pAAVLINKv1-pCALM1-Cre-3'-SCN9A-FLAG

LOCUS       pAAVLINKv1-pCALM1-Cre-3'-SCN9A-FLAG                7215 bp DNA     circular SYN 10-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7215)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 12, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7215
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     promoter        145..265
                     /label=pCALM1
     CDS             277..1308
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     intron          1309..1349
                     /label=Splicing Donor
     protein_bind    1356..1389
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1390..1481
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1482..3928
                     /codon_start=1
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; 
                     NE-NA;NENA; PN1; SFNP"
                     /product="sodium channel protein type 9 subunit 
                     alphaisoform 2"
                     /label=SCN9A
                     /note="isoform 2 is encoded by transcript variant 2;sodium 
                     channel, voltage-gated, type IX, alpha polypeptide; sodium 
                     channel protein type 9 subunit alpha; sodium channel, 
                     voltage-gated, type IX, alpha subunit; voltage-gated sodium
                     channel alpha subunit Nav1.7; hNE-Na; peripheral sodium 
                     channel 1; neuroendocrine sodium channel; sodium channel 
                     protein type IX subunit alpha; voltage-gated sodium channel
                     subunit alpha Nav1.7"
                     /db_xref="CCDS:CCDS92892.1"
                     /db_xref="GeneID:6335"
                     /db_xref="HGNC:HGNC:10597"
                     /db_xref="MIM:603415"
                     /protein_id="NP_001352465.1"
                     /translation="KGKIWWNIRKTCYKIVEHSWFESFIVLMILLSSGALAFEDIYIER
                     KKTIKIILEYADKIFTYIFILEMLLKWIAYGYKTYFTNAWCWLDFLIVDVSLVTLVANT
                     LGYSDLGPIKSLRTLRALRPLRALSRFEGMRVVVNALIGAIPSIMNVLLVCLIFWLIFS
                     IMGVNLFAGKFYECINTTDGSRFPASQVPNRSECFALMNVSQNVRWKNLKVNFDNVGLG
                     YLSLLQVATFKGWTIIMYAAVDSVNVDKQPKYEYSLYMYIYFVVFIIFGSFFTLNLFIG
                     VIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKIQGCIFDLVT
                     NQAFDISIMVLICLNMVTMMVEKEGQSQHMTEVLYWINVVFIILFTGECVLKLISLRHY
                     YFTVGWNIFDFVVVIISIVGMFLADLIETYFVSPTLFRVIRLARIGRILRLVKGAKGIR
                     TLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKKEDGINDMFNFETFGNSMIC
                     LFQITTSAGWDGLLAPILNSKPPDCDPKKVHPGSSVEGDCGNPSVGIFYFVSYIIISFL
                     VVVNMYIAVILENFSVATEESTEPLSEDDFEMFYEVWEKFDPDATQFIEFSKLSDFAAA
                     LDPPLLIAKPNKVQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDSLRSQMEERF
                     MSANPSKVSYEPITTTLKRKQEDVSATVIQRAYRRYRLRQNVKNISSIYIKDGDRDDDL
                     LNKKDMAFDNVNENSSPEKTDATSSTTSPPSYDSVTKPDKEKYEQDRTEKEDKGKDSKE
                     SKK"
     misc_feature    2571..2576
                     /label=SDS
     misc_feature    2908..2913
                     /label=SDS
     misc_feature    3395..3400
                     /label=SDS
     CDS             3935..4000
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      4004..4435
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    4148..4153
                     /label=SDS
     repeat_region   4478..4618
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4478..4607
                     /label=AAV2 ITR
     repeat_region   4479..4619
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4693..5148
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5430..5534
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5535..6395
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6566..7154
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
      181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
      241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
      301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
      361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
      421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
      481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
      541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
      601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
      661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
      721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
      781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
      841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
      901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
      961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
     1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
     1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
     1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
     1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
     1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
     1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
     1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
     1441 tattggtctt actgacatcc actttgcctt tctctccaca gggaaaggaa aaatctggtg
     1501 gaacatcagg aaaacctgct acaagattgt tgaacacagt tggtttgaaa gcttcattgt
     1561 cctcatgatc ctgctcagca gtggtgccct ggcttttgaa gatatttata ttgaaaggaa
     1621 aaagaccatt aagattatcc tggagtatgc agacaagatc ttcacttaca tcttcattct
     1681 ggaaatgctt ctaaaatgga tagcatatgg ttataaaaca tatttcacca atgcctggtg
     1741 ttggctggat ttcctaattg ttgatgtttc tttggttact ttagtggcaa acactcttgg
     1801 ctactcagat cttggcccca ttaaatccct tcggacactg agagctttaa gacctctaag
     1861 agccttatct agatttgaag gaatgagggt cgttgtgaat gcactcatag gagcaattcc
     1921 ttccatcatg aatgtgctac ttgtgtgtct tatattctgg ctgatattca gcatcatggg
     1981 agtaaatttg tttgctggca agttctatga gtgtattaac accacagatg ggtcacggtt
