pBAV1k-T5-LUX

LOCUS       pBAV1k-T5-LUX                8599 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expresses the Photorhabdus luminescens lux operon in bacterial 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    pBAV1k-T5-LUX
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8599)
  AUTHORS   Bryksin AV, Matsumura I
  TITLE     Rational design of a plasmid origin that replicates efficiently in 
            both gram-positive and gram-negative bacteria.
  JOURNAL   PLoS One. 2010  . 5(10):e13244.
  PUBMED    20949038
REFERENCE   2  (bases 1 to 8599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8599
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    14..30
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             40..51
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             58..1140
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     RBS             1152..1163
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1170..2153
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     RBS             2165..2176
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2183..3625
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPESDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MRHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     RBS             3637..3648
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             3655..4578
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PDNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     RBS             4590..4601
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             4608..5720
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     misc_feature    5724..5744
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      5795..5881
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             7212..7223
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      7425..7519
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(7535..8329)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEEYRCVKDTEGMSPAKVYKLVGENEILYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEGYED
                     EQSPEKTIELYAECIRLFHSIDISDCPYTNSLDGRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    8535..8556
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        8561..6
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    8581..8597
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ataatagatt caattgtgag cggataacaa ttactagaga aagaggagaa atactagatg
       61 aaatttggaa actttttgct tacataccaa cctccccaat tttctcaaac agaggtaatg
      121 aaacgtttgg ttaaattagg tcgcatctct gaggagtgtg gttttgatac cgtatggtta
      181 ctggagcatc atttcacgga gtttggtttg cttggtaacc cttatgtcgc tgctgcatat
      241 ttacttggcg cgactaaaaa attgaatgta ggaactgccg ctattgttct tcccacagcc
      301 catccagtac gccaacttga agatgtgaat ttattggatc aaatgtcaaa aggacgattt
      361 cggtttggta tttgccgagg gctttacaac aaggactttc gcgtattcgg cacagatatg
      421 aataacagtc gcgccttagc ggaatgctgg tacgggctga taaagaatgg catgacagag
      481 ggatatatgg aagctgataa tgaacatatc aagttccata aggtaaaagt aaaccccgcg
      541 gcgtatagca gaggtggcgc accggtttat gtggtggctg aatcagcttc gacgactgag
      601 tgggctgctc aatttggcct accgatgata ttaagttgga ttataaatac taacgaaaag
      661 aaagcacaac ttgagcttta taatgaagtg gctcaagaat atgggcacga tattcataat
