| 质粒名称: | mIL12-N1 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 123139 |
| 存储实验室: | Jordan Green |
|
载体骨架基本信息 |
|
| 载体名称: | N1 |
| 载体出品公司: | clonTech |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 4000 bp |
| 完整质粒大小: | 5685 bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Murine IL12 tandem p40 p35,mIL-12,p40,p35 |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 1773 bp |
| GenBank编号 | 16159 16160 |
| 启动子 | CMV |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | NotI(未破坏) |
| 3' 克隆位点: | PvuI (未破坏) |
| 5' 测序引物 | cgcaaatgggcggtaggcgtg |
| 3' 测序引物 | GATGAGTTTGGACAAACCAC |
|
文献引用方法 |
|
| 材料和方法部分: | mIL12-N1 was a gift from Jordan Green (Addgene plasmid # 123139 ; http://n2t.net/addgene:123139 ; RRID:Addgene_123139) |
| 参考文献部分: | In situ genetic engineering of tumors for long-lasting and systemic immunotherapy. Tzeng SY, Patel KK, Wilson DR, Meyer RA, Rhodes KR, Green JJ. Proc Natl Acad Sci U S A. 2020 Feb 7. pii: 1916039117. doi: 10.1073/pnas.1916039117. 10.1073/pnas.1916039117 PubMed 32034097 |
买家导航
mIL12N1载体基本信息
mIL12N1载体图谱质粒图谱和多克隆位点信息
mIL12N1载体简介
mIL12N1载体序列
LOCUS mIL12-N1 5685 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Mammalian expression of murine cytokine IL-12 under CMV promoter.
ACCESSION .
VERSION .
KEYWORDS mIL12-N1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5685)
AUTHORS Tzeng SY, Patel KK, Wilson DR, Meyer RA, Rhodes KR, Green JJ
TITLE In situ genetic engineering of tumors for long-lasting and systemic
immunotherapy.
JOURNAL Proc Natl Acad Sci U S A. 2020 Feb 7. pii: 1916039117. doi:
10.1073/pnas.1916039117.
PUBMED 32034097
REFERENCE 2 (bases 1 to 5685)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5685
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 1..304
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 305..508
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 459..479
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
polyA_signal 2413..2534
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2450..2469)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 2504..2523
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(2541..2996)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2678..2699)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 2890..2909
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 3023..3127
/gene="bla"
/label=AmpR promoter
promoter 3129..3486
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(3152..3172)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 3337..3472
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3399..3418
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 3521..4315
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3575..3594)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 4185..4204
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(4503..4522)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 4547..4594
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4923..5511
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5412..5431
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
541 ccggactcag atctcgagct caagcttggt accgagctcg gatcgatatc tgcggccgcg
601 tcgccaccat gtgtcctcag aagctaacca tctcctggtt tgccatcgtt ttgctggtgt
661 ctccactcat ggccatgtgg gagctggaga aagacgttta tgttgtagag gtggactgga
721 ctcccgatgc ccctggagaa acagtgaacc tcacctgtga cacgcctgaa gaagatgaca
781 tcacctggac ctcagaccag agacatggag tcataggctc tggaaagacc ctgaccatca
841 ctgtcaaaga gtttctagat gctggccagt acacctgcca caaaggaggc gagactctga
901 gccactcaca tctgctgctc cacaagaagg aaaatggaat ttggtccact gaaattttaa
961 aaaatttcaa aaacaagact ttcctgaagt gtgaagcacc aaattactcc ggacggttca
1021 cgtgctcatg gctggtgcaa agaaacatgg acttgaagtt caacatcaag agcagtagca
1081 gttcccctga ctctcgggca gtgacatgtg gaatggcgtc tctgtctgca gagaaggtca
1141 cactggacca aagggactat gagaagtatt cagtgtcctg ccaggaggat gtcacctgcc
1201 caactgccga ggagaccctg cccattgaac tggcgttgga agcacggcag cagaataaat
1261 atgagaacta cagcaccagc ttcttcatca gggacatcat caaaccagac ccgcccaaga
1321 acttgcagat gaagcctttg aagaactcac aggtggaggt cagctgggag taccctgact
1381 cctggagcac tccccattcc tacttctccc tcaagttctt tgttcgaatc cagcgcaaga
1441 aagaaaagat gaaggagaca gaggaggggt gtaaccagaa aggtgcgttc ctcgtagaga
1501 agacatctac cgaagtccaa tgcaaaggcg ggaatgtctg cgtgcaagct caggatcgct
1561 attacaattc ctcatgcagc aagtgggcat gtgttccctg cagggtccga tccggcggcg
1621 gcgggagtgg cggcgggggt tctggcggag gcctcgctag cggtggctcc atggtcagcg
1681 ttccaacagc ctcaccctcg gcatccagca gctcctctca gtgccggtcc agcatgtgtc
1741 aatcacgcta cctcctcttt ttggccaccc ttgccctcct aaaccacctc agtttggcca
1801 gggtcattcc agtctctgga cctgccaggt gtcttagcca gtcccgaaac ctgctgaaga
1861 ccacagatga catggtgaag acggccagag aaaaactgaa acattattcc tgcactgctg
1921 aagacatcga tcatgaagac atcacacggg accaaaccag cacattgaag acctgtttac
1981 cactggaact acacaagaac gagagttgcc tggctactag agagacttct tccacaacaa
2041 gagggagctg cctgccccca cagaagacgt ctttgatgat gaccctgtgc cttggtagca
2101 tctatgagga cttgaagatg taccagacag agttccaggc catcaacgca gcacttcaga
2161 atcacaacca tcagcagatc attctagaca agggcatgct ggtggccatc gatgagctga
2221 tgcagtctct gaatcataat ggcgagactc tgcgccagaa acctcctgtg ggagaagcag
2281 acccttacag agtgaaaatg aagctctgca tcctgcttca cgccttcagc acccgcgtcg
2341 tgaccatcaa cagggtgatg ggctatctga gctccgcctg agttaacgcg atcgcacgga
2401 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
2461 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
2521 atcaatgtat cttaaggcgt aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
2581 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
2641 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
2701 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
2761 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
2821 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
2881 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
2941 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg
3001 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
3061 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
3121 gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag
3181 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
3241 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
3301 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
3361 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
3421 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
3481 tgcaaagatc gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
3541 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
3601 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
3661 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct
3721 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
3781 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
3841 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
3901 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
3961 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
4021 agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac
4081 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
4141 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
4201 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
4261 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
4321 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat
4381 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
4441 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccctag ggggaggcta
4501 actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga
4561 cagaataaaa cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc
4621 tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc
4681 ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg
4741 gcggcaggcc ctgccatagc ctcaggttac tcatatatac tttagattga tttaaaactt
4801 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
4861 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
4921 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
4981 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
5041 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
5101 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
5161 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
5221 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
5281 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
5341 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
5401 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
5461 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
5521 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
5581 gcgttatccc ctgattctgt ggataaccgt attaccgcca tgcattagtt attaatagta
5641 atcaattacg gggtcattag ttcatagccc atatatggag ttccg
//
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