| 质粒名称: | pTE4396 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 74041 |
| 存储实验室: | Ervin Welker |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 9677bp |
| 完整载体大小： | 4989 bp |
| 载体修饰: | 人细胞色素c氧化酶亚基VIII（human cytochrome C oxidase subunit VIII） |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | pcDNA3.1 |
| 物种来源: | -- |
| 插入大小: | 47bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | 人细胞色素c氧化酶亚基VIII（N端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | AgeI(未破坏) |
| 3' 克隆位点: | NotI (未破坏) |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | pTE4396 was a gift from Ervin Welker (Addgene plasmid # 74041 ; http://n2t.net/addgene:74041 ; RRID:Addgene_74041) |
| 参考文献部分: | Cpf1 nucleases demonstrate robust activity to induce DNA modification by exploiting homology directed repair pathways in mammalian cells. Toth E, Weinhardt N, Bencsura P, Huszar K, Kulcsar PI, Talas A, Fodor E, Welker E. Biol Direct. 2016 Sep 14;11:46. doi: 10.1186/s13062-016-0147-0. 10.1186/s13062-016-0147-0 [pii] PubMed 27630115 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pTE4396 9680 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Expresses human codon-optimized AsCpf1 and As crRNA..
ACCESSION .
VERSION .
KEYWORDS pTE4396
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9680)
AUTHORS Toth E, Weinhardt N, Bencsura P, Huszar K, Kulcsar PI, Talas A,
Fodor E, Welker E
TITLE Cpf1 nucleases demonstrate robust activity to induce DNA
modification by exploiting homology directed repair pathways in
mammalian cells.
JOURNAL Biol Direct. 2016 Sep 14;11:46. doi: 10.1186/s13062-016-0147-0.
PUBMED 27630115
REFERENCE 2 (bases 1 to 9680)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9680
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
promoter complement(221..461)
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
primer_bind complement(271..290)
/label=LKO.1 5'
/note="Human U6 promoter, forward primer"
primer_bind complement(441..461)
/label=hU6-F
/note="Human U6 promoter, forward primer"
enhancer 524..827
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 828..1031
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 982..1002
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 1076..1095
/label=T7
/note="T7 promoter, forward primer"
promoter 1076..1094
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 1142..5062
/codon_start=1
/product="CRISPR-associated protein from Acidaminococcus
sp. BV3L6 (Zetsche et al., 2015)"
/label=AsCpf1
/translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN
AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD
KFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF
ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL
NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL
RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG
KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL
KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA
RNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYK
ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP
LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID
LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK
DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQR
SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV
VVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ
FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD
VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN
HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM
RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES
KDLKLQNGISNQDWLAYIQELRN"
regulatory 4782..4791
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 5063..5110
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
CDS 5117..5197
/codon_start=1
/product="three tandem HA epitope tags"
/label=3xHA
/translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
primer_bind complement(5271..5288)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 5277..5501
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 5547..5975
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5634..5653)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5844..5865
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(5984..6004)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 5989..6318
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 6169..6304
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 6231..6250
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 6385..7179
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(6439..6458)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 7049..7068
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 7353..7474
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7390..7409)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7444..7463
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(7523..7539)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7523..7539)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7536..7558)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7547..7563
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7571..7601)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7616..7637