     2041 tcctgcaagt caagttccaa atcgttccga atgttttgcc cttatgaatg ttagtcaaaa
     2101 tgtgcgatgg aaaaacctga aagtgaactt tgataatgtc ggacttggtt acctatctct
     2161 gcttcaagtt gcaactttta agggatggac gattattatg tatgcagcag tggattctgt
     2221 taatgtagac aagcagccca aatatgaata tagcctctac atgtatattt attttgtcgt
     2281 ctttatcatc tttgggtcat tcttcacttt gaacttgttc attggtgtca tcatagataa
     2341 tttcaaccaa cagaaaaaga agcttggagg tcaagacatc tttatgacag aagaacagaa
     2401 gaaatactat aatgcaatga aaaagctggg gtccaagaag ccacaaaagc caattcctcg
     2461 accagggaac aaaatccaag gatgtatatt tgacctagtg acaaatcaag cctttgatat
     2521 tagtatcatg gttcttatct gtctcaacat ggtaaccatg atggtagaaa aggagggtca
     2581 aagtcaacat atgactgaag ttttatattg gataaatgtg gtttttataa tccttttcac
     2641 tggagaatgt gtgctaaaac tgatctccct cagacactac tacttcactg taggatggaa
     2701 tatttttgat tttgtggttg tgattatctc cattgtaggt atgtttctag ctgatttgat
     2761 tgaaacgtat tttgtgtccc ctaccctgtt ccgagtgatc cgtcttgcca ggattggccg
     2821 aatcctacgt ctagtcaaag gagcaaaggg gatccgcacg ctgctctttg ctttgatgat
     2881 gtcccttcct gcgttgttta acatcggcct cctgctcttc ctggtcatgt tcatctacgc
     2941 catctttgga atgtccaact ttgcctatgt taaaaaggaa gatggaatta atgacatgtt
     3001 caattttgag acctttggca acagtatgat ttgcctgttc caaattacaa cctctgctgg
     3061 ctgggatgga ttgctagcac ctattcttaa cagtaagcca cccgactgtg acccaaaaaa
     3121 agttcatcct ggaagttcag ttgaaggaga ctgtggtaac ccatctgttg gaatattcta
     3181 ctttgttagt tatatcatca tatccttcct ggttgtggtg aacatgtaca ttgcagtcat
     3241 actggagaat tttagtgttg ccactgaaga aagtactgaa cctctgagtg aggatgactt
     3301 tgagatgttc tatgaggttt gggagaagtt tgatcccgat gcgacccagt ttatagagtt
     3361 ctctaaactc tctgattttg cagctgccct ggatcctcct cttctcatag caaaacccaa
     3421 caaagtccag ctcattgcca tggatctgcc catggttagt ggtgaccgga tccattgtct
     3481 tgacatctta tttgctttta caaagcgtgt tttgggtgag agtggggaga tggattctct
     3541 tcgttcacag atggaagaaa ggttcatgtc tgcaaatcct tccaaagtgt cctatgaacc
     3601 catcacaacc acactaaaac ggaaacaaga ggatgtgtct gctactgtca ttcagcgtgc
     3661 ttatagacgt taccgcttaa ggcaaaatgt caaaaatata tcaagtatat acataaaaga
     3721 tggagacaga gatgatgatt tactcaataa aaaagatatg gcttttgata atgttaatga
     3781 gaactcaagt ccagaaaaaa cagatgccac ttcatccacc acctctccac cttcatatga
     3841 tagtgtaaca aagccagaca aagagaaata tgaacaagac agaacagaaa aggaagacaa
     3901 agggaaagac agcaaggaaa gcaaaaaatg tacagactac aaagaccatg acggtgatta
     3961 taaagatcat gacatcgact acaaggatga cgatgacaag taactcgaga taatcaacct
     4021 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     4081 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     4141 attttctcct ccttgtataa atcctggtta gttcttgcca cggcggaact catcgccgcc
     4201 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttt
     4261 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca tctagcttta tttgtgaaat
     4321 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     4381 caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt tttaaagatc
     4441 tggagaccgg tgtggcacgt gcggaccgag cggccgcagg aacccctagt gatggagttg
     4501 gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga
     4561 cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg
     4621 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa
     4681 agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     4741 gcagcgtgac cgctacactt gccagcgcct tagcgcccgc tcctttcgct ttcttccctt
     4801 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     4861 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt
     4921 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     4981 tctttaatag tggactcttg ttccaaactg gaacaacact caactctatc tcgggctatt
     5041 cttttgattt ataagggatt ttgccgattt cggtctattg gttaaaaaat gagctgattt
     5101 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc
     5161 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     5221 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     5281 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     5341 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     5401 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     5461 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     5521 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     5581 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     5641 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     5701 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     5761 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     5821 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     5881 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     5941 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     6001 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     6061 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     6121 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     6181 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     6241 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     6301 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     6361 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     6421 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     6481 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     6541 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     6601 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     6661 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     6721 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     6781 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     6841 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     6901 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     6961 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     7021 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     7081 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     7141 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     7201 cttttgctca catgt
//