| 质粒名称: | pAAVLINK-EF1alpha-HA-5'-ARHGAP32 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001928 |
| 产品用途: | 5' AAVLINK plasmid for ARHGAP32 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1alpha |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | ARHGAP32 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1alpha-HA-5'-ARHGAP32
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1alpha-HA-5'-ARHGAP32载体质粒基本信息
pAAVLINK-EF1alpha-HA-5'-ARHGAP32质粒图谱载体图谱和pAAVLINK-EF1alpha-HA-5'-ARHGAP32载体序列质粒序列多克隆位点信息
pAAVLINK-EF1alpha-HA-5'-ARHGAP32质粒载体简介
pAAVLINK-EF1alpha-HA-5'-ARHGAP32质粒序列载体序列
LOCUS pAAVLINK-EF1alpha-HA-5'-ARHGAP32 7095 bp DNA circular SYN 10-AUG-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7095)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 16, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7095
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
/note="color: #ffe4c4"
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..4235
/codon_start=1
/gene="ARHGAP32"
/gene_synonym="GC-GAP; GRIT; p200RhoGAP; p250GAP;
PX-RICS;RICS"
/product="rho GTPase-activating protein 32 isoform 3"
/label=ARHGAP32
/note="isoform 3 is encoded by transcript variant 3;RhoGAP
involved in the -catenin-N-cadherin and NMDA receptor
signaling; brain-specific Rho GTP-ase-activating protein;
rho/Cdc42/Rac GTPase-activating protein RICS; rac GTPase
activating protein; GAB-associated CDC42; rho
GTPase-activating protein 32; GTPase regulator interacting
with TrkA; brain-specific Rho GTPase-activating protein;
GAB-associated Cdc42/Rac GTPase-activating protein; rhoGAP
involved in the beta-catenin-N-cadherin and NMDA receptor
signaling; rho-type GTPase-activating protein 32;
GTPase-activating protein for Cdc42 and Rac1"
/db_xref="CCDS:CCDS91623.1"
/db_xref="GeneID:9743"
/db_xref="HGNC:HGNC:17399"
/db_xref="MIM:608541"
/protein_id="NP_001364953.1"
/translation="METESESSTLGDDSVFWLESEVIIQVTDCEEEEREEKFRKMKSSV
HSEEDDFVPELHRNVHPRERPDWEETLSAMARGADVPEIPGDLTLKTCGSTASMKVKHV
KKSTTPGLMGCDNIHRLPFTKGHFPKMAECAHFHYENVEFGSIQLSLSEEQNEVMKNGC
ESKELVYLVQIACQGKSWIVKRSYEDFRVLDKHLHLCIYDRRFSQLSELPRSDTLKDSP
ESVTQMLMAYLSRLSAIAGNKINCGPALTWMEIDNKGNHLLVHEESSINTPAVGAAHVI
KRYTARAPDELTLEVGDIVSVIDMPPKVLSTWWRGKHGFQVGLFPGHCVELINQKVPQS
VTNSVPKPVSKKHGKLITFLRTFMKSRPTKQKLKQRGILKERVFGCDLGEHLLNSGFEV
PQVLQSCTAFIERYGIVDGIYRLSGVASNIQRLRHEFDSEHVPDLTKEPYVQDIHSVGS
LCKLYFRELPNPLLTYQLYEKFSDAVSAATDEERLIKIHDVIQQLPPPHYRTLEFLMRH
LSLLADYCSITNMHAKNLAIVWAPNLLRSKQIESACFSGTAAFMEVRIQSVVVEFILNH
VDVLFSGRISMAMQEGAASLSRPKSLLVSSPSTKLLTLEEAQARTQAQVNSPIVTENKY
IEVGEGPAALQGKFHTIIEFPLERKRPQNKMKKSPVGSWRSFFNLGKSSSVSKRKLQRN
ESEPSEMKAMALKGGRAEGTLRSAKSEESLTSLHAVDGDSKLFRPRRPRSSSDALSASF
NGEMLGNRCNSYDNLPHDNESEEEGGLLHIPALMSPHSAEDVDLSPPDIGVASLDFDPM
SFQCSPPKAESECLESGASFLDSPGYSKDKPSANKKDAETGSSQCQTPGSTASSEPVSP
LQEKLSPFFTLDLSPTEDKSSKPSSFTEKVVYAFSPKIGRKLSKSPSMSISEPISVTLP
PRVSEVIGTVSNTTAQNASSSTWDKCVEERDATNRSPTQIVKMKTNETVAQEAYESEVQ
PLDQVAAEEVELPGKEDQSVSSSQSKAVASGQTQTGAVTHDPPQDSVPVSSVSLIPPPP
PPKNVARMLALALAESAQQASTQSLKRPGTSQAGYTNYGDIAVATTEDNLSSSYSAVAL
DKAYFQTDRPAEQFHLQNNAPGNCDHPLPETTATGDPTHSNTTESGEQHHQVDLT"
misc_feature 2551..2556
/label=SDS
misc_feature 3072..3077
/label=SDS
misc_feature 3079..3084
/label=SDS
misc_feature 3200..3205
/label=SDS
misc_feature 3432..3437
/label=SDS
intron 4236..4276
/label=Splicing Donor
misc_feature 4280..4313
/label=lox2272-JT1571
repeat_region 4358..4498
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4358..4487
/label=AAV2 ITR
repeat_region 4359..4499
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 4573..5028
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5310..5414
/gene="bla"
/label=AmpR promoter
CDS 5415..6275