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7754..7771)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7925..8513)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8005..8024)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8684..9544)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9307..9326
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9545..9649)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattaaaaa aggagacggg
181 atcccgtctc catctacaag agtagaaatt acggtgtttc gtcctttcca caagatatat
241 aaagccaaga aatcgaaata ctttcaagtt acggtaagca tatgatagtc cattttaaaa
301 cataatttta aaactgcaaa ctacccaaga aattattact ttctacgtca cgtattttgt
361 actaatatct ttgtgtttac agtcaaatta attccaatta tctctctaac agccttgtat
421 cgtatatgca aatatgaagg aatcatggga aataggccct cactagttat taatagtaat
481 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
541 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
601 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
661 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
721 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
781 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
841 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
901 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
961 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
1021 taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata
1081 cgactcacta tagggagacc caagctggct agcgtttaaa cttaagcttg gtaccgccac
1141 catgacacag ttcgagggct ttaccaacct gtatcaggtg agcaagacac tgcggtttga
1201 gctgatccca cagggcaaga ccctgaagca catccaggag cagggcttca tcgaggagga
1261 caaggcccgc aatgatcact acaaggagct gaagcccatc atcgatcgga tctacaagac
1321 ctatgccgac cagtgcctgc agctggtgca gctggattgg gagaacctga gcgccgccat
1381 cgactcctat agaaaggaga aaaccgagga gacaaggaac gccctgatcg aggagcaggc
1441 cacatatcgc aatgccatcc acgactactt catcggccgg acagacaacc tgaccgatgc
1501 catcaataag agacacgccg agatctacaa gggcctgttc aaggccgagc tgtttaatgg
1561 caaggtgctg aagcagctgg gcaccgtgac cacaaccgag cacgagaacg ccctgctgcg
1621 gagcttcgac aagtttacaa cctacttctc cggcttttat gagaacagga agaacgtgtt
1681 cagcgccgag gatatcagca cagccatccc acaccgcatc gtgcaggaca acttccccaa
1741 gtttaaggag aattgtcaca tcttcacacg cctgatcacc gccgtgccca gcctgcggga
1801 gcactttgag aacgtgaaga aggccatcgg catcttcgtg agcacctcca tcgaggaggt
1861 gttttccttc cctttttata accagctgct gacacagacc cagatcgacc tgtataacca
1921 gctgctggga ggaatctctc gggaggcagg caccgagaag atcaagggcc tgaacgaggt
1981 gctgaatctg gccatccaga agaatgatga gacagcccac atcatcgcct ccctgccaca
2041 cagattcatc cccctgttta agcagatcct gtccgatagg aacaccctgt ctttcatcct
2101 ggaggagttt aagagcgacg aggaagtgat ccagtccttc tgcaagtaca agacactgct
2161 gagaaacgag aacgtgctgg agacagccga ggccctgttt aacgagctga acagcatcga
2221 cctgacacac atcttcatca gccacaagaa gctggagaca atcagcagcg ccctgtgcga
2281 ccactgggat acactgagga atgccctgta tgagcggaga atctccgagc tgacaggcaa
2341 gatcaccaag tctgccaagg agaaggtgca gcgcagcctg aagcacgagg atatcaacct
2401 gcaggagatc atctctgccg caggcaagga gctgagcgag gccttcaagc agaaaaccag
2461 cgagatcctg tcccacgcac acgccgccct ggatcagcca ctgcctacaa ccctgaagaa
2521 gcaggaggag aaggagatcc tgaagtctca gctggacagc ctgctgggcc tgtaccacct
2581 gctggactgg tttgccgtgg atgagtccaa cgaggtggac cccgagttct ctgcccggct
2641 gaccggcatc aagctggaga tggagccttc tctgagcttc tacaacaagg ccagaaatta
2701 tgccaccaag aagccctact ccgtggagaa gttcaagctg aactttcaga tgcctacact
2761 ggcctctggc tgggacgtga ataaggagaa gaacaatggc gccatcctgt ttgtgaagaa
2821 cggcctgtac tatctgggca tcatgccaaa gcagaagggc aggtataagg ccctgagctt
2881 cgagcccaca gagaaaacca gcgagggctt tgataagatg tactatgact acttccctga
2941 tgccgccaag atgatcccaa agtgcagcac ccagctgaag gccgtgacag cccactttca
3001 gacccacaca acccccatcc tgctgtccaa caatttcatc gagcctctgg agatcacaaa
3061 ggagatctac gacctgaaca atcctgagaa ggagccaaag aagtttcaga cagcctacgc
3121 caagaaaacc ggcgaccaga agggctacag agaggccctg tgcaagtgga tcgacttcac
3181 aagggatttt ctgtccaagt ataccaagac aacctctatc gatctgtcta gcctgcggcc
3241 atcctctcag tataaggacc tgggcgagta ctatgccgag ctgaatcccc tgctgtacca
3301 catcagcttc cagagaatcg ccgagaagga gatcatggat gccgtggaga caggcaagct
3361 gtacctgttc cagatctata acaaggactt tgccaagggc caccacggca agcctaatct
3421 gcacacactg tattggaccg gcctgttttc tccagagaac ctggccaaga caagcatcaa
3481 gctgaatggc caggccgagc tgttctaccg ccctaagtcc aggatgaaga ggatggcaca
3541 ccggctggga gagaagatgc tgaacaagaa gctgaaggat cagaaaaccc caatccccga
3601 caccctgtac caggagctgt acgactatgt gaatcacaga ctgtcccacg acctgtctga
3661 tgaggccagg gccctgctgc ccaacgtgat caccaaggag gtgtctcacg agatcatcaa
3721 ggataggcgc tttaccagcg acaagttctt tttccacgtg cctatcacac tgaactatca
3781 ggccgccaat tccccatcta agttcaacca gagggtgaat gcctacctga aggagcaccc
3841 cgagacacct atcatcggca tcgatcgggg cgagagaaac ctgatctata tcacagtgat
3901 cgactccacc ggcaagatcc tggagcagcg gagcctgaac accatccagc agtttgatta
3961 ccagaagaag ctggacaaca gggagaagga gagggtggca gcaaggcagg cctggtctgt
4021 ggtgggcaca atcaaggatc tgaagcaggg ctatctgagc caggtcatcc acgagatcgt
4081 ggacctgatg atccactacc aggccgtggt ggtgctggag aacctgaatt tcggctttaa
4141 gagcaagagg accggcatcg ccgagaaggc cgtgtaccag cagttcgaga agatgctgat
4201 cgataagctg aattgcctgg tgctgaagga ctatccagca gagaaagtgg gaggcgtgct
4261 gaacccatac cagctgacag accagttcac ctcctttgcc aagatgggca cccagtctgg
4321 cttcctgttt tacgtgcctg ccccatatac atctaagatc gatcccctga ccggcttcgt
4381 ggaccccttc gtgtggaaaa ccatcaagaa tcacgagagc cgcaagcact tcctggaggg
