| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KAT6B-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001613 |
| 产品用途: | 3' AAVLINK plasmid for KAT6B expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | KAT6B |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KAT6B-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KAT6B-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KAT6B-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KAT6B-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KAT6B-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KAT6B-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KAT6B-FLAG 7301 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7301)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 20, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7301
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..4014
/codon_start=1
/label=KAT6B
/translation="EQSAVYLSGGKTKRGRPDLN*ICTPRQKHPWSLTSR*QWKNRRRL
QKEKPAPVPSGLRRRSRKLGKPCCLKRKTEGKKHVPL*VQTHHQVKNQKMISSNLRKRK
RRRRRKRKKRKKRKGKKKKEEEM*KKIQMVLKAKKKRNQKSPRKKKTLHVWMITKRRRK
RMKSHPTTRTMMPMTRMTATWSLPKWRRKSCPEKASKKYWKTRRLF*TLMCSLVTRTQR
S*WTVAST*QLLVTVSPRSLLGTLKLYPNLTRSHPQENRHRSRTKRTARKSIQSSKRET
QQPWKSTLRLSRPFSL*PRRAANRTTPFRIVPRLKRPVEAYRTTPVQTKVHRLPPRSTI
ANSRTTVAQFHPSTPILASPYVLSTAQVSLLWKTATPKSAQIKVPSQCHLCRTWKPVP*
WMSHQFQIIHSKS*TVDLVTWAVSRAQLRTTKTQAATILLWEAASVETALHRTAAPIAT
SPPAV*HRAAVLSPSRCPTSAGAAACCSKPASAPLRPAASSLLKAVWWRGLRAAASSWL
SAAWLLTSPHPCSWLKSPRRATPTLAYTSEWVRVILGLGITRSRQPPSALPNCSS*LIH
LLIIHCLTAIPLL*LPMQTVPLCPHH*VTQGLFNFLSLHTPSLGDPKHKLP*PHPPT*L
LLQ*ICRRLFCNGTWLHQILASLTAKDCKPRLPARATSP*EPSQRLCHQPLPPISHKSM
GAPRL*PCRVLHGL*RCKEA*T*V*T*CQRQPTMSTL*T*T*TLSTP*MGTACPSQ**T
VATTAIMAI*IKRPNTLCRCRWA*WAPSHMPSSQCRPHPTVT*CTRPPDITAT*TQACP
NSLSMAPT*E"
misc_feature 1718..1723
/label=SDS
misc_feature 1721..1726
/label=SDS
misc_feature 1859..1864
/label=SDS
misc_feature 1862..1867
/label=SDS
misc_feature 1865..1870
/label=SDS
misc_feature 1868..1873
/label=SDS
misc_feature 1914..1919
/label=SDS
misc_feature 1917..1922
/label=SDS
misc_feature 2024..2029
/label=SDS
misc_feature 2027..2032
/label=SDS
misc_feature 3451..3456
/label=SDS
CDS 4021..4086
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 4090..4521
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4234..4239
/label=SDS
repeat_region 4564..4704
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4564..4693
/label=AAV2 ITR
rep_origin 4779..5234
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5516..5620
/gene="bla"
/label=AmpR promoter
CDS 5621..6481
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6652..7240
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggaac aaagcgcggt
1561 ctatctaagt ggaggcaaaa caaagagagg aagaccggat ttaaactgaa tttgtacacc
1621 ccgccagaaa cacccatgga gcctgacgag caggtaacag tggaagaaca gaaggagact
1681 tcagaaggaa aaaccagccc cagtcccatc aggattgagg aggaggtcaa ggaaactggg
1741 gaagccctgt tgcctcaaga ggaaaacaga agggaagaaa catgtgcccc tgtaagtcca
1801 aacacatcac caggtgaaaa accagaagat gatctcatca aacctgagga agaggaagag
1861 gaggaggagg aggaagagga agaagaggaa gaagaggaag gggaagaaga agaaggagga
1921 ggaaatgtag aaaaagatcc agatggtgct aaaagccaag aaaaagagga accagaaatc
1981 tccacggaaa aagaagactc tgcacgtttg gatgatcacg aagaggagga ggaagaggat
2041 gaagagccat cccacaacga ggaccatgat gccgatgacg aggatgacag ccacatggag
2101 tctgccgaag tggagaagga agagctgccc agagaaagct tcaaagaagt actggaaaac
2161 caggagactt ttttagacct taatgtgcag cctggtcact cgaacccaga ggtcttaatg
2221 gactgtggcg tcgacctgac agcttcttgt aacagtgagc ccaaggagct tgctggggac
2281 cctgaagctg tacccgaatc tgacgaggag ccacccccag gagaacaggc acagaagcag
2341 gaccaaaaga acagcaagga agtcgataca gagttcaaag agggaaaccc agcaaccatg
2401 gaaatcgact ctgagactgt ccaggccgtt cagtctttga cccaggagag cagcgaacag
2461 gacgacacct ttcaggattg tgccgagact caagaggcct gtagaagcct acagaactac
2521 acccgtgcag accaaagtcc acagattgcc accacgctcg acgattgcca acagtcggac
2581 cacagtagcc cagtttcatc cgtccactcc catcctggcc agtccgtacg ttctgtcaac
2641 agcccaagtg tccctgctct ggaaaacagc tacgcccaaa tcagcccaga tcaaagtgcc
2701 atctcagtgc catctctgca gaacatggaa accagtccca tgatggatgt cccatcagtt
2761 tcagatcatt cacagcaagt cgtagacagt ggatttagtg acctgggcag tatcgagagc
2821 acaactgaga actacgaaaa cccaagcagc tacgattcta ctatgggagg cagcatctgt
2881 ggaaacggct cttcacagaa cagctgctcc tatagcaacc tcacctccag cagtctgaca
2941 cagagcagct gtgctgtcac ccagcagatg tccaacatca gcgggagctg cagcatgctg
3001 cagcaaacca gcatcagctc ccctccgacc tgcagcgtca agtctcctca aggctgtgtg
3061 gtggagaggc ctccgagcag cagccagcag ctggctcagt gcagcatggc tgctaacttc
3121 accccaccca tgcagctggc tgaaatcccc gagacgagca acgccaacat tggcttatac
