| 质粒名称: | pET-28a-UBA1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001345 |
| 产品用途: | Express proteins in vitro |
| 存储实验室: | Peiguo YANG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pET-28a |
| 表达系统: | 细菌表达 |
| 启动子: | T7, Lac |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 卡那霉素氯霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | UBA1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Coiled-coil-domain-mediated TRIM E3 protein condensation modulates enzymatic activity and functional specificity
Qi Chen, Peiguo Yang
Cell Reports, 115766, June 24, 2025
|
买家导航
pET-28a-UBA1载体质粒基本信息
pET-28a-UBA1质粒图谱载体图谱和pET-28a-UBA1载体序列质粒序列多克隆位点信息
pET-28a-UBA1质粒载体简介
pET-28a-UBA1质粒序列载体序列
LOCUS pET-28a-UBA1 8413 bp DNA circular SYN 29-AUG-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8413)
AUTHORS 15129015045
TITLE Direct Submission
JOURNAL Exported Apr 18, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8413
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin 12..467
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
CDS complement(560..1375)
/codon_start=1
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
rep_origin 1497..2085
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 2271..2413
/label=bom
/note="basis of mobility region from pBR322"
CDS complement(2515..2706)
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/label=rop
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
CDS complement(3515..4597)
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
promoter complement(4598..4675)
/gene="lacI"
/label=lacI promoter
promoter 4984..5002
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 5003..5027
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 5049..5070
/label=Primer 1
primer_bind 5055..5094
/label=28a-UBA1-F
misc_feature 5077..8250
/label=UBA1
primer_bind 5932..5950
/label=UBA1-seqF0
primer_bind 6657..6674
/label=UBA1-seqF1
primer_bind complement(8233..8269)
/label=28a-UBA1-R
primer_bind complement(8251..8269)
/label=Primer 2
CDS 8257..8274
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
terminator 8341..8388
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
ORIGIN
1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt
481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
541 tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat
601 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
661 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
721 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
781 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc
841 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac
901 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac
961 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat
1021 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag
1081 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca
1141 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac
1201 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg
1261 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca
1321 tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac
1381 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa
1441 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
1501 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
1561 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
1621 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
1681 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
1741 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
1801 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
1861 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
1921 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
1981 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
2041 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
2101 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
2161 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
2221 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
2281 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta
2341 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
2401 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
2461 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
2521 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc
2581 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag
2641 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt
2701 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa
2761 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg
2821 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg
2881 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc
2941 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta
3001 cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca
3061 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc
3121 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc
3181 catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa
3241 ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc
3301 gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac
3361 gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca
3421 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta
3481 atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
3541 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
3601 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca
