| 质粒名称: | pEGFP-NUP98 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000287 |
| 产品用途: | Expresses EGFP-NUP28 in mammalian cells culture |
| 存储实验室: | KIRYL PIATKEVICH Lab |
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载体骨架基本信息 |
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| 载体名称: | pN1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
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插入基因信息 |
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| 插入基因名称: | EGFP-NUP98 |
| 物种来源: | Synthetic, synthetic |
| 密码子优化: | 哺乳动物表达优化 |
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文献引用方法 |
|
| 参考文献部分: |
Bright and stable monomeric green fluorescent protein derived from StayGold
Zhang H., Lesnov G.D., Subach O.M., Zhang W., Kuzmicheva T.P., Vlaskina A.V., Samygina V.R., Chen L., Ye X., Nikolaeva A.Y., Gabdulkhakov A., Papadaki S., Qin W., Borshchevskiy V., Perfilov M.M., Gavrikov A.S., Drobizhev M., Mishin A.S., Piatkevich K.D., Subach F.V.
Nat. Methods 2024 214 21, 657–665 (2024)
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pEGFP-NUP98
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
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pEGFP-NUP98载体质粒基本信息
pEGFP-NUP98质粒图谱载体图谱和pEGFP-NUP98载体序列质粒序列多克隆位点信息
pEGFP-NUP98质粒载体简介
pEGFP-NUP98质粒序列载体序列
LOCUS pEGFP-NUP98 7509 bp DNA circular SYN 21-NOV-2023 DEFINITION Cloning vector pEGFP-C2 with enhanced green fluorescent protein gene, complete sequence. ACCESSION U57606 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7509) AUTHORS Cormack BP, Valdivia RH, Falkow S. TITLE FACS-optimized mutants of the green fluorescent protein (GFP) JOURNAL Gene 173 (1 SPEC NO), 33-38 (1996) PUBMED 8707053 REFERENCE 2 (bases 1 to 7509) AUTHORS Kitts PA. TITLE pEGFP-C2 complete sequence JOURNAL Unpublished REFERENCE 3 (bases 1 to 7509) AUTHORS Kitts PA. TITLE Direct Submission JOURNAL Submitted (06-MAY-1996) P.A. Kitts, CLONTECH Laboratories, Inc., 1020 East Meadow Circle, Palo Alto, CA 94303-4230, USA REFERENCE 4 (bases 1 to 7509) AUTHORS . TITLE Direct Submission JOURNAL Exported Jul 5, 2026 from SnapGene 6.0.2 https://www.biofeng.com COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Gene 173 (1 SPEC NO), 33-38 (1996)" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 3; type: "Journal Article"; journalName: "Submitted (06-MAY-1996) P.A. Kitts, CLONTECH Laboratories, Inc., 1020 East Meadow Circle, Palo Alto, CA 94303-4230, USA" COMMENT This vector can be obtained from CLONTECH Laboratories, Inc., 1020 East Meadow Circle, Palo Alto, CA 94303-4230, USA. To place an order call (415) 424-8222 or (800) 662-2566, extension 1. International customers, please contact your local distributor. For technical information, call (415) 424-8222 or (800) 662-2566, extension 3. This sequence has been compiled from information in the sequence databases, published literature and other sources, together with partial sequences obtained by CLONTECH. If you suspect there is an error in this sequence, please contact CLONTECH's Technical Service Department at (415) 424-8222 or (800) 662-2566, extension 3 or E-mail TECH@CLONTECH.COM. FEATURES Location/Qualifiers source 1..7509 /mol_type="other DNA" /db_xref="taxon:47840" /organism="Cloning vector pEGFP-C2" enhancer 61..364 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 365..568 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" misc_feature 590..612 /label=Feature 12 regulatory 607..616 /label=Kozak sequence /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" gene 613..1353 /gene="egfp" /label=egfp CDS 613..1329 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind 1278..1298 /label=EGFP-F misc_feature 1343..1353 /label=multiple cloning site /note="multiple cloning site" misc_feature 1343..1353 /label=MCS /note="multiple cloning site" CDS 1354..4164 /codon_start=1 /label=NUP98 and 96 /translation="MFNKSFGTPFGGGTGGFGTTSTFGQNTGFGTTSGGAFGTSAFGSS NNTGGLFGNSQTKPGGLFGTSSFSQPATSTSTGFGFGTSTGTANTLFGTASTGTSLFSS QNNAFAQNKPTGFGNFGTSTSSGGLFGTTNTTSNPFGSTSGSLFGPSSFTAAPTGTTIK FNPPTGTDTMVKAGVSTNISTKHQCITAMKEYESKSLEELRLEDYQANRKGPQNQVGAG TTTGLFGSSPATSSATGLFSSSTTNSGFAYGQNKTAFGTSTTGFGTNPGGLFGQQNQQT TSLFSKPFGQATTTQNTGFSFGNTSTIGQPSTNTMGLFGVTQASQPGGLFGTATNTSTG