LOCUS pLSLZ 15655 bp DNA circular SYN 12-JUL-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 15655)
AUTHORS Biofeng
TITLE Direct Submission
JOURNAL Exported Jul 12, 2023 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..15655
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind 529..553
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 587..607
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 878..1462
/codon_start=1
/product="nonspecific DNA cleavage domain of the FokI
endonuclease (Li et al., 1992)"
/label=FokI cleavage domain
/note="must dimerize to be catalytically active"
/translation="LVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVM
EFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRY
VEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVLS
VEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
terminator 1476..1728
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
protein_bind 1773..1793
/gene="mutant version of attB"
/label=attB5
/bound_moiety="BP Clonase(TM)"
/note="core recombination site for the Gateway(R) BP
reaction"
CDS 2824..3615
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
terminator 3642..3894
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
protein_bind complement(4907..4931)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
promoter 5697..6041
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
primer_bind complement(6752..6768)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 6776..6792
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6800..6830)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6845..6866
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 7057..7734
/label=CaMV 35S promoter (enhanced)
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
CDS 7802..8827
/codon_start=1
/product="hygromycin B phosphotransferase"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
K"
polyA_signal 8868..9042
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
misc_feature 9120..9144
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
CDS 9569..10363
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
rep_origin 10450..11038
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 11224..11364
/label=bom
/note="basis of mobility region from pBR322"
rep_origin 11708..11902
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
CDS complement(11968..13035)
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
MRYRNLIEGEASAGS"
CDS complement(13469..14098)
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
misc_feature 15398..15422
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
primer_bind 15625..15641
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 cctgcaggta gcagaaggca tgttgttgtg actccgaggg gttgcctcaa actctatctt
61 ataaccggcg tggaggcatg gaggcagggg tattttggtc attttaatag atagtggaaa
121 atgacgtgga atttacttaa agacgaagtc tttgcgacaa gggggggccc acgccgaatt
181 taatattacc ggcgtggccc ccccttatcg cgagtgcttt agcacgagcg gtccagattt
241 aaagtagaaa atttcccgcc cactagggtt aaaggtgttc acactataaa agcatatacg
301 atgtgatggt atttgatgga gcgtatattg tatcaggtat ttccgttgga tacgaattat
361 tcgtacgacc ctccctaaga ttcttgattg tttataaaac caaatctcat tgtctttgtt
421 gtgtattgtt tgcaggacgt cgagagttct caacacaaca tatacaaaac aaacgaatct
481 caagcaatca agcattctac ttctattgca gcaatttaaa tcatttctac aagtttgtac
541 aaaaaagcag gctccgaatt cgcccttcac catggcttcc tcccctccaa agaaaaagag
601 aaaggttctt gaattgcctt acgcttgtcc agttgagtct tgtgatagac gttttagtag
661 gtcagacgaa ctcactaggc atattagaat ccacacagga cagaagcctt tccagtgtag
721 aatctgtatg aggaacttta gtcgtagcga tcatttgact acacatatca gaacccatac
781 tggtgagaaa ccattcgcat gtgacatctg tggtcgtaag ttcgcaagaa gtgatgaaag
841 gaagcgtcat acaaagatcc atcttcgtca gaaggaacta gtgaaatctg aattggaaga
901 gaagaaatct gaacttagac ataaattgaa atatgtgcca catgaatata ttgaattgat
961 tgaaatcgca agaaattcaa ctcaggatag aatccttgaa atgaaggtga tggagttctt
1021 tatgaaggtt tatggttatc gtggtaaaca tttgggtgga tcaaggaaac cagacggagc
1081 aatttatact gtcggatctc ctattgatta cggtgtgatc gttgatacta aggcatattc
1141 aggaggttat aatcttccaa ttggtcaagc agatgaaatg caaagatatg tcgaagagaa
1201 tcaaacaaga aacaagcata tcaaccctaa tgaatggtgg aaagtctatc catcttcagt