3181 gagcgaatgg gtcagagtga ttttggggct gggcattacc cgcagccgtc agccaccttc
3241 agccttgcca aactgcagca gttaactaat acacttattg atcattcatt gccttacagc
3301 cattccgctg ctgtgacttc ctatgcaaac agtgcctctt tgtccacacc attaagtaac
3361 acagggcttg ttcaactttc tcagtctcca cactccgtcc ctgggggacc ccaagcacaa
3421 gctaccatga ccccaccccc caacctgact cctcctccaa tgaatctgcc gccgcctctt
3481 ttgcaacgga acatggctgc atcaaatatt ggcatctctc acagccaaag actgcaaacc
3541 cagattgcca gcaagggcca catctccatg agaaccaagt cagcgtctct gtcaccagcc
3601 gctgccaccc atcagtcaca aatctatggg cgctcccaga ctgtagccat gcagggtcct
3661 gcacggactt taacgatgca aagaggcatg aacatgagtg tgaacctgat gccagcgcca
3721 gcctacaatg tcaactctgt gaacatgaac atgaacactc tcaacgccat gaatgggtac
3781 agcatgtccc agccaatgat gaacagtggc taccacagca atcatggcta tatgaatcaa
3841 acgccccaat accctatgca gatgcagatg ggcatgatgg gcacccagcc atatgcccag
3901 cagccaatgc agaccccacc ccacggtaac atgatgtaca cggcccccgg acatcacggc
3961 tacatgaaca caggcatgtc caaacagtct ctcaatggct cctacatgag aaggtgtaca
4021 gactacaaag accatgacgg tgattataaa gatcatgaca tcgactacaa ggatgacgat
4081 gacaagtaac tcgagataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
4141 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
4201 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttagttc
4261 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
4321 tgggcactga caattccgtg gtgtttattt gtgaaatttg tgatgctatt gctttatttg
4381 taaccatcta gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
4441 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
4501 gagatgtggg aggtttttta aagatctgga gaccggtgtg gcacgtgcgg accgagcggc
4561 cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg
4621 aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg
4681 agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt
4741 gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt
4801 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccttagc
4861 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
4921 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
4981 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
5041 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
5101 aacactcaac tctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggt
5161 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
5221 aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag
5281 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
5341 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
5401 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
5461 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
5521 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
5581 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
5641 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
5701 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
5761 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
5821 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
5881 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
5941 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
6001 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
6061 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
6121 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
6181 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
6241 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
6301 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
6361 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
6421 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
6481 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
6541 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
6601 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
6661 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
6721 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
6781 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
6841 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
6901 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
6961 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
7021 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
7081 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
7141 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
7201 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
7261 tttacggttc ctggcctttt gctggccttt tgctcacatg t
//
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KAT6B-FLAG载体图谱质粒图谱pdf版和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KAT6B-FLAG载体序列质粒序列等相关资料下载
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KAT6B-FLAG质粒载体应用举例
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