pAAV-hSyn-Asp-RGEPO1

LOCUS       pAAV-hSyn-Asp-RGEPO1               5911 bp DNA     circular SYN 22-MAR-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5911)
  AUTHORS   Kiryl Piatkevich
  TITLE     Direct Submission
  JOURNAL   Exported Jul 23, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..5911
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    311..800
                     /label=Syn
     promoter        349..796
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     primer_bind     complement(701..710)
                     /label=PD-R1
     primer_bind     734..755
                     /label=Syn-F
     primer_bind     787..836
                     /label=Asp-syn-F1
                     /note="C08"
     misc_feature    812..817
                     /label=Kozak sequence
     CDS             818..889
                     /codon_start=1
                     /label=Asp-tag
                     /translation="MASSLYSFLLALSIVYIFVAPTHS"
     CDS             890..916
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     912..956
                     /label=PD-F1
     CDS             938..1390
                     /codon_start=1
                     /label=N-mApple
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTARLKVTKGGPLPFAWDILSPQFMYGSKAYIKHPADIPDYFKLSFPEGFRWERVMSF
                     EDGGIIHVNQDSSLQDGVFIYKVKLLGTNFPPDGPVMQKKTMGWEAS"
     primer_bind     1364..1411
                     /label=FR-1-3-F
     primer_bind     1364..1385
                     /label=FR-Y-Ra-F
     primer_bind     complement(1373..1393)
                     /label=mApple-N-R1
     primer_bind     complement(1377..1390)
                     /label=N-mapple-R
     primer_bind     complement(1379..1397)
                     /label=FR-1-3-R
     CDS             1391..1393
                     /codon_start=1
                     /label=LINKER1
                     /translation="P"
     primer_bind     complement(1392..1441)
                     /label=F6A-R1
     CDS             1394..1825
                     /codon_start=1
                     /label=YgaU_Hydrothermal
                     /translation="GIWDFVKSAGRKIGIGSEEAAPAADDLKKELDSYKLGTDDVKVEV
                     VGDKAVITGNVADQSILEKAIVAVGNTLGISKVESNVEVPDAKEPVFHTVKKGDTLWAI
                     ASKQYGNVSKYNAIFEANKPMLSHPDKIYPGQVLRIPDLG"
     primer_bind     complement(1397..1441)
                     /label=V7A-R1
     primer_bind     complement(1400..1441)
                     /label=K8A-R1
     primer_bind     complement(1404..1441)
                     /label=S9A-R1
     misc_feature    1406..1426
                     /label=Mutant
     primer_bind     complement(1411..1441)
                     /label=G11A-R1
     primer_bind     complement(1411..1441)
                     /label=R12A-R1
     primer_bind     1427..1444
                     /label=0811-F2
     primer_bind     complement(1588..1639)
                     /label=G71A-R1
     primer_bind     complement(1589..1639)
                     /label=N72A-R1
     primer_bind     complement(1592..1639)
                     /label=T73A-R1
     misc_feature    1601..1624
                     /label=Mmutant
     primer_bind     complement(1603..1639)
                     /label=G75A-R1
     primer_bind     complement(1604..1639)
                     /label=I76A-R1
     primer_bind     complement(1606..1639)
                     /label=S77A-R1
     primer_bind     complement(1611..1640)
                     /label=K78A-R1
     primer_bind     complement(1613..1639)
                     /label=L74A-R1
     primer_bind     1625..1643
                     /label=0811-F1
     primer_bind     complement(1717..1741)
                     /label=G113V-R
     primer_bind     complement(1802..1818)
                     /label=Ec-kbp-R
     primer_bind     1826..1844
                     /label=mApple-C-F1
     CDS             1826..1834
                     /codon_start=1
                     /label=LINKER
                     /translation="SAC"
     CDS             1835..2092
                     /codon_start=1
                     /label=C-mApple
                     /translation="VSEWMYPEDGALKSEIRKGLRLKDGGHYAAEVKTTYKAKKPVQLP
                     GAYIVDIELDILSHNEDYTIVEQCERAVGRHPTGGTDALYK"
     primer_bind     1835..1855
                     /label=C-mApple-F2
     primer_bind     complement(2025..2044)
                     /label=FR-R
     primer_bind     complement(2074..2113)
                     /label=PD-R1
     primer_bind     2093..2113
                     /label=MYC-F1
     CDS             2102..2131
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2135..2281
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     primer_bind     complement(2263..2285)
                     /label=MYC-R1
     primer_bind     complement(2264..2307)
                     /label=Asp-syn-R1
     primer_bind     complement(2281..2305)
                     /label=0611-GCaMP8f-R
     misc_feature    2309..2897
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(2780..2791)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2915..3122
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   3174..3314
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      3389..3844
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4126..4230
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4231..5091
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5262..5850
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttgcaaa gatggataaa gttttaaaca gagaggaatc
      181 tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc
      241 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcagca
      301 aattaattaa tggttaatta atctagactg cagagggccc tgcgtatgag tgcaagtggg
      361 ttttaggacc aggatgaggc ggggtggggg tgcctacctg acgaccgacc ccgacccact
      421 ggacaagcac ccaaccccca ttccccaaat tgcgcatccc ctatcagaga gggggagggg
      481 aaacaggatg cggcgaggcg cgtgcgcact gccagcttca gcaccgcgga cagtgccttc
      541 gcccccgcct ggcggcgcgc gccaccgccg cctcagcact gaaggcgcgc tgacgtcact