3661 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa
3721 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt
3781 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg
3841 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca
3901 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta
3961 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg
4021 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat
4081 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct
4141 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg
4201 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat
4261 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc
4321 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca
4381 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg
4441 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt
4501 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg
4561 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct
4621 cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga
4681 tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg
4741 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc
4801 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg
4861 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg
4921 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga
4981 aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa
5041 ttttgtttaa ctttaagaag gagatatacc gaattcatgt ccagctcgcc gctgtccaag
5101 aaacgtcgcg tgtccgggcc tgatccaaag ccgggttcta actgctcccc tgcccagtcc
5161 gtgttgtccg aagtgccctc ggtgccaacc aacggaatgg ccaagaacgg cagtgaagca
5221 gacatagacg agggccttta ctcccggcag ctgtatgtgt tgggccatga ggcaatgaag
5281 cggctccaga catccagtgt cctggtatca ggcctgcggg gcctgggcgt ggagatcgct
5341 aagaacatca tccttggtgg ggtcaaggct gttaccctac atgaccaggg cactgcccag
5401 tgggctgatc tttcctccca gttctacctg cgggaggagg acatcggtaa aaaccgggcc
5461 gaggtatcac agccccgcct cgctgagctc aacagctatg tgcctgtcac tgcctacact
5521 ggacccctcg ttgaggactt ccttagtggt ttccaggtgg tggtgctcac caacaccccc
5581 ctggaggacc agctgcgagt gggtgagttc tgtcacaacc gtggcatcaa gctggtggtg
5641 gcagacacgc ggggcctgtt tgggcagctc ttctgtgact ttggagagga aatgatcctc
5701 acagattcca atggggagca gccactcagt gctatggttt ctatggttac caaggacaac
5761 cccggtgtgg ttacctgcct ggatgaggcc cgacacgggt ttgagagcgg ggactttgtc
5821 tccttttcag aagtacaggg catggttgaa ctcaacggaa atcagcccat ggagatcaaa
5881 gtcctgggtc cttatacctt tagcatctgt gacacctcca acttctccga ctacatccgt
5941 ggaggcatcg tcagtcaggt caaagtacct aagaagatta gctttaaatc cttggtggcc
6001 tcactggcag aacctgactt tgtggtgacg gacttcgcca agttttctcg ccctgcccag
6061 ctgcacattg gcttccaggc cctgcaccag ttctgtgctc agcatggccg gccacctcgg
6121 ccccgcaatg aggaggatgc agcagaactg gtagccttag cacaggctgt gaatgctcga
6181 gccctgccag cagtgcagca aaataacctg gacgaggacc tcatccggaa gctggcatat
6241 gtggctgctg gggatctggc acccataaac gccttcattg ggggcctggc tgcccaggaa
6301 gtcatgaagg cctgctccgg gaagttcatg cccatcatgc agtggctata ctttgatgcc
6361 cttgagtgtc tccctgagga caaagaggtc ctcacagagg acaagtgcct ccagcgccag
6421 aaccgttatg acgggcaagt ggctgtgttt ggctcagacc tgcaagagaa gctgggcaag
6481 cagaagtatt tcctggtggg tgcgggggcc attggctgtg agctgctcaa gaactttgcc
6541 atgattgggc tgggctgcgg ggagggtgga gaaatcatcg ttacagacat ggacaccatt
6601 gagaagtcaa atctgaatcg acagtttctt ttccggccct gggatgtcac gaagttaaag
6661 tctgacacgg ctgctgcagc tgtgcgccaa atgaatccac atatccgggt gacaagccac
6721 cagaaccgtg tgggtcctga cacggagcgc atctatgatg acgatttttt ccaaaaccta
6781 gatggcgtgg ccaatgccct ggacaacgtg gatgcccgca tgtacatgga ccgccgctgt
6841 gtctactacc ggaagccact gctggagtca ggcacactgg gcaccaaagg caatgtgcag
6901 gtggtgatcc ccttcctgac agagtcgtac agttccagcc aggacccacc tgagaagtcc
6961 atccccatct gtaccctgaa gaacttccct aatgccatcg agcacaccct gcagtgggct
7021 cgggatgagt ttgaaggcct cttcaagcag ccagcagaaa atgtcaacca gtacctcaca
7081 gaccccaagt ttgtggagcg aacactgcgg ctggcaggca ctcagccctt ggaggtgctg
7141 gaggctgtgc agcgcagcct ggtgctgcag cgaccacaga cctgggctga ctgcgtgacc
7201 tgggcctgcc accactggca cacccagtac tcgaacaaca tccggcagct gctgcacaac
7261 ttccctcctg accagctcac aagctcagga gcgccgttct ggtctgggcc caaacgctgt
7321 ccacacccgc tcacctttga tgtcaacaat cccctgcatc tggactatgt gatggctgct
7381 gccaacctgt ttgcccagac ctacgggctg acaggctctc aggaccgagc tgctgtggcc
7441 acattcctgc agtctgtgca ggtccccgaa ttcaccccca agtctggcgt caagatccat
7501 gtttctgacc aggagctgca gagcgccaat gcctctgttg atgacagtcg tctagaggag
7561 ctcaaagcca ctctgcccag cccagacaag ctccctggat tcaagatgta ccccattgac
7621 tttgagaagg atgatgacag caactttcat atggatttca tcgtggctgc atccaacctc
7681 cgggcagaaa actatgacat tccttctgca gaccggcaca agagcaagct gattgcaggg
7741 aagatcatcc cagccattgc cacgaccaca gcagccgtgg ttggccttgt gtgtctggag
7801 ctgtacaagg ttgtgcaggg gcaccgacag cttgactcct acaagaatgg tttcctcaac
7861 ttggccctgc ctttctttgg tttctctgaa ccccttgccg caccacgtca ccagtactat
7921 aaccaagagt ggacattgtg ggatcgcttt gaggtacaag ggctgcagcc taatggtgag
7981 gagatgaccc tcaaacagtt cctcgactat tttaagacag agcacaaatt agagatcacc
8041 atgctgtccc agggcgtgtc catgctctat tccttcttca tgccagctgc caagctcaag
8101 gaacggttgg atcagccgat gacagagatt gtgagccgtg tgtcgaagcg aaagctgggc
8161 cgccacgtgc gggcgctggt gcttgagctg tgctgtaacg acgagagcgg cgaggatgtc
8221 gaggttccct atgtccgata caccatccgc ctcgagcacc accaccacca ccactgagat
8281 ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa
8341 ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga
8401 actatatccg gat
//
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