| 质粒名称: | pAAVLINK-EF1a-HA-DSP-A |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001775 |
| 产品用途: | 5' AAVLINK plasmid for DSP expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | DSP |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-DSP-A
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-DSP-A载体质粒基本信息
pAAVLINK-EF1a-HA-DSP-A质粒图谱载体图谱和pAAVLINK-EF1a-HA-DSP-A载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-DSP-A质粒载体简介
pAAVLINK-EF1a-HA-DSP-A质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-DSP-A 6581 bp DNA circular SYN 24-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6581)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 8, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6581
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 146..688
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 175..386
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 399..667
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 493..498
/label=SDS
CDS 704..730
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 737..3721
/codon_start=1
/label=DSP
/translation="MSCNGGSHPRINTLGRMIRAESGPDLRYEVTSGGGGTSRMYYSRR
GVITDQNSDGYCQTGTMSRHQNQNTIQELLQNCSDCLMRAELIVQPELKYGDGIQLTRS
RELDECFAQANDQMEILDSLIREMRQMGQPCDAYQKRLLQLQEQMRALYKAISVPRVRR
ASSKGGGGYTCQSGSGWDEFTKHVTSECLGWMRQQRAEMDMVAWGVDLASVEQHINSHR
GIHNSIGDYRWQLDKIKADLREKSAIYQLEEEYENLLKASFERMDHLRQLQNIIQATSR
EIMWINDCEEEELLYDWSDKNTNIAQKQEAFSIRMSQLEVKEKELNKLKQESDQLVLNQ
HPASDKIEAYMDTLQTQWSWILQITKCIDVHLKENAAYFQFFEEAQSTEAYLKGLQDSI
RKKYPCDKNMPLQHLLEQIKELEKEREKILEYKRQVQNLVNKSKKIVQLKPRNPDYRSN
KPIILRALCDYKQDQKIVHKGDECILKDNNERSKWYVTGPGGVDMLVPSVGLIIPPPNP
LAVDLSCKIEQYYEAILALWNQLYINMKSLVSWHYCMIDIEKIRAMTIAKLKTMRQEDY
MKTIADLELHYQEFIRNSQGSEMFGDDDKRKIQSQFTDAQKHYQTLVIQLPGYPQHQTV
TTTEITHHGTCQDVNHNKVIETNRENDKQETWMLMELQKIRRQIEHCEGRMTLKNLPLA
DQGSSHHITVKINELKSVQNDSQAIAEVLNQLKDMLANFRGSEKYCYLQNEVFGLFQKL
ENINGVTDGYLNSLCTVRALLQAILQTEDMLKVYEARLTEEETVCLDLDKVEAYRCGLK
KIKNDLNLKKSLLATMKTELQKAQQIHSQTSQQYPLYDLDLGKFGEKVTQLTDRWQRID
KQIDFRLWDLEKQIKQLRNYRDNYQAFCKWLYDAKRRQDSLESMKFGDSNTVMRFLNEQ
KNLHSEISGKRDKSEEVQKIAELCANSIKDYELQLASYTSGLETLLNIPIKRTMIQSPS
GVILQE"
misc_feature 1491..1496
/label=SDS
misc_feature 1605..1610
/label=SDS
misc_feature 1608..1613
/label=SDS
misc_feature 1662..1667
/label=SDS
misc_feature 2273..2278
/label=SDS
misc_feature 3114..3119
/label=SDS
intron 3722..3762
/label=Splicing Donor
misc_feature 3766..3799
/label=loxP-JT1571
repeat_region 3844..3984
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3844..3973
/label=AAV2 ITR
rep_origin 4059..4514
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4796..4900
/gene="bla"
/label=AmpR promoter
CDS 4901..5761
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5932..6520
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgtaccctt atgacgtacc
721 tgactatgct ggatctatga gctgcaacgg aggctcccac ccgcggatca acactctggg
781 ccgcatgatc cgcgccgagt ctggcccgga cctgcgctac gaggtgacca gcggcggcgg
841 gggcaccagc aggatgtact attctcggcg cggcgtgatc accgaccaga actcggacgg
901 ctactgtcaa accggcacga tgtccaggca ccagaaccag aacaccatcc aggagctgct
961 gcagaactgc tccgactgct tgatgcgagc agagctcatc gtgcagcctg aattgaagta
1021 tggagatgga atacaactga ctcggagtcg agaattggat gagtgttttg cccaggccaa
1081 tgaccaaatg gaaatcctcg acagcttgat cagagagatg cggcagatgg gccagccctg
1141 tgatgcttac cagaaaaggc ttcttcagct ccaagagcaa atgcgagccc tttataaagc
1201 catcagtgtc cctcgagtcc gcagggccag ctccaagggt ggtggaggct acacttgtca
1261 gagtggctct ggctgggatg agttcaccaa acatgtcacc agtgaatgtt tggggtggat
1321 gaggcagcaa agggcggaga tggacatggt ggcctggggt gtggacctgg cctcagtgga
1381 gcagcacatt aacagccacc ggggcatcca caactccatc ggcgactatc gctggcagct
1441 ggacaaaatc aaagccgacc tgcgcgagaa atctgcgatc taccagttgg aggaggagta
1501 tgaaaacctg ctgaaagcgt cctttgagag gatggatcac ctgcgacagc tgcagaacat
1561 cattcaggcc acgtccaggg agatcatgtg gatcaatgac tgcgaggagg aggagctgct
1621 gtacgactgg agcgacaaga acaccaacat cgctcagaaa caggaggcct tctccatacg
1681 catgagtcaa ctggaagtta aagaaaaaga gctcaataag ctgaaacaag aaagtgacca
1741 acttgtcctc aatcagcatc cagcttcaga caaaattgag gcctatatgg acactctgca
1801 gacgcagtgg agttggattc ttcagatcac caagtgcatt gatgttcatc tgaaagaaaa
1861 tgctgcctac tttcagtttt ttgaagaggc gcagtctact gaagcatacc tgaaggggct
1921 ccaggactcc atcaggaaga agtacccctg cgacaagaac atgcccctgc agcacctgct
1981 ggaacagatc aaggagctgg agaaagaacg agagaaaatc cttgaataca agcgtcaggt
2041 gcagaacttg gtaaacaagt ctaagaagat tgtacagctg aagcctcgta acccagacta
2101 cagaagcaat aaacccatta ttctcagagc tctctgtgac tacaaacaag atcagaaaat
2161 cgtgcataag ggggatgagt gtatcctgaa ggacaacaac gagcgcagca agtggtacgt
2221 gacgggcccg ggaggcgttg acatgcttgt tccctctgtg gggctgatca tccctcctcc