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6446..7034
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctat ggagactgaa agtgagagta gcactttagg
781 ggatgacagt gtcttctggt tggagtctga agttataatc caggtgactg actgtgaaga
841 ggaagaaagg gaagagaagt tcaggaagat gaagtcctca gtacattctg aagaagatga
901 ttttgttcca gagctacata gaaatgtaca ccctcgagag cggcctgatt gggaagaaac
961 tcttagcgca atggcaagag gcgcagatgt tccagagatt cctggagatc ttactcttaa
1021 gacgtgtggc agtacagcca gtatgaaggt taagcatgtg aaaaagtcca ccactccagg
1081 actgatgggc tgtgacaaca ttcacaggtt acccttcact aaaggtcact ttccgaagat
1141 ggctgaatgt gcgcatttcc attatgagaa tgttgagttc ggcagcatac agctttcact
1201 ttcagaagaa cagaatgaag taatgaaaaa tggctgtgaa tctaaagagc tggtctacct
1261 cgtgcagatt gcttgtcagg gaaaaagttg gattgttaaa agaagttatg aagattttcg
1321 ggtacttgat aaacatcttc atctgtgtat ttatgaccga agattttccc agctctcaga
1381 acttccccgt tctgacaccc tgaaggacag tccagagtcg gtcactcaga tgcttatggc
1441 ttacctgtca cgcctttcag ctatcgctgg caacaagatc aactgtgggc ccgcccttac
1501 ctggatggag attgataata agggaaatca ccttttggtt catgaggagt catccatcaa
1561 cactcctgct gtcggtgctg cccatgttat caagaggtac actgctcggg cccctgacga
1621 actgacctta gaggtgggag acattgtttc tgttattgac atgcccccga aagtgttaag
1681 cacatggtgg agaggcaagc acggattcca ggtgggactc ttccctggac actgtgttga
1741 gttaattaac caaaaagttc cccagtcagt gaccaactca gtgccaaaac cagtgtctaa
1801 aaagcacggc aagctcatta cgttcttacg aacattcatg aagtctcgtc caacaaaaca
1861 gaagctgaag cagcggggaa tcttgaaaga gagggtgttt ggttgtgacc tgggggaaca
1921 ccttctaaat tctggttttg aagtgccgca ggttcttcaa agctgcacag cattcattga
1981 gagatatggc atcgtggatg gaatctatcg cctttctggt gttgcctcca atatccagag
2041 actacgccat gaatttgact ctgagcacgt ccccgacctg acgaaagaac cgtatgttca
2101 ggacatccat tctgtgggtt ccctatgtaa gctgtacttc cgggaactcc caaaccctct
2161 gcttacctac cagctgtatg agaaattttc tgatgcagtt tcagcagcaa cagatgaaga
2221 aaggctgata aaaatccacg atgtcatcca gcagctcccc ccaccacact acagaacact
2281 ggagttcctg atgagacact tgtctcttct agctgactat tgttccatca caaatatgca
2341 tgcaaaaaat ctagcaattg tttgggctcc aaacctgtta agatcaaaac agatagaatc
2401 tgcctgcttc agtggaacag cagctttcat ggaagtgagg attcagtctg tggttgttga
2461 gttcatcctg aatcacgttg atgtgctgtt cagcggcaga atcagcatgg ccatgcaaga
2521 gggggcagct tctctatcaa ggcccaagtc cctcctggta tcctctccat ccaccaaact
2581 gctgacattg gaagaggccc aggcacgaac acaagctcag gtcaattctc caattgtgac
2641 ggaaaataaa tatatcgaag taggagaagg acctgctgca cttcagggga aatttcatac
2701 cataattgag ttcccacttg aaagaaagag gcctcaaaat aagatgaaaa agtctcctgt
2761 gggtagctgg cgttcctttt tcaacttggg gaaatcatca tctgtttcta aacgaaagct
2821 gcagcggaat gagagtgagc cttcagagat gaaagccatg gctctgaaag gtggcagggc
2881 agaaggaacc ctccgttcag ctaaaagtga ggagtctctt acatctctcc atgcagttga
2941 tggtgattct aagctcttcc gacccagaag acccagatct tccagtgatg cactgtctgc
3001 ctcttttaat ggagaaatgc tggggaaccg ctgtaactcc tatgataatc tgcctcatga
3061 caatgagagt gaggaggaag gagggctgct tcatatccca gcccttatgt ctcctcattc
3121 agctgaggat gttgacttga gcccaccaga cattggagta gccagcctgg attttgatcc
3181 aatgtcattt caatgtagtc ctcctaaggc cgaatcagaa tgtctggaga gtggtgcttc
3241 ctttttagat tcaccaggat actccaagga taaaccaagt gccaataaaa aggatgcaga
3301 aacaggtagt agccaatgtc agactccagg aagcacagca agctctgaac ctgtctctcc
3361 tcttcaggag aaactgagtc cattctttac cctggacttg agcccaactg aagataaatc
3421 atctaagcca tcctccttta ctgaaaaggt cgtctatgct ttctctccga agataggacg
3481 gaaattaagc aaatcacctt ctatgagcat atctgagcca atttcagtga ccctaccacc
3541 acgggtgtca gaagtcattg gtacagtctc aaataccaca gctcagaatg catcatcttc
3601 aacctgggac aaatgcgttg aagaaaggga tgccacaaat agatccccca cccagatagt
3661 aaagatgaaa acaaatgaga cagttgccca agaagcatat gaatctgaag tccagcccct