      721 atcgaccatt gcttatcata tataacatct gtagatcatg actcaattaa agcgaaagag
      781 atttgccgga aatttctggg gcattggtat gattcttatg tgaatgctac gactattttt
      841 gatgattcag accaaacaag aggttatgat ttcaataaag ggcagtggcg tgactttgta
      901 ttaaaaggac ataaagatac taatcgccgt attgattaca gttacgaaat caatcccgtg
      961 ggaacgccgc aggaatgtat tgacataatt caaaaagaca ttgatgctac aggaatatca
     1021 aatatttgtt gtggatttga agctaatgga acagtagacg aaattattgc ttccatgaag
     1081 ctcttccagt ctgatgtcat gccatttctt aaagaaaaac aacgttcgct attatattaa
     1141 taatactaga gaaagaggag aaatactaga tgaaatttgg attgttcttc cttaacttca
     1201 tcaattcaac aactgttcaa gaacaaagta tagttcgcat gcaggaaata acggagtatg
     1261 ttgataagtt gaattttgaa cagattttag tgtatgaaaa tcatttttca gataatggtg
     1321 ttgtcggcgc tcctctgact gtttctggtt ttctgctcgg tttaacagag aaaattaaaa
     1381 ttggttcatt aaatcacatc attacaactc atcatcctgt ccgcatagcg gaggaagctt
     1441 gcttattgga tcagttaagt gaagggagat ttattttagg gtttagtgat tgcgaaaaaa
     1501 aagatgaaat gcattttttt aatcgcccgg ttgaatatca acagcaacta tttgaagagt
     1561 gttatgaaat cattaacgat gctttaacaa caggctattg taatccagat aacgattttt
     1621 atagcttccc taaaatatct gtaaatcccc atgcttatac gccaggcgga cctcggaaat
     1681 atgtaacagc aaccagtcat catattgttg agtgggcggc caaaaaaggt attcctctca
     1741 tctttaagtg ggatgattct aatgatgtta gatatgaata tgctgaaaga tataaagccg
     1801 ttgcggataa atatgacgtt gacctatcag agatagacca tcagttaatg atattagtta
     1861 actataacga agatagtaat aaagctaaac aagagacgcg tgcatttatt agtgattatg
     1921 ttcttgaaat gcaccctaat gaaaatttcg aaaataaact tgaagaaata attgcagaaa
     1981 acgctgtcgg aaattatacg gagtgtataa ctgcggctaa gttggcaatt gaaaagtgtg
     2041 gtgcgaaaag tgtattgctg tcctttgaac caatgaatga tttgatgagc caaaaaaatg
     2101 taatcaatat tgttgatgat aatattaaga agtaccacat ggaatatacc taataatact
     2161 agagaaagag gagaaatact agatgactaa aaaaatttca ttcattatta acggccaggt
     2221 tgaaatcttt cccgaaagtg atgatttagt gcaatccatt aattttggtg ataatagtgt
     2281 ttacctgcca atattgaatg actctcatgt aaaaaacatt attgattgta atggaaataa
     2341 cgaattacgg ttgcataaca ttgtcaattt tctctatacg gtagggcaaa gatggaaaaa
     2401 tgaagaatac tcaagacgca ggacatacat tcgtgactta aaaaaatata tgggatattc
     2461 agaagaaatg gctaagctag aggccaattg gatatctatg attttatgtt ctaaaggcgg
     2521 cctttatgat gttgtagaaa atgaacttgg ttctcgccat atcatggatg aatggctacc
     2581 tcaggatgaa agttatgttc gggcttttcc gaaaggtaaa tctgtacatc tgttggcagg
     2641 taatgttcca ttatctggga tcatgtctat attacgcgca attttaacta agaatcagtg
     2701 tattataaaa acatcgtcaa ccgatccttt taccgctaat gcattagcgt taagttttat
     2761 tgatgtagac cctaatcatc cgataacgcg ctctttatct gttatatatt ggccccacca
     2821 aggtgataca tcactcgcaa aagaaattat gcgacatgcg gatgttattg tcgcttgggg
     2881 agggccagat gcgattaatt gggcggtaga gcatgcgcca tcttatgctg atgtgattaa
     2941 atttggttct aaaaagagtc tttgcattat cgataatcct gttgatttga cgtccgcagc
     3001 gacaggtgcg gctcatgatg tttgttttta cgatcagcga gcttgttttt ctgcccaaaa
     3061 catatattac atgggaaatc attatgagga atttaagtta gcgttgatag aaaaacttaa
     3121 tctatatgcg catatattac cgaatgccaa aaaagatttt gatgaaaagg cggcctattc
     3181 tttagttcaa aaagaaagct tgtttgctgg attaaaagta gaggtggata ttcatcaacg
     3241 ttggatgatt attgagtcaa atgcaggtgt ggaatttaat caaccacttg gcagatgtgt
     3301 gtaccttcat cacgtcgata atattgagca aatattgcct tatgttcaaa aaaataagac