TAFGTGTGLFGQTNTGFGAVGSTLFGNNKLTTFGSSTTSAPSFGTTSGGLFGNKPTLTL GTNTNTSNFGFGTNTSGNSIFGSKPAPGTLGTGLGAGFGTALGAGQASLFGNNQPKIGG PLGTGAFGAPGFNTTTATLGFGAPQAPVALTDPNASAAQQAVLQQHINSLTYSPFGDSP LFRNPMSDPKKKEERLKPTNPAAQKALTTPTHYKLTPRPATRVRPKALQTTGTAKSHLF DGLDDDEPSLANGAFMPKKSIKKLVLKNLNNSNLFSPVNRDSENLASPSEYPENGERFS FLSKPVDENHQQDGDEDSLVSHFYTNPIAKPIPQTPESAGNKHSNSNSVDDTIVALNMR AALRNGLEGSSEETSFHDESLQDDREEIENNSYHMHPAGIILTKVGYYTIPSMDDLAKI TNEKGECIVSDFTIGRKGYGSIYFEGDVNLTNLNLDDIVHIRRKEVVVYLDDNQKPPVG EGLNRKAEVTLDGVWPTDKTSRCLIKSPDRLADINYEGRLEAVSRKQGAQFKEYRPETG SWVFKVSHFSKYGLQDSDEEEEEHPSKTSTKKLKTAPLPPASQTTPLQMALNGKPAPPP QVEKKGQ" primer_bind 1649..1665 /label=MCS-F primer_bind 2011..2030 /label=NUP98-Fseq primer_bind 2449..2474 /label=MCS-F2 polyA_signal 4297..4418 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin complement(4425..4880) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 4907..5011 /gene="bla" /label=AmpR promoter promoter 5013..5370 /label=SV40 promoter /note="SV40 enhancer and early promoter" CDS 5405..6199 /codon_start=1 /product="neomycin phosphotransferase" /label=neomycin phosphotransferase /protein_id="AAB08059.1" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" polyA_signal 6431..6478 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 6807..7395 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg 781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc 841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac 1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc 1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc 1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 1321 ctgtacaagt ccggccggac tcagatctcg agcatgttta acaaatcatt tggaacaccc 1381 tttgggggtg gcacaggtgg ctttggcaca acttcaacat ttggacagaa tactggcttt 1441 ggcactacta gtggaggggc atttggaaca tctgcatttg gttctagcaa caatactgga 1501 ggcctctttg gaaattcaca gactaaacca ggaggattgt ttggaaccag ttcatttagc 1561 cagccagcta cctccacaag cactggcttt gggtttggta cgtcaacagg aacagcaaat 1621 accttgtttg gaactgcaag cacagggacc agtctcttct catcccaaaa caatgccttt 1681 gcacaaaata aaccaactgg ctttggcaat tttggaacca gtactagcag tggaggactc 1741 tttggaacca caaataccac ctctaatcct tttggcagca catctggctc cctctttggg 1801 ccaagtagtt ttacagctgc tcctactggg actactatta aatttaaccc tccaactggt 1861 acagatacta tggtcaaagc tggagttagc actaacataa gtaccaagca ccagtgtatt 1921 actgctatga aagaatatga aagcaagtca ctagaggaac ttcgtttaga ggattatcag 1981 gctaacagga agggcccaca gaaccaggtg ggagcaggta ccacaactgg cttgtttggg 2041 tcttctccag ccacttccag cgcaacagga ctcttcagct cctccaccac taattcaggc 2101 tttgcatatg gtcagaacaa aactgccttt ggaactagta caactggatt tggaacaaat 2161 ccaggtggtc tctttggcca acagaatcag cagactacca gcctcttcag caaaccattt 2221 ggccaggcta caaccaccca gaacactggc ttttcctttg gtaataccag caccatagga 2281 cagccaagca ccaacaccat gggattattt ggagtaaccc aagcctcaca gcctggaggt 2341 ctttttggga cagctacaaa caccagcact gggacagcat ttggaacagg aacaggtctc 2401 tttgggcaga ccaatactgg atttggtgct gttggttcga ccctgtttgg caataacaag 2461 cttactacat ttggaagcag cacaaccagt gcaccttcat ttggtacaac cagtggcggg 2521 ctctttggta acaaaccaac cctgacttta ggaaccaata caaacacttc taattttggt 2581 tttggcacaa ataccagtgg gaatagtatt tttggaagta aaccagcacc tgggactctt 2641 ggaactgggc ttggtgcagg atttggaaca gctcttggtg ctggacaggc atctttgttt 2701 gggaacaacc aacctaagat tggagggcct cttggtacag gagcctttgg ggcccctgga 2761 tttaatacta cgacagccac tttgggcttt ggagcccccc aggccccagt agctttgaca 2821 gatccaaatg cttctgctgc ccagcaggct gttctccagc agcacatcaa tagtctaaca 2881 tactcacctt ttggagactc tcctctcttc cggaatccga tgtcagaccc taagaagaag 2941 gaagagagat tgaaaccaac aaatccagca gcccagaagg ctcttactac acctactcat 3001 tataaactga caccccgccc tgccactaga gtccggccaa aggctttaca aacaacaggc 3061 acagccaagt cacatctctt tgatgggctg gatgacgatg aaccatccct agccaatgga 3121 gcattcatgc ccaagaagag cattaagaag ttggttttga agaaccttaa taatagcaat 3181 ctcttttctc ctgttaatcg tgattcagaa aatctagctt caccatctga atatccagaa 3241 aatggagaga gatttagttt cctaagcaaa cctgttgatg agaatcacca gcaggatgga 3301 gatgaagatt cccttgtttc acatttttat actaacccta ttgccaaacc tattcctcaa 3361 accccagaaa gtgctggaaa taaacacagc aacagcaaca gtgtggatga taccattgtt 3421 gcattaaaca tgcgtgctgc tttgcgaaat gggctggaag gaagcagtga agaaacgtct 3481 tttcatgatg agtcacttca ggatgaccga gaagaaatag aaaataattc ttaccatatg 3541 cacccagcag