3721 ggaccaggtg gctgctgaag aagtagaatt gccagggaaa gaggatcagt ctgtctcaag
3781 cagtcagagt aaggctgtag cttctggaca gactcagaca ggagcagtta cccatgaccc
3841 ccctcaggat tccgttcctg tcagttcagt ctctcttatc ccaccaccac cgcctccgaa
3901 aaatgttgcc cgaatgttgg cgctagcatt agctgagtcc gcacagcaag cctcaactca
3961 gtcattgaag agaccaggga cctctcaggc tgggtataca aattatggag acatagcggt
4021 ggctacaact gaagataatc tgtccagttc ttactctgca gttgctctag ataaggccta
4081 tttccaaacc gatcgaccag cagagcagtt ccacctccag aataatgcac caggaaactg
4141 tgaccatcct ctaccagaga caacagctac tggggatcct acccattcca acacaactga
4201 atctggggag caacatcacc aagtagactt aacaggtaag tatcaaggtt acaagacagg
4261 tttaaggaga ccaataagct attattcgta taggatactt tatacgaagt tattaagtta
4321 tggagaccgg tgtggcacgt gcggaccgag cggccgcagg aacccctagt gatggagttg
4381 gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga
4441 cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg
4501 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa
4561 agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
4621 gcagcgtgac cgctacactt gccagcgcct tagcgcccgc tcctttcgct ttcttccctt
4681 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
4741 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt
4801 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
4861 tctttaatag tggactcttg ttccaaactg gaacaacact caactctatc tcgggctatt
4921 cttttgattt ataagggatt ttgccgattt cggtctattg gttaaaaaat gagctgattt
4981 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc
5041 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
5101 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
5161 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
5221 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
5281 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
5341 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
5401 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
5461 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
5521 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
5581 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
5641 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
5701 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
5761 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
5821 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
5881 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
5941 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
6001 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
6061 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
6121 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
6181 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
6241 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
6301 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
6361 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
6421 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
6481 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
6541 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
6601 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
6661 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
6721 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
6781 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
6841 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
6901 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
6961 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
7021 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
7081 cttttgctca catgt
//
pAAVLINK-EF1alpha-HA-5'-ARHGAP32载体图谱质粒图谱pdf版和pAAVLINK-EF1alpha-HA-5'-ARHGAP32载体序列质粒序列等相关资料下载
pAAVLINK-EF1alpha-HA-5'-ARHGAP32质粒载体应用举例
- 上一篇：pAAVLINK-3'-NSD1-FLAG-bGHpA
- 下一篇：没有了