     3361 gcaaaccata tctatttttc cttgggagtc atcatttaaa tatcgagatg cgttagcatt
     3421 aaaaggtgcg gaaaggattg tagaagcagg aatgaataac atatttcgag ttggtggatc
     3481 tcatgacgga atgagaccgt tgcaacgatt agtgacatat atttctcatg aaaggccatc
     3541 taactatacg gctaaggatg ttgcggttga aatagaacag actcgattcc tggaagaaga
     3601 taagttcctt gtatttgtcc cataataata ctagagaaag aggagaaata ctagatggaa
     3661 aatgaatcaa aatataaaac catcgaccac gttatttgtg ttgaaggaaa taaaaaaatt
     3721 catgtttggg aaacgctgcc agaagaaaac agcccaaaga gaaagaatgc cattattatt
     3781 gcgtctggtt ttgcccgcag gatggatcat tttgctggtc tggcggaata tttatcgcgg
     3841 aatggatttc atgtgatccg ctatgattcg cttcaccacg ttggattgag ttcagggaca
     3901 attgatgaat ttacaatgtc tataggaaag cagagcttgt tagcagtggt tgattggtta
     3961 actacacgaa aaataaataa cttcggtatg ttggcttcaa gcttatctgc gcggatagct
     4021 tatgcaagcc tatctgaaat caatgcttcg tttttaatca ccgcagtcgg tgttgttaac
     4081 ttaagatatt ctcttgaaag agctttaggg tttgattatc tcagtctacc cattaatgaa
     4141 ttgccggata atctggattt tgaaggccat aaattgggtg ctgaagtctt tgcgagagat
     4201 tgtcttgatt ttggttggga agatttagct tctacaatta ataacatgat gtatcttgat
     4261 ataccgttta ttgcttttac tgcaaataac gataattggg tcaagcaaga tgaagttatc
     4321 acattgttat caaatattcg tagtaatcga tgcaagatat attctttgtt aggaagttcg
     4381 catgacttga gtgaaaattt agtggtcctg cgcaattttt atcaatcggt tacgaaagcc
     4441 gctatcgcga tggataatga tcatctggat attgatgttg atattactga accgtcattt
     4501 gaacatttaa ctattgcgac agtcaatgaa cgccgaatga gaattgagat tgaaaatcaa
     4561 gcaatttctc tgtcttaata atactagaga aagaggagaa atactagatg acttcatatg
     4621 ttgataaaca agaaattaca gcaagctcag aaattgatga tttgattttt tcgagcgatc
     4681 cattagtgtg gtcttacgac gagcaggaaa aaatcagaaa gaaacttgtg cttgatgcat
     4741 ttcgtaatca ttataaacat tgtcgagaat atcgtcacta ctgtcaggca cacaaagtag
     4801 atgacaatat tacggaaatt gatgacatac ctgtattccc aacatcggtt tttaagttta
     4861 ctcgcttatt aacttctcag gaaaacgaga ttgaaagttg gtttaccagt agcggcacga
     4921 atggtttaaa aagtcaggtg gcgcgtgaca gattaagtat tgagagactc ttaggctctg
     4981 tgagttatgg catgaaatat gttggtagtt ggtttgatca tcaaatagaa ttagtcaatt
     5041 tgggaccaga tagatttaat gctcataata tttggtttaa atatgttatg agtttggtgg
     5101 aattgttata tcctacgaca tttaccgtaa cagaagaacg aatagatttt gttaaaacat
     5161 tgaatagtct tgaacgaata aaaaatcaag ggaaagatct ttgtcttatt ggttcgccat
     5221 actttattta tttactctgc cattatatga aagataaaaa aatctcattt tctggagata
     5281 aaagccttta tatcataacc ggaggcggct ggaaaagtta cgaaaaagaa tctctgaaac
     5341 gtgatgattt caatcatctt ttatttgata ctttcaatct cagtgatatt agtcagatcc
     5401 gagatatatt taatcaagtt gaactcaaca cttgtttctt tgaggatgaa atgcagcgta
     5461 aacatgttcc gccgtgggta tatgcgcgag cgcttgatcc tgaaacgttg aaacctgtac
     5521 ctgatggaac gccggggttg atgagttata tggatgcgtc agcaaccagt tatccagcat
     5581 ttattgttac cgatgatgtc gggataatta gcagagaata tggtaagtat cccggcgtgc
     5641 tcgttgaaat tttacgtcgc gtcaatacga ggacgcagaa agggtgtgct ttaagcttaa
     5701 ccgaagcgtt tgatagttaa taatactagt agcggccgct gcaggcctca gggcccgatc
     5761 gatgccgccg cttaattaat taatccagag gcatcaaata aaacgaaagg ctcagtcgaa
     5821 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa
     5881 tccgccgccc tagacctagt gtcattttat ttcccccgtt tcagcatcaa gaacctttgc
     5941 ataacttgct ctatatccac actgataatt gccctcaaac cataatctaa aggcgctaga