1261 aacagaattt aagttcttgt ttgtgagtgg tcatttcaaa ggaaactaca aagctcagct
1321 tacaagattg aatcatatca ctaattgtaa tggagctgtt cttagtgtag aagagctttt
1381 gattggtgga gaaatgatta aagctggtac attgacactt gaggaagtga gaaggaaatt
1441 taataacggt gagataaact tttaatagga cgtccgatcg ttcaaacatt tggcaataaa
1501 gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga
1561 attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt
1621 ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg
1681 caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg gaattgatcc
1741 cccctcgaca gcttccggaa agggcgaatt cgcaactttg tatacaaaag ttgccgagct
1801 cgcggccgca gtgcgagatc gggccggcct ggccctgttt tgattgatgt acctaaggat
1861 attcagcaac aattggtgat acctgactgg gatcagccaa tgaggttgcc tggttacatg
1921 tctaggttac ctaaattgcc caatgagatg cttttagaac aaattgttag gcttatttct
1981 gagtcaaaga agcctgtttt gtatgtgggg ggtgggtgtt cgcaatcgag tgaggagttg
2041 agacgattcg tggagctcac cggtatcccc gtggcaagta ctttgatggg tcttggagct
2101 tttccaactg gggatgagct ttccctttca atgttgggta tgcatggtac tgtttatgct
2161 aattatgctg tggacagtag tgatttattg ctcgcatttg gggtgaggtt tgatgataga
2221 gttactggaa agttagaagc ttttgctagc cgagcgaaaa ttgttcacat tgatattgat
2281 tcagctgaga ttggaaagaa caagcagcct catgtttcca tttgtgcgga tatcaagttg
2341 gcgttacagg gtttgaattc gatattggag agtaaggaag gtaaactgaa gttggatttt
2401 tctgcttgga ggcaggagtt gacggtgcag aaagtgaagt acccgttgaa ttttaaaact
2461 tttggtgatg ctattcctcc gcaatatgct atccaggttc tagatgagtt aactaatggg
2521 agtgctatta taagtaccgg tgttgggcag caccagatgt gggctgctca atattataag
2581 tacagaaagc cacgccaatg gttgacatct ggtggattag gagcgatggg atttggtttg
2641 cccgctgcta ttggtgcggc tgttggaaga cctgatgaag ttgtggttga cattgatggt
2701 gatggcagtt tcatcatgaa tgtgcaggag ctagcaacta ttaaggtgga gaatctccca
2761 gttaagatta tgttactgaa taatcaacac ttgggaatgg ttgtacaaat ggcttcctcc
2821 tggattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
2881 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
2941 gcgcagggta gaccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
3001 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgtaccttg cgctgctgtg
3061 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
3121 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
3181 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
3241 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
3301 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag aatgcccgac
3361 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
3421 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
3481 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
3541 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
3601 gacgagttct tctgataacc gcggagagct cgaatttccc cgatcgttca aacatttggc
3661 aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc
3721 tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat
3781 gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat
3841 agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcgtgggc
3901 ctcgaggatc gattctataa ggctaacaga gcacacacat acctggggaa tccttctaat
3961 gaggcggaga tctttcctaa tatgttgaaa tttgcagagg cttgtggcgt acctgctgcg
4021 agagtgacac acagggatga tcttagagcg gctattcaaa agatgttaga cactcctggg
4081 ccatacttgt tggatgtgat tgtacctcat caggaacatg ttctacctat gattcctacc
4141 ggaggggctt tcaaagacgt catcacagag ggtgacggga gaagttccta ttgactttga
4201 ggtgctacag agctagttct aggccttgta ttatctaaaa taaacttcta ttaaaccaaa
4261 aatgttatgt ctattagttt gttattagtt tttccgtggc tttgctcatt gtcagtgttg
4321 tactattaag tagttgatat ttatgtttgc tttaagtttt gcatcatctc gctttggttt
4381 tgaatgtgaa ggatttcagc aatgtttcat tctctattcg caacatccag tcggtatccg
4441 gagctctatg tagtatgtct ggagattaat ttctagtgga gtagtttagt gcgataaagt
4501 tagcttgttc cacattttta tttcgtaacc tgggtcagat tggaacttcc tctttaggtt
4561 ggatgcaatc cctatttggg ctttctctta atttcattat tgaaattgtt ggcttttaat
4621 ctgagcaagt tgatttgcag ctttctctct tgagtcctag cgagcaatac gttatctctg
4681 tctcctattt cttagtggat aatcttatga tggaaatctg tggagatagg aaactgttga
4741 ctgctcattc tctctttgtg aggcgtctga cagttacgct ttcaatctat agcagttcga
4801 tcagactttc tttgcttata acaatactag atacagttgg accgtgtagc acgggcccaa
4861 caatttgtgt aacgttatct ttaattcaaa atatcgcagg tcgacgaccc agctttcttg