      601 cgccggtccc ccgcaaactc cccttcccgg ccaccttggt cgcgtccgcg ccgccgccgg
      661 cccagccgga ccgcaccacg cgaggcgcga gatagggggg cacgggcgcg accatctgcg
      721 ctgcggcgcc ggcgactcag cgctgcctca gtctgcggtg ggcagcggag gagtcgtgtc
      781 gtgcctgaga gcgcagtcga gaggatccta agccaccatg gccagcagcc tgtacagctt
      841 cctgctggcc ctgagcatcg tgtacatctt cgtggccccc acccacagct atccatatga
      901 tgttccagat tatgctgggg cccagccggc cagatctatg gtgagcaagg gcgaggagga
      961 taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa
     1021 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg ccttccagac
     1081 cgctaggctg aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc
     1141 tcagttcatg tacggctcca aggcctacat taagcaccca gccgacatcc ccgactactt
     1201 caagctgtcc ttccccgagg gcttcaggtg ggagcgcgtg atgagcttcg aggacggcgg
     1261 cattattcac gttaatcagg actcctccct gcaggacggc gtattcatct acaaggtgaa
     1321 gctgctcggc accaacttcc cccccgacgg ccccgtaatg cagaagaaga ccatgggctg
     1381 ggaggcttcc cccggcatct gggacttcgt gaagagcgcc ggcaggaaga tcggcatcgg
     1441 cagcgaggag gccgcccccg ccgccgacga cctgaagaag gagctggaca gctacaagct
     1501 gggcaccgac gacgtgaagg tggaggtggt gggcgacaag gccgtgatca ccggcaacgt
     1561 ggccgaccag agcatcctgg agaaggccat cgtggccgtg ggcaacaccc tgggcatcag
     1621 caaggtggag agcaacgtgg aggtgcccga cgccaaggag cccgtgttcc acaccgtgaa
     1681 gaagggcgac accctgtggg ccatcgccag caagcagtac ggcaacgtca gcaagtacaa
     1741 cgccatcttc gaggccaaca agcccatgct gagccacccc gacaagatct accccggcca
     1801 ggtgctgagg atccccgacc tgggctctgc ctgcgtttcc gagtggatgt accccgagga
     1861 cggcgccctg aagagcgaga tcaggaaggg gctgaggctg aaggacggcg gccactacgc
     1921 cgccgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacat
     1981 cgtcgacatc gagttggaca tcttgtccca caacgaggac tacaccatcg tggaacagtg
     2041 cgaacgcgcc gtgggccgcc accccaccgg tggcacggac gcgctgtaca agggcgtcga
     2101 cgaacaaaaa ctcatctcag aagaggatct gaatgctgtg ggccaggaca cgcaggaggt
     2161 catcgtggtg ccacactcct tgccctttaa ggtggtggtg atctcagcca tcctggccct
     2221 ggtggtgctc accatcatct cccttatcat cctcatcatg ctttggcaga agaagccacg
     2281 ttaggaattc cgtacaaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag
     2341 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     2401 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     2461 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     2521 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     2581 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     2641 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     2701 gaaatcatcg tcctttcctt ggctgctcgc ctatgttgcc acctggattc tgcgcgggac
     2761 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     2821 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     2881 ttgggccgcc tccccgcatc gataccggct cgagctgtgc cttctagttg ccagccatct
     2941 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     3001 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     3061 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     3121 gatgcggtct cgagctgatt ttgtaggtaa ccacgtgcgg accgagcggc cgcaggaacc
     3181 cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg
     3241 accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg
     3301 cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     3361 acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg
     3421 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct
     3481 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     3541 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     3601 gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     3661 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     3721 tctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggt ctattggtta
     3781 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca
     3841 attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     3901 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     3961 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     4021 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     4081 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     4141 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     4201 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     4261 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     4321 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     4381 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     4441 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     4501 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     4561 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     4621 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     4681 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     4741 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     4801 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     4861 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     4921 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     4981 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     5041 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     5101 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     5161 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     5221 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     5281 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     5341 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     5401 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     5461 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     5521 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     5581 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     5641 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     5701 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     5761 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     5821 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     5881 ctggcctttt gctggccttt tgctcacatg t
//