccagtgacag gggccagtct cagaagaact atgctcctcc tctcaaggtc
2161 cccagaagca cagccaaaga cactccctct cactggcagc accttccatg accagatagc
2221 catgctgagc caccggtgct tcaacactct gactaacagc ttccagccct ccttgctcgg
2281 ccgcaagatt ctggccgcca tcattatgaa aaaagactct gaggacatgg gtgtcgtcgt
2341 cagcttggga acagggaatc gctgtgtaaa aggagattct ctcagcctaa aaggagaaac
2401 tgtcaatgac tgccatgcag aaataatctc ccggagaggt ttcatcaggt ttctctacag
2461 tgagttaatg aaatacaact cccagactgc gaaggatagt atatttgaac ctgctaaggg
2521 aggagaaaag ctccaaataa aaaagactgt gtcattccat ctgtatatca gcactgctcc
2581 gtgtggagat ggcgccctct ttgacaagtc ctgcagcgac cgtgctatgg aaagcacaga
2641 atcccgccac taccctgtct tcgagaatcc caaacaagga aagctccgca ccaaggtgga
2701 gaacggagaa ggcacaatcc ctgtggaatc cagtgacatt gtgcctacgt gggatggcat
2761 tcggctcggg gagagactcc gtaccatgtc ctgtagtgac aaaatcctac gctggaacgt
2821 gctgggcctg caaggggcac tgttgaccca cttcctgcag cccatttatc tcaaatctgt
2881 cacattgggt taccttttca gccaagggca tctgacccgt gctatttgct gtcgtgtgac
2941 aagagatggg agtgcatttg aggatggact acgacatccc tttattgtca accaccccaa
3001 ggttggcaga gtcagcatat atgattccaa aaggcaatcc gggaagacta aggagacaag
3061 cgtcaactgg tgtctggctg atggctatga cctggagatc ctggacggta ccagaggcac
3121 tgtggatggg ccacggaatg aattgtcccg ggtctccaaa aagaacattt ttcttctatt
3181 taagaagctc tgctccttcc gttaccgcag ggatctactg agactctcct atggtgaggc
3241 caagaaagct gcccgtgact acgagacggc caagaactac ttcaaaaaag gcctgaagga
3301 tatgggctat gggaactgga ttagcaaacc ccaggaggaa aagaactttt atctctgccc
3361 agtatagtct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
3421 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
3481 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
3541 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
3601 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
3661 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
3721 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
3781 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
3841 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
3901 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
3961 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
4021 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
4081 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
4141 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
4201 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
4261 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
4321 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
4381 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
4441 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga
4501 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
4561 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
4621 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
4681 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
4741 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
4801 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
4861 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
4921 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
4981 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
5041 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
5101 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
5161 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
5221 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
5281 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
5341 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
5401 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
5461 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
5521 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
5581 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
5641 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
5701 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
5761 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
5821 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
5881 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
5941 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
6001 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
6061 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
6121 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
6181 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
6241 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
6301 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
6361 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
6421 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
6481 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
6541 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
6601 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
6661 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
6721 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
6781 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
6841 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
6901 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
6961 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
7021 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
7081 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
7141 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
7201 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
7261 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
7321 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
7381 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
7441 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
7501 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
7561 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
7621 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
7681 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
7741 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
7801 ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg
7861 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga
7921 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa
7981 gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg
8041 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
8101 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
8161 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
8221 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
8281 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
8341 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
8401 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
8461 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
8521 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
8581 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag
8641 agaacccact gcttactggc ttatcgaaat taatacgact cactataggg agacccaagc
8701 tggctagccc accatggaac aaaaactcat ctcagaagag gatctgtccg gcggcatggt
8761 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
8821 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
8881 gctgaccctg aagttcatct gcaccaccgg caagct
//
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