4921 tacaaagtgg gagtgtactt caagtcagtg ggaaatcaat aaaatgatta ttttatgaat
4981 atatttcatt gtgcaagtag atagaaatta catatgttac ataacacacg aaataaacaa
5041 aaaaagacaa tccaaaaaca aacaccccaa aaaaaataat cactttagat aaactcgtat
5101 gaggagaggc acgttcagtg actcgacgat tcccgagcaa aaaaagtctc cccgtcacac
5161 atgtagtggg tgacgcaatt atctttaaag taatccttct gttgacttgt cattgataac
5221 atccagtctt cgtcaggatt gcaaagaatt atagaaggga tcccactcga gccaagcttg
5281 gcgtgtctgc aggtcaacat ggtggagcac gacacacttg tctactccaa aaatatcaaa
5341 gatacagtct cagaagacca aagggcaatt gagacttttc aacaaagggt aatatccgga
5401 aacctcctcg gattccattg cccagctatc tgtcacttta ttgtgaagat agtggaaaag
5461 gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggccatcgt tgaagatgcc
5521 tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa
5581 gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg ataacatggt ggagcacgac
5641 acacttgtct actccaaaaa tatcaaagat acagtctcag aagaccaaag ggcaattgag
5701 acttttcaac aaagggtaat atccggaaac ctcctcggat tccattgccc agctatctgt
5761 cactttattg tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca tcattgcgat
5821 aaaggaaagg ccatcgttga agatgcctct gccgacagtg gtcccaaaga tggaccccca
5881 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
5941 tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac
6001 ccttcctcta tataaggaag ttcatttcat ttggagagga cctcgactct agaggatccc
6061 ccttcctcta tataaggaag ttcatttcat ttggagaggt aagtttcact tcacacatta
6121 ttactgtctt ctaatacaag gttttttatc aagctggaga agagcatgat agtgggtagt
6181 gccatcttga tgaagctcag aagcaacacc aaggaagaaa ataagaaaag gtgtgagttt
6241 ctcccagaga aactggaata aatcatctct ttgagatgag cacttgggat aggtaaggaa
6301 aacatattta gattggagtc tgaagttctt actagcagaa ggcatgttgt tgtgactccg
6361 aggggttgcc tcaaactcta tcttataacc ggcgtggagg catggaggca ggggtatttt
6421 ggtcatttta atagatagtg gaaaatgacg tggaatttac ttaaagacga agtctttgcg
6481 acaagggggg gcccacgccg aatttaatat taccggcgtg gccccccctt atcgcgagtg
6541 ctttagcacg agcggtccag atttaaagta gaaaatttcc cgcccactag ggttaaaggt
6601 gttcacacta taaaagcata tacgatgtga tggtatttga tggagcgtat attgtatcag
6661 gtatttccgt tggatacgaa ttattcgtac gaccctccct gcaggtcgac tctagaggat
6721 ccccgggtac cgagctcgaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt
6781 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
6841 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
6901 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
6961 gtattggcta gagcagcttg ccaacatggt ggagcacgac actctcgtct actccaagaa
7021 tatcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac aaagggtaat
7081 atcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatca aaaggacagt
7141 agaaaaggaa ggtggcacct acaaatgcca tcattgcgat aaaggaaagg ctatcgttca
7201 agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga gcatcgtgga
7261 aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata acatggtgga
7321 gcacgacact ctcgtctact ccaagaatat caaagataca gtctcagaag accaaagggc
7381 tattgagact tttcaacaaa gggtaatatc gggaaacctc ctcggattcc attgcccagc
7441 tatctgtcac ttcatcaaaa ggacagtaga aaaggaaggt ggcacctaca aatgccatca
7501 ttgcgataaa ggaaaggcta tcgttcaaga tgcctctgcc gacagtggtc ccaaagatgg
7561 acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca
7621 agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc
7681 gcaagacctt cctctatata aggaagttca tttcatttgg agaggacacg ctgaaatcac
7741 cagtctctct ctacaaatct atctctctcg agctttcgca gatccggggg ggcaatgaga
7801 tatgaaaaag cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga
7861 cagcgtctcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga
7921 tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga
7981 tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat
8041 tggggagttt agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt
8101 gcaagacctg cctgaaaccg aactgcccgc tgttctacaa ccggtcgcgg aggctatgga
8161 tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg
8221 aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta
8281 tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga
8341 gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg
8401 ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc
8461 gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc
8521 ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc
8581 acgactccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga
8641 cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg
8701 agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg
8761 ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa
8821 gaaatagagt agatgccgac gcggatctgt cgatcgacaa gctcgagttt ctccataata
8881 atgtgtgagt agttcccaga taagggaatt agggttccta tagggtttcg ctcatgtgtt
8941 gagcatataa gaaaccctta gtatgtattt gtatttgtaa aatacttcta tcaataaaat
9001 ttctaattcc taaaaccaaa atccagtact aaaatccaga tcccccgaat taattcggcg
9061 ttaattcagt acattaaaaa cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg
9121 tttacaccac aatatatcct gccaccagcc agccaacagc tccccgaccg gcagctcggc
9181 acaaaatcac cactcgatac aggcagccca tcagtccggg acggcgtcag cgggagagcc
9241 gttgtaaggc ggcagacttt gctcatgtta ccgatgctat tcggaagaac ggcaactaag
9301 ctgccgggtt tgaaacacgg atgatctcgc ggagggtagc atgttgattg taacgatgac
9361 agagcgttgc tgcctgtgat caccgcggtt tcaaaatcgg ctccgtcgat actatgttat
9421 acgccaactt tgaaaacaac tttgaaaaag ctgttttctg gtatttaagg ttttagaatg
9481 caaggaacag tgaattggag ttcgtcttgt tataattagc ttcttggggt atctttaaat
9541 actgtagaaa agaggaagga aataataaat ggctaaaatg agaatatcac cggaattgaa
9601 aaaactgatc gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc ctgctaaggt
9661 atataagctg gtgggagaaa atgaaaacct atatttaaaa atgacggaca gccggtataa
9721 agggaccacc tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg aaggaaagct
9781 gcctgttcca aaggtcctgc actttgaacg gcatgatggc tggagcaatc tgctcatgag
9841 tgaggccgat ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc ctgaaaagat
9901 tatcgagctg tatgcggagt gcatcaggct ctttcactcc atcgacatat cggattgtcc
9961 ctatacgaat agcttagaca gccgcttagc cgaattggat tacttactga ataacgatct
10021 ggccgatgtg gattgcgaaa actgggaaga agacactcca tttaaagatc cgcgcgagct
10081 gtatgatttt ttaaagacgg aaaagcccga agaggaactt gtcttttccc acggcgacct
10141 gggagacagc aacatctttg tgaaagatgg caaagtaagt ggctttattg atcttgggag
10201 aagcggcagg gcggacaagt ggtatgacat tgccttctgc gtccggtcga tcagggagga
10261 tatcggggaa gaacagtatg tcgagctatt ttttgactta ctggggatca agcctgattg
10321 ggagaaaata aaatattata ttttactgga tgaattgttt tagtacctag aatgcatgac
10381 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
10441 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
10501 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
10561 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
10621 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
10681 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
10741 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
10801 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
10861 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
10921 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
10981 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
11041 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
11101 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
11161 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
11221 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
11281 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg
11341 ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga
11401 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
11461 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg
11521 gcgccggcgg tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat tgcctggccg
11581 taggccagcc atttttgagc ggccagcggc cgcgataggc cgacgcgaag cggcggggcg
11641 tagggagcgc agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc
11701 agacagttat gcacaggcca ggcgggtttt aagagtttta ataagtttta aagagtttta
11761 ggcggaaaaa tcgccttttt tctcttttat atcagtcact tacatgtgtg accggttccc
11821 aatgtacggc tttgggttcc caatgtacgg gttccggttc ccaatgtacg gctttgggtt
11881 cccaatgtac gtgctatcca caggaaagag accttttcga cctttttccc ctgctagggc
11941 aatttgccct agcatctgct ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag
12001 gttgcggtag cgcatgacta ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta
12061 ctccggcagg tcatttgacc cgatcagctt gcgcacggtg aaacagaact tcttgaactc
12121 tccggcgctg ccactgcgtt cgtagatcgt cttgaacaac catctggctt ctgccttgcc
