| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-MINK1-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001888 |
| 产品用途: | 3' AAVLINK plasmid for MINK1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | MINK1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-MINK1-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-MINK1-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-MINK1-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-MINK1-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-MINK1-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-MINK1-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-MINK1-FLAG 6671 bp DNA circular SYN 25-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6671)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 12, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6671
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
/note="color: #ffe4c4"
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3384
/codon_start=1
/gene="MINK1"
/gene_synonym="B55; MAP4K6; MEKKK 6; MINK; YSK2; ZC3"
/product="misshapen-like kinase 1 isoform 3"
/EC_number="2.7.11.1"
/label=MINK1
/note="isoform 3 is encoded by transcript variant 3;
misshapen/NIK-related kinase; GCK family kinase MINK; MEK
kinase kinase 6; MAPK/ERK kinase kinase kinase 6;
mitogen-activated protein kinase kinase kinase kinase 6"
/db_xref="CCDS:CCDS45588.1"
/db_xref="GeneID:50488"
/db_xref="HGNC:HGNC:17565"
/db_xref="MIM:609426"
/protein_id="NP_722549.2"
/translation="PPAQPPGPPNASSNPDLRRSDPGWERSDSVLPASHGHLPQAGSLE
RNRVGASSKLDSSPVLSPGNKAKPDDHRSRPGRPADFVLLKERTLDEAPRPPKKAMDYS
SSSEEVESSEDDEEEGEGGPAEGSRDTPGGRSDGDTDSVSTMVVHDVEEITGTQPPYGG
GTMVVQRTPEEERNLLHADSNGYTNLPDVVQPSHSPTENSKGQSPPSKDGSGDYQSRGL
VKAPGKSSFTMFVDLGIYQPGGSGDSIPITALVGGEGTRLDQLQYDVRKGSVVNVNPTN
TRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRF
QQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHY
RVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLK
VIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYV
NTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRL
KFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW"
misc_feature 1600..1605
/label=SDS
misc_feature 1871..1876
/label=SDS
misc_feature 1898..1903
/label=SDS
misc_feature 2903..2908
/label=SDS
CDS 3391..3456
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3460..3891
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3604..3609
/label=SDS
repeat_region 3934..4074
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3934..4063
/label=AAV2 ITR
repeat_region 3935..4075
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 4149..4604
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4886..4990
/gene="bla"
/label=AmpR promoter
CDS 4991..5851
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6022..6610
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacagggcc ccctgctcag
1561 ccccctggcc cgcccaacgc ctctagtaac cccgacctca ggaggagcga ccctggctgg
1621 gaacgctcgg acagcgtcct tccagcctct cacgggcacc tcccccaggc tggctcactg
1681 gagcggaacc gcgtgggagc ctcctccaaa ctggacagct cccctgtgct ctcccctggg
1741 aataaagcca agcccgacga ccaccgctca cggccaggcc ggcccgcaga ctttgtgttg
1801 ctgaaagagc ggactctgga cgaggcccct cggcctccca agaaggccat ggactactcg
1861 tcgtccagcg aggaggtgga aagcagtgag gacgacgagg aggaaggcga aggcgggcca
1921 gcagagggga gcagagatac ccctgggggc cgcagcgatg gggatacaga cagcgtcagc
1981 accatggtgg tccacgacgt cgaggagatc accgggaccc agcccccata cgggggcggc
2041 accatggtgg tccagcgcac ccctgaagag gagcggaacc tgctgcatgc tgacagcaat
2101 gggtacacaa acctgcctga cgtggtccag cccagccact cacccaccga gaacagcaaa
2161 ggccaaagcc caccctcgaa ggatgggagt ggtgactacc agtctcgtgg gctggtaaag
2221 gcccctggca agagctcgtt cacgatgttt gtggatctag ggatctacca gcctggaggc
2281 agtggggaca gcatccccat cacagcccta gtgggtggag agggcactcg gctcgaccag
2341 ctgcagtacg acgtgaggaa gggttctgtg gtcaacgtga atcccaccaa cacccgggcc
2401 cacagtgaga cccctgagat ccggaagtac aagaagcgat tcaactccga gatcctctgt
2461 gcagcccttt ggggggtcaa cctgctggtg ggcacggaga acgggctgat gttgctggac
2521 cgaagtgggc agggcaaggt gtatggactc attgggcggc gacgcttcca gcagatggat
2581 gtgctggagg ggctcaacct gctcatcacc atctcaggga aaaggaacaa actgcgggtg
2641 tattacctgt cctggctccg gaacaagatt ctgcacaatg acccagaagt ggagaagaag
2701 cagggctgga ccaccgtggg ggacatggag ggctgcgggc actaccgtgt tgtgaaatac
2761 gagcggatta agttcctggt catcgccctc aagagctccg tggaggtgta tgcctgggcc
2821 cccaaaccct accacaaatt catggccttc aagtcctttg ccgacctccc ccaccgccct
2881 ctgctggtcg acctgacagt agaggagggg cagcggctca aggtcatcta tggctccagt
2941 gctggcttcc atgctgtgga tgtcgactcg gggaacagct atgacatcta catccctgtg
3001 cacatccaga gccagatcac gccccatgcc atcatcttcc tccccaacac cgacggcatg
3061 gagatgctgc tgtgctacga ggacgagggt gtctacgtca acacgtacgg gcgcatcatt
3121 aaggatgtgg tgctgcagtg gggggagatg cctacttctg tggcctacat ctgctccaac
3181 cagataatgg gctggggtga gaaagccatt gagatccgct ctgtggagac gggccacctc
3241 gacggggtct tcatgcacaa acgagctcag aggctcaagt tcctgtgtga gcggaatgac
3301 aaggtgtttt ttgcctcagt ccgctctggg ggcagcagcc aagtttactt catgactctg
3361 aaccgtaact gcatcatgaa ctggtgtaca gactacaaag accatgacgg tgattataaa
3421 gatcatgaca tcgactacaa ggatgacgat gacaagtaac tcgagataat caacctctgg
3481 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
3541 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
3601 tctcctcctt gtataaatcc tggttagttc ttgccacggc ggaactcatc gccgcctgcc
3661 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgtttattt
3721 gtgaaatttg tgatgctatt gctttatttg taaccatcta gctttatttg tgaaatttgt
3781 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
3841 tgcattcatt ttatgtttca ggttcagggg gagatgtggg aggtttttta aagatctgga
3901 gaccggtgtg gcacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca
3961 ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc
4021 cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc
4081 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca
4141 accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
4201 cgtgaccgct acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt
4261 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
4321 ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg
4381 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
4441 taatagtgga ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt
4501 tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca
4561 aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag
4621 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
4681 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
4741 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
4801 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
4861 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
4921 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
4981 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
5041 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
5101 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
5161 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
5221 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
5281 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
5341 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
5401 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
5461 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
5521 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
5581 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
5641 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
5701 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
5761 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
5821 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
5881 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
5941 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
6001 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
6061 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
6121 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
6181 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
6241 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
6301 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
6361 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
6421 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
6481 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
6541 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
6601 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
6661 tgctcacatg t
//
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