2281 gaacccactg gccgtggacc tctcttgcaa gattgagcag tactacgaag ccatcttggc
2341 tctgtggaac cagctctaca tcaacatgaa gagcctggtg tcctggcact actgcatgat
2401 tgacatagag aagatcaggg ccatgacaat cgccaagctg aaaacaatgc ggcaggaaga
2461 ttacatgaag acgatagccg accttgagtt acattaccaa gagttcatca gaaatagcca
2521 aggctcagag atgtttggag atgatgacaa gcggaaaata cagtctcagt tcaccgatgc
2581 ccagaagcat taccagaccc tggtcattca gctccctggc tatccccagc accagacagt
2641 gaccacaact gaaatcactc atcatggaac ctgccaagat gtcaaccata ataaagtaat
2701 tgaaaccaac agagaaaatg acaagcaaga aacatggatg ctgatggagc tgcagaagat
2761 tcgcaggcag atagagcact gcgagggcag gatgactctc aaaaacctcc ctctagcaga
2821 ccaggggtct tctcaccaca tcacagtgaa aattaacgag cttaagagtg tgcagaatga
2881 ttcacaagca attgctgagg ttctcaacca gcttaaagat atgcttgcca acttcagagg
2941 ttctgaaaag tactgctatt tacagaatga agtatttgga ctatttcaga aactggaaaa
3001 tatcaatggt gttacagatg gctacttaaa tagcttatgc acagtaaggg cactgctcca
3061 ggctattctc caaacagaag acatgttaaa ggtttatgaa gccaggctca ctgaggagga
3121 aactgtctgc ctggacctgg ataaagtgga agcttaccgc tgtggactga agaaaataaa
3181 aaatgacttg aacttgaaga agtcgttgtt ggccactatg aagacagaac tacagaaagc
3241 ccagcagatc cactctcaga cttcacagca gtatccactt tatgatctgg acttgggcaa
3301 gttcggtgaa aaagtcacac agctgacaga ccgctggcaa aggatagata aacagatcga
3361 ctttagatta tgggacctgg agaaacaaat caagcaattg aggaattatc gtgataacta
3421 tcaggctttc tgcaagtggc tctatgatgc taaacgccgc caggattcct tagaatccat
3481 gaaatttgga gattccaaca cagtcatgcg gtttttgaat gagcagaaga acttgcacag
3541 tgaaatatct ggcaaacgag acaaatcaga ggaagtacaa aaaattgctg aactttgcgc
3601 caattcaatt aaggattatg agctccagct ggcctcatac acctcaggac tggaaactct
3661 gctgaacata cctatcaaga ggaccatgat tcagtcccct tctggggtga ttctgcaaga
3721 ggtaagtatc aaggttacaa gacaggttta aggagaccaa taagctatta ttcgtatagc
3781 atacattata cgaagttatt aagttatgga gaccggtgtg gcacgtgcgg accgagcggc
3841 cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg
3901 aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg
3961 agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt
4021 gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt
4081 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccttagc
4141 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
4201 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
4261 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
4321 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
4381 aacactcaac tctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggt
4441 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
4501 aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag
4561 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
4621 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
4681 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
4741 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
4801 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
4861 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
4921 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
4981 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
5041 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
5101 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
5161 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
5221 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
5281 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
5341 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
5401 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
5461 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
5521 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
5581 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
5641 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
5701 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
5761 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
5821 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
5881 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
5941 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
6001 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
6061 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
6121 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
6181 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
6241 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
6301 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
6361 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
6421 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
6481 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
6541 tttacggttc ctggcctttt gctggccttt tgctcacatg t
//
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