12181 tgcggcgcgg cgtgccaggc ggtagagaaa acggccgatg ccgggatcga tcaaaaagta
12241 atcggggtga accgtcagca cgtccgggtt cttgccttct gtgatctcgc ggtacatcca
12301 atcagctagc tcgatctcga tgtactccgg ccgcccggtt tcgctcttta cgatcttgta
12361 gcggctaatc aaggcttcac cctcggatac cgtcaccagg cggccgttct tggccttctt
12421 cgtacgctgc atggcaacgt gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc
12481 tttctgcttt ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg
12541 gaacacgcgg ccgggcttgt ctcccttccc ttcccggtat cggttcatgg attcggttag
12601 atgggaaacc gccatcagta ccaggtcgta atcccacaca ctggccatgc cggccggccc
12661 tgcggaaacc tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg
12721 gtcacgcttc gacagacgga aaacggccac gtccatgatg ctgcgactat cgcgggtgcc
12781 cacgtcatag agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct
12841 aatcgacggc gcaccggctg ccggcggttg ccgggattct ttgcggattc gatcagcggc
12901 cgcttgccac gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg
12961 cgcggccttc aacttctcca ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc
13021 ggatggtttg cgaccgtcac gccgattcct cgggcttggg ggttccagtg ccattgcagg
13081 gccggcagac aacccagccg cttacgcctg gccaaccgcc cgttcctcca cacatggggc
13141 attccacggc gtcggtgcct ggttgttctt gattttccat gccgcctcct ttagccgcta
13201 aaattcatct actcatttat tcatttgctc atttactctg gtagctgcgc gatgtattca
13261 gatagcagct cggtaatggt cttgccttgg cgtaccgcgt acatcttcag cttggtgtga
13321 tcctccgccg gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc caggctggcc
13381 aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt gtttgtgctt
13441 ttgctcattt tctctttacc tcattaactc aaatgagttt tgatttaatt tcagcggcca
13501 gcgcctggac ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg gttgtgccgg
13561 cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga atgggcagct
13621 cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg atcgcccgcg
13681 acacgacaaa ggccgcttgt agccttccat ccgtgacctc aatgcgctgc ttaaccagct
13741 ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga atcagcacga
13801 agtcggctgc cttgatcgcg gacacagcca agtccgccgc ctggggcgct ccgtcgatca
13861 ctacgaagtc gcgccggccg atggccttca cgtcgcggtc aatcgtcggg cggtcgatgc
13921 cgacaacggt tagcggttga tcttcccgca cggccgccca atcgcgggca ctgccctggg
13981 gatcggaatc gactaacaga acatcggccc cggcgagttg cagggcgcgg gctagatggg
14041 ttgcgatggt cgtcttgcct gacccgcctt tctggttaag tacagcgata accttcatgc
14101 gttccccttg cgtatttgtt tatttactca tcgcatcata tacgcagcga ccgcatgacg
14161 caagctgttt tactcaaata cacatcacct ttttagacgg cggcgctcgg tttcttcagc
14221 ggccaagctg gccggccagg ccgccagctt ggcatcagac aaaccggcca ggatttcatg
14281 cagccgcacg gttgagacgt gcgcgggcgg ctcgaacacg tacccggccg cgatcatctc
14341 cgcctcgatc tcttcggtaa tgaaaaacgg ttcgtcctgg ccgtcctggt gcggtttcat
14401 gcttgttcct cttggcgttc attctcggcg gccgccaggg cgtcggcctc ggtcaatgcg
14461 tcctcacgga aggcaccgcg ccgcctggcc tcggtgggcg tcacttcctc gctgcgctca
14521 agtgcgcggt acagggtcga gcgatgcacg ccaagcagtg cagccgcctc tttcacggtg
14581 cggccttcct ggtcgatcag ctcgcgggcg tgcgcgatct gtgccggggt gagggtaggg
14641 cgggggccaa acttcacgcc tcgggccttg gcggcctcgc gcccgctccg ggtgcggtcg
14701 atgattaggg aacgctcgaa ctcggcaatg ccggcgaaca cggtcaacac catgcggccg
14761 gccggcgtgg tggtgtcggc ccacggctct gccaggctac gcaggcccgc gccggcctcc
14821 tggatgcgct cggcaatgtc cagtaggtcg cgggtgctgc gggccaggcg gtctagcctg
14881 gtcactgtca caacgtcgcc agggcgtagg tggtcaagca tcctggccag ctccgggcgg
14941 tcgcgcctgg tgccggtgat cttctcggaa aacagcttgg tgcagccggc cgcgtgcagt
15001 tcggcccgtt ggttggtcaa gtcctggtcg tcggtgctga cgcgggcata gcccagcagg
15061 ccagcggcgg cgctcttgtt catggcgtaa tgtctccggt tctagtcgca agtattctac
15121 tttatgcgac taaaacacgc gacaagaaaa cgccaggaaa agggcagggc ggcagcctgt
15181 cgcgtaactt aggacttgtg cgacatgtcg ttttcagaag acggctgcac tgaacgtcag
15241 aagccgactg cactatagca gcggaggggt tggatcaaag tactttgatc ccgaggggaa
15301 ccctgtggtt ggcatgcaca tacaaatgga cgaacggata aaccttttca cgccctttta
15361 aatatccgtt attctaataa acgctctttt ctcttaggtt tacccgccaa tatatcctgt
15421 caaacactga tagtttaaac tgaaggcggg aaacgacaat ctgatccaag ctcaagctgc
15481 tctagcattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
15541 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
15601 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctt gcatg
//