     6001 gtttgttgaa acaatatctt ttacatcatt cgtatttaaa attccaaact ccgctcccct
     6061 aaggcgaata aaagccatta aatcttttgt atttaccaaa ttatagtcat ccactatatc
     6121 taagagtaaa ttcttcaatt ctcttttttg gctttcatca agtgttatat agcggtcaat
     6181 atcaaaatca ttaatgttca aaatatcttt tttgtcgtat atatgtttat tcttagcaat
     6241 agcgtccttt gattcatgag tcaaatattc atatgaacct ttgatataat caagtatctc
     6301 aacatgagca actgaactat tccccaattt tcgcttaatc ttgttcctaa cgctttctat
     6361 tgttacagga tttcgtgcaa tatatataac gtgatagtgt ggttttttat agtgctttcc
     6421 atttcgtata acatcactac tattccatgt atctttatct tttttttcgt ccatatcgtg
     6481 taaaggactg acagccatag atacgcccaa actctctaat ttttccttcc aatcattagg
     6541 aattgagtca ggatataata aaaatccaaa atttctagct ttagtatttt taatagccat
     6601 gatataatta ccttatcaaa aacaagtagc gaaaactcgt atccttctaa aaacgcgagc
     6661 tttcgcttat tttttttgtt ctgattcctt tcttgcatat tcttctatag ctaacgccgc
     6721 aaccgcagat tttgaaaaac ctttttgttt cgccatatct gttaattttt tatcttgctc
     6781 ttttgtcaga gaaatcataa ctcttttttt cgattctgaa atcaccattt aaaaaactcc
     6841 aatcaaataa ttttataaag ttagtgtatc actttgtaat cataaaaaca acaataaagc
     6901 tacttaaata tagatttata aaaaacgttg gcgaaaacgt tggcgattcg ttggcgattg
     6961 aaaaacccct taaacccttg agccagttgg gatagagcgt ttttggcaca aaaattggca
     7021 ctcggcactt aatggggggt cgtagtacgg aagcaaaatt cgcttccttt ccccccattt
     7081 ttttccaaat tccagatttt tttcaaaaat tttccagcgc taccgctcgg caaaattgca
     7141 agcaattttt aaaatcaaac ccatgaggga atttcattcc ctcatactcc cttgagcctc
     7201 ctccaaccga aatagaaggg cgctgcgctt attatttcat tcagtcatcg gctttcataa
     7261 tctaacagac aacatcttcg ctgcaaagcc acgctacgct caagggcttt tacgctacga
     7321 taacgcctgt tttaacgatt atgccgataa ctaaacgaaa taaacgctaa aacgtctcag
     7381 aaacgatttt gagacgtttt aataaaaaat cgcctagtgc ttggattctc accaataaaa
     7441 aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact
     7501 ggatctatca acgggagtcc aagcgagctc ggtactaaaa caattcatcc agtaaaatat
     7561 aatattttat tttctcccaa tcaggcttga tccccagtaa gtcaaaaaat agctcgacat
     7621 actgttcttc cccgatatcc tccctgatcg accggacgca gaaggcaatg tcataccact
     7681 tgtccgccct gccgcttctc ccaagatcaa taaagccact tactttgcca tctttcacaa
     7741 agatgttgct gtctcccagg tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg
     7801 tctttaaaaa atcatacagc tcgcgcggat ctttaaatgg agtgtcttct tcccagtttt
     7861 cgcaatccac atcggccaga tcgttattca gtaagtaatc caattcggct aagcggccgt
     7921 ctaagctatt cgtataggga caatccgata tgtcgatgga gtgaaagagc ctgatgcact
     7981 ccgcatacag ctcgatagtc ttttcagggc tttgttcatc ttcataccct tccgagcaaa
     8041 ggacgccatc ggcctcactc atgagcagat tgctccagcc atcatgccgt tcaaagtgca
     8101 ggacctttgg aacaggcagc tttccttcca gccatagcat catgtccttt tcccgttcca
     8161 catcataggt ggtcccttta taccggctgt ccgtcatttt taaatatagg atttcatttt
     8221 ctcccaccag cttatatacc ttagcaggag acattccttc cgtatctttt acgcagcggt
     8281 attcttcgat cagttttttc aattccggtg atattctcat tttagccatt tattatttcc
     8341 ttcctctttt ctacagtatt taaagatacc ccaagaagct aattataaca agacgaactc
     8401 caattcactg ttccttgcat tctaaaacct taaatacaga aaacagcctt ttcaaagttg
     8461 ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc acaattatga
     8521 tagaatttga cgtcgaattc gcggccgctt ctagaggaaa tcataaaaaa tttatttgct
     8581 ttgtgagcgg ataacaatt
//