4441 cttcgacttt ctgcactacg acgtgaaaac cggcgacttc atcctgcact ttaagatgaa
4501 cagaaatctg tccttccaga ggggcctgcc cggctttatg cctgcatggg atatcgtgtt
4561 cgagaagaac gagacacagt ttgacgccaa gggcacccct ttcatcgccg gcaagagaat
4621 cgtgccagtg atcgagaatc acagattcac cggcagatac cgggacctgt atcctgccaa
4681 cgagctgatc gccctgctgg aggagaaggg catcgtgttc agggatggct ccaacatcct
4741 gccaaagctg ctggagaatg acgattctca cgccatcgac accatggtgg ccctgatccg
4801 cagcgtgctg cagatgcgga actccaatgc cgccacaggc gaggactata tcaacagccc
4861 cgtgcgcgat ctgaatggcg tgtgcttcga ctcccggttt cagaacccag agtggcccat
4921 ggacgccgat gccaatggcg cctaccacat cgccctgaag ggccagctgc tgctgaatca
4981 cctgaaggag agcaaggatc tgaagctgca gaacggcatc tccaatcagg actggctggc
5041 ctacatccag gagctgcgca acaaaaggcc ggcggccacg aaaaaggccg gccaggcaaa
5101 aaagaaaaag ggatcctacc catacgatgt tccagattac gcttatccct acgacgtgcc
5161 tgattatgca tacccatatg atgtccccga ctatgcctaa gaattctgca gatatccagc
5221 acagtggcgg ccgctcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt
5281 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
5341 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
5401 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
5461 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
5521 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
5581 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
5641 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
5701 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
5761 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
5821 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
5881 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
5941 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
6001 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
6061 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
6121 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
6181 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
6241 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
6301 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa
6361 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
6421 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
6481 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
6541 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
6601 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
6661 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
6721 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
6781 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
6841 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
6901 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
6961 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
7021 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
7081 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
7141 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
7201 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
7261 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
7321 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
7381 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
7441 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
7501 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
7561 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
7621 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
7681 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
7741 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
7801 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
7861 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
7921 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
7981 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
8041 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
8101 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
8161 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
8221 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
8281 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
8341 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
8401 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
8461 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
8521 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
8581 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
8641 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
8701 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
8761 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
8821 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
8881 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
8941 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
9001 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
9061 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
9121 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
9181 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
9241 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
9301 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
9361 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
9421 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
9481 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
9541 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
9601 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
9661 gaaaagtgcc acctgacgtc
//