gtattattct cactaaggtt ggttactata ctattccatc tatggatgac 3601 cttgctaaaa ttaccaatga aaaaggagag tgcattgtct ctgatttcac tattggtcgg 3661 aaaggttatg gttcaatcta ttttgaagga gatgtgaatt tgacaaatct aaatttggat 3721 gatattgtgc atatccggag gaaagaagta gttgtctact tagatgataa ccaaaaacca 3781 cctgtgggtg aagggctaaa taggaaggct gaagttacat tggatggagt ttggccaaca 3841 gataaaacat ctcgttgttt aataaagagc ccagatcgcc ttgctgatat caactatgaa 3901 ggaagattgg aagcagtttc aaggaaacag ggagctcaat tcaaagaata ccggcctgaa 3961 actggttctt gggtgtttaa ggtctcccat ttttctaagt atggccttca ggattctgat 4021 gaagaggagg aggagcatcc gtctaaaact agtacaaaga agttgaagac tgctcctttg 4081 cctcctgcaa gccagactac gcccttgcag atggctctta atggcaaacc tgcacctcca 4141 cctcaggtag agaaaaaagg acagtgagga tccaccggat ctagataact gatcataatc 4201 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg 4261 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat 4321 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat 4381 tctagttgtg gtttgtccaa actcatcaat gtatcttaac gcgtaaattg taagcgttaa 4441 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc 4501 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt 4561 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa 4621 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg 4681 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg 4741 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc 4801 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa 4861 tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat 4921 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata 4981 aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat 5041 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc 5101 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga 5161 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc 5221 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 5281 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga 5341 ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt 5401 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct 5461 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct 5521 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga 5581 actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc 5641 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg 5701 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc 5761 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca 5821 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga 5881 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc 5941 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga 6001 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca 6061 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg 6121 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct 6181 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc 6241 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga 6301 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc 6361 ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc 6421 cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat 6481 aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg 6541 ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc 6601 ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat 6661 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 6721 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 6781 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 6841 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 6901 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta 6961 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 7021 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 7081 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 7141 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 7201 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 7261 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 7321 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 7381 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 7441 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc 7501 gccatgcat //
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pEGFP-NUP98质粒载体应